Restriction map for
pFN21AE4522
(Circular) (Six-base) MAPSORT of: pFN21AE4522.seq Check: 1027 from: 1 to: 6391
pFN21AE4522 6391 bp
With 182 enzymes: SgfI *
March 28, 2012 14:08 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4201 278
Size: 53 83 186 936 2665 2468
Fragments arranged by size:
2665 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2499 3734 1937
Size: 562 1235 4594
Fragments arranged by size:
4594 1235 562
Acc65I G'GTAC_C
Cuts at: 3712 3712
Size: 6391
AclI AA'CG_TT
Cuts at: 1563 4518 4891 1563
Size: 2955 373 3063
Fragments arranged by size:
3063 2955 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2678 2907 2941 4454 5838 6106 1632
Size: 1046 229 34 1513 1384 268 1917
Fragments arranged by size:
1917 1513 1384 1046 268 229 34
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6194
AflIII A'CryG_T
Cuts at: 1203 5290 6267 1203
Size: 4087 977 1327
Fragments arranged by size:
4087 1327 977
AgeI A'CCGG_T
Cuts at: 3146 5222 3146
Size: 2076 4315
AhdI GACnn_n'nnGTC
Cuts at: 2352 5120 2352
Size: 2768 3623
AleI CACnn'nnGTG
Cuts at: 1166 2174 3162 1166
Size: 1008 988 4395
Fragments arranged by size:
4395 1008 988
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2770 1184
Size: 1586 4805
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2180 2212 2528 2560 2180
Size: 32 316 32 6011
Fragments arranged by size:
6011 316 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2930 4134 5706 2930
Size: 1204 1572 3615
Fragments arranged by size:
3615 1572 1204
ApaI G_GGCC'C
Cuts at: 2372 2372
Size: 6391
ApaLI G'TGCA_C
Cuts at: 2622 4448 5604 2622
Size: 1826 1156 3409
Fragments arranged by size:
3409 1826 1156
ApoI r'AATT_y
Cuts at: 1493 2393 2442 3094 3271 3700 3926 4078 6187
Size: 900 49 652 177 429 226 152 2109
Cuts at: 6187 1493
Size: 1697
Fragments arranged by size:
2109 1697 900 652 429 226 177 152
49
AseI AT'TA_AT
Cuts at: 160 1032 4945 160
Size: 872 3913 1606
Fragments arranged by size:
3913 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6391
AvaI C'yCGr_G
Cuts at: 1870 1943 3716 1870
Size: 73 1773 4545
Fragments arranged by size:
4545 1773 73
BamHI G'GATC_C
Cuts at: 3721 4088 3721
Size: 367 6024
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2729 3712 5167 618
Size: 325 217 627 942 983 1455 1842
Fragments arranged by size:
1842 1455 983 942 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2372 3710 1391
Size: 981 1338 4072
Fragments arranged by size:
4072 1338 981
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3499 928
Size: 971 1600 3820
Fragments arranged by size:
3820 1600 971
BbvCI CC'TCA_GC
Cuts at: 3309 3309
Size: 6391
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2050 2084 4582 4616 1903
Size: 34 113 34 2498 34 3678
Fragments arranged by size:
3678 2498 113 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2311 4283 5493 2311
Size: 1972 1210 3209
Fragments arranged by size:
3209 1972 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6391
BfrBI ATG'CAT
Cuts at: 2680 2680
Size: 6391
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5002 136
Size: 107 122 71 631 605 3330 1525
Fragments arranged by size:
3330 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2661 6386 2661
Size: 3725 2666
BlpI GC'TnA_GC
Cuts at: 3808 3808
Size: 6391
Bme1580I G_kGCm'C
Cuts at: 1790 2060 2372 2626 2734 4452 5608 1790
Size: 270 312 254 108 1718 1156 2573
Fragments arranged by size:
2573 1718 1156 312 270 254 108
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6391
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2991 3434 3534
Size: 416 53 83 399 24 1574 443 100
Cuts at: 3534 4122 5080 442
Size: 588 958 1753
Fragments arranged by size:
1753 1574 958 588 443 416 399 100
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6391
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3039 3039
Size: 6391
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2406 2786 5051 1239
Size: 1167 380 2265 2579
Fragments arranged by size:
2579 2265 1167 380
Bpu10I CC'TnA_GC
Cuts at: 3309 3309
Size: 6391
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4516 5381 5679 4516
Size: 865 298 5228
Fragments arranged by size:
5228 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2819 3059 5054 882
Size: 617 1320 240 1995 2219
Fragments arranged by size:
2219 1995 1320 617 240
BsaAI yAC'GTr
Cuts at: 493 1204 4182 493
Size: 711 2978 2702
Fragments arranged by size:
2978 2702 711
BsaBI GATnn'nnATC
Cuts at: 2877 6385 2877
Size: 3508 2883
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4198 4580 5215 275
Size: 53 83 186 936 2665 382 635 1451
Fragments arranged by size:
2665 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3146 4824 5222 5496 5643 6054 3146
Size: 1678 398 274 147 411 3483
Fragments arranged by size:
3483 1678 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6166
Fragments arranged by size:
6166 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3146 3311 1186
Size: 1960 165 4266
Fragments arranged by size:
4266 1960 165
BseYI C'CCAG_C
Cuts at: 3645 5594 6090 3645
Size: 1949 496 3946
Fragments arranged by size:
3946 1949 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3657 1163
Size: 573 1921 3897
Fragments arranged by size:
3897 1921 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2194 2257 3841 4602 5630 1540
Size: 275 186 193 63 1584 761 1028 2301
Fragments arranged by size:
2301 1584 1028 761 275 193 186 63
BsiHKAI G_wGCw'C
Cuts at: 2626 3025 4452 4537 5608 2626
Size: 399 1427 85 1071 3409
Fragments arranged by size:
3409 1427 1071 399 85
BsmI GAATG_Cn'
Cuts at: 2961 3902 3995 2961
Size: 941 93 5357
Fragments arranged by size:
5357 941 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2372 2626 2734 3025 3710 4452
Size: 399 270 312 254 108 291 685 742
Cuts at: 4452 4537 5608 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 685 399 312 291 270
254 108 85
BspEI T'CCGG_A
Cuts at: 6054 6054
Size: 6391
BspHI T'CATG_A
Cuts at: 4280 6010 4280
Size: 1730 4661
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3746 844
Size: 2902 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2064 3519 4278 1394
Size: 429 241 1455 759 3507
Fragments arranged by size:
3507 1455 759 429 241
BsrDI GCAATG_nn'
Cuts at: 65 1248 2940 3627 4886 5060 65
Size: 1183 1692 687 1259 174 1396
Fragments arranged by size:
1692 1396 1259 1183 687 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2149 2193 3146 5035 5222 1700
Size: 110 339 44 953 1889 187 2869
Fragments arranged by size:
2869 1889 953 339 187 110 44
BsrGI T'GTAC_A
Cuts at: 96 3552 96
Size: 3456 2935
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6391
BssSI C'ACGA_G
Cuts at: 2059 3013 4451 5463 2059
Size: 954 1438 1012 2987
Fragments arranged by size:
2987 1438 1012 954
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6391
BstBI TT'CG_AA
Cuts at: 5195 5195
Size: 6391
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6391
BstXI CCAn_nnnn'nTGG
Cuts at: 2176 2981 3078 2176
Size: 805 97 5489
Fragments arranged by size:
5489 805 97
BstYI r'GATC_y
Cuts at: 1972 2661 2709 3721 4088 4473 4490 5942 6028
Size: 689 48 1012 367 385 17 1452 86
Cuts at: 6028 6040 6386 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1012 689 385 367 346 86
48 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2142 2989 513
Size: 552 939 138 847 3915
Fragments arranged by size:
3915 939 847 552 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2209 2218 487
Size: 683 56 983 9 4660
Fragments arranged by size:
4660 983 683 56 9
BtsI GCAGTG_nn'
Cuts at: 3669 3907 4701 4721 3669
Size: 238 794 20 5339
Fragments arranged by size:
5339 794 238 20
ClaI AT'CG_AT
Cuts at: 1554 3558 1554
Size: 2004 4387
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6356
DraI TTT'AAA
Cuts at: 3695 3695
Size: 6391
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2833 3602 5398 809
Size: 507 1517 769 1796 1802
Fragments arranged by size:
1802 1796 1517 769 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2191 3265 3838
Size: 54 1425 50 275 18 361 1074 573
Cuts at: 3838 4727 6356 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1074 889 573 361 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2191 3838 1537
Size: 275 379 1647 4090
Fragments arranged by size:
4090 1647 379 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4321 1353
Size: 2968 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4978 5352 5498 387
Size: 1064 138 3389 374 146 1280
Fragments arranged by size:
3389 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2406 2678 2786 2907 2941 4454 5051
Size: 393 774 272 108 121 34 1513 597
Cuts at: 5051 5838 6106 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1513 787 774 597 393 272 268
121 108 34
EcoO109I rG'GnC_Cy
Cuts at: 2368 2369 2414 3343 3595 2368
Size: 1 45 929 252 5164
Fragments arranged by size:
5164 929 252 45 1
EcoRI G'AATT_C
Cuts at: 3700 4078 3700
Size: 378 6013
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2519 2654 2519
Size: 135 6256
FspI TGC'GCA
Cuts at: 4897 4897
Size: 6391
HaeII r_GCGC'y
Cuts at: 1678 2103 5538 1678
Size: 425 3435 2531
Fragments arranged by size:
3435 2531 425
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2338 2370 2646 2678 3340
Size: 32 135 32 766 32 276 32 662
Cuts at: 3340 3372 5032 5064 5106 5138 1373
Size: 32 1660 32 42 32 2626
Fragments arranged by size:
2626 1660 766 662 276 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2752 3735 3981 669
Size: 988 281 814 983 246 3079
Fragments arranged by size:
3079 988 983 814 281 246
HindIII A'AGCT_T
Cuts at: 748 2583 748
Size: 1835 4556
HpaI GTT'AAC
Cuts at: 2752 3981 2752
Size: 1229 5162
KpnI G_GTAC'C
Cuts at: 3716 3716
Size: 6391
MfeI C'AATT_G
Cuts at: 2463 3990 2463
Size: 1527 4864
MluI A'CGCG_T
Cuts at: 6267 6267
Size: 6391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3535 5505 5689 1305
Size: 2230 1970 184 2007
Fragments arranged by size:
2230 2007 1970 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4569
Fragments arranged by size:
4569 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2174 3162 4349 4708 4867 518
Size: 648 1008 988 1187 359 159 2042
Fragments arranged by size:
2042 1187 1008 988 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2158 2813 2991 3649 3807 4484 5632 5877
Size: 552 655 178 658 158 677 1148 245
Cuts at: 5877 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 658 655 552 245 178
158
NaeI GCC'GGC
Cuts at: 2151 2151
Size: 6391
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4900
Fragments arranged by size:
4900 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6391
NgoMIV G'CCGG_C
Cuts at: 2149 2149
Size: 6391
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6391
NotI GC'GGCC_GC
Cuts at: 3838 3838
Size: 6391
NsiI A_TGCA'T
Cuts at: 2682 2682
Size: 6391
NspI r_CATG'y
Cuts at: 1132 1229 3010 3749 5294 1132
Size: 97 1781 739 1545 2229
Fragments arranged by size:
2229 1781 1545 739 97
PasI CC'CwG_GG
Cuts at: 1348 2363 1348
Size: 1015 5376
PciI A'CATG_T
Cuts at: 5290 5290
Size: 6391
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3446 1108
Size: 2338 4053
PmeI GTTT'AAAC
Cuts at: 3695 3695
Size: 6391
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4460 4492 5973 6005 4460
Size: 32 1481 32 4846
Fragments arranged by size:
4846 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2414 3595 2414
Size: 1181 5210
PshAI GACnn'nnGTC
Cuts at: 1613 2984 1613
Size: 1371 5020
PsiI TTA'TAA
Cuts at: 3961 3961
Size: 6391
PspOMI G'GGCC_C
Cuts at: 2368 2368
Size: 6391
PstI C_TGCA'G
Cuts at: 830 3654 3743 830
Size: 2824 89 3478
Fragments arranged by size:
3478 2824 89
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6391
PvuII CAG'CTG
Cuts at: 2813 3649 2813
Size: 836 5555
SacII CC_GC'GG
Cuts at: 2992 2992
Size: 6391
SalI G'TCGA_C
Cuts at: 1936 3733 1936
Size: 1797 4594
SbfI CC_TGCA'GG
Cuts at: 3743 3743
Size: 6391
ScaI AGT'ACT
Cuts at: 4639 6351 4639
Size: 1712 4679
SexAI A'CCwGG_T
Cuts at: 2817 3281 2817
Size: 464 5927
SfcI C'TryA_G
Cuts at: 826 1045 3650 3739 4874 5555 5746 826
Size: 219 2605 89 1135 681 191 1471
Fragments arranged by size:
2605 1471 1135 681 219 191 89
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6391
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6391
SmaI CCC'GGG
Cuts at: 1872 3718 1872
Size: 1846 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4495 5396 5658 820
Size: 197 926 2552 901 262 1553
Fragments arranged by size:
2552 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4182 493
Size: 3689 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6391
SphI G_CATG'C
Cuts at: 1132 3010 3749 1132
Size: 1878 739 3774
Fragments arranged by size:
3774 1878 739
SspI AAT'ATT
Cuts at: 5 52 2401 4315 5
Size: 47 2349 1914 2081
Fragments arranged by size:
2349 2081 1914 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6391
StyI C'CwwG_G
Cuts at: 513 1065 2004 2503 3830 513
Size: 552 939 499 1327 3074
Fragments arranged by size:
3074 1327 939 552 499
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2243 3691 4418 4435 4588 4773 5218
Size: 1255 663 1448 727 17 153 185 445
Cuts at: 5218 325
Size: 1498
Fragments arranged by size:
1498 1448 1255 727 663 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3552 4637 6349
Size: 274 80 33 51 1443 1575 1085 1712
Cuts at: 6349 96
Size: 138
Fragments arranged by size:
1712 1575 1443 1085 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6351 6383 6351
Size: 32 6359
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6391
XbaI T'CTAG_A
Cuts at: 3727 3727
Size: 6391
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2176 2176
Size: 6391
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6391
XmaI C'CCGG_G
Cuts at: 1870 3716 1870
Size: 1846 4545
XmnI GAAnn'nnTTC
Cuts at: 2087 3275 3377 3454 4520 2087
Size: 1188 102 77 1066 3958
Fragments arranged by size:
3958 1188 1066 102 77
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4199 276
Size: 53 83 186 936 2665 2468
Fragments arranged by size:
2665 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI
KasI NarI NruI PacI PfoI PmlI PspXI PsrI
RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI