Restriction map for
pFN21AE4524
(Circular) (Six-base) MAPSORT of: pFN21AE4524.seq Check: 5774 from: 1 to: 6403
pFN21AE4524 6403 bp
With 182 enzymes: SgfI *
March 19, 2012 14:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4213 278
Size: 53 83 186 936 2677 2468
Fragments arranged by size:
2677 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3746 1937
Size: 1809 4594
Acc65I G'GTAC_C
Cuts at: 3724 3724
Size: 6403
AclI AA'CG_TT
Cuts at: 1563 4530 4903 1563
Size: 2967 373 3063
Fragments arranged by size:
3063 2967 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2364 2521 3021 3638 4466 5850 6118 1632
Size: 732 157 500 617 828 1384 268 1917
Fragments arranged by size:
1917 1384 828 732 617 500 268 157
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6206
AflIII A'CryG_T
Cuts at: 1203 3336 5302 6279 1203
Size: 2133 1966 977 1327
Fragments arranged by size:
2133 1966 1327 977
AgeI A'CCGG_T
Cuts at: 5234 5234
Size: 6403
AhdI GACnn_n'nnGTC
Cuts at: 5132 5132
Size: 6403
AleI CACnn'nnGTG
Cuts at: 1166 3145 1166
Size: 1979 4424
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3606 1184
Size: 2422 3981
AlwNI CAG_nnn'CTG
Cuts at: 2645 3616 4146 5718 2645
Size: 971 530 1572 3330
Fragments arranged by size:
3330 1572 971 530
ApaI G_GGCC'C
Cuts at: 3436 3436
Size: 6403
ApaLI G'TGCA_C
Cuts at: 2263 4460 5616 2263
Size: 2197 1156 3050
Fragments arranged by size:
3050 2197 1156
ApoI r'AATT_y
Cuts at: 1493 2909 3022 3494 3712 3938 4090 6199 1493
Size: 1416 113 472 218 226 152 2109 1697
Fragments arranged by size:
2109 1697 1416 472 226 218 152 113
AseI AT'TA_AT
Cuts at: 160 1032 4957 160
Size: 872 3925 1606
Fragments arranged by size:
3925 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6403
AvaI C'yCGr_G
Cuts at: 1870 1943 3728 1870
Size: 73 1785 4545
Fragments arranged by size:
4545 1785 73
BamHI G'GATC_C
Cuts at: 2418 3733 4100 2418
Size: 1315 367 4721
Fragments arranged by size:
4721 1315 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3452 3590 3724 5179 618
Size: 325 217 627 1665 138 134 1455 1842
Fragments arranged by size:
1842 1665 1455 627 325 217 138 134
BanII G_rGCy'C
Cuts at: 1391 2886 3417 3436 3722 1391
Size: 1495 531 19 286 4072
Fragments arranged by size:
4072 1495 531 286 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2352 2876 3134 928
Size: 971 453 524 258 4197
Fragments arranged by size:
4197 971 524 453 258
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4594 4628 1903
Size: 34 2657 34 3678
Fragments arranged by size:
3678 2657 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4295 5505 4295
Size: 1210 5193
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6403
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3550 3662 5014
Size: 107 122 71 631 605 1878 112 1352
Cuts at: 5014 136
Size: 1525
Fragments arranged by size:
1878 1525 1352 631 605 122 112 107
71
BglII A'GATC_T
Cuts at: 6398 6398
Size: 6403
BlpI GC'TnA_GC
Cuts at: 2799 3820 2799
Size: 1021 5382
Bme1580I G_kGCm'C
Cuts at: 1790 2267 2966 3436 4464 5620 1790
Size: 477 699 470 1028 1156 2573
Fragments arranged by size:
2573 1156 1028 699 477 470
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6403
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2578 4134 5092
Size: 416 53 83 399 24 1161 1556 958
Cuts at: 5092 442
Size: 1753
Fragments arranged by size:
1753 1556 1161 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6403
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2217 3436 3472 2217
Size: 1219 36 5148
Fragments arranged by size:
5148 1219 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3342 5063 1239
Size: 2103 1721 2579
Fragments arranged by size:
2579 2103 1721
Bpu10I CC'TnA_GC
Cuts at: 3177 3177
Size: 6403
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2318 4528 5393 5691 2318
Size: 2210 865 298 3030
Fragments arranged by size:
3030 2210 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2928 3393 5066 882
Size: 617 1429 465 1673 2219
Fragments arranged by size:
2219 1673 1429 617 465
BsaAI yAC'GTr
Cuts at: 493 1204 4194 493
Size: 711 2990 2702
Fragments arranged by size:
2990 2702 711
BsaBI GATnn'nnATC
Cuts at: 6397 6397
Size: 6403
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4210 4592 5227 275
Size: 53 83 186 936 2677 382 635 1451
Fragments arranged by size:
2677 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4836 5234 5508 5655 6066 4836
Size: 398 274 147 411 5173
Fragments arranged by size:
5173 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2667 2697 1178
Size: 30 165 30 1264 30 4884
Fragments arranged by size:
4884 1264 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2688 2894 3479 1186
Size: 1502 206 585 4110
Fragments arranged by size:
4110 1502 585 206
BseYI C'CCAG_C
Cuts at: 2104 2601 2819 5606 6102 2104
Size: 497 218 2787 496 2405
Fragments arranged by size:
2787 2405 497 496 218
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5830
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3853 4614 5642 1540
Size: 275 186 1852 761 1028 2301
Fragments arranged by size:
2301 1852 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2267 2886 3328 3417 4464 4549 5620 2267
Size: 619 442 89 1047 85 1071 3050
Fragments arranged by size:
3050 1071 1047 619 442 89 85
BsmI GAATG_Cn'
Cuts at: 2245 3914 4007 2245
Size: 1669 93 4641
Fragments arranged by size:
4641 1669 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2885 2885
Size: 6403
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2267 2886 2966 3328 3417 3436 3722
Size: 399 477 619 80 362 89 19 286
Cuts at: 3722 4464 4549 5620 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 619 477 399 362 286
89 85 80 19
BspEI T'CCGG_A
Cuts at: 6066 6066
Size: 6403
BspHI T'CATG_A
Cuts at: 4292 6022 4292
Size: 1730 4673
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3758 844
Size: 2914 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4290 1394
Size: 429 2467 3507
Fragments arranged by size:
3507 2467 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2616 3171 3523 4898 5072 65
Size: 1183 1368 555 352 1375 174 1396
Fragments arranged by size:
1396 1375 1368 1183 555 352 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2404 5047 5234 1700
Size: 110 594 2643 187 2869
Fragments arranged by size:
2869 2643 594 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6403
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6403
BssSI C'ACGA_G
Cuts at: 4463 5475 4463
Size: 1012 5391
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6403
BstBI TT'CG_AA
Cuts at: 5207 5207
Size: 6403
BstEII G'GTnAC_C
Cuts at: 1184 2500 2573 1184
Size: 1316 73 5014
Fragments arranged by size:
5014 1316 73
BstXI CCAn_nnnn'nTGG
Cuts at: 2155 2155
Size: 6403
BstYI r'GATC_y
Cuts at: 1972 2272 2418 3733 4100 4485 4502 5954 6040
Size: 300 146 1315 367 385 17 1452 86
Cuts at: 6040 6052 6398 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1315 385 367 346 300 146
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2192 2192
Size: 6403
BtgI C'CryG_G
Cuts at: 513 1065 2046 2222 2422 3403 513
Size: 552 981 176 200 981 3513
Fragments arranged by size:
3513 981 981 552 200 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5664
Fragments arranged by size:
5664 683 56
BtsI GCAGTG_nn'
Cuts at: 2814 2854 3672 3919 4713 4733 2814
Size: 40 818 247 794 20 4484
Fragments arranged by size:
4484 818 794 247 40 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6403
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6368
DraI TTT'AAA
Cuts at: 3444 3707 3444
Size: 263 6140
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3136 3230 5410 809
Size: 507 1820 94 2180 1802
Fragments arranged by size:
2180 1820 1802 507 94
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2145 2709
Size: 54 1425 50 275 18 198 117 564
Cuts at: 2709 3551 3850 4739 6368 8
Size: 842 299 889 1629 43
Fragments arranged by size:
1629 1425 889 842 564 299 275 198
117 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3850 1537
Size: 275 2038 4090
Fragments arranged by size:
4090 2038 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2726 2871 2997 3422 3456 4333 1353
Size: 1373 145 126 425 34 877 3423
Fragments arranged by size:
3423 1373 877 425 145 126 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4990 5364 5510 387
Size: 1064 138 3401 374 146 1280
Fragments arranged by size:
3401 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2884 3415 2884
Size: 531 5872
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2364 2521 3021 3342 3638 4466 5063
Size: 393 732 157 500 321 296 828 597
Cuts at: 5063 5850 6118 1239
Size: 787 268 1524
Fragments arranged by size:
1524 828 787 732 597 500 393 321
296 268 157
EcoNI CCTnn'n_nnAGG
Cuts at: 2582 2582
Size: 6403
EcoO109I rG'GnC_Cy
Cuts at: 3432 3433 3432
Size: 1 6402
EcoRI G'AATT_C
Cuts at: 3712 4090 3712
Size: 378 6025
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2369 2737 2369
Size: 368 6035
FspI TGC'GCA
Cuts at: 4909 4909
Size: 6403
HaeII r_GCGC'y
Cuts at: 1678 5550 1678
Size: 3872 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2667 2699 3082 3114 5044
Size: 32 135 32 1095 32 383 32 1930
Cuts at: 5044 5076 5118 5150 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1930 1095 383 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2951 3747 3993 669
Size: 988 281 1013 796 246 3079
Fragments arranged by size:
3079 1013 988 796 281 246
HindIII A'AGCT_T
Cuts at: 748 2336 3361 748
Size: 1588 1025 3790
Fragments arranged by size:
3790 1588 1025
HpaI GTT'AAC
Cuts at: 3993 3993
Size: 6403
KpnI G_GTAC'C
Cuts at: 3728 3728
Size: 6403
MfeI C'AATT_G
Cuts at: 4002 4002
Size: 6403
MluI A'CGCG_T
Cuts at: 6279 6279
Size: 6403
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3028 5517 5701 1305
Size: 1723 2489 184 2007
Fragments arranged by size:
2489 2007 1723 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2030 2147 2711 3553 10
Size: 54 1425 343 198 117 564 842 2860
Fragments arranged by size:
2860 1425 842 564 343 198 117 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3145 3315 3335 3408 4361 4720 4879
Size: 648 1979 170 20 73 953 359 159
Cuts at: 4879 518
Size: 2042
Fragments arranged by size:
2042 1979 953 648 359 170 159 73
20
MspA1I CmG'CkG
Cuts at: 1606 2048 2669 3819 4496 5644 5889 1606
Size: 442 621 1150 677 1148 245 2120
Fragments arranged by size:
2120 1150 1148 677 621 442 245
NcoI C'CATG_G
Cuts at: 513 1065 2222 513
Size: 552 1157 4694
Fragments arranged by size:
4694 1157 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6403
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6403
NotI GC'GGCC_GC
Cuts at: 3850 3850
Size: 6403
NspI r_CATG'y
Cuts at: 1132 1229 3340 3761 5306 1132
Size: 97 2111 421 1545 2229
Fragments arranged by size:
2229 2111 1545 421 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6403
PciI A'CATG_T
Cuts at: 3336 5302 3336
Size: 1966 4437
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6403
PfoI T'CCnGG_A
Cuts at: 2607 3252 2607
Size: 645 5758
PmeI GTTT'AAAC
Cuts at: 3707 3707
Size: 6403
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2627 2659 4472 4504 5985 6017 2627
Size: 32 1813 32 1481 32 3013
Fragments arranged by size:
3013 1813 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6403
PsiI TTA'TAA
Cuts at: 3973 3973
Size: 6403
PspOMI G'GGCC_C
Cuts at: 3432 3432
Size: 6403
PstI C_TGCA'G
Cuts at: 830 2218 3585 3755 830
Size: 1388 1367 170 3478
Fragments arranged by size:
3478 1388 1367 170
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6403
SacI G_AGCT'C
Cuts at: 2886 3417 2886
Size: 531 5872
SacII CC_GC'GG
Cuts at: 2049 2049
Size: 6403
SalI G'TCGA_C
Cuts at: 1936 3745 1936
Size: 1809 4594
SapI GCTCTTCn'nnn_
Cuts at: 2997 3422 2997
Size: 425 5978
SbfI CC_TGCA'GG
Cuts at: 3755 3755
Size: 6403
ScaI AGT'ACT
Cuts at: 4651 6363 4651
Size: 1712 4691
SexAI A'CCwGG_T
Cuts at: 2583 2583
Size: 6403
SfcI C'TryA_G
Cuts at: 826 1045 2214 3581 3751 4886 5567 5758 826
Size: 219 1169 1367 170 1135 681 191 1471
Fragments arranged by size:
1471 1367 1169 1135 681 219 191 170
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6403
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6403
SmaI CCC'GGG
Cuts at: 1872 3730 1872
Size: 1858 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2333 4507 5408 5670 820
Size: 197 926 390 2174 901 262 1553
Fragments arranged by size:
2174 1553 926 901 390 262 197
SnaBI TAC'GTA
Cuts at: 493 4194 493
Size: 3701 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6403
SphI G_CATG'C
Cuts at: 1132 3761 1132
Size: 2629 3774
SspI AAT'ATT
Cuts at: 5 52 2365 4327 5
Size: 47 2313 1962 2081
Fragments arranged by size:
2313 2081 1962 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6403
StyI C'CwwG_G
Cuts at: 513 1065 2222 2277 2793 3842 513
Size: 552 1157 55 516 1049 3074
Fragments arranged by size:
3074 1157 1049 552 516 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2282 2432 2811 3343 3614 4430 4447
Size: 1255 702 150 379 532 271 816 17
Cuts at: 4447 4600 4785 5230 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 816 702 532 445 379 271
185 153 150 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3558 4649 6361
Size: 274 80 33 51 1443 1581 1091 1712
Cuts at: 6361 96
Size: 138
Fragments arranged by size:
1712 1581 1443 1091 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2257 2289 6363 6395 2257
Size: 32 4074 32 2265
Fragments arranged by size:
4074 2265 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3230 1316
Size: 1914 4489
XbaI T'CTAG_A
Cuts at: 3739 3739
Size: 6403
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3317 3664 3317
Size: 347 6056
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6403
XmaI C'CCGG_G
Cuts at: 1870 3728 1870
Size: 1858 4545
XmnI GAAnn'nnTTC
Cuts at: 2720 3006 3186 4532 2720
Size: 286 180 1346 4591
Fragments arranged by size:
4591 1346 286 180
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4211 276
Size: 53 83 186 936 2677 2468
Fragments arranged by size:
2677 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspXI PsrI PvuII RsrII SanDI SfiI SfoI SrfI
SwaI