Restriction map for
pFN21AE4536
(Circular) (Six-base) MAPSORT of: pFN21AE4536.seq Check: 2415 from: 1 to: 6514
pFN21AE4536 6514 bp
With 182 enzymes: SgfI *
March 28, 2012 14:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2356 2429 3557 2356
Size: 73 1128 5313
Fragments arranged by size:
5313 1128 73
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4324 278
Size: 53 83 186 936 2788 2468
Fragments arranged by size:
2788 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2469 3857 1937
Size: 532 1388 4594
Fragments arranged by size:
4594 1388 532
Acc65I G'GTAC_C
Cuts at: 3835 3835
Size: 6514
AclI AA'CG_TT
Cuts at: 1563 4641 5014 1563
Size: 3078 373 3063
Fragments arranged by size:
3078 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2576 2782 3638 4577 5961 6229 1632
Size: 944 206 856 939 1384 268 1917
Fragments arranged by size:
1917 1384 944 939 856 268 206
AfeI AGC'GCT
Cuts at: 3478 3478
Size: 6514
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6317
AflIII A'CryG_T
Cuts at: 1203 2592 5413 6390 1203
Size: 1389 2821 977 1327
Fragments arranged by size:
2821 1389 1327 977
AgeI A'CCGG_T
Cuts at: 5345 5345
Size: 6514
AhdI GACnn_n'nnGTC
Cuts at: 5243 5243
Size: 6514
AleI CACnn'nnGTG
Cuts at: 1166 2036 3080 1166
Size: 870 1044 4600
Fragments arranged by size:
4600 1044 870
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6514
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2474 2506 2474
Size: 32 6482
AlwNI CAG_nnn'CTG
Cuts at: 2191 2362 2554 3706 4257 5829 2191
Size: 171 192 1152 551 1572 2876
Fragments arranged by size:
2876 1572 1152 551 192 171
ApaI G_GGCC'C
Cuts at: 2808 2872 2808
Size: 64 6450
ApaLI G'TGCA_C
Cuts at: 2596 3142 3437 4571 5727 2596
Size: 546 295 1134 1156 3383
Fragments arranged by size:
3383 1156 1134 546 295
ApoI r'AATT_y
Cuts at: 1493 3823 4049 4201 6310 1493
Size: 2330 226 152 2109 1697
Fragments arranged by size:
2330 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5068 160
Size: 872 4036 1606
Fragments arranged by size:
4036 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6514
AvaI C'yCGr_G
Cuts at: 1870 1943 2518 2774 3839 1870
Size: 73 575 256 1065 4545
Fragments arranged by size:
4545 1065 575 256 73
BamHI G'GATC_C
Cuts at: 2338 2475 3171 3408 3844 4211 2338
Size: 137 696 237 436 367 4641
Fragments arranged by size:
4641 696 436 367 237 137
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2417 2691 2876 3835 5290
Size: 325 217 627 630 274 185 959 1455
Cuts at: 5290 618
Size: 1842
Fragments arranged by size:
1842 1455 959 630 627 325 274 217
185
BanII G_rGCy'C
Cuts at: 1391 2081 2099 2145 2715 2808 2872 3468 3738
Size: 690 18 46 570 93 64 596 270
Cuts at: 3738 3833 1391
Size: 95 4072
Fragments arranged by size:
4072 690 596 570 270 95 93 64
46 18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2752 3381 928
Size: 971 853 629 4061
Fragments arranged by size:
4061 971 853 629
BbvCI CC'TCA_GC
Cuts at: 3667 3667
Size: 6514
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4705 4739 1903
Size: 34 50 34 2684 34 3678
Fragments arranged by size:
3678 2684 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4406 5616 4406
Size: 1210 5304
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6514
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2867 3777 5125
Size: 107 122 71 631 605 1195 910 1348
Cuts at: 5125 136
Size: 1525
Fragments arranged by size:
1525 1348 1195 910 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2257 6509 2257
Size: 4252 2262
BlpI GC'TnA_GC
Cuts at: 3931 3931
Size: 6514
Bme1580I G_kGCm'C
Cuts at: 1790 2600 2808 2872 3146 3441 3752 4575 5731
Size: 810 208 64 274 295 311 823 1156
Cuts at: 5731 1790
Size: 2573
Fragments arranged by size:
2573 1156 823 810 311 295 274 208
64
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6514
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3219 4245 5203
Size: 416 53 83 399 24 1802 1026 958
Cuts at: 5203 442
Size: 1753
Fragments arranged by size:
1802 1753 1026 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6514
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2602 3362 2602
Size: 760 5754
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2159 2329 2501 2522 2920 3434 3482 5174
Size: 920 170 172 21 398 514 48 1692
Cuts at: 5174 1239
Size: 2579
Fragments arranged by size:
2579 1692 920 514 398 172 170 48
21
Bpu10I CC'TnA_GC
Cuts at: 3667 3667
Size: 6514
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2189 2267 4639 5504 5802 2189
Size: 78 2372 865 298 2901
Fragments arranged by size:
2901 2372 865 298 78
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2636 3098 5177 882
Size: 617 1137 462 2079 2219
Fragments arranged by size:
2219 2079 1137 617 462
BsaAI yAC'GTr
Cuts at: 493 1204 2649 2854 3363 3436 4305 493
Size: 711 1445 205 509 73 869 2702
Fragments arranged by size:
2702 1445 869 711 509 205 73
BsaBI GATnn'nnATC
Cuts at: 3176 6508 3176
Size: 3332 3182
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2214 2304 3486 3560
Size: 53 83 186 936 681 90 1182 74
Cuts at: 3560 4321 4703 5338 275
Size: 761 382 635 1451
Fragments arranged by size:
1451 1182 936 761 681 635 382 186
90 83 74 53
BsaWI w'CCGG_w
Cuts at: 2493 2575 4947 5345 5619 5766 6177 2493
Size: 82 2372 398 274 147 411 2830
Fragments arranged by size:
2830 2372 411 398 274 147 82
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2474 2504 2633 2663 3407
Size: 30 165 30 1071 30 129 30 744
Cuts at: 3407 3437 1178
Size: 30 4255
Fragments arranged by size:
4255 1071 744 165 129 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2498 2989 3431 1186
Size: 1312 491 442 4269
Fragments arranged by size:
4269 1312 491 442
BseYI C'CCAG_C
Cuts at: 2343 2633 3061 3147 5717 6213 2343
Size: 290 428 86 2570 496 2644
Fragments arranged by size:
2644 2570 496 428 290 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2390 2567 3115 3343 3529 1163
Size: 573 654 177 548 228 186 4148
Fragments arranged by size:
4148 654 573 548 228 186 177
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2236 2833 3247 3964 4725 5753
Size: 275 186 235 597 414 717 761 1028
Cuts at: 5753 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 717 597 414 275 235
186
BsiHKAI G_wGCw'C
Cuts at: 2145 2271 2600 3146 3348 3441 3468 4575 4660
Size: 126 329 546 202 93 27 1107 85
Cuts at: 4660 5731 2145
Size: 1071 2928
Fragments arranged by size:
2928 1107 1071 546 329 202 126 93
85 27
BsmI GAATG_Cn'
Cuts at: 4025 4118 4025
Size: 93 6421
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2099 2145 2271 2600 2715 2808
Size: 399 291 18 46 126 329 115 93
Cuts at: 2808 2872 3146 3348 3441 3468 3738 3752 3833
Size: 64 274 202 93 27 270 14 81
Cuts at: 3833 4575 4660 5731 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 329 291 274 270
202 126 115 93 93 85 81 64
46 27 18 14
BspEI T'CCGG_A
Cuts at: 6177 6177
Size: 6514
BspHI T'CATG_A
Cuts at: 4403 6133 4403
Size: 1730 4784
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2356 2429 3557 3705 3869 844
Size: 1512 73 1128 148 164 3489
Fragments arranged by size:
3489 1512 1128 164 148 73
BsrBI CCG'CTC
Cuts at: 1394 1823 4401 1394
Size: 429 2578 3507
Fragments arranged by size:
3507 2578 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2737 5009 5183 65
Size: 1183 1489 2272 174 1396
Fragments arranged by size:
2272 1489 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2906 3488 5158 5345 1700
Size: 110 1096 582 1670 187 2869
Fragments arranged by size:
2869 1670 1096 582 187 110
BsrGI T'GTAC_A
Cuts at: 96 2025 3072 3627 96
Size: 1929 1047 555 2983
Fragments arranged by size:
2983 1929 1047 555
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6514
BssSI C'ACGA_G
Cuts at: 2261 4574 5586 2261
Size: 2313 1012 3189
Fragments arranged by size:
3189 2313 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2545 3706 794
Size: 1751 1161 3602
Fragments arranged by size:
3602 1751 1161
BstBI TT'CG_AA
Cuts at: 5318 5318
Size: 6514
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6514
BstXI CCAn_nnnn'nTGG
Cuts at: 2050 2050
Size: 6514
BstYI r'GATC_y
Cuts at: 1972 2257 2328 2338 2475 2628 3171 3300 3408
Size: 285 71 10 137 153 543 129 108
Cuts at: 3408 3844 4211 4596 4613 6065 6151 6163 6509
Size: 436 367 385 17 1452 86 12 346
Cuts at: 6509 1972
Size: 1977
Fragments arranged by size:
1977 1452 543 436 385 367 346 285
153 137 129 108 86 71 17 12
10
Bsu36I CC'TnA_GG
Cuts at: 3001 3555 3001
Size: 554 5960
BtgI C'CryG_G
Cuts at: 513 1065 2236 2601 3098 513
Size: 552 1171 365 497 3929
Fragments arranged by size:
3929 1171 552 497 365
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5775
Fragments arranged by size:
5775 683 56
BtsI GCAGTG_nn'
Cuts at: 2043 2069 2286 3155 3652 4030 4824 4844 2043
Size: 26 217 869 497 378 794 20 3713
Fragments arranged by size:
3713 869 794 497 378 217 26 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6514
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6479
DraI TTT'AAA
Cuts at: 3818 3818
Size: 6514
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2456 5521 809
Size: 507 1140 3065 1802
Fragments arranged by size:
3065 1802 1140 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2064 2859
Size: 54 1425 50 275 18 210 24 795
Cuts at: 2859 3087 3244 3387 3961 4850 6479 8
Size: 228 157 143 574 889 1629 43
Fragments arranged by size:
1629 1425 889 795 574 275 228 210
157 143 54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3244 3961 1537
Size: 275 1432 717 4090
Fragments arranged by size:
4090 1432 717 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3726 4444 1353
Size: 2373 718 3423
Fragments arranged by size:
3423 2373 718
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2936 5101 5475 5621 387
Size: 1064 138 1347 2165 374 146 1280
Fragments arranged by size:
2165 1347 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2143 3466 2143
Size: 1323 5191
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 2329 2501 2522 2576 2782 2920
Size: 393 527 170 172 21 54 206 138
Cuts at: 2920 3434 3482 3638 4577 5174 5961 6229 1239
Size: 514 48 156 939 597 787 268 1524
Fragments arranged by size:
1524 939 787 597 527 514 393 268
206 172 170 156 138 54 48 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2444 3710 2444
Size: 1266 5248
EcoO109I rG'GnC_Cy
Cuts at: 2133 2376 2409 2737 2805 2869 2931 3622 2133
Size: 243 33 328 68 64 62 691 5025
Fragments arranged by size:
5025 691 328 243 68 64 62 33
EcoRI G'AATT_C
Cuts at: 3823 4201 3823
Size: 378 6136
EcoRV GAT'ATC
Cuts at: 2942 2942
Size: 6514
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2428 2927 2428
Size: 499 6015
FspI TGC'GCA
Cuts at: 5020 5020
Size: 6514
HaeII r_GCGC'y
Cuts at: 1678 3040 3480 5661 1678
Size: 1362 440 2181 2531
Fragments arranged by size:
2531 2181 1362 440
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5155 5187 5229 5261 1373
Size: 32 135 32 3583 32 42 32 2626
Fragments arranged by size:
3583 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3430 3858 4104 669
Size: 988 281 1492 428 246 3079
Fragments arranged by size:
3079 1492 988 428 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6514
HpaI GTT'AAC
Cuts at: 4104 4104
Size: 6514
KpnI G_GTAC'C
Cuts at: 3839 3839
Size: 6514
MfeI C'AATT_G
Cuts at: 4113 4113
Size: 6514
MluI A'CGCG_T
Cuts at: 2592 6390 2592
Size: 3798 2716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2256 5628 5812 1305
Size: 951 3372 184 2007
Fragments arranged by size:
3372 2007 951 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2042 3089 3389 10
Size: 54 1425 343 210 1047 300 3135
Fragments arranged by size:
3135 1425 1047 343 300 210 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2036 2060 3080 3647 4472 4831 4990
Size: 648 870 24 1020 567 825 359 159
Cuts at: 4990 518
Size: 2042
Fragments arranged by size:
2042 1020 870 825 648 567 359 159
24
MspA1I CmG'CkG
Cuts at: 1606 2359 2802 3151 3460 3930 4607 5755 6000
Size: 753 443 349 309 470 677 1148 245
Cuts at: 6000 1606
Size: 2120
Fragments arranged by size:
2120 1148 753 677 470 443 349 309
245
NaeI GCC'GGC
Cuts at: 2908 3490 2908
Size: 582 5932
NcoI C'CATG_G
Cuts at: 513 1065 2601 513
Size: 552 1536 4426
Fragments arranged by size:
4426 1536 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6514
NgoMIV G'CCGG_C
Cuts at: 2906 3488 2906
Size: 582 5932
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6514
NotI GC'GGCC_GC
Cuts at: 3961 3961
Size: 6514
NspI r_CATG'y
Cuts at: 1132 1229 3310 3872 5417 1132
Size: 97 2081 562 1545 2229
Fragments arranged by size:
2229 2081 1545 562 97
PasI CC'CwG_GG
Cuts at: 1348 2872 1348
Size: 1524 4990
PciI A'CATG_T
Cuts at: 5413 5413
Size: 6514
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2191 2652 2752 2857 1108
Size: 1083 461 100 105 4765
Fragments arranged by size:
4765 1083 461 105 100
PfoI T'CCnGG_A
Cuts at: 2478 3167 3237 3294 3334 3411 2478
Size: 689 70 57 40 77 5581
Fragments arranged by size:
5581 689 77 70 57 40
PmeI GTTT'AAAC
Cuts at: 3818 3818
Size: 6514
PmlI CAC'GTG
Cuts at: 2854 2854
Size: 6514
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2474 2506 4583 4615 6096 6128 2474
Size: 32 2077 32 1481 32 2860
Fragments arranged by size:
2860 2077 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2133 2376 2931 2133
Size: 243 555 5716
Fragments arranged by size:
5716 555 243
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6514
PsiI TTA'TAA
Cuts at: 4084 4084
Size: 6514
PspOMI G'GGCC_C
Cuts at: 2804 2868 2804
Size: 64 6450
PstI C_TGCA'G
Cuts at: 830 2076 2367 2529 2541 3501 3702 3866 830
Size: 1246 291 162 12 960 201 164 3478
Fragments arranged by size:
3478 1246 960 291 201 164 162 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6514
PvuII CAG'CTG
Cuts at: 2359 3151 3460 2359
Size: 792 309 5413
Fragments arranged by size:
5413 792 309
SacI G_AGCT'C
Cuts at: 2145 3468 2145
Size: 1323 5191
SalI G'TCGA_C
Cuts at: 1936 3856 1936
Size: 1920 4594
SanDI GG'GwC_CC
Cuts at: 2133 2133
Size: 6514
SapI GCTCTTCn'nnn_
Cuts at: 3726 3726
Size: 6514
SbfI CC_TGCA'GG
Cuts at: 3866 3866
Size: 6514
ScaI AGT'ACT
Cuts at: 3506 4762 6474 3506
Size: 1256 1712 3546
Fragments arranged by size:
3546 1712 1256
SfcI C'TryA_G
Cuts at: 826 1045 2072 2363 2525 2537 3280 3497 3698
Size: 219 1027 291 162 12 743 217 201
Cuts at: 3698 3862 4997 5678 5869 826
Size: 164 1135 681 191 1471
Fragments arranged by size:
1471 1135 1027 743 681 291 219 217
201 191 164 162 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 2867 2867
Size: 6514
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6514
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6514
SmaI CCC'GGG
Cuts at: 1872 3841 1872
Size: 1969 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2204 2246 4618 5519 5781 820
Size: 197 926 261 42 2372 901 262 1553
Fragments arranged by size:
2372 1553 926 901 262 261 197 42
SnaBI TAC'GTA
Cuts at: 493 4305 493
Size: 3812 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6514
SphI G_CATG'C
Cuts at: 1132 3872 1132
Size: 2740 3774
SspI AAT'ATT
Cuts at: 5 52 4438 5
Size: 47 4386 2081
Fragments arranged by size:
4386 2081 47
StuI AGG'CCT
Cuts at: 1517 2388 1517
Size: 871 5643
StyI C'CwwG_G
Cuts at: 513 1065 2430 2601 2724 2880 3711 3953 513
Size: 552 1365 171 123 156 831 242 3074
Fragments arranged by size:
3074 1365 831 552 242 171 156 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2180 2291 2864 3161 3316 4541 4558
Size: 1255 600 111 573 297 155 1225 17
Cuts at: 4558 4711 4896 5341 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1225 600 573 445 297 185
155 153 111 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2025 3015 3072
Size: 274 80 33 51 1443 48 990 57
Cuts at: 3072 3504 3627 4760 6472 96
Size: 432 123 1133 1712 138
Fragments arranged by size:
1712 1443 1133 990 432 274 138 123
80 57 51 48 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3407 3439 3571 3603 6474 6506 3407
Size: 32 132 32 2871 32 3415
Fragments arranged by size:
3415 2871 132 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2786 1316
Size: 1470 5044
XbaI T'CTAG_A
Cuts at: 3850 3850
Size: 6514
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6514
XmaI C'CCGG_G
Cuts at: 1870 3839 1870
Size: 1969 4545
XmnI GAAnn'nnTTC
Cuts at: 3506 3599 4643 3506
Size: 93 1044 5377
Fragments arranged by size:
5377 1044 93
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4322 276
Size: 53 83 186 936 2788 2468
Fragments arranged by size:
2788 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I
DraIII FseI FspAI KasI NarI NruI NsiI PacI
PspXI PsrI RsrII SacII SexAI SfoI SrfI SwaI
XcmI