Restriction map for
pFN21AE4538
(Circular) (Six-base) MAPSORT of: pFN21AE4538.seq Check: 2803 from: 1 to: 6565
pFN21AE4538 6565 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4375 278
Size: 53 83 186 936 2839 2468
Fragments arranged by size:
2839 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3534 3908 1937
Size: 1597 374 4594
Fragments arranged by size:
4594 1597 374
Acc65I G'GTAC_C
Cuts at: 3681 3886 3681
Size: 205 6360
AclI AA'CG_TT
Cuts at: 1563 4692 5065 1563
Size: 3129 373 3063
Fragments arranged by size:
3129 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2193 2207 2387 2784 3105 3380 3697 3776
Size: 561 14 180 397 321 275 317 79
Cuts at: 3776 4628 6012 6280 1632
Size: 852 1384 268 1917
Fragments arranged by size:
1917 1384 852 561 397 321 317 275
268 180 79 14
AfeI AGC'GCT
Cuts at: 2826 3403 2826
Size: 577 5988
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6368
AflIII A'CryG_T
Cuts at: 1203 2254 2479 5464 6441 1203
Size: 1051 225 2985 977 1327
Fragments arranged by size:
2985 1327 1051 977 225
AgeI A'CCGG_T
Cuts at: 5396 5396
Size: 6565
AhdI GACnn_n'nnGTC
Cuts at: 5294 5294
Size: 6565
AleI CACnn'nnGTG
Cuts at: 1166 2321 1166
Size: 1155 5410
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6565
AlwNI CAG_nnn'CTG
Cuts at: 2365 3584 4308 5880 2365
Size: 1219 724 1572 3050
Fragments arranged by size:
3050 1572 1219 724
ApaLI G'TGCA_C
Cuts at: 4622 5778 4622
Size: 1156 5409
ApoI r'AATT_y
Cuts at: 1493 2403 3284 3874 4100 4252 6361 1493
Size: 910 881 590 226 152 2109 1697
Fragments arranged by size:
2109 1697 910 881 590 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3720 5119 160
Size: 872 2688 1399 1606
Fragments arranged by size:
2688 1606 1399 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6565
AvaI C'yCGr_G
Cuts at: 1870 1943 3890 1870
Size: 73 1947 4545
Fragments arranged by size:
4545 1947 73
BamHI G'GATC_C
Cuts at: 3757 3895 4262 3757
Size: 138 367 6060
Fragments arranged by size:
6060 367 138
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3681 3886 5341 618
Size: 325 217 627 1894 205 1455 1842
Fragments arranged by size:
1894 1842 1455 627 325 217 205
BanII G_rGCy'C
Cuts at: 1391 2742 3657 3884 1391
Size: 1351 915 227 4072
Fragments arranged by size:
4072 1351 915 227
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2619 928
Size: 971 720 4874
Fragments arranged by size:
4874 971 720
BbvCI CC'TCA_GC
Cuts at: 2973 2973
Size: 6565
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3562 3596 4756 4790 1903
Size: 34 1625 34 1160 34 3678
Fragments arranged by size:
3678 1625 1160 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4457 5667 4457
Size: 1210 5355
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6565
BfrBI ATG'CAT
Cuts at: 3319 3319
Size: 6565
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5176 136
Size: 107 122 71 631 605 3504 1525
Fragments arranged by size:
3504 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6560 6560
Size: 6565
BlpI GC'TnA_GC
Cuts at: 3982 3982
Size: 6565
Bme1580I G_kGCm'C
Cuts at: 1790 2448 4626 5782 1790
Size: 658 2178 1156 2573
Fragments arranged by size:
2573 2178 1156 658
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6565
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2926 4296 5254
Size: 416 53 83 399 24 1509 1370 958
Cuts at: 5254 442
Size: 1753
Fragments arranged by size:
1753 1509 1370 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6565
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3203 3203
Size: 6565
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2981 3109 3173 3671 3686 5225 1239
Size: 1742 128 64 498 15 1539 2579
Fragments arranged by size:
2579 1742 1539 498 128 64 15
Bpu10I CC'TnA_GC
Cuts at: 2973 2973
Size: 6565
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4690 5555 5853 4690
Size: 865 298 5402
Fragments arranged by size:
5402 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5228 882
Size: 617 3729 2219
Fragments arranged by size:
3729 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3461 4356 493
Size: 711 2257 895 2702
Fragments arranged by size:
2702 2257 895 711
BsaBI GATnn'nnATC
Cuts at: 3129 6559 3129
Size: 3430 3135
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4372 4754 5389 275
Size: 53 83 186 936 2839 382 635 1451
Fragments arranged by size:
2839 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2817 4998 5396 5670 5817 6228 2817
Size: 2181 398 274 147 411 3154
Fragments arranged by size:
3154 2181 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3516 3546 1178
Size: 30 165 30 2113 30 4197
Fragments arranged by size:
4197 2113 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2023 2045 2534 3162 3396 1186
Size: 837 22 489 628 234 4355
Fragments arranged by size:
4355 837 628 489 234 22
BseYI C'CCAG_C
Cuts at: 2447 5768 6264 2447
Size: 3321 496 2748
Fragments arranged by size:
3321 2748 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2263 1163
Size: 573 527 5465
Fragments arranged by size:
5465 573 527
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4015 4776 5804 1540
Size: 275 186 2014 761 1028 2301
Fragments arranged by size:
2301 2014 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2742 3657 4626 4711 5782 2742
Size: 915 969 85 1071 3525
Fragments arranged by size:
3525 1071 969 915 85
BsmI GAATG_Cn'
Cuts at: 3507 4076 4169 3507
Size: 569 93 5903
Fragments arranged by size:
5903 569 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2448 2742 3657 3884 4626 4711 5782
Size: 399 658 294 915 227 742 85 1071
Cuts at: 5782 1391
Size: 2174
Fragments arranged by size:
2174 1071 915 742 658 399 294 227
85
BspEI T'CCGG_A
Cuts at: 2817 6228 2817
Size: 3411 3154
BspHI T'CATG_A
Cuts at: 4454 6184 4454
Size: 1730 4835
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3920 844
Size: 3076 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4452 1394
Size: 429 2629 3507
Fragments arranged by size:
3507 2629 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5060 5234 65
Size: 1183 3812 174 1396
Fragments arranged by size:
3812 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5209 5396 1700
Size: 110 3399 187 2869
Fragments arranged by size:
3399 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6565
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6565
BssSI C'ACGA_G
Cuts at: 4625 5637 4625
Size: 1012 5553
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6565
BstBI TT'CG_AA
Cuts at: 5369 5369
Size: 6565
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6565
BstYI r'GATC_y
Cuts at: 1972 3124 3757 3895 4262 4647 4664 6116 6202
Size: 1152 633 138 367 385 17 1452 86
Cuts at: 6202 6214 6560 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1152 633 385 367 346 138
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6013
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5826
Fragments arranged by size:
5826 683 56
BtsI GCAGTG_nn'
Cuts at: 2712 4081 4875 4895 2712
Size: 1369 794 20 4382
Fragments arranged by size:
4382 1369 794 20
ClaI AT'CG_AT
Cuts at: 1554 3133 3322 1554
Size: 1579 189 4797
Fragments arranged by size:
4797 1579 189
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2880 2915 529
Size: 35 2316 35 4179
Fragments arranged by size:
4179 2316 35 35
DraI TTT'AAA
Cuts at: 3869 3869
Size: 6565
DraIII CAC_nnn'GTG
Cuts at: 2241 2241
Size: 6565
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5572 809
Size: 507 4256 1802
Fragments arranged by size:
4256 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2784 3495 4012
Size: 54 1425 50 275 18 954 711 517
Cuts at: 4012 4901 6530 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 954 889 711 517 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4012 1537
Size: 275 2200 4090
Fragments arranged by size:
4090 2200 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2169 2451 3210 4495 1353
Size: 816 282 759 1285 3423
Fragments arranged by size:
3423 1285 816 759 282
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 5152 5526 5672 387
Size: 1064 138 453 3110 374 146 1280
Fragments arranged by size:
3110 1280 1064 453 374 146 138
EcoICRI GAG'CTC
Cuts at: 2740 3655 2740
Size: 915 5650
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2193 2207 2387 2784 2981 3105 3109
Size: 393 561 14 180 397 197 124 4
Cuts at: 3109 3173 3380 3671 3686 3697 3776 4628 5225
Size: 64 207 291 15 11 79 852 597
Cuts at: 5225 6012 6280 1239
Size: 787 268 1524
Fragments arranged by size:
1524 852 787 597 561 397 393 291
268 207 197 180 124 79 64 15
14 11 4
EcoRI G'AATT_C
Cuts at: 3874 4252 3874
Size: 378 6187
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3073 3073
Size: 6565
FspI TGC'GCA
Cuts at: 2315 5071 2315
Size: 2756 3809
HaeII r_GCGC'y
Cuts at: 1678 2828 3405 5712 1678
Size: 1150 577 2307 2531
Fragments arranged by size:
2531 2307 1150 577
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2423 2455 3516 3548 5206
Size: 32 135 32 851 32 1061 32 1658
Cuts at: 5206 5238 5280 5312 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1658 1061 851 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3909 4155 669
Size: 988 281 1971 246 3079
Fragments arranged by size:
3079 1971 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2587 2952 3117 748
Size: 1839 365 165 4196
Fragments arranged by size:
4196 1839 365 165
HpaI GTT'AAC
Cuts at: 4155 4155
Size: 6565
KpnI G_GTAC'C
Cuts at: 3685 3890 3685
Size: 205 6360
MfeI C'AATT_G
Cuts at: 4164 4164
Size: 6565
MluI A'CGCG_T
Cuts at: 6441 6441
Size: 6565
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2829 5679 5863 1305
Size: 1524 2850 184 2007
Fragments arranged by size:
2850 2007 1524 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2786 10
Size: 54 1425 343 954 3789
Fragments arranged by size:
3789 1425 954 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2321 2792 4523 4882 5041 518
Size: 648 1155 471 1731 359 159 2042
Fragments arranged by size:
2042 1731 1155 648 471 359 159
MspA1I CmG'CkG
Cuts at: 1606 2110 2677 3981 4658 5806 6051 1606
Size: 504 567 1304 677 1148 245 2120
Fragments arranged by size:
2120 1304 1148 677 567 504 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6013
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6565
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6565
NotI GC'GGCC_GC
Cuts at: 4012 4012
Size: 6565
NsiI A_TGCA'T
Cuts at: 3321 3321
Size: 6565
NspI r_CATG'y
Cuts at: 1132 1229 2258 2483 3923 5468 1132
Size: 97 1029 225 1440 1545 2229
Fragments arranged by size:
2229 1545 1440 1029 225 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6565
PciI A'CATG_T
Cuts at: 2254 2479 5464 2254
Size: 225 2985 3355
Fragments arranged by size:
3355 2985 225
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6565
PfoI T'CCnGG_A
Cuts at: 2154 2154
Size: 6565
PmeI GTTT'AAAC
Cuts at: 3869 3869
Size: 6565
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4634 4666 6147 6179 4634
Size: 32 1481 32 5020
Fragments arranged by size:
5020 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6565
PsiI TTA'TAA
Cuts at: 4135 4135
Size: 6565
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2355 2387 2355
Size: 32 6533
PstI C_TGCA'G
Cuts at: 830 2052 3917 830
Size: 1222 1865 3478
Fragments arranged by size:
3478 1865 1222
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6565
PvuII CAG'CTG
Cuts at: 2677 2677
Size: 6565
SacI G_AGCT'C
Cuts at: 2742 3657 2742
Size: 915 5650
SalI G'TCGA_C
Cuts at: 1936 3907 1936
Size: 1971 4594
SapI GCTCTTCn'nnn_
Cuts at: 2451 2451
Size: 6565
SbfI CC_TGCA'GG
Cuts at: 3917 3917
Size: 6565
ScaI AGT'ACT
Cuts at: 2915 4813 6525 2915
Size: 1898 1712 2955
Fragments arranged by size:
2955 1898 1712
SfcI C'TryA_G
Cuts at: 826 1045 2048 3913 5048 5729 5920 826
Size: 219 1003 1865 1135 681 191 1471
Fragments arranged by size:
1865 1471 1135 1003 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6565
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6565
SmaI CCC'GGG
Cuts at: 1872 3892 1872
Size: 2020 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4669 5570 5832 820
Size: 197 926 2726 901 262 1553
Fragments arranged by size:
2726 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4356 493
Size: 3863 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6565
SphI G_CATG'C
Cuts at: 1132 3923 1132
Size: 2791 3774
SspI AAT'ATT
Cuts at: 5 52 2647 4489 5
Size: 47 2595 1842 2081
Fragments arranged by size:
2595 2081 1842 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6565
StyI C'CwwG_G
Cuts at: 513 1065 2811 4004 513
Size: 552 1746 1193 3074
Fragments arranged by size:
3074 1746 1193 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2333 4592 4609 4762 4947 5392 325
Size: 1255 753 2259 17 153 185 445 1498
Fragments arranged by size:
2259 1498 1255 753 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2913 4811 6523
Size: 274 80 33 51 1443 936 1898 1712
Cuts at: 6523 96
Size: 138
Fragments arranged by size:
1898 1712 1443 936 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6525 6557 6525
Size: 32 6533
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6565
XbaI T'CTAG_A
Cuts at: 3901 3901
Size: 6565
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6565
XmaI C'CCGG_G
Cuts at: 1870 3890 1870
Size: 2020 4545
XmnI GAAnn'nnTTC
Cuts at: 2468 3118 4694 2468
Size: 650 1576 4339
Fragments arranged by size:
4339 1576 650
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4373 276
Size: 53 83 186 936 2839 2468
Fragments arranged by size:
2839 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspOMI PspXI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI