Restriction map for
pFN21AE4532
(Circular) (Six-base) MAPSORT of: pFN21AE4532.seq Check: 3623 from: 1 to: 6487
pFN21AE4532 6487 bp
With 182 enzymes: SgfI *
March 28, 2012 14:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3054 3054
Size: 6487
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4297 278
Size: 53 83 186 936 2761 2468
Fragments arranged by size:
2761 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2793 3090 3830 1937
Size: 856 297 740 4594
Fragments arranged by size:
4594 856 740 297
Acc65I G'GTAC_C
Cuts at: 3808 3808
Size: 6487
AclI AA'CG_TT
Cuts at: 1563 4614 4987 1563
Size: 3051 373 3063
Fragments arranged by size:
3063 3051 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2573 3017 3517 3770 4550 5934 6202 1632
Size: 941 444 500 253 780 1384 268 1917
Fragments arranged by size:
1917 1384 941 780 500 444 268 253
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6290
AflIII A'CryG_T
Cuts at: 1203 2311 5386 6363 1203
Size: 1108 3075 977 1327
Fragments arranged by size:
3075 1327 1108 977
AgeI A'CCGG_T
Cuts at: 5318 5318
Size: 6487
AhdI GACnn_n'nnGTC
Cuts at: 5216 5216
Size: 6487
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6487
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2138 1184
Size: 954 5533
AlwNI CAG_nnn'CTG
Cuts at: 2695 2995 3118 3137 3736 4230 5802 2695
Size: 300 123 19 599 494 1572 3380
Fragments arranged by size:
3380 1572 599 494 300 123 19
ApaI G_GGCC'C
Cuts at: 2672 2672
Size: 6487
ApaLI G'TGCA_C
Cuts at: 4544 5700 4544
Size: 1156 5331
ApoI r'AATT_y
Cuts at: 1493 3796 4022 4174 6283 1493
Size: 2303 226 152 2109 1697
Fragments arranged by size:
2303 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5041 160
Size: 872 4009 1606
Fragments arranged by size:
4009 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6487
AvaI C'yCGr_G
Cuts at: 1870 1943 2367 3812 1870
Size: 73 424 1445 4545
Fragments arranged by size:
4545 1445 424 73
BamHI G'GATC_C
Cuts at: 3707 3817 4184 3707
Size: 110 367 6010
Fragments arranged by size:
6010 367 110
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3083 3808 5263 618
Size: 325 217 627 1296 725 1455 1842
Fragments arranged by size:
1842 1455 1296 725 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2672 3027 3806 1391
Size: 1281 355 779 4072
Fragments arranged by size:
4072 1281 779 355
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2308 2972 928
Size: 971 409 664 4443
Fragments arranged by size:
4443 971 664 409
BbvCI CC'TCA_GC
Cuts at: 3057 3138 3057
Size: 81 6406
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4678 4712 1903
Size: 34 2741 34 3678
Fragments arranged by size:
3678 2741 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4379 5589 4379
Size: 1210 5277
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6487
BfrBI ATG'CAT
Cuts at: 2050 2050
Size: 6487
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2606 5098 136
Size: 107 122 71 631 605 934 2492 1525
Fragments arranged by size:
2492 1525 934 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6482 6482
Size: 6487
BlpI GC'TnA_GC
Cuts at: 2610 3026 3904 2610
Size: 416 878 5193
Fragments arranged by size:
5193 878 416
Bme1580I G_kGCm'C
Cuts at: 1790 2672 3131 3351 3567 4548 5704 1790
Size: 882 459 220 216 981 1156 2573
Fragments arranged by size:
2573 1156 981 882 459 220 216
BmgBI CAC'GTC
Cuts at: 1318 2314 1318
Size: 996 5491
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4218 5176 442
Size: 416 53 83 399 24 2801 958 1753
Fragments arranged by size:
2801 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6487
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2222 2222
Size: 6487
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2237 2531 2979 5147 1239
Size: 998 294 448 2168 2579
Fragments arranged by size:
2579 2168 998 448 294
Bpu10I CC'TnA_GC
Cuts at: 3057 3138 3057
Size: 81 6406
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4612 5477 5775 4612
Size: 865 298 5324
Fragments arranged by size:
5324 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2798 2925 5150 882
Size: 617 1299 127 2225 2219
Fragments arranged by size:
2225 2219 1299 617 127
BsaAI yAC'GTr
Cuts at: 493 1204 4278 493
Size: 711 3074 2702
Fragments arranged by size:
3074 2702 711
BsaBI GATnn'nnATC
Cuts at: 6481 6481
Size: 6487
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4294 4676 5311 275
Size: 53 83 186 936 2761 382 635 1451
Fragments arranged by size:
2761 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4920 5318 5592 5739 6150 4920
Size: 398 274 147 411 5257
Fragments arranged by size:
5257 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2210 2240 2708 2738 1178
Size: 30 165 30 807 30 468 30 4927
Fragments arranged by size:
4927 807 468 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6487
BseYI C'CCAG_C
Cuts at: 2210 2688 2720 5690 6186 2210
Size: 478 32 2970 496 2511
Fragments arranged by size:
2970 2511 496 478 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2292 3032 1163
Size: 573 556 740 4618
Fragments arranged by size:
4618 740 573 556
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3937 4698 5726 1540
Size: 275 186 1936 761 1028 2301
Fragments arranged by size:
2301 1936 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2736 2844 3027 4548 4633 5704 2736
Size: 108 183 1521 85 1071 3519
Fragments arranged by size:
3519 1521 1071 183 108 85
BsmI GAATG_Cn'
Cuts at: 3998 4091 3998
Size: 93 6394
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2672 2736 2844 3027 3131 3351 3567
Size: 399 882 64 108 183 104 220 216
Cuts at: 3567 3806 4548 4633 5704 1391
Size: 239 742 85 1071 2174
Fragments arranged by size:
2174 1071 882 742 399 239 220 216
183 108 104 85 64
BspEI T'CCGG_A
Cuts at: 6150 6150
Size: 6487
BspHI T'CATG_A
Cuts at: 2154 4376 6106 2154
Size: 2222 1730 2535
Fragments arranged by size:
2535 2222 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3054 3842 844
Size: 2210 788 3489
Fragments arranged by size:
3489 2210 788
BsrBI CCG'CTC
Cuts at: 1394 1823 2206 3394 4374 1394
Size: 429 383 1188 980 3507
Fragments arranged by size:
3507 1188 980 429 383
BsrDI GCAATG_nn'
Cuts at: 65 1248 4982 5156 65
Size: 1183 3734 174 1396
Fragments arranged by size:
3734 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5131 5318 1700
Size: 110 3321 187 2869
Fragments arranged by size:
3321 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6487
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6487
BssSI C'ACGA_G
Cuts at: 4547 5559 4547
Size: 1012 5475
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6487
BstBI TT'CG_AA
Cuts at: 5291 5291
Size: 6487
BstEII G'GTnAC_C
Cuts at: 1184 3670 1184
Size: 2486 4001
BstXI CCAn_nnnn'nTGG
Cuts at: 3187 3187
Size: 6487
BstYI r'GATC_y
Cuts at: 1972 3707 3817 4184 4569 4586 6038 6124 6136
Size: 1735 110 367 385 17 1452 86 12
Cuts at: 6136 6482 1972
Size: 346 1977
Fragments arranged by size:
1977 1735 1452 385 367 346 110 86
17 12
BstZ17I GTA'TAC
Cuts at: 2794 2794
Size: 6487
BtgI C'CryG_G
Cuts at: 513 1065 2004 3123 513
Size: 552 939 1119 3877
Fragments arranged by size:
3877 1119 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2875 487
Size: 683 56 1649 4099
Fragments arranged by size:
4099 1649 683 56
BtsI GCAGTG_nn'
Cuts at: 2873 3607 3790 4003 4797 4817 2873
Size: 734 183 213 794 20 4543
Fragments arranged by size:
4543 794 734 213 183 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6487
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6452
DraI TTT'AAA
Cuts at: 3791 3791
Size: 6487
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5494 809
Size: 507 4178 1802
Fragments arranged by size:
4178 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3120 3934 4823
Size: 54 1425 50 275 18 1290 814 889
Cuts at: 4823 6452 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1290 889 814 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3934 1537
Size: 275 2122 4090
Fragments arranged by size:
4090 2122 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4417 1353
Size: 3064 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5074 5448 5594 387
Size: 1064 138 3485 374 146 1280
Fragments arranged by size:
3485 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3025 3025
Size: 6487
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 2531 2573 2979 3017 3517 3770
Size: 393 605 294 42 406 38 500 253
Cuts at: 3770 4550 5147 5934 6202 1239
Size: 780 597 787 268 1524
Fragments arranged by size:
1524 787 780 605 597 500 406 393
294 268 253 42 38
EcoNI CCTnn'n_nnAGG
Cuts at: 2849 3056 2849
Size: 207 6280
EcoO109I rG'GnC_Cy
Cuts at: 2281 2668 2980 3115 3157 3583 2281
Size: 387 312 135 42 426 5185
Fragments arranged by size:
5185 426 387 312 135 42
EcoRI G'AATT_C
Cuts at: 3796 4174 3796
Size: 378 6109
EcoRV GAT'ATC
Cuts at: 2185 2185
Size: 6487
FspI TGC'GCA
Cuts at: 4993 4993
Size: 6487
HaeII r_GCGC'y
Cuts at: 1678 2518 3555 5634 1678
Size: 840 1037 2079 2531
Fragments arranged by size:
2531 2079 1037 840
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2485 2517 2689 2708 2721
Size: 32 135 32 913 32 172 19 13
Cuts at: 2721 2740 2752 2784 3135 3159 3167 3191 5128
Size: 19 12 32 351 24 8 24 1937
Cuts at: 5128 5160 5202 5234 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1937 913 351 172 135 42 32
32 32 32 32 32 24 24 19
19 13 12 8
HincII GTy'rAC
Cuts at: 669 1657 1938 3831 4077 669
Size: 988 281 1893 246 3079
Fragments arranged by size:
3079 1893 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2467 748
Size: 1719 4768
HpaI GTT'AAC
Cuts at: 4077 4077
Size: 6487
KpnI G_GTAC'C
Cuts at: 3812 3812
Size: 6487
MfeI C'AATT_G
Cuts at: 4086 4086
Size: 6487
MluI A'CGCG_T
Cuts at: 6363 6363
Size: 6487
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2014 2678 5601 5785 1305
Size: 709 664 2923 184 2007
Fragments arranged by size:
2923 2007 709 664 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3122 10
Size: 54 1425 343 1290 3375
Fragments arranged by size:
3375 1425 1290 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2120 3329 4445 4804 4963 518
Size: 648 954 1209 1116 359 159 2042
Fragments arranged by size:
2042 1209 1116 954 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2811 3903 4580 5728 5973 1606
Size: 1205 1092 677 1148 245 2120
Fragments arranged by size:
2120 1205 1148 1092 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3123 513
Size: 552 939 1119 3877
Fragments arranged by size:
3877 1119 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6487
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6487
NotI GC'GGCC_GC
Cuts at: 3934 3934
Size: 6487
NruI TCG'CGA
Cuts at: 2534 2534
Size: 6487
NsiI A_TGCA'T
Cuts at: 2052 2052
Size: 6487
NspI r_CATG'y
Cuts at: 1132 1229 3845 5390 1132
Size: 97 2616 1545 2229
Fragments arranged by size:
2616 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6487
PciI A'CATG_T
Cuts at: 5386 5386
Size: 6487
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6487
PfoI T'CCnGG_A
Cuts at: 2619 2700 2844 2619
Size: 81 144 6262
Fragments arranged by size:
6262 144 81
PmeI GTTT'AAAC
Cuts at: 3791 3791
Size: 6487
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4556 4588 6069 6101 4556
Size: 32 1481 32 4942
Fragments arranged by size:
4942 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2281 2980 3115 3157 2281
Size: 699 135 42 5611
Fragments arranged by size:
5611 699 135 42
PshAI GACnn'nnGTC
Cuts at: 1613 2331 1613
Size: 718 5769
PsiI TTA'TAA
Cuts at: 4057 4057
Size: 6487
PspOMI G'GGCC_C
Cuts at: 2668 2668
Size: 6487
PstI C_TGCA'G
Cuts at: 830 3839 830
Size: 3009 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6487
SacI G_AGCT'C
Cuts at: 3027 3027
Size: 6487
SalI G'TCGA_C
Cuts at: 1936 3829 1936
Size: 1893 4594
SbfI CC_TGCA'GG
Cuts at: 3839 3839
Size: 6487
ScaI AGT'ACT
Cuts at: 3035 4735 6447 3035
Size: 1700 1712 3075
Fragments arranged by size:
3075 1712 1700
SfcI C'TryA_G
Cuts at: 826 1045 2473 3835 4970 5651 5842 826
Size: 219 1428 1362 1135 681 191 1471
Fragments arranged by size:
1471 1428 1362 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6487
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6487
SmaI CCC'GGG
Cuts at: 1872 3814 1872
Size: 1942 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4591 5492 5754 820
Size: 197 926 2648 901 262 1553
Fragments arranged by size:
2648 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4278 493
Size: 3785 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6487
SphI G_CATG'C
Cuts at: 1132 3845 1132
Size: 2713 3774
SspI AAT'ATT
Cuts at: 5 52 2407 4411 5
Size: 47 2355 2004 2081
Fragments arranged by size:
2355 2081 2004 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6487
StyI C'CwwG_G
Cuts at: 513 1065 2004 2442 3079 3123 3628 3926 513
Size: 552 939 438 637 44 505 298 3074
Fragments arranged by size:
3074 939 637 552 505 438 298 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2174 3056 4514 4531 4684 4869 5314
Size: 1255 594 882 1458 17 153 185 445
Cuts at: 5314 325
Size: 1498
Fragments arranged by size:
1498 1458 1255 882 594 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3033 4733 6445
Size: 274 80 33 51 1443 1056 1700 1712
Cuts at: 6445 96
Size: 138
Fragments arranged by size:
1712 1700 1443 1056 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6447 6479 6447
Size: 32 6455
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6487
XbaI T'CTAG_A
Cuts at: 3823 3823
Size: 6487
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6487
XmaI C'CCGG_G
Cuts at: 1870 3812 1870
Size: 1942 4545
XmnI GAAnn'nnTTC
Cuts at: 4616 4616
Size: 6487
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4295 276
Size: 53 83 186 936 2761 2468
Fragments arranged by size:
2761 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NruI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI
Bsu36I DraIII FalI FseI FspAI KasI NaeI NarI
NgoMIV PacI PmlI PspXI PsrI PvuII RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI