Restriction map for
pFN21AE4535
(Circular) (Six-base) MAPSORT of: pFN21AE4535.seq Check: 5655 from: 1 to: 6511
pFN21AE4535 6511 bp
With 182 enzymes: SgfI *
March 28, 2012 14:10 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4321 278
Size: 53 83 186 936 2785 2468
Fragments arranged by size:
2785 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2206 3854 1937
Size: 269 1648 4594
Fragments arranged by size:
4594 1648 269
Acc65I G'GTAC_C
Cuts at: 3832 3832
Size: 6511
AclI AA'CG_TT
Cuts at: 1563 2816 4638 5011 1563
Size: 1253 1822 373 3063
Fragments arranged by size:
3063 1822 1253 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2109 2597 2679 3082 4574 5958 6226 1632
Size: 477 488 82 403 1492 1384 268 1917
Fragments arranged by size:
1917 1492 1384 488 477 403 268 82
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6314
AflIII A'CryG_T
Cuts at: 1203 2464 5410 6387 1203
Size: 1261 2946 977 1327
Fragments arranged by size:
2946 1327 1261 977
AgeI A'CCGG_T
Cuts at: 5342 5342
Size: 6511
AhdI GACnn_n'nnGTC
Cuts at: 5240 5240
Size: 6511
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6511
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2296 1184
Size: 1112 5399
AlwNI CAG_nnn'CTG
Cuts at: 2233 4254 5826 2233
Size: 2021 1572 2918
Fragments arranged by size:
2918 2021 1572
ApaLI G'TGCA_C
Cuts at: 4568 5724 4568
Size: 1156 5355
ApoI r'AATT_y
Cuts at: 1493 2178 2275 2901 3134 3416 3471 3505 3820
Size: 685 97 626 233 282 55 34 315
Cuts at: 3820 4046 4198 6307 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 685 626 315 282 233 226
152 97 55 34
AseI AT'TA_AT
Cuts at: 160 1032 5065 160
Size: 872 4033 1606
Fragments arranged by size:
4033 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6511
AvaI C'yCGr_G
Cuts at: 1870 1943 2490 3836 1870
Size: 73 547 1346 4545
Fragments arranged by size:
4545 1346 547 73
AvrII C'CTAG_G
Cuts at: 2539 2539
Size: 6511
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3100 3133 3100
Size: 33 6478
BamHI G'GATC_C
Cuts at: 2762 3841 4208 2762
Size: 1079 367 5065
Fragments arranged by size:
5065 1079 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2534 3832 5287 618
Size: 325 217 627 747 1298 1455 1842
Fragments arranged by size:
1842 1455 1298 747 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3830 1391
Size: 2439 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2364 3556 928
Size: 971 465 1192 3883
Fragments arranged by size:
3883 1192 971 465
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4702 4736 1903
Size: 34 50 34 2681 34 3678
Fragments arranged by size:
3678 2681 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4403 5613 4403
Size: 1210 5301
BclI T'GATC_A
Cuts at: 1548 2596 3223 1548
Size: 1048 627 4836
Fragments arranged by size:
4836 1048 627
BfrBI ATG'CAT
Cuts at: 2008 2031 3400 2008
Size: 23 1369 5119
Fragments arranged by size:
5119 1369 23
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5122 136
Size: 107 122 71 631 605 3450 1525
Fragments arranged by size:
3450 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6506 6506
Size: 6511
BlpI GC'TnA_GC
Cuts at: 3928 3928
Size: 6511
Bme1580I G_kGCm'C
Cuts at: 1790 2539 4572 5728 1790
Size: 749 2033 1156 2573
Fragments arranged by size:
2573 2033 1156 749
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6511
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3223 4242 5200
Size: 416 53 83 399 24 1806 1019 958
Cuts at: 5200 442
Size: 1753
Fragments arranged by size:
1806 1753 1019 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6511
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2559 5171 1239
Size: 1320 2612 2579
Fragments arranged by size:
2612 2579 1320
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4636 5501 5799 4636
Size: 865 298 5348
Fragments arranged by size:
5348 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3223 3812 5174 882
Size: 617 1724 589 1362 2219
Fragments arranged by size:
2219 1724 1362 617 589
BsaAI yAC'GTr
Cuts at: 493 1204 3632 4302 493
Size: 711 2428 670 2702
Fragments arranged by size:
2702 2428 711 670
BsaBI GATnn'nnATC
Cuts at: 6505 6505
Size: 6511
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4318 4700 5335 275
Size: 53 83 186 936 2785 382 635 1451
Fragments arranged by size:
2785 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4944 5342 5616 5763 6174 4944
Size: 398 274 147 411 5281
Fragments arranged by size:
5281 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3347 3377 1178
Size: 30 165 30 1944 30 4312
Fragments arranged by size:
4312 1944 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6511
BseYI C'CCAG_C
Cuts at: 5714 6210 5714
Size: 496 6015
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2703 1163
Size: 573 967 4971
Fragments arranged by size:
4971 967 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2967 3961 4722 5750 1540
Size: 275 186 966 994 761 1028 2301
Fragments arranged by size:
2301 1028 994 966 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3091 3637 4572 4657 5728 3091
Size: 546 935 85 1071 3874
Fragments arranged by size:
3874 1071 935 546 85
BsmI GAATG_Cn'
Cuts at: 2263 4022 4115 2263
Size: 1759 93 4659
Fragments arranged by size:
4659 1759 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2539 3091 3637 3830 4572 4657 5728
Size: 399 749 552 546 193 742 85 1071
Cuts at: 5728 1391
Size: 2174
Fragments arranged by size:
2174 1071 749 742 552 546 399 193
85
BspEI T'CCGG_A
Cuts at: 6174 6174
Size: 6511
BspHI T'CATG_A
Cuts at: 4400 6130 4400
Size: 1730 4781
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3866 844
Size: 3022 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4398 1394
Size: 429 2575 3507
Fragments arranged by size:
3507 2575 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5006 5180 65
Size: 1183 3758 174 1396
Fragments arranged by size:
3758 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5155 5342 1700
Size: 110 3345 187 2869
Fragments arranged by size:
3345 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6511
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6511
BssSI C'ACGA_G
Cuts at: 4571 5583 4571
Size: 1012 5499
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3509 794
Size: 2715 3796
BstBI TT'CG_AA
Cuts at: 5315 5315
Size: 6511
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6511
BstYI r'GATC_y
Cuts at: 1972 2762 2890 3482 3841 4208 4593 4610 6062
Size: 790 128 592 359 367 385 17 1452
Cuts at: 6062 6148 6160 6506 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 790 592 385 367 359 346
128 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2568 2568
Size: 6511
BtgI C'CryG_G
Cuts at: 513 1065 2317 2967 3374 513
Size: 552 1252 650 407 3650
Fragments arranged by size:
3650 1252 650 552 407
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3716 487
Size: 683 56 2490 3282
Fragments arranged by size:
3282 2490 683 56
BtsI GCAGTG_nn'
Cuts at: 4027 4821 4841 4027
Size: 794 20 5697
Fragments arranged by size:
5697 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6511
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6476
DraI TTT'AAA
Cuts at: 3025 3150 3815 3025
Size: 125 665 5721
Fragments arranged by size:
5721 665 125
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2242 2944 5518 809
Size: 507 926 702 2574 1802
Fragments arranged by size:
2574 1802 926 702 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3377 3958 4847
Size: 54 1425 50 275 18 1547 581 889
Cuts at: 4847 6476 8
Size: 1629 43
Fragments arranged by size:
1629 1547 1425 889 581 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3958 1537
Size: 275 2146 4090
Fragments arranged by size:
4090 2146 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2616 3439 4441 1353
Size: 1263 823 1002 3423
Fragments arranged by size:
3423 1263 1002 823
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5098 5472 5618 387
Size: 1064 138 3509 374 146 1280
Fragments arranged by size:
3509 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2109 2559 2597 2679 3082 4574 5171
Size: 393 477 450 38 82 403 1492 597
Cuts at: 5171 5958 6226 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1492 787 597 477 450 403 393
268 82 38
EcoRI G'AATT_C
Cuts at: 2178 3820 4198 2178
Size: 1642 378 4491
Fragments arranged by size:
4491 1642 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2921 2921
Size: 6511
FspI TGC'GCA
Cuts at: 5017 5017
Size: 6511
HaeII r_GCGC'y
Cuts at: 1678 5658 1678
Size: 3980 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5152 5184 5226 5258 1373
Size: 32 135 32 3580 32 42 32 2626
Fragments arranged by size:
3580 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3609 3855 4101 669
Size: 988 281 1671 246 246 3079
Fragments arranged by size:
3079 1671 988 281 246 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6511
HpaI GTT'AAC
Cuts at: 4101 4101
Size: 6511
KpnI G_GTAC'C
Cuts at: 3836 3836
Size: 6511
MfeI C'AATT_G
Cuts at: 4110 4110
Size: 6511
MluI A'CGCG_T
Cuts at: 6387 6387
Size: 6511
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2987 5625 5809 1305
Size: 1682 2638 184 2007
Fragments arranged by size:
2638 2007 1682 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3379 10
Size: 54 1425 343 1547 3142
Fragments arranged by size:
3142 1547 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2469 2990 3405 4469 4828 4987 518
Size: 648 1303 521 415 1064 359 159 2042
Fragments arranged by size:
2042 1303 1064 648 521 415 359 159
MspA1I CmG'CkG
Cuts at: 1606 3927 4604 5752 5997 1606
Size: 2321 677 1148 245 2120
Fragments arranged by size:
2321 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2317 513
Size: 552 1252 4707
Fragments arranged by size:
4707 1252 552
NdeI CA'TA_TG
Cuts at: 387 2027 3284 387
Size: 1640 1257 3614
Fragments arranged by size:
3614 1640 1257
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6511
NotI GC'GGCC_GC
Cuts at: 3958 3958
Size: 6511
NsiI A_TGCA'T
Cuts at: 2010 2033 3402 2010
Size: 23 1369 5119
Fragments arranged by size:
5119 1369 23
NspI r_CATG'y
Cuts at: 1132 1229 2068 2468 2995 3869 5414 1132
Size: 97 839 400 527 874 1545 2229
Fragments arranged by size:
2229 1545 874 839 527 400 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6511
PciI A'CATG_T
Cuts at: 2464 5410 2464
Size: 2946 3565
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2201 3040 1108
Size: 1093 839 4579
Fragments arranged by size:
4579 1093 839
PfoI T'CCnGG_A
Cuts at: 2719 3163 2719
Size: 444 6067
PmeI GTTT'AAAC
Cuts at: 3815 3815
Size: 6511
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4580 4612 6093 6125 4580
Size: 32 1481 32 4966
Fragments arranged by size:
4966 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6511
PsiI TTA'TAA
Cuts at: 3276 4081 3276
Size: 805 5706
PstI C_TGCA'G
Cuts at: 830 3863 830
Size: 3033 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6511
SalI G'TCGA_C
Cuts at: 1936 3853 1936
Size: 1917 4594
SapI GCTCTTCn'nnn_
Cuts at: 2616 2616
Size: 6511
SbfI CC_TGCA'GG
Cuts at: 3863 3863
Size: 6511
ScaI AGT'ACT
Cuts at: 4759 6471 4759
Size: 1712 4799
SfcI C'TryA_G
Cuts at: 826 1045 2524 3859 4994 5675 5866 826
Size: 219 1479 1335 1135 681 191 1471
Fragments arranged by size:
1479 1471 1335 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6511
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6511
SmaI CCC'GGG
Cuts at: 1872 3838 1872
Size: 1966 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4615 5516 5778 820
Size: 197 926 2672 901 262 1553
Fragments arranged by size:
2672 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4302 493
Size: 3809 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6511
SphI G_CATG'C
Cuts at: 1132 2068 3869 1132
Size: 936 1801 3774
Fragments arranged by size:
3774 1801 936
SspI AAT'ATT
Cuts at: 5 52 3156 3694 4435 5
Size: 47 3104 538 741 2081
Fragments arranged by size:
3104 2081 741 538 47
StuI AGG'CCT
Cuts at: 1517 3251 1517
Size: 1734 4777
StyI C'CwwG_G
Cuts at: 513 1065 2317 2539 2777 3252 3294 3950 513
Size: 552 1252 222 238 475 42 656 3074
Fragments arranged by size:
3074 1252 656 552 475 238 222 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2522 3035 3245 4538 4555 4708 4893
Size: 1255 942 513 210 1293 17 153 185
Cuts at: 4893 5338 325
Size: 445 1498
Fragments arranged by size:
1498 1293 1255 942 513 445 210 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4757 6469 96
Size: 274 80 33 51 1443 2780 1712 138
Fragments arranged by size:
2780 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2416 2448 3347 3379 6471 6503 2416
Size: 32 899 32 3092 32 2424
Fragments arranged by size:
3092 2424 899 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6511
XbaI T'CTAG_A
Cuts at: 2846 3847 2846
Size: 1001 5510
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6511
XmaI C'CCGG_G
Cuts at: 1870 3836 1870
Size: 1966 4545
XmnI GAAnn'nnTTC
Cuts at: 2728 2904 4640 2728
Size: 176 1736 4599
Fragments arranged by size:
4599 1736 176
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4319 276
Size: 53 83 186 936 2785 2468
Fragments arranged by size:
2785 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI