Restriction map for
pFN21AE4533
(Circular) (Six-base) MAPSORT of: pFN21AE4533.seq Check: 8389 from: 1 to: 6499
pFN21AE4533 6499 bp
With 182 enzymes: SgfI *
March 28, 2012 14:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2286 2286
Size: 6499
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3414 4309 278
Size: 53 83 186 936 1878 895 2468
Fragments arranged by size:
2468 1878 936 895 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3842 1937
Size: 1905 4594
Acc65I G'GTAC_C
Cuts at: 3820 3820
Size: 6499
AclI AA'CG_TT
Cuts at: 1563 4626 4999 1563
Size: 3063 373 3063
Fragments arranged by size:
3063 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2129 2778 4562 5946 6214 1632
Size: 497 649 1784 1384 268 1917
Fragments arranged by size:
1917 1784 1384 649 497 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6302
AflIII A'CryG_T
Cuts at: 1203 5398 6375 1203
Size: 4195 977 1327
Fragments arranged by size:
4195 1327 977
AgeI A'CCGG_T
Cuts at: 5330 5330
Size: 6499
AhdI GACnn_n'nnGTC
Cuts at: 5228 5228
Size: 6499
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6499
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3714 1184
Size: 2530 3969
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3440 3472 3440
Size: 32 6467
AlwNI CAG_nnn'CTG
Cuts at: 2104 4242 5814 2104
Size: 2138 1572 2789
Fragments arranged by size:
2789 2138 1572
ApaI G_GGCC'C
Cuts at: 3162 3162
Size: 6499
ApaLI G'TGCA_C
Cuts at: 4556 5712 4556
Size: 1156 5343
ApoI r'AATT_y
Cuts at: 1493 2250 2887 3808 4034 4186 6295 1493
Size: 757 637 921 226 152 2109 1697
Fragments arranged by size:
2109 1697 921 757 637 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5053 160
Size: 872 4021 1606
Fragments arranged by size:
4021 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6499
AvaI C'yCGr_G
Cuts at: 1870 1943 2176 2529 2676 3824 1870
Size: 73 233 353 147 1148 4545
Fragments arranged by size:
4545 1148 353 233 147 73
BamHI G'GATC_C
Cuts at: 2592 3829 4196 2592
Size: 1237 367 4895
Fragments arranged by size:
4895 1237 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2618 3320 3820 5275 618
Size: 325 217 627 831 702 500 1455 1842
Fragments arranged by size:
1842 1455 831 702 627 500 325 217
BanII G_rGCy'C
Cuts at: 1391 2036 2486 2509 3162 3172 3818 1391
Size: 645 450 23 653 10 646 4072
Fragments arranged by size:
4072 653 646 645 450 23 10
BbeI G_GCGC'C
Cuts at: 3324 3324
Size: 6499
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2869 928
Size: 971 970 4558
Fragments arranged by size:
4558 971 970
BbvCI CC'TCA_GC
Cuts at: 2499 3104 2499
Size: 605 5894
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4690 4724 1903
Size: 34 2753 34 3678
Fragments arranged by size:
3678 2753 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3206 4391 5601 3206
Size: 1185 1210 4104
Fragments arranged by size:
4104 1210 1185
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6499
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5110 136
Size: 107 122 71 631 605 3438 1525
Fragments arranged by size:
3438 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6494 6494
Size: 6499
BlpI GC'TnA_GC
Cuts at: 2420 3916 2420
Size: 1496 5003
Bme1580I G_kGCm'C
Cuts at: 1790 2558 2990 3162 3346 3552 3693 4560 5716
Size: 768 432 172 184 206 141 867 1156
Cuts at: 5716 1790
Size: 2573
Fragments arranged by size:
2573 1156 867 768 432 206 184 172
141
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6499
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2158 2602 2665
Size: 416 53 83 399 24 741 444 63
Cuts at: 2665 3448 4230 5188 442
Size: 783 782 958 1753
Fragments arranged by size:
1753 958 783 782 741 444 416 399
83 63 53 24
BmtI G_CTAG'C
Cuts at: 1056 3705 1056
Size: 2649 3850
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2191 2461 2500 2191
Size: 270 39 6190
Fragments arranged by size:
6190 270 39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2727 3205 5159 1239
Size: 1488 478 1954 2579
Fragments arranged by size:
2579 1954 1488 478
Bpu10I CC'TnA_GC
Cuts at: 2030 2237 2499 3104 2030
Size: 207 262 605 5425
Fragments arranged by size:
5425 605 262 207
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4624 5489 5787 4624
Size: 865 298 5336
Fragments arranged by size:
5336 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2162 2798 5162 882
Size: 617 663 636 2364 2219
Fragments arranged by size:
2364 2219 663 636 617
BsaAI yAC'GTr
Cuts at: 493 1204 4290 493
Size: 711 3086 2702
Fragments arranged by size:
3086 2702 711
BsaBI GATnn'nnATC
Cuts at: 2927 3428 6493 2927
Size: 501 3065 2933
Fragments arranged by size:
3065 2933 501
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3321 3411 4306 4688
Size: 53 83 186 936 1788 90 895 382
Cuts at: 4688 5323 275
Size: 635 1451
Fragments arranged by size:
1788 1451 936 895 635 382 186 90
83 53
BsaWI w'CCGG_w
Cuts at: 3209 3445 4932 5330 5604 5751 6162 3209
Size: 236 1487 398 274 147 411 3546
Fragments arranged by size:
3546 1487 411 398 274 236 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6274
Fragments arranged by size:
6274 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2262 1186
Size: 1076 5423
BseYI C'CCAG_C
Cuts at: 2508 5702 6198 2508
Size: 3194 496 2809
Fragments arranged by size:
3194 2809 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3642 3696 1163
Size: 573 1906 54 3966
Fragments arranged by size:
3966 1906 573 54
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3949 4710 5738 1540
Size: 275 186 1948 761 1028 2301
Fragments arranged by size:
2301 1948 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2486 3172 4560 4645 5716 2486
Size: 686 1388 85 1071 3269
Fragments arranged by size:
3269 1388 1071 686 85
BsmI GAATG_Cn'
Cuts at: 4010 4103 4010
Size: 93 6406
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2486 2509 2558 2990 3162 3172
Size: 399 246 450 23 49 432 172 10
Cuts at: 3172 3346 3552 3693 3818 4560 4645 5716 1391
Size: 174 206 141 125 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 450 432 399 246 206
174 172 141 125 85 49 23 10
BspEI T'CCGG_A
Cuts at: 3209 6162 3209
Size: 2953 3546
BspHI T'CATG_A
Cuts at: 4388 6118 4388
Size: 1730 4769
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2286 3854 844
Size: 1442 1568 3489
Fragments arranged by size:
3489 1568 1442
BsrBI CCG'CTC
Cuts at: 1394 1823 4386 1394
Size: 429 2563 3507
Fragments arranged by size:
3507 2563 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4994 5168 65
Size: 1183 3746 174 1396
Fragments arranged by size:
3746 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5143 5330 1700
Size: 110 3333 187 2869
Fragments arranged by size:
3333 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3736 96
Size: 3640 2859
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6499
BssSI C'ACGA_G
Cuts at: 3639 4559 5571 3639
Size: 920 1012 4567
Fragments arranged by size:
4567 1012 920
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6499
BstBI TT'CG_AA
Cuts at: 2260 5303 2260
Size: 3043 3456
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6499
BstXI CCAn_nnnn'nTGG
Cuts at: 2549 2615 2549
Size: 66 6433
BstYI r'GATC_y
Cuts at: 1972 2592 3474 3829 4196 4581 4598 6050 6136
Size: 620 882 355 367 385 17 1452 86
Cuts at: 6136 6148 6494 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 882 620 385 367 355 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3771 3771
Size: 6499
BtgI C'CryG_G
Cuts at: 513 1065 2409 3163 513
Size: 552 1344 754 3849
Fragments arranged by size:
3849 1344 754 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5760
Fragments arranged by size:
5760 683 56
BtsI GCAGTG_nn'
Cuts at: 2288 4015 4809 4829 2288
Size: 1727 794 20 3958
Fragments arranged by size:
3958 1727 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6499
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2593 2628 529
Size: 35 2029 35 4400
Fragments arranged by size:
4400 2029 35 35
DraI TTT'AAA
Cuts at: 3803 3803
Size: 6499
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5506 809
Size: 507 4190 1802
Fragments arranged by size:
4190 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3565 3946 4835
Size: 54 1425 50 275 18 1735 381 889
Cuts at: 4835 6464 8
Size: 1629 43
Fragments arranged by size:
1735 1629 1425 889 381 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3946 1537
Size: 275 2134 4090
Fragments arranged by size:
4090 2134 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2469 2530 2814 3302 3559 4429 1353
Size: 1116 61 284 488 257 870 3423
Fragments arranged by size:
3423 1116 870 488 284 257 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5086 5460 5606 387
Size: 1064 138 3497 374 146 1280
Fragments arranged by size:
3497 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2484 3170 2484
Size: 686 5813
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2727 2778 3205 4562 5159 5946
Size: 393 497 598 51 427 1357 597 787
Cuts at: 5946 6214 1239
Size: 268 1524
Fragments arranged by size:
1524 1357 787 598 597 497 427 393
268 51
EcoNI CCTnn'n_nnAGG
Cuts at: 3399 3399
Size: 6499
EcoO109I rG'GnC_Cy
Cuts at: 2101 3158 3159 2101
Size: 1057 1 5441
Fragments arranged by size:
5441 1057 1
EcoRI G'AATT_C
Cuts at: 3808 4186 3808
Size: 378 6121
EcoRV GAT'ATC
Cuts at: 2686 2686
Size: 6499
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2656 2656
Size: 6499
FspI TGC'GCA
Cuts at: 5005 5005
Size: 6499
HaeII r_GCGC'y
Cuts at: 1678 3324 5646 1678
Size: 1646 2322 2531
Fragments arranged by size:
2531 2322 1646
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3277 3309 3366 3398 5140
Size: 32 135 32 1705 32 57 32 1742
Cuts at: 5140 5172 5214 5246 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1742 1705 135 57 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2344 2371 3070 3843 4089 669
Size: 988 281 406 27 699 773 246 3079
Fragments arranged by size:
3079 988 773 699 406 281 246 27
HindIII A'AGCT_T
Cuts at: 748 2012 748
Size: 1264 5235
HpaI GTT'AAC
Cuts at: 3070 4089 3070
Size: 1019 5480
KasI G'GCGC_C
Cuts at: 3320 3320
Size: 6499
KpnI G_GTAC'C
Cuts at: 3824 3824
Size: 6499
MfeI C'AATT_G
Cuts at: 4098 4098
Size: 6499
MluI A'CGCG_T
Cuts at: 6375 6375
Size: 6499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2274 2839 5613 5797 1305
Size: 969 565 2774 184 2007
Fragments arranged by size:
2774 2007 969 565 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4677
Fragments arranged by size:
4677 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2978 4457 4816 4975 518
Size: 648 1812 1479 359 159 2042
Fragments arranged by size:
2042 1812 1479 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2587 3165 3179 3358 3563 3915 4592 5740
Size: 981 578 14 179 205 352 677 1148
Cuts at: 5740 5985 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 981 677 578 352 245 205
179 14
NarI GG'CG_CC
Cuts at: 3321 3321
Size: 6499
NcoI C'CATG_G
Cuts at: 513 1065 2409 513
Size: 552 1344 4603
Fragments arranged by size:
4603 1344 552
NdeI CA'TA_TG
Cuts at: 387 2380 387
Size: 1993 4506
NheI G'CTAG_C
Cuts at: 1052 3701 1052
Size: 2649 3850
NotI GC'GGCC_GC
Cuts at: 3946 3946
Size: 6499
NspI r_CATG'y
Cuts at: 1132 1229 2766 3857 5402 1132
Size: 97 1537 1091 1545 2229
Fragments arranged by size:
2229 1545 1537 1091 97
PasI CC'CwG_GG
Cuts at: 1348 2549 3092 3466 1348
Size: 1201 543 374 4381
Fragments arranged by size:
4381 1201 543 374
PciI A'CATG_T
Cuts at: 5398 5398
Size: 6499
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3316 1108
Size: 2208 4291
PfoI T'CCnGG_A
Cuts at: 2466 2466
Size: 6499
PmeI GTTT'AAAC
Cuts at: 3803 3803
Size: 6499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4568 4600 6081 6113 4568
Size: 32 1481 32 4954
Fragments arranged by size:
4954 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2101 2101
Size: 6499
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6499
PsiI TTA'TAA
Cuts at: 4069 4069
Size: 6499
PspOMI G'GGCC_C
Cuts at: 3158 3158
Size: 6499
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2000 2032 2000
Size: 32 6467
PstI C_TGCA'G
Cuts at: 830 2283 2716 3609 3851 830
Size: 1453 433 893 242 3478
Fragments arranged by size:
3478 1453 893 433 242
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6499
PvuII CAG'CTG
Cuts at: 3358 3563 3358
Size: 205 6294
SacI G_AGCT'C
Cuts at: 2486 3172 2486
Size: 686 5813
SacII CC_GC'GG
Cuts at: 3166 3166
Size: 6499
SalI G'TCGA_C
Cuts at: 1936 3841 1936
Size: 1905 4594
SbfI CC_TGCA'GG
Cuts at: 3851 3851
Size: 6499
ScaI AGT'ACT
Cuts at: 4747 6459 4747
Size: 1712 4787
SfcI C'TryA_G
Cuts at: 826 1045 2279 2712 3605 3847 4982 5663 5854
Size: 219 1234 433 893 242 1135 681 191
Cuts at: 5854 826
Size: 1471
Fragments arranged by size:
1471 1234 1135 893 681 433 242 219
191
SfoI GGC'GCC
Cuts at: 3322 3322
Size: 6499
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6499
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6499
SmaI CCC'GGG
Cuts at: 1872 2531 2678 3826 1872
Size: 659 147 1148 4545
Fragments arranged by size:
4545 1148 659 147
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4603 5504 5766 820
Size: 197 926 2660 901 262 1553
Fragments arranged by size:
2660 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4290 493
Size: 3797 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6499
SphI G_CATG'C
Cuts at: 1132 2766 3857 1132
Size: 1634 1091 3774
Fragments arranged by size:
3774 1634 1091
SspI AAT'ATT
Cuts at: 5 52 4423 5
Size: 47 4371 2081
Fragments arranged by size:
4371 2081 47
StuI AGG'CCT
Cuts at: 1517 2999 3044 1517
Size: 1482 45 4972
Fragments arranged by size:
4972 1482 45
StyI C'CwwG_G
Cuts at: 513 1065 2409 3938 513
Size: 552 1344 1529 3074
Fragments arranged by size:
3074 1529 1344 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2621 4526 4543 4696 4881 5326 325
Size: 1255 1041 1905 17 153 185 445 1498
Fragments arranged by size:
1905 1498 1255 1041 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3736 4745 6457
Size: 274 80 33 51 1443 1759 1009 1712
Cuts at: 6457 96
Size: 138
Fragments arranged by size:
1759 1712 1443 1009 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3123 3155 6459 6491 3123
Size: 32 3304 32 3131
Fragments arranged by size:
3304 3131 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2671 1316
Size: 1355 5144
XbaI T'CTAG_A
Cuts at: 3835 3835
Size: 6499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3242 3242
Size: 6499
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6499
XmaI C'CCGG_G
Cuts at: 1870 2529 2676 3824 1870
Size: 659 147 1148 4545
Fragments arranged by size:
4545 1148 659 147
XmnI GAAnn'nnTTC
Cuts at: 2823 3296 4628 2823
Size: 473 1332 4694
Fragments arranged by size:
4694 1332 473
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3412 4307 276
Size: 53 83 186 936 1878 895 2468
Fragments arranged by size:
2468 1878 936 895 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I
DraIII FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspXI RsrII SanDI SapI SexAI SfiI SrfI
SwaI