Restriction map for
pFN21AE4525
(Circular) (Six-base) MAPSORT of: pFN21AE4525.seq Check: 2215 from: 1 to: 6409
pFN21AE4525 6409 bp
With 182 enzymes: SgfI *
March 28, 2012 14:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2293 2293
Size: 6409
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4219 278
Size: 53 83 186 936 2683 2468
Fragments arranged by size:
2683 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3752 1937
Size: 1815 4594
Acc65I G'GTAC_C
Cuts at: 3512 3730 3512
Size: 218 6191
AclI AA'CG_TT
Cuts at: 1563 4536 4909 1563
Size: 2973 373 3063
Fragments arranged by size:
3063 2973 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4472 5856 6124 1632
Size: 2840 1384 268 1917
Fragments arranged by size:
2840 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6212
AflIII A'CryG_T
Cuts at: 1203 3363 5308 6285 1203
Size: 2160 1945 977 1327
Fragments arranged by size:
2160 1945 1327 977
AgeI A'CCGG_T
Cuts at: 5240 5240
Size: 6409
AhdI GACnn_n'nnGTC
Cuts at: 2293 3346 5138 2293
Size: 1053 1792 3564
Fragments arranged by size:
3564 1792 1053
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6409
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2760 1184
Size: 1576 4833
AlwNI CAG_nnn'CTG
Cuts at: 2146 2588 4152 5724 2146
Size: 442 1564 1572 2831
Fragments arranged by size:
2831 1572 1564 442
ApaI G_GGCC'C
Cuts at: 2243 2243
Size: 6409
ApaLI G'TGCA_C
Cuts at: 4466 5622 4466
Size: 1156 5253
ApoI r'AATT_y
Cuts at: 1493 2135 2578 3380 3718 3944 4096 6205 1493
Size: 642 443 802 338 226 152 2109 1697
Fragments arranged by size:
2109 1697 802 642 443 338 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4963 160
Size: 872 3931 1606
Fragments arranged by size:
3931 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6409
AvaI C'yCGr_G
Cuts at: 1870 1943 2236 2468 3734 1870
Size: 73 293 232 1266 4545
Fragments arranged by size:
4545 1266 293 232 73
BamHI G'GATC_C
Cuts at: 3486 3739 4106 3486
Size: 253 367 5789
Fragments arranged by size:
5789 367 253
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2473 2542 2740 3512 3730
Size: 325 217 627 686 69 198 772 218
Cuts at: 3730 5185 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 772 686 627 325 218 217
198 69
BanII G_rGCy'C
Cuts at: 1391 2235 2243 2346 2451 2625 2733 3728 1391
Size: 844 8 103 105 174 108 995 4072
Fragments arranged by size:
4072 995 844 174 108 105 103 8
BbeI G_GCGC'C
Cuts at: 2546 2744 2546
Size: 198 6211
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3506 928
Size: 971 1607 3831
Fragments arranged by size:
3831 1607 971
BbvCI CC'TCA_GC
Cuts at: 2538 2646 2538
Size: 108 6301
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3601 3635 4600 4634 1903
Size: 34 1664 34 965 34 3678
Fragments arranged by size:
3678 1664 965 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4301 5511 4301
Size: 1210 5199
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6409
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2117 2678 5020
Size: 107 122 71 631 605 445 561 2342
Cuts at: 5020 136
Size: 1525
Fragments arranged by size:
2342 1525 631 605 561 445 122 107
71
BglII A'GATC_T
Cuts at: 2036 6404 2036
Size: 4368 2041
BlpI GC'TnA_GC
Cuts at: 3826 3826
Size: 6409
Bme1580I G_kGCm'C
Cuts at: 1790 2243 2478 4470 5626 1790
Size: 453 235 1992 1156 2573
Fragments arranged by size:
2573 1992 1156 453 235
BmgBI CAC'GTC
Cuts at: 1318 3364 1318
Size: 2046 4363
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4140 5098 442
Size: 416 53 83 399 24 2723 958 1753
Fragments arranged by size:
2723 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6409
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2639 2825 3323 5069 1239
Size: 1400 186 498 1746 2579
Fragments arranged by size:
2579 1746 1400 498 186
Bpu10I CC'TnA_GC
Cuts at: 2192 2538 2646 3248 2192
Size: 346 108 602 5353
Fragments arranged by size:
5353 602 346 108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2330 2717 4534 5399 5697 2330
Size: 387 1817 865 298 3042
Fragments arranged by size:
3042 1817 865 387 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5072 882
Size: 617 3573 2219
Fragments arranged by size:
3573 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2785 4200 493
Size: 711 1581 1415 2702
Fragments arranged by size:
2702 1581 1415 711
BsaBI GATnn'nnATC
Cuts at: 2408 6403 2408
Size: 3995 2414
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2213 2375 2543 2741
Size: 53 83 186 936 680 162 168 198
Cuts at: 2741 3009 4216 4598 5233 275
Size: 268 1207 382 635 1451
Fragments arranged by size:
1451 1207 936 680 635 382 268 198
186 168 162 83 53
BsaWI w'CCGG_w
Cuts at: 2016 2436 3012 4842 5240 5514 5661 6072 2016
Size: 420 576 1830 398 274 147 411 2353
Fragments arranged by size:
2353 1830 576 420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6184
Fragments arranged by size:
6184 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 2963 3014 3143 3320 3695 1186
Size: 880 897 51 129 177 375 3900
Fragments arranged by size:
3900 897 880 375 177 129 51
BseYI C'CCAG_C
Cuts at: 2555 2560 2631 2687 5612 6108 2555
Size: 5 71 56 2925 496 2856
Fragments arranged by size:
2925 2856 496 71 56 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2954 1163
Size: 573 1218 4618
Fragments arranged by size:
4618 1218 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2253 3859 4620 5648 1540
Size: 275 186 252 1606 761 1028 2301
Fragments arranged by size:
2301 1606 1028 761 275 252 186
BsiHKAI G_wGCw'C
Cuts at: 2235 4470 4555 5626 2235
Size: 2235 85 1071 3018
Fragments arranged by size:
3018 2235 1071 85
BsmI GAATG_Cn'
Cuts at: 3920 4013 3920
Size: 93 6316
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2235 2243 2346 2451 2478 2625 2733
Size: 399 445 8 103 105 27 147 108
Cuts at: 2733 3728 4470 4555 5626 1391
Size: 995 742 85 1071 2174
Fragments arranged by size:
2174 1071 995 742 445 399 147 108
105 103 85 27 8
BspEI T'CCGG_A
Cuts at: 3012 6072 3012
Size: 3060 3349
BspHI T'CATG_A
Cuts at: 3087 4298 6028 3087
Size: 1211 1730 3468
Fragments arranged by size:
3468 1730 1211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2293 3129 3351 3564 3764 844
Size: 1449 836 222 213 200 3489
Fragments arranged by size:
3489 1449 836 222 213 200
BsrBI CCG'CTC
Cuts at: 1394 1823 2871 4296 1394
Size: 429 1048 1425 3507
Fragments arranged by size:
3507 1425 1048 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4904 5078 65
Size: 1183 3656 174 1396
Fragments arranged by size:
3656 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2257 2383 3203 3402 5053 5240 1700
Size: 110 447 126 820 199 1651 187 2869
Fragments arranged by size:
2869 1651 820 447 199 187 126 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6409
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6409
BssSI C'ACGA_G
Cuts at: 3461 4469 5481 3461
Size: 1008 1012 4389
Fragments arranged by size:
4389 1012 1008
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6409
BstBI TT'CG_AA
Cuts at: 5213 5213
Size: 6409
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6409
BstXI CCAn_nnnn'nTGG
Cuts at: 2305 2512 3676 2305
Size: 207 1164 5038
Fragments arranged by size:
5038 1164 207
BstYI r'GATC_y
Cuts at: 1972 2019 2036 2517 3486 3739 4106 4491 4508
Size: 47 17 481 969 253 367 385 17
Cuts at: 4508 5960 6046 6058 6404 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 969 481 385 367 346 253
86 47 17 17 12
Bsu36I CC'TnA_GG
Cuts at: 3215 3215
Size: 6409
BtgI C'CryG_G
Cuts at: 513 1065 2004 3663 513
Size: 552 939 1659 3259
Fragments arranged by size:
3259 1659 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3262 487
Size: 683 56 2036 3634
Fragments arranged by size:
3634 2036 683 56
BtsI GCAGTG_nn'
Cuts at: 2478 3175 3460 3925 4719 4739 2478
Size: 697 285 465 794 20 4148
Fragments arranged by size:
4148 794 697 465 285 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6409
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6374
DraI TTT'AAA
Cuts at: 3713 3713
Size: 6409
DraIII CAC_nnn'GTG
Cuts at: 3542 3542
Size: 6409
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3170 5416 809
Size: 507 1854 2246 1802
Fragments arranged by size:
2246 1854 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2118 2250 2706
Size: 54 1425 50 275 18 288 132 456
Cuts at: 2706 3205 3666 3856 4745 6374 8
Size: 499 461 190 889 1629 43
Fragments arranged by size:
1629 1425 889 499 461 456 288 275
190 132 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2250 3856 1537
Size: 275 438 1606 4090
Fragments arranged by size:
4090 1606 438 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2909 4339 1353
Size: 1556 1430 3423
Fragments arranged by size:
3423 1556 1430
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3302 4996 5370 5516 387
Size: 1064 138 1713 1694 374 146 1280
Fragments arranged by size:
1713 1694 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2233 2233
Size: 6409
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2639 2825 3323 4472 5069 5856 6124
Size: 393 1007 186 498 1149 597 787 268
Cuts at: 6124 1239
Size: 1524
Fragments arranged by size:
1524 1149 1007 787 597 498 393 268
186
EcoNI CCTnn'n_nnAGG
Cuts at: 3125 3251 3647 3125
Size: 126 396 5887
Fragments arranged by size:
5887 396 126
EcoO109I rG'GnC_Cy
Cuts at: 2143 2240 2464 2764 3219 3562 2143
Size: 97 224 300 455 343 4990
Fragments arranged by size:
4990 455 343 300 224 97
EcoRI G'AATT_C
Cuts at: 2578 3718 4096 2578
Size: 1140 378 4891
Fragments arranged by size:
4891 1140 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2854 2854
Size: 6409
FspI TGC'GCA
Cuts at: 4915 4915
Size: 6409
HaeII r_GCGC'y
Cuts at: 1678 2546 2744 3643 3663 5556 1678
Size: 868 198 899 20 1893 2531
Fragments arranged by size:
2531 1893 899 868 198 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2006 2038 2246 2278 2279
Size: 32 135 32 434 32 208 32 1
Cuts at: 2279 2311 2963 2995 3332 3364 3377 3409 5050
Size: 32 652 32 337 32 13 32 1641
Cuts at: 5050 5082 5124 5156 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1641 652 434 337 208 135 42
32 32 32 32 32 32 32 32
32 32 13 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3136 3262 3475 3753 3999 669
Size: 988 281 1198 126 213 278 246 3079
Fragments arranged by size:
3079 1198 988 281 278 246 213 126
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6409
HpaI GTT'AAC
Cuts at: 3999 3999
Size: 6409
KasI G'GCGC_C
Cuts at: 2542 2740 2542
Size: 198 6211
KpnI G_GTAC'C
Cuts at: 3516 3734 3516
Size: 218 6191
MfeI C'AATT_G
Cuts at: 4008 4008
Size: 6409
MluI A'CGCG_T
Cuts at: 6285 6285
Size: 6409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3375 5523 5707 1305
Size: 2070 2148 184 2007
Fragments arranged by size:
2148 2070 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2120 3668 10
Size: 54 1425 343 288 1548 2751
Fragments arranged by size:
2751 1548 1425 343 288 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2855 3362 4367 4726 4885 518
Size: 648 1689 507 1005 359 159 2042
Fragments arranged by size:
2042 1689 1005 648 507 359 159
MspA1I CmG'CkG
Cuts at: 1606 2605 2691 3328 3825 4502 5650 5895 1606
Size: 999 86 637 497 677 1148 245 2120
Fragments arranged by size:
2120 1148 999 677 637 497 245 86
NarI GG'CG_CC
Cuts at: 2543 2741 2543
Size: 198 6211
NcoI C'CATG_G
Cuts at: 513 1065 2004 3663 513
Size: 552 939 1659 3259
Fragments arranged by size:
3259 1659 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6409
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6409
NotI GC'GGCC_GC
Cuts at: 3856 3856
Size: 6409
NspI r_CATG'y
Cuts at: 1132 1229 3586 3767 5312 1132
Size: 97 2357 181 1545 2229
Fragments arranged by size:
2357 2229 1545 181 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6409
PciI A'CATG_T
Cuts at: 5308 5308
Size: 6409
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2146 2572 1108
Size: 1038 426 4945
Fragments arranged by size:
4945 1038 426
PfoI T'CCnGG_A
Cuts at: 2139 2802 3159 3423 2139
Size: 663 357 264 5125
Fragments arranged by size:
5125 663 357 264
PmeI GTTT'AAAC
Cuts at: 3713 3713
Size: 6409
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4478 4510 5991 6023 4478
Size: 32 1481 32 4864
Fragments arranged by size:
4864 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2143 2464 3562 2143
Size: 321 1098 4990
Fragments arranged by size:
4990 1098 321
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6409
PsiI TTA'TAA
Cuts at: 3979 3979
Size: 6409
PspOMI G'GGCC_C
Cuts at: 2239 2239
Size: 6409
PstI C_TGCA'G
Cuts at: 830 2610 3126 3761 830
Size: 1780 516 635 3478
Fragments arranged by size:
3478 1780 635 516
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6409
PvuII CAG'CTG
Cuts at: 2605 2691 2605
Size: 86 6323
SacI G_AGCT'C
Cuts at: 2235 2235
Size: 6409
SalI G'TCGA_C
Cuts at: 1936 3751 1936
Size: 1815 4594
SbfI CC_TGCA'GG
Cuts at: 3126 3761 3126
Size: 635 5774
ScaI AGT'ACT
Cuts at: 4657 6369 4657
Size: 1712 4697
SexAI A'CCwGG_T
Cuts at: 3468 3468
Size: 6409
SfcI C'TryA_G
Cuts at: 826 1045 2606 3122 3757 4892 5573 5764 826
Size: 219 1561 516 635 1135 681 191 1471
Fragments arranged by size:
1561 1471 1135 681 635 516 219 191
SfoI GGC'GCC
Cuts at: 2544 2742 2544
Size: 198 6211
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6409
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6409
SmaI CCC'GGG
Cuts at: 1872 3736 1872
Size: 1864 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2345 2732 4513 5414 5676 820
Size: 197 926 402 387 1781 901 262 1553
Fragments arranged by size:
1781 1553 926 901 402 387 262 197
SnaBI TAC'GTA
Cuts at: 493 4200 493
Size: 3707 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6409
SphI G_CATG'C
Cuts at: 1132 3767 1132
Size: 2635 3774
SspI AAT'ATT
Cuts at: 5 52 4333 5
Size: 47 4281 2081
Fragments arranged by size:
4281 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6409
StyI C'CwwG_G
Cuts at: 513 1065 2004 2208 2244 2460 2571 3282 3490
Size: 552 939 204 36 216 111 711 208
Cuts at: 3490 3663 3848 513
Size: 173 185 3074
Fragments arranged by size:
3074 939 711 552 216 208 204 185
173 111 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2080 2144 4436 4453 4606 4791 5236
Size: 1255 500 64 2292 17 153 185 445
Cuts at: 5236 325
Size: 1498
Fragments arranged by size:
2292 1498 1255 500 445 185 153 64
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4655 6367 96
Size: 274 80 33 51 1443 2678 1712 138
Fragments arranged by size:
2678 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6369 6401 6369
Size: 32 6377
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6409
XbaI T'CTAG_A
Cuts at: 3745 3745
Size: 6409
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6409
XmaI C'CCGG_G
Cuts at: 1870 3734 1870
Size: 1864 4545
XmnI GAAnn'nnTTC
Cuts at: 4538 4538
Size: 6409
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4217 276
Size: 53 83 186 936 2683 2468
Fragments arranged by size:
2683 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI
SapI SfiI SrfI SwaI XcmI