Restriction map for
pFN21AE4542
(Circular) (Six-base) MAPSORT of: pFN21AE4542.seq Check: 5000 from: 1 to: 6583
pFN21AE4542 6583 bp
With 182 enzymes: SgfI *
March 28, 2012 14:11 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2373 2760 4393 278
Size: 53 83 186 936 837 387 1633 2468
Fragments arranged by size:
2468 1633 936 837 387 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2606 2733 2820 3926 1937
Size: 669 127 87 1106 4594
Fragments arranged by size:
4594 1106 669 127 87
Acc65I G'GTAC_C
Cuts at: 3904 3904
Size: 6583
AclI AA'CG_TT
Cuts at: 1563 4710 5083 1563
Size: 3147 373 3063
Fragments arranged by size:
3147 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2480 4646 6030 6298 1632
Size: 848 2166 1384 268 1917
Fragments arranged by size:
2166 1917 1384 848 268
AfeI AGC'GCT
Cuts at: 2124 2124
Size: 6583
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6386
AflIII A'CryG_T
Cuts at: 1203 5482 6459 1203
Size: 4279 977 1327
Fragments arranged by size:
4279 1327 977
AgeI A'CCGG_T
Cuts at: 5414 5414
Size: 6583
AhdI GACnn_n'nnGTC
Cuts at: 5312 5312
Size: 6583
AleI CACnn'nnGTG
Cuts at: 1166 2166 1166
Size: 1000 5583
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2736 1184
Size: 1552 5031
AlwNI CAG_nnn'CTG
Cuts at: 2347 3613 3841 4326 5898 2347
Size: 1266 228 485 1572 3032
Fragments arranged by size:
3032 1572 1266 485 228
ApaLI G'TGCA_C
Cuts at: 4640 5796 4640
Size: 1156 5427
ApoI r'AATT_y
Cuts at: 1493 3088 3892 4118 4270 6379 1493
Size: 1595 804 226 152 2109 1697
Fragments arranged by size:
2109 1697 1595 804 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5137 160
Size: 872 4105 1606
Fragments arranged by size:
4105 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6583
AvaI C'yCGr_G
Cuts at: 1870 1943 3699 3908 1870
Size: 73 1756 209 4545
Fragments arranged by size:
4545 1756 209 73
BamHI G'GATC_C
Cuts at: 3913 4280 3913
Size: 367 6216
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2405 2841 3904 5359 618
Size: 325 217 627 618 436 1063 1455 1842
Fragments arranged by size:
1842 1455 1063 627 618 436 325 217
BanII G_rGCy'C
Cuts at: 1391 2253 2439 2481 2541 2553 3177 3189 3216
Size: 862 186 42 60 12 624 12 27
Cuts at: 3216 3243 3255 3282 3470 3902 1391
Size: 27 12 27 188 432 4072
Fragments arranged by size:
4072 862 624 432 188 186 60 42
27 27 27 12 12 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2468 928
Size: 971 569 5043
Fragments arranged by size:
5043 971 569
BbvCI CC'TCA_GC
Cuts at: 3811 3811
Size: 6583
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4774 4808 1903
Size: 34 50 34 2753 34 3678
Fragments arranged by size:
3678 2753 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4475 5685 4475
Size: 1210 5373
BclI T'GATC_A
Cuts at: 1548 3339 3421 1548
Size: 1791 82 4710
Fragments arranged by size:
4710 1791 82
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2476 5194 136
Size: 107 122 71 631 605 804 2718 1525
Fragments arranged by size:
2718 1525 804 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6578 6578
Size: 6583
BlpI GC'TnA_GC
Cuts at: 3581 3845 4000 3581
Size: 264 155 6164
Fragments arranged by size:
6164 264 155
Bme1580I G_kGCm'C
Cuts at: 1790 2410 2846 3699 4644 5800 1790
Size: 620 436 853 945 1156 2573
Fragments arranged by size:
2573 1156 945 853 620 436
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6583
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4314 5272 442
Size: 416 53 83 399 24 2897 958 1753
Fragments arranged by size:
2897 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6583
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3262 3262
Size: 6583
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2115 2246 2522 2585 3230 3454 3686 3730
Size: 876 131 276 63 645 224 232 44
Cuts at: 3730 5243 1239
Size: 1513 2579
Fragments arranged by size:
2579 1513 876 645 276 232 224 131
63 44
Bpu10I CC'TnA_GC
Cuts at: 3811 3811
Size: 6583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4708 5573 5871 4708
Size: 865 298 5420
Fragments arranged by size:
5420 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2074 3542 5246 882
Size: 617 575 1468 1704 2219
Fragments arranged by size:
2219 1704 1468 617 575
BsaAI yAC'GTr
Cuts at: 493 1204 2737 4374 493
Size: 711 1533 1637 2702
Fragments arranged by size:
2702 1637 1533 711
BsaBI GATnn'nnATC
Cuts at: 6577 6577
Size: 6583
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2370 2486 2757 4390
Size: 53 83 186 936 837 116 271 1633
Cuts at: 4390 4772 5407 275
Size: 382 635 1451
Fragments arranged by size:
1633 1451 936 837 635 382 271 186
116 83 53
BsaWI w'CCGG_w
Cuts at: 5016 5414 5688 5835 6246 5016
Size: 398 274 147 411 5353
Fragments arranged by size:
5353 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2174 2204 3420 3450 1178
Size: 30 165 30 771 30 1216 30 4311
Fragments arranged by size:
4311 1216 771 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2003 2006 2576 3013 3500 1186
Size: 817 3 570 437 487 4269
Fragments arranged by size:
4269 817 570 487 437 3
BseYI C'CCAG_C
Cuts at: 2287 2546 3260 3585 5786 6282 2287
Size: 259 714 325 2201 496 2588
Fragments arranged by size:
2588 2201 714 496 325 259
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2810 1163
Size: 573 1074 4936
Fragments arranged by size:
4936 1074 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4033 4794 5822 1540
Size: 275 186 2032 761 1028 2301
Fragments arranged by size:
2301 2032 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2253 2439 2541 3450 3470 3851 4644 4729 5800
Size: 186 102 909 20 381 793 85 1071
Cuts at: 5800 2253
Size: 3036
Fragments arranged by size:
3036 1071 909 793 381 186 102 85
20
BsmI GAATG_Cn'
Cuts at: 4094 4187 4094
Size: 93 6490
BsmBI CGTCTCn'nnnn_
Cuts at: 2377 2377
Size: 6583
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2253 2410 2439 2481 2541 2553 2846
Size: 399 463 157 29 42 60 12 293
Cuts at: 2846 3177 3189 3216 3243 3255 3282 3450 3470
Size: 331 12 27 27 12 27 168 20
Cuts at: 3470 3699 3851 3902 4644 4729 5800 1391
Size: 229 152 51 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 463 399 331 293 229
168 157 152 85 60 51 42 29
27 27 27 20 12 12 12
BspEI T'CCGG_A
Cuts at: 6246 6246
Size: 6583
BspHI T'CATG_A
Cuts at: 4472 6202 4472
Size: 1730 4853
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2145 3849 3938 844
Size: 1301 1704 89 3489
Fragments arranged by size:
3489 1704 1301 89
BsrBI CCG'CTC
Cuts at: 1394 1823 3744 4470 1394
Size: 429 1921 726 3507
Fragments arranged by size:
3507 1921 726 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2458 5078 5252 65
Size: 1183 1210 2620 174 1396
Fragments arranged by size:
2620 1396 1210 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2382 5227 5414 1700
Size: 110 572 2845 187 2869
Fragments arranged by size:
2869 2845 572 187 110
BsrGI T'GTAC_A
Cuts at: 96 2181 96
Size: 2085 4498
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6583
BssSI C'ACGA_G
Cuts at: 4643 5655 4643
Size: 1012 5571
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2326 2347 794
Size: 1532 21 5030
Fragments arranged by size:
5030 1532 21
BstBI TT'CG_AA
Cuts at: 5387 5387
Size: 6583
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6583
BstXI CCAn_nnnn'nTGG
Cuts at: 2270 3078 3664 2270
Size: 808 586 5189
Fragments arranged by size:
5189 808 586
BstYI r'GATC_y
Cuts at: 1972 2299 2688 3913 4280 4665 4682 6134 6220
Size: 327 389 1225 367 385 17 1452 86
Cuts at: 6220 6232 6578 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1225 389 385 367 346 327
86 17 12
BstZ17I GTA'TAC
Cuts at: 2607 2607
Size: 6583
BtgI C'CryG_G
Cuts at: 513 1065 2659 3439 513
Size: 552 1594 780 3657
Fragments arranged by size:
3657 1594 780 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3283 487
Size: 683 56 2057 3787
Fragments arranged by size:
3787 2057 683 56
BtsI GCAGTG_nn'
Cuts at: 2345 2798 2994 4099 4893 4913 2345
Size: 453 196 1105 794 20 4015
Fragments arranged by size:
4015 1105 794 453 196 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6583
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2916 2951 529
Size: 35 2352 35 4161
Fragments arranged by size:
4161 2352 35 35
DraI TTT'AAA
Cuts at: 3887 3887
Size: 6583
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5590 809
Size: 507 4274 1802
Fragments arranged by size:
4274 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2781 3622
Size: 54 1425 50 275 18 249 702 841
Cuts at: 3622 3775 3871 4030 4919 6548 8
Size: 153 96 159 889 1629 43
Fragments arranged by size:
1629 1425 889 841 702 275 249 159
153 96 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4030 1537
Size: 275 2218 4090
Fragments arranged by size:
4090 2218 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2889 3545 3719 3758 4513 1353
Size: 1536 656 174 39 755 3423
Fragments arranged by size:
3423 1536 755 656 174 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5170 5544 5690 387
Size: 1064 138 3581 374 146 1280
Fragments arranged by size:
3581 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2251 2437 2539 3468 2251
Size: 186 102 929 5366
Fragments arranged by size:
5366 929 186 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2115 2246 2480 2522 2585 3230 3454
Size: 393 483 131 234 42 63 645 224
Cuts at: 3454 3686 3730 4646 5243 6030 6298 1239
Size: 232 44 916 597 787 268 1524
Fragments arranged by size:
1524 916 787 645 597 483 393 268
234 232 224 131 63 44 42
EcoO109I rG'GnC_Cy
Cuts at: 2292 2631 3610 3785 3807 3838 2292
Size: 339 979 175 22 31 5037
Fragments arranged by size:
5037 979 339 175 31 22
EcoRI G'AATT_C
Cuts at: 3892 4270 3892
Size: 378 6205
FspI TGC'GCA
Cuts at: 5089 5089
Size: 6583
HaeII r_GCGC'y
Cuts at: 1678 2126 5730 1678
Size: 448 3604 2531
Fragments arranged by size:
3604 2531 448
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2284 2316 3811 3843 5224
Size: 32 135 32 712 32 1495 32 1381
Cuts at: 5224 5256 5298 5330 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1495 1381 712 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3927 4173 669
Size: 988 281 1989 246 3079
Fragments arranged by size:
3079 1989 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6583
HpaI GTT'AAC
Cuts at: 4173 4173
Size: 6583
KpnI G_GTAC'C
Cuts at: 3908 3908
Size: 6583
MfeI C'AATT_G
Cuts at: 4182 4182
Size: 6583
MluI A'CGCG_T
Cuts at: 6459 6459
Size: 6583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3059 5697 5881 1305
Size: 1754 2638 184 2007
Fragments arranged by size:
2638 2007 1754 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2081 2783 3624 3873 10
Size: 54 1425 343 249 702 841 249 2720
Fragments arranged by size:
2720 1425 841 702 343 249 249 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2166 3076 3320 4541 4900 5059 518
Size: 648 1000 910 244 1221 359 159 2042
Fragments arranged by size:
2042 1221 1000 910 648 359 244 159
MspA1I CmG'CkG
Cuts at: 1606 2230 2317 2546 3999 4676 5824 6069 1606
Size: 624 87 229 1453 677 1148 245 2120
Fragments arranged by size:
2120 1453 1148 677 624 245 229 87
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6031
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6583
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6583
NotI GC'GGCC_GC
Cuts at: 4030 4030
Size: 6583
NspI r_CATG'y
Cuts at: 1132 1229 3599 3941 5486 1132
Size: 97 2370 342 1545 2229
Fragments arranged by size:
2370 2229 1545 342 97
PasI CC'CwG_GG
Cuts at: 1348 2474 2970 3517 1348
Size: 1126 496 547 4414
Fragments arranged by size:
4414 1126 547 496
PciI A'CATG_T
Cuts at: 5482 5482
Size: 6583
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6583
PfoI T'CCnGG_A
Cuts at: 2364 3180 2364
Size: 816 5767
PmeI GTTT'AAAC
Cuts at: 3887 3887
Size: 6583
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4652 4684 6165 6197 4652
Size: 32 1481 32 5038
Fragments arranged by size:
5038 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2292 2631 3838 2292
Size: 339 1207 5037
Fragments arranged by size:
5037 1207 339
PshAI GACnn'nnGTC
Cuts at: 1613 2936 1613
Size: 1323 5260
PsiI TTA'TAA
Cuts at: 4153 4153
Size: 6583
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2165 2197 2165
Size: 32 6551
PstI C_TGCA'G
Cuts at: 830 2715 3675 3935 830
Size: 1885 960 260 3478
Fragments arranged by size:
3478 1885 960 260
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6583
PvuII CAG'CTG
Cuts at: 2230 2317 2230
Size: 87 6496
SacI G_AGCT'C
Cuts at: 2253 2439 2541 3470 2253
Size: 186 102 929 5366
Fragments arranged by size:
5366 929 186 102
SalI G'TCGA_C
Cuts at: 1936 3925 1936
Size: 1989 4594
SanDI GG'GwC_CC
Cuts at: 2292 2631 2292
Size: 339 6244
SapI GCTCTTCn'nnn_
Cuts at: 3719 3719
Size: 6583
SbfI CC_TGCA'GG
Cuts at: 3935 3935
Size: 6583
ScaI AGT'ACT
Cuts at: 4831 6543 4831
Size: 1712 4871
SfcI C'TryA_G
Cuts at: 826 1045 2711 3671 3931 5066 5747 5938 826
Size: 219 1666 960 260 1135 681 191 1471
Fragments arranged by size:
1666 1471 1135 960 681 260 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6583
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6583
SmaI CCC'GGG
Cuts at: 1872 3701 3910 1872
Size: 1829 209 4545
Fragments arranged by size:
4545 1829 209
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4687 5588 5850 820
Size: 197 926 2744 901 262 1553
Fragments arranged by size:
2744 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2737 4374 493
Size: 2244 1637 2702
Fragments arranged by size:
2702 2244 1637
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6583
SphI G_CATG'C
Cuts at: 1132 3941 1132
Size: 2809 3774
SspI AAT'ATT
Cuts at: 5 52 4507 5
Size: 47 4455 2081
Fragments arranged by size:
4455 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6583
StyI C'CwwG_G
Cuts at: 513 1065 2851 3451 3625 4022 513
Size: 552 1786 600 174 397 3074
Fragments arranged by size:
3074 1786 600 552 397 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2509 4610 4627 4780 4965 5410 325
Size: 1255 929 2101 17 153 185 445 1498
Fragments arranged by size:
2101 1498 1255 929 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 4829 6541
Size: 274 80 33 51 1443 204 2648 1712
Cuts at: 6541 96
Size: 138
Fragments arranged by size:
2648 1712 1443 274 204 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2734 2766 3092 3124 3420 3452 3672 3704 6543
Size: 32 326 32 296 32 220 32 2839
Cuts at: 6543 6575 2734
Size: 32 2742
Fragments arranged by size:
2839 2742 326 296 220 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6583
XbaI T'CTAG_A
Cuts at: 3004 3919 3004
Size: 915 5668
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2497 2932 2497
Size: 435 6148
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6583
XmaI C'CCGG_G
Cuts at: 1870 3699 3908 1870
Size: 1829 209 4545
Fragments arranged by size:
4545 1829 209
XmnI GAAnn'nnTTC
Cuts at: 4712 4712
Size: 6583
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2371 2758 4391 276
Size: 53 83 186 936 837 387 1633 2468
Fragments arranged by size:
2468 1633 936 837 387 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI RsrII SacII SexAI SfiI SfoI SrfI
SwaI