Restriction map for
pFN21AE4529
(Circular) (Six-base) MAPSORT of: pFN21AE4529.seq Check: 3930 from: 1 to: 6445
pFN21AE4529 6445 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2936 2936
Size: 6445
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3159 4255 278
Size: 53 83 186 936 1623 1096 2468
Fragments arranged by size:
2468 1623 1096 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3788 1937
Size: 1851 4594
Acc65I G'GTAC_C
Cuts at: 3766 3766
Size: 6445
AclI AA'CG_TT
Cuts at: 1563 4572 4945 1563
Size: 3009 373 3063
Fragments arranged by size:
3063 3009 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2735 2755 4508 5892 6160 1632
Size: 1103 20 1753 1384 268 1917
Fragments arranged by size:
1917 1753 1384 1103 268 20
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6248
AflIII A'CryG_T
Cuts at: 1203 2427 3420 5344 6321 1203
Size: 1224 993 1924 977 1327
Fragments arranged by size:
1924 1327 1224 993 977
AgeI A'CCGG_T
Cuts at: 5276 5276
Size: 6445
AhdI GACnn_n'nnGTC
Cuts at: 5174 5174
Size: 6445
AleI CACnn'nnGTG
Cuts at: 1166 2865 1166
Size: 1699 4746
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2219 1184
Size: 1035 5410
AlwNI CAG_nnn'CTG
Cuts at: 2413 4188 5760 2413
Size: 1775 1572 3098
Fragments arranged by size:
3098 1775 1572
ApaI G_GGCC'C
Cuts at: 3351 3351
Size: 6445
ApaLI G'TGCA_C
Cuts at: 4502 5658 4502
Size: 1156 5289
ApoI r'AATT_y
Cuts at: 1493 2675 3050 3754 3980 4132 6241 1493
Size: 1182 375 704 226 152 2109 1697
Fragments arranged by size:
2109 1697 1182 704 375 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4999 160
Size: 872 3967 1606
Fragments arranged by size:
3967 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6445
AvaI C'yCGr_G
Cuts at: 1870 1943 2063 2597 3103 3770 1870
Size: 73 120 534 506 667 4545
Fragments arranged by size:
4545 667 534 506 120 73
BamHI G'GATC_C
Cuts at: 2590 2814 3775 4142 2590
Size: 224 961 367 4893
Fragments arranged by size:
4893 961 367 224
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3107 3176 3560 3766 5221
Size: 325 217 627 1320 69 384 206 1455
Cuts at: 5221 618
Size: 1842
Fragments arranged by size:
1842 1455 1320 627 384 325 217 206
69
BanII G_rGCy'C
Cuts at: 1391 2259 3351 3764 1391
Size: 868 1092 413 4072
Fragments arranged by size:
4072 1092 868 413
BbeI G_GCGC'C
Cuts at: 3564 3564
Size: 6445
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2127 2723 2863 928
Size: 971 228 596 140 4510
Fragments arranged by size:
4510 971 596 228 140
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4636 4670 1903
Size: 34 2699 34 3678
Fragments arranged by size:
3678 2699 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4337 5547 4337
Size: 1210 5235
BclI T'GATC_A
Cuts at: 1548 2781 1548
Size: 1233 5212
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3446 5056 136
Size: 107 122 71 631 605 1774 1610 1525
Fragments arranged by size:
1774 1610 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6440 6440
Size: 6445
BlpI GC'TnA_GC
Cuts at: 3533 3862 3533
Size: 329 6116
Bme1580I G_kGCm'C
Cuts at: 1790 3110 3179 3351 4506 5662 1790
Size: 1320 69 172 1155 1156 2573
Fragments arranged by size:
2573 1320 1156 1155 172 69
BmgBI CAC'GTC
Cuts at: 1318 2428 2911 1318
Size: 1110 483 4852
Fragments arranged by size:
4852 1110 483
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2444 2859 4176
Size: 416 53 83 399 24 1027 415 1317
Cuts at: 4176 5134 442
Size: 958 1753
Fragments arranged by size:
1753 1317 1027 958 416 415 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6445
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2635 2714 2849 5105 1239
Size: 1396 79 135 2256 2579
Fragments arranged by size:
2579 2256 1396 135 79
Bpu10I CC'TnA_GC
Cuts at: 2485 2485
Size: 6445
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4570 5435 5733 4570
Size: 865 298 5282
Fragments arranged by size:
5282 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2030 2825 5108 882
Size: 617 531 795 2283 2219
Fragments arranged by size:
2283 2219 795 617 531
BsaAI yAC'GTr
Cuts at: 493 1204 2527 4236 493
Size: 711 1323 1709 2702
Fragments arranged by size:
2702 1709 1323 711
BsaBI GATnn'nnATC
Cuts at: 2819 3125 6439 2819
Size: 306 3314 2825
Fragments arranged by size:
3314 2825 306
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3156 3561 4252 4634
Size: 53 83 186 936 1623 405 691 382
Cuts at: 4634 5269 275
Size: 635 1451
Fragments arranged by size:
1623 1451 936 691 635 405 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4878 5276 5550 5697 6108 4878
Size: 398 274 147 411 5215
Fragments arranged by size:
5215 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2363 2393 3485 3515 1178
Size: 30 165 30 960 30 1092 30 4108
Fragments arranged by size:
4108 1092 960 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2936 3617 3737 1186
Size: 1750 681 120 3894
Fragments arranged by size:
3894 1750 681 120
BseYI C'CCAG_C
Cuts at: 2323 2618 3272 3343 3486 5648 6144 2323
Size: 295 654 71 143 2162 496 2624
Fragments arranged by size:
2624 2162 654 496 295 143 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2258 1163
Size: 573 522 5350
Fragments arranged by size:
5350 573 522
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3895 4656 5684 1540
Size: 275 186 1894 761 1028 2301
Fragments arranged by size:
2301 1894 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3096 3534 4506 4591 5662 3096
Size: 438 972 85 1071 3879
Fragments arranged by size:
3879 1071 972 438 85
BsmI GAATG_Cn'
Cuts at: 3727 3956 4049 3727
Size: 229 93 6123
Fragments arranged by size:
6123 229 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2141 2141
Size: 6445
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2259 3096 3110 3179 3351 3534 3764
Size: 399 469 837 14 69 172 183 230
Cuts at: 3764 4506 4591 5662 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 837 742 469 399 230 183
172 85 69 14
BspEI T'CCGG_A
Cuts at: 6108 6108
Size: 6445
BspHI T'CATG_A
Cuts at: 2151 3114 4334 6064 2151
Size: 963 1220 1730 2532
Fragments arranged by size:
2532 1730 1220 963
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2936 3800 844
Size: 2092 864 3489
Fragments arranged by size:
3489 2092 864
BsrBI CCG'CTC
Cuts at: 1394 1823 2044 2342 2602 2958 3043 3369 3675
Size: 429 221 298 260 356 85 326 306
Cuts at: 3675 4332 1394
Size: 657 3507
Fragments arranged by size:
3507 657 429 356 326 306 298 260
221 85
BsrDI GCAATG_nn'
Cuts at: 65 1248 4940 5114 65
Size: 1183 3692 174 1396
Fragments arranged by size:
3692 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2092 2439 3557 5089 5276 1700
Size: 110 282 347 1118 1532 187 2869
Fragments arranged by size:
2869 1532 1118 347 282 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6445
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6445
BssSI C'ACGA_G
Cuts at: 4505 5517 4505
Size: 1012 5433
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2193 3733 794
Size: 1399 1540 3506
Fragments arranged by size:
3506 1540 1399
BstBI TT'CG_AA
Cuts at: 5249 5249
Size: 6445
BstEII G'GTnAC_C
Cuts at: 1184 2465 1184
Size: 1281 5164
BstXI CCAn_nnnn'nTGG
Cuts at: 2827 3142 2827
Size: 315 6130
BstYI r'GATC_y
Cuts at: 1972 2220 2590 2751 2814 3775 4142 4527 4544
Size: 248 370 161 63 961 367 385 17
Cuts at: 4544 5996 6082 6094 6440 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 961 385 370 367 346 248
161 86 63 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2455 3378 3667 513
Size: 552 1390 923 289 3291
Fragments arranged by size:
3291 1390 923 552 289
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2284 3102 3232 3529 3610 487
Size: 683 56 1058 818 130 297 81 3322
Fragments arranged by size:
3322 1058 818 683 297 130 81 56
BtsI GCAGTG_nn'
Cuts at: 3254 3961 4755 4775 3254
Size: 707 794 20 4924
Fragments arranged by size:
4924 794 707 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6445
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6410
DraI TTT'AAA
Cuts at: 3749 3749
Size: 6445
DraIII CAC_nnn'GTG
Cuts at: 2807 2807
Size: 6445
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2915 5452 809
Size: 507 1599 2537 1802
Fragments arranged by size:
2537 1802 1599 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2535 3892 4781
Size: 54 1425 50 275 18 705 1357 889
Cuts at: 4781 6410 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1357 889 705 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3892 1537
Size: 275 2080 4090
Fragments arranged by size:
4090 2080 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2109 2283 2321 3485 3521 3545 4375 1353
Size: 756 174 38 1164 36 24 830 3423
Fragments arranged by size:
3423 1164 830 756 174 38 36 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2601 2827 3653 5032 5406 5552
Size: 1064 138 1012 226 826 1379 374 146
Cuts at: 5552 387
Size: 1280
Fragments arranged by size:
1379 1280 1064 1012 826 374 226 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2635 2714 2735 2755 2849 4508 5105
Size: 393 1003 79 21 20 94 1659 597
Cuts at: 5105 5892 6160 1239
Size: 787 268 1524
Fragments arranged by size:
1659 1524 1003 787 597 393 268 94
79 21 20
EcoO109I rG'GnC_Cy
Cuts at: 2073 2084 3347 3348 3712 2073
Size: 11 1263 1 364 4806
Fragments arranged by size:
4806 1263 364 11 1
EcoRI G'AATT_C
Cuts at: 3754 4132 3754
Size: 378 6067
FspI TGC'GCA
Cuts at: 4951 4951
Size: 6445
HaeII r_GCGC'y
Cuts at: 1678 3564 5592 1678
Size: 1886 2028 2531
Fragments arranged by size:
2531 2028 1886
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3012 3044 5086 5118 5160
Size: 32 135 32 1440 32 2042 32 42
Cuts at: 5160 5192 1373
Size: 32 2626
Fragments arranged by size:
2626 2042 1440 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3789 4035 669
Size: 988 281 1851 246 3079
Fragments arranged by size:
3079 1851 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6445
HpaI GTT'AAC
Cuts at: 4035 4035
Size: 6445
KasI G'GCGC_C
Cuts at: 3560 3560
Size: 6445
KpnI G_GTAC'C
Cuts at: 3770 3770
Size: 6445
MfeI C'AATT_G
Cuts at: 4044 4044
Size: 6445
MluI A'CGCG_T
Cuts at: 6321 6321
Size: 6445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2126 2332 5559 5743 1305
Size: 821 206 3227 184 2007
Fragments arranged by size:
3227 2007 821 206 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4623
Fragments arranged by size:
4623 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2585 2865 3113 3425 3524 4403 4762
Size: 648 1419 280 248 312 99 879 359
Cuts at: 4762 4921 518
Size: 159 2042
Fragments arranged by size:
2042 1419 879 648 359 312 280 248
159 99
MspA1I CmG'CkG
Cuts at: 1606 2269 2377 2506 3016 3669 3861 4538 5686
Size: 663 108 129 510 653 192 677 1148
Cuts at: 5686 5931 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 663 653 510 245 192
129 108
NarI GG'CG_CC
Cuts at: 3561 3561
Size: 6445
NcoI C'CATG_G
Cuts at: 513 1065 3378 513
Size: 552 2313 3580
Fragments arranged by size:
3580 2313 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6445
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6445
NotI GC'GGCC_GC
Cuts at: 3892 3892
Size: 6445
NspI r_CATG'y
Cuts at: 1132 1229 3424 3803 5348 1132
Size: 97 2195 379 1545 2229
Fragments arranged by size:
2229 2195 1545 379 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6445
PciI A'CATG_T
Cuts at: 3420 5344 3420
Size: 1924 4521
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2401 2413 2686 1108
Size: 1293 12 273 4867
Fragments arranged by size:
4867 1293 273 12
PfoI T'CCnGG_A
Cuts at: 2283 2754 2847 2283
Size: 471 93 5881
Fragments arranged by size:
5881 471 93
PmeI GTTT'AAAC
Cuts at: 3749 3749
Size: 6445
PmlI CAC'GTG
Cuts at: 2527 2527
Size: 6445
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3380 3412 4514 4546 6027 6059 3380
Size: 32 1102 32 1481 32 3766
Fragments arranged by size:
3766 1481 1102 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2073 3712 2073
Size: 1639 4806
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6445
PsiI TTA'TAA
Cuts at: 4015 4015
Size: 6445
PspOMI G'GGCC_C
Cuts at: 3347 3347
Size: 6445
PstI C_TGCA'G
Cuts at: 830 2985 3164 3618 3689 3797 830
Size: 2155 179 454 71 108 3478
Fragments arranged by size:
3478 2155 454 179 108 71
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6445
PvuII CAG'CTG
Cuts at: 2269 2377 2269
Size: 108 6337
SacII CC_GC'GG
Cuts at: 3670 3670
Size: 6445
SalI G'TCGA_C
Cuts at: 1936 3787 1936
Size: 1851 4594
SanDI GG'GwC_CC
Cuts at: 2073 3712 2073
Size: 1639 4806
SapI GCTCTTCn'nnn_
Cuts at: 3485 3485
Size: 6445
SbfI CC_TGCA'GG
Cuts at: 3797 3797
Size: 6445
ScaI AGT'ACT
Cuts at: 4693 6405 4693
Size: 1712 4733
SfcI C'TryA_G
Cuts at: 826 1045 2981 3160 3614 3685 3793 4928 5609
Size: 219 1936 179 454 71 108 1135 681
Cuts at: 5609 5800 826
Size: 191 1471
Fragments arranged by size:
1936 1471 1135 681 454 219 191 179
108 71
SfoI GGC'GCC
Cuts at: 3562 3562
Size: 6445
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6445
SgrAI Cr'CCGG_yG
Cuts at: 1700 3557 1700
Size: 1857 4588
SmaI CCC'GGG
Cuts at: 1872 2065 3772 1872
Size: 193 1707 4545
Fragments arranged by size:
4545 1707 193
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4549 5450 5712 820
Size: 197 926 2606 901 262 1553
Fragments arranged by size:
2606 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4236 493
Size: 3743 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6445
SphI G_CATG'C
Cuts at: 1132 3803 1132
Size: 2671 3774
SspI AAT'ATT
Cuts at: 5 52 4369 5
Size: 47 4317 2081
Fragments arranged by size:
4317 2081 47
StuI AGG'CCT
Cuts at: 1517 3620 1517
Size: 2103 4342
StyI C'CwwG_G
Cuts at: 513 1065 2178 2471 3378 3884 513
Size: 552 1113 293 907 506 3074
Fragments arranged by size:
3074 1113 907 552 506 293
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2054 3187 4472 4489 4642 4827 5272
Size: 1255 474 1133 1285 17 153 185 445
Cuts at: 5272 325
Size: 1498
Fragments arranged by size:
1498 1285 1255 1133 474 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4691 6403 96
Size: 274 80 33 51 1443 2714 1712 138
Fragments arranged by size:
2714 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6405 6437 6405
Size: 32 6413
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6445
XbaI T'CTAG_A
Cuts at: 3781 3781
Size: 6445
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3259 3259
Size: 6445
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6445
XmaI C'CCGG_G
Cuts at: 1870 2063 3770 1870
Size: 193 1707 4545
Fragments arranged by size:
4545 1707 193
XmnI GAAnn'nnTTC
Cuts at: 2124 4574 2124
Size: 2450 3995
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3157 4253 276
Size: 53 83 186 936 1623 1096 2468
Fragments arranged by size:
2468 1623 1096 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI
RsrII SacI SexAI SfiI SrfI SwaI