Restriction map for
pFN21AE4528
(Circular) (Six-base) MAPSORT of: pFN21AE4528.seq Check: 1973 from: 1 to: 6436
pFN21AE4528 6436 bp
With 182 enzymes: SgfI *
March 28, 2012 14:09 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4246 278
Size: 53 83 186 936 2710 2468
Fragments arranged by size:
2710 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2436 3779 1937
Size: 499 1343 4594
Fragments arranged by size:
4594 1343 499
Acc65I G'GTAC_C
Cuts at: 2993 3757 2993
Size: 764 5672
AclI AA'CG_TT
Cuts at: 1563 4563 4936 1563
Size: 3000 373 3063
Fragments arranged by size:
3063 3000 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4499 5883 6151 1632
Size: 2867 1384 268 1917
Fragments arranged by size:
2867 1917 1384 268
AfeI AGC'GCT
Cuts at: 2633 2633
Size: 6436
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6239
AflIII A'CryG_T
Cuts at: 1203 2439 2481 3405 5335 6312 1203
Size: 1236 42 924 1930 977 1327
Fragments arranged by size:
1930 1327 1236 977 924 42
AgeI A'CCGG_T
Cuts at: 5267 5267
Size: 6436
AhdI GACnn_n'nnGTC
Cuts at: 5165 5165
Size: 6436
AleI CACnn'nnGTG
Cuts at: 1166 2480 1166
Size: 1314 5122
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6436
AlwNI CAG_nnn'CTG
Cuts at: 2344 4179 5751 2344
Size: 1835 1572 3029
Fragments arranged by size:
3029 1835 1572
ApaLI G'TGCA_C
Cuts at: 4493 5649 4493
Size: 1156 5280
ApoI r'AATT_y
Cuts at: 1493 3745 3971 4123 6232 1493
Size: 2252 226 152 2109 1697
Fragments arranged by size:
2252 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4990 160
Size: 872 3958 1606
Fragments arranged by size:
3958 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6436
AvaI C'yCGr_G
Cuts at: 1870 1943 2775 3472 3761 1870
Size: 73 832 697 289 4545
Fragments arranged by size:
4545 832 697 289 73
BamHI G'GATC_C
Cuts at: 2569 2805 3766 4133 2569
Size: 236 961 367 4872
Fragments arranged by size:
4872 961 367 236
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2131 2537 2993 3021 3183
Size: 325 217 627 344 406 456 28 162
Cuts at: 3183 3189 3467 3638 3757 5212 618
Size: 6 278 171 119 1455 1842
Fragments arranged by size:
1842 1455 627 456 406 344 325 278
217 171 162 119 28 6
BanII G_rGCy'C
Cuts at: 1391 2751 3755 1391
Size: 1360 1004 4072
Fragments arranged by size:
4072 1360 1004
BbeI G_GCGC'C
Cuts at: 2541 3642 2541
Size: 1101 5335
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2446 928
Size: 971 547 4918
Fragments arranged by size:
4918 971 547
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4627 4661 1903
Size: 34 2690 34 3678
Fragments arranged by size:
3678 2690 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2917 4328 5538 2917
Size: 1411 1210 3815
Fragments arranged by size:
3815 1411 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6436
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2672 3338 3347
Size: 107 122 71 631 605 1000 666 9
Cuts at: 3347 3632 5047 136
Size: 285 1415 1525
Fragments arranged by size:
1525 1415 1000 666 631 605 285 122
107 71 9
BglII A'GATC_T
Cuts at: 6431 6431
Size: 6436
BlpI GC'TnA_GC
Cuts at: 3041 3119 3853 3041
Size: 78 734 5624
Fragments arranged by size:
5624 734 78
Bme1580I G_kGCm'C
Cuts at: 1790 3470 4497 5653 1790
Size: 1680 1027 1156 2573
Fragments arranged by size:
2573 1680 1156 1027
BmgBI CAC'GTC
Cuts at: 1318 2800 1318
Size: 1482 4954
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2866 3664 3688
Size: 416 53 83 399 24 1449 798 24
Cuts at: 3688 4167 5125 442
Size: 479 958 1753
Fragments arranged by size:
1753 1449 958 798 479 416 399 83
53 24 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6436
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3115 3115
Size: 6436
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 2765 3055 3607 5096 1239
Size: 1010 516 290 552 1489 2579
Fragments arranged by size:
2579 1489 1010 552 516 290
Bpu10I CC'TnA_GC
Cuts at: 2840 2840
Size: 6436
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2984 4561 5426 5724 2984
Size: 1577 865 298 3696
Fragments arranged by size:
3696 1577 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3226 5099 882
Size: 617 1727 1873 2219
Fragments arranged by size:
2219 1873 1727 617
BsaAI yAC'GTr
Cuts at: 493 1204 2478 2482 2698 3646 4227 493
Size: 711 1274 4 216 948 581 2702
Fragments arranged by size:
2702 1274 948 711 581 216 4
BsaBI GATnn'nnATC
Cuts at: 6430 6430
Size: 6436
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2348 2538 3095 3639
Size: 53 83 186 936 815 190 557 544
Cuts at: 3639 4243 4625 5260 275
Size: 604 382 635 1451
Fragments arranged by size:
1451 936 815 635 604 557 544 382
190 186 83 53
BsaWI w'CCGG_w
Cuts at: 4869 5267 5541 5688 6099 4869
Size: 398 274 147 411 5206
Fragments arranged by size:
5206 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6211
Fragments arranged by size:
6211 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6436
BseYI C'CCAG_C
Cuts at: 2634 3462 5639 6135 2634
Size: 828 2177 496 2935
Fragments arranged by size:
2935 2177 828 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2720 3335 1163
Size: 573 984 615 4264
Fragments arranged by size:
4264 984 615 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2149 3886 4647 5675 1540
Size: 275 186 148 1737 761 1028 2301
Fragments arranged by size:
2301 1737 1028 761 275 186 148
BsiHKAI G_wGCw'C
Cuts at: 2235 2751 4497 4582 5653 2235
Size: 516 1746 85 1071 3018
Fragments arranged by size:
3018 1746 1071 516 85
BsiWI C'GTAC_G
Cuts at: 2478 2478
Size: 6436
BsmI GAATG_Cn'
Cuts at: 3947 4040 3947
Size: 93 6343
BsmBI CGTCTCn'nnnn_
Cuts at: 3086 3086
Size: 6436
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2235 2751 3470 3755 4497 4582 5653
Size: 399 445 516 719 285 742 85 1071
Cuts at: 5653 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 719 516 445 399 285
85
BspEI T'CCGG_A
Cuts at: 6099 6099
Size: 6436
BspHI T'CATG_A
Cuts at: 2106 4325 6055 2106
Size: 2219 1730 2487
Fragments arranged by size:
2487 2219 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2340 2692 3791 844
Size: 1496 352 1099 3489
Fragments arranged by size:
3489 1496 1099 352
BsrBI CCG'CTC
Cuts at: 1394 1823 2878 3591 4323 1394
Size: 429 1055 713 732 3507
Fragments arranged by size:
3507 1055 732 713 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4931 5105 65
Size: 1183 3683 174 1396
Fragments arranged by size:
3683 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2235 3018 3186 3477 3635 3702 5080
Size: 110 425 783 168 291 158 67 1378
Cuts at: 5080 5267 1700
Size: 187 2869
Fragments arranged by size:
2869 1378 783 425 291 187 168 158
110 67
BsrGI T'GTAC_A
Cuts at: 96 2658 3165 96
Size: 2562 507 3367
Fragments arranged by size:
3367 2562 507
BssHII G'CGCG_C
Cuts at: 1925 2282 2374 2376 1925
Size: 357 92 2 5985
Fragments arranged by size:
5985 357 92 2
BssSI C'ACGA_G
Cuts at: 2750 3569 4496 5508 2750
Size: 819 927 1012 3678
Fragments arranged by size:
3678 1012 927 819
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6436
BstBI TT'CG_AA
Cuts at: 5240 5240
Size: 6436
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6436
BstXI CCAn_nnnn'nTGG
Cuts at: 3433 3433
Size: 6436
BstYI r'GATC_y
Cuts at: 1972 2569 2805 3766 4133 4518 4535 5987 6073
Size: 597 236 961 367 385 17 1452 86
Cuts at: 6073 6085 6431 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 961 597 385 367 346 236
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2517 2733 3627 513
Size: 552 1452 216 894 3322
Fragments arranged by size:
3322 1452 894 552 216
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2062 487
Size: 683 56 836 4861
Fragments arranged by size:
4861 836 683 56
BtsI GCAGTG_nn'
Cuts at: 2293 2592 2738 3952 4746 4766 2293
Size: 299 146 1214 794 20 3963
Fragments arranged by size:
3963 1214 794 299 146 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6436
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6401
DraI TTT'AAA
Cuts at: 3740 3740
Size: 6436
DraIII CAC_nnn'GTG
Cuts at: 2203 2203
Size: 6436
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2828 3100 3136 5443 809
Size: 507 1512 272 36 2307 1802
Fragments arranged by size:
2307 1802 1512 507 272 36
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3249 3330 3489
Size: 54 1425 50 275 18 1419 81 159
Cuts at: 3489 3624 3660 3883 4772 6401 8
Size: 135 36 223 889 1629 43
Fragments arranged by size:
1629 1425 1419 889 275 223 159 135
81 54 50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 3883 1537
Size: 275 2071 4090
Fragments arranged by size:
4090 2071 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2222 4366 1353
Size: 869 2144 3423
Fragments arranged by size:
3423 2144 869
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2296 2341 2579 3496 3724 5023
Size: 1064 138 707 45 238 917 228 1299
Cuts at: 5023 5397 5543 387
Size: 374 146 1280
Fragments arranged by size:
1299 1280 1064 917 707 374 238 228
146 138 45
EcoICRI GAG'CTC
Cuts at: 2749 2749
Size: 6436
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2765 3055 3607 4499 5096 5883
Size: 393 617 516 290 552 892 597 787
Cuts at: 5883 6151 1239
Size: 268 1524
Fragments arranged by size:
1524 892 787 617 597 552 516 393
290 268
EcoO109I rG'GnC_Cy
Cuts at: 2181 2320 2422 2467 2770 3075 3547 2181
Size: 139 102 45 303 305 472 5070
Fragments arranged by size:
5070 472 305 303 139 102 45
EcoRI G'AATT_C
Cuts at: 3745 4123 3745
Size: 378 6058
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3166 3358 3166
Size: 192 6244
FspI TGC'GCA
Cuts at: 3371 4942 3371
Size: 1571 4865
HaeII r_GCGC'y
Cuts at: 1678 2297 2541 2597 2635 2678 3293 3440 3524
Size: 619 244 56 38 43 615 147 84
Cuts at: 3524 3533 3642 5583 1678
Size: 9 109 1941 2531
Fragments arranged by size:
2531 1941 619 615 244 147 109 84
56 43 38 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5077 5109 5151 5183 1373
Size: 32 135 32 3505 32 42 32 2626
Fragments arranged by size:
3505 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2437 3669 3780 4026 669
Size: 988 281 499 1232 111 246 3079
Fragments arranged by size:
3079 1232 988 499 281 246 111
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6436
HpaI GTT'AAC
Cuts at: 4026 4026
Size: 6436
KasI G'GCGC_C
Cuts at: 2537 3638 2537
Size: 1101 5335
KpnI G_GTAC'C
Cuts at: 2997 3761 2997
Size: 764 5672
MfeI C'AATT_G
Cuts at: 4035 4035
Size: 6436
MluI A'CGCG_T
Cuts at: 6312 6312
Size: 6436
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2706 5550 5734 1305
Size: 1401 2844 184 2007
Fragments arranged by size:
2844 2007 1401 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3332 3662 10
Size: 54 1425 343 1500 330 2784
Fragments arranged by size:
2784 1500 1425 343 330 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2399 2480 4394 4753 4912 518
Size: 648 1233 81 1914 359 159 2042
Fragments arranged by size:
2042 1914 1233 648 359 159 81
MspA1I CmG'CkG
Cuts at: 1606 2046 2137 2266 2519 2919 3081 3629 3852
Size: 440 91 129 253 400 162 548 223
Cuts at: 3852 4529 5677 5922 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 548 440 400 253 245
223 162 129 91
NaeI GCC'GGC
Cuts at: 3479 3637 3704 3479
Size: 158 67 6211
Fragments arranged by size:
6211 158 67
NarI GG'CG_CC
Cuts at: 2538 3639 2538
Size: 1101 5335
NcoI C'CATG_G
Cuts at: 513 1065 2733 513
Size: 552 1668 4216
Fragments arranged by size:
4216 1668 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6436
NgoMIV G'CCGG_C
Cuts at: 3477 3635 3702 3477
Size: 158 67 6211
Fragments arranged by size:
6211 158 67
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6436
NotI GC'GGCC_GC
Cuts at: 3883 3883
Size: 6436
NspI r_CATG'y
Cuts at: 1132 1229 2443 3040 3409 3794 5339 1132
Size: 97 1214 597 369 385 1545 2229
Fragments arranged by size:
2229 1545 1214 597 385 369 97
PasI CC'CwG_GG
Cuts at: 1348 2508 1348
Size: 1160 5276
PciI A'CATG_T
Cuts at: 2439 3405 5335 2439
Size: 966 1930 3540
Fragments arranged by size:
3540 1930 966
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2344 1108
Size: 1236 5200
PmeI GTTT'AAAC
Cuts at: 3740 3740
Size: 6436
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4505 4537 6018 6050 4505
Size: 32 1481 32 4891
Fragments arranged by size:
4891 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2320 2422 2467 2770 3075 2320
Size: 102 45 303 305 5681
Fragments arranged by size:
5681 305 303 102 45
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6436
PsiI TTA'TAA
Cuts at: 4006 4006
Size: 6436
PstI C_TGCA'G
Cuts at: 830 2038 3788 830
Size: 1208 1750 3478
Fragments arranged by size:
3478 1750 1208
PvuI CG_AT'CG
Cuts at: 2001 2149 2001
Size: 148 6288
RsrII CG'GwC_CG
Cuts at: 3711 3711
Size: 6436
SacI G_AGCT'C
Cuts at: 2751 2751
Size: 6436
SacII CC_GC'GG
Cuts at: 2520 3630 2520
Size: 1110 5326
SalI G'TCGA_C
Cuts at: 1936 2435 3778 1936
Size: 499 1343 4594
Fragments arranged by size:
4594 1343 499
SbfI CC_TGCA'GG
Cuts at: 3788 3788
Size: 6436
ScaI AGT'ACT
Cuts at: 4684 6396 4684
Size: 1712 4724
SexAI A'CCwGG_T
Cuts at: 2199 2199
Size: 6436
SfcI C'TryA_G
Cuts at: 826 1045 2034 3784 4919 5600 5791 826
Size: 219 989 1750 1135 681 191 1471
Fragments arranged by size:
1750 1471 1135 989 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3338 3347 3632 3338
Size: 9 285 6142
Fragments arranged by size:
6142 285 9
SfoI GGC'GCC
Cuts at: 2539 3640 2539
Size: 1101 5335
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6436
SgrAI Cr'CCGG_yG
Cuts at: 1700 2235 1700
Size: 535 5901
SmaI CCC'GGG
Cuts at: 1872 2777 3763 1872
Size: 905 986 4545
Fragments arranged by size:
4545 986 905
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2963 4540 5441 5703 820
Size: 197 926 1020 1577 901 262 1553
Fragments arranged by size:
1577 1553 1020 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4227 493
Size: 3734 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6436
SphI G_CATG'C
Cuts at: 1132 3040 3794 1132
Size: 1908 754 3774
Fragments arranged by size:
3774 1908 754
SspI AAT'ATT
Cuts at: 5 52 4360 5
Size: 47 4308 2081
Fragments arranged by size:
4308 2081 47
StuI AGG'CCT
Cuts at: 1517 2931 2969 3614 1517
Size: 1414 38 645 4339
Fragments arranged by size:
4339 1414 645 38
StyI C'CwwG_G
Cuts at: 513 1065 2733 3284 3333 3875 513
Size: 552 1668 551 49 542 3074
Fragments arranged by size:
3074 1668 552 551 542 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3289 3646 3670 4463 4480 4633 4818
Size: 1255 1709 357 24 793 17 153 185
Cuts at: 4818 5263 325
Size: 445 1498
Fragments arranged by size:
1709 1498 1255 793 445 357 185 153
24 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2658 3002 3063
Size: 274 80 33 51 1443 681 344 61
Cuts at: 3063 3165 3675 4682 6394 96
Size: 102 510 1007 1712 138
Fragments arranged by size:
1712 1443 1007 681 510 344 274 138
102 80 61 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2891 2923 6396 6428 2891
Size: 32 3473 32 2899
Fragments arranged by size:
3473 2899 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6436
XbaI T'CTAG_A
Cuts at: 3772 3772
Size: 6436
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2740 3005 2740
Size: 265 6171
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6436
XmaI C'CCGG_G
Cuts at: 1870 2775 3761 1870
Size: 905 986 4545
Fragments arranged by size:
4545 986 905
XmnI GAAnn'nnTTC
Cuts at: 4565 4565
Size: 6436
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4244 276
Size: 53 83 186 936 2710 2468
Fragments arranged by size:
2710 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI RsrII SacI SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI PvuII SanDI
SapI SrfI SwaI