Restriction map for
pFN21AE4521
(Circular) (Six-base) MAPSORT of: pFN21AE4521.seq Check: 9115 from: 1 to: 6376
pFN21AE4521 6376 bp
With 182 enzymes: SgfI *
March 28, 2012 14:08 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2373 4186 278
Size: 53 83 186 936 837 1813 2468
Fragments arranged by size:
2468 1813 936 837 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2373 3342 3719 1937
Size: 436 969 377 4594
Fragments arranged by size:
4594 969 436 377
Acc65I G'GTAC_C
Cuts at: 3262 3545 3697 3262
Size: 283 152 5941
Fragments arranged by size:
5941 283 152
AclI AA'CG_TT
Cuts at: 1563 4503 4876 1563
Size: 2940 373 3063
Fragments arranged by size:
3063 2940 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2536 4439 5823 6091 1632
Size: 904 1903 1384 268 1917
Fragments arranged by size:
1917 1903 1384 904 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6179
AflIII A'CryG_T
Cuts at: 1203 3345 5275 6252 1203
Size: 2142 1930 977 1327
Fragments arranged by size:
2142 1930 1327 977
AgeI A'CCGG_T
Cuts at: 5207 5207
Size: 6376
AhdI GACnn_n'nnGTC
Cuts at: 5105 5105
Size: 6376
AleI CACnn'nnGTG
Cuts at: 1166 2387 2824 3374 1166
Size: 1221 437 550 4168
Fragments arranged by size:
4168 1221 550 437
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6376
AlwNI CAG_nnn'CTG
Cuts at: 2187 4119 5691 2187
Size: 1932 1572 2872
Fragments arranged by size:
2872 1932 1572
ApaI G_GGCC'C
Cuts at: 2052 2052
Size: 6376
ApaLI G'TGCA_C
Cuts at: 4433 5589 4433
Size: 1156 5220
ApoI r'AATT_y
Cuts at: 1493 2578 2664 3685 3911 4063 6172 1493
Size: 1085 86 1021 226 152 2109 1697
Fragments arranged by size:
2109 1697 1085 1021 226 152 86
AseI AT'TA_AT
Cuts at: 160 1032 4930 160
Size: 872 3898 1606
Fragments arranged by size:
3898 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
AvaI C'yCGr_G
Cuts at: 1870 1943 2947 3701 1870
Size: 73 1004 754 4545
Fragments arranged by size:
4545 1004 754 73
BamHI G'GATC_C
Cuts at: 3706 4073 3706
Size: 367 6009
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2158 2233 3262 3455 3545
Size: 325 217 627 371 75 1029 193 90
Cuts at: 3545 3697 5152 618
Size: 152 1455 1842
Fragments arranged by size:
1842 1455 1029 627 371 325 217 193
152 90 75
BanII G_rGCy'C
Cuts at: 1391 2052 2474 3695 1391
Size: 661 422 1221 4072
Fragments arranged by size:
4072 1221 661 422
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5405
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3655 3689 4567 4601 1903
Size: 34 50 34 1634 34 878 34 3678
Fragments arranged by size:
3678 1634 878 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3375 4268 5478 3375
Size: 893 1210 4273
Fragments arranged by size:
4273 1210 893
BclI T'GATC_A
Cuts at: 1548 2889 1548
Size: 1341 5035
BfrBI ATG'CAT
Cuts at: 2723 2723
Size: 6376
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4987 136
Size: 107 122 71 631 605 3315 1525
Fragments arranged by size:
3315 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2565 2625 6371 2565
Size: 60 3746 2570
Fragments arranged by size:
3746 2570 60
BlpI GC'TnA_GC
Cuts at: 2078 3793 2078
Size: 1715 4661
Bme1580I G_kGCm'C
Cuts at: 1790 2052 2238 4437 5593 1790
Size: 262 186 2199 1156 2573
Fragments arranged by size:
2573 2199 1156 262 186
BmgBI CAC'GTC
Cuts at: 1318 2311 1318
Size: 993 5383
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4107 5065 442
Size: 416 53 83 399 24 2690 958 1753
Fragments arranged by size:
2690 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6376
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2315 2348 5036 1239
Size: 1076 33 2688 2579
Fragments arranged by size:
2688 2579 1076 33
Bpu10I CC'TnA_GC
Cuts at: 2177 2177
Size: 6376
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4501 5366 5664 4501
Size: 865 298 5213
Fragments arranged by size:
5213 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5039 882
Size: 617 3540 2219
Fragments arranged by size:
3540 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3346 4167 493
Size: 711 2142 821 2702
Fragments arranged by size:
2702 2142 821 711
BsaBI GATnn'nnATC
Cuts at: 6370 6370
Size: 6376
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2166 2370 4183 4565
Size: 53 83 186 936 633 204 1813 382
Cuts at: 4565 5200 275
Size: 635 1451
Fragments arranged by size:
1813 1451 936 635 633 382 204 186
83 53
BsaWI w'CCGG_w
Cuts at: 2344 3024 4809 5207 5481 5628 6039 2344
Size: 680 1785 398 274 147 411 2681
Fragments arranged by size:
2681 1785 680 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6151
Fragments arranged by size:
6151 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6376
BseYI C'CCAG_C
Cuts at: 2352 3157 5579 6075 2352
Size: 805 2422 496 2653
Fragments arranged by size:
2653 2422 805 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5803
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2227 3466 3826 4587 5615 1540
Size: 275 186 226 1239 360 761 1028 2301
Fragments arranged by size:
2301 1239 1028 761 360 275 226 186
BsiHKAI G_wGCw'C
Cuts at: 2148 4437 4522 5593 2148
Size: 2289 85 1071 2931
Fragments arranged by size:
2931 2289 1071 85
BsmI GAATG_Cn'
Cuts at: 3887 3980 3887
Size: 93 6283
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2148 2238 2474 3695 4437 4522
Size: 399 262 96 90 236 1221 742 85
Cuts at: 4522 5593 1391
Size: 1071 2174
Fragments arranged by size:
2174 1221 1071 742 399 262 236 96
90 85
BspEI T'CCGG_A
Cuts at: 2344 3024 6039 2344
Size: 680 3015 2681
Fragments arranged by size:
3015 2681 680
BspHI T'CATG_A
Cuts at: 4265 5995 4265
Size: 1730 4646
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3731 844
Size: 2887 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4263 1394
Size: 429 2440 3507
Fragments arranged by size:
3507 2440 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4871 5045 65
Size: 1183 3623 174 1396
Fragments arranged by size:
3623 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2108 2256 5020 5207 1700
Size: 110 298 148 2764 187 2869
Fragments arranged by size:
2869 2764 298 187 148 110
BsrGI T'GTAC_A
Cuts at: 96 3348 96
Size: 3252 3124
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6376
BssSI C'ACGA_G
Cuts at: 4436 5448 4436
Size: 1012 5364
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3193 794
Size: 2399 3977
BstBI TT'CG_AA
Cuts at: 3010 5180 3010
Size: 2170 4206
BstEII G'GTnAC_C
Cuts at: 1184 2023 3031 1184
Size: 839 1008 4529
Fragments arranged by size:
4529 1008 839
BstXI CCAn_nnnn'nTGG
Cuts at: 2638 3070 2638
Size: 432 5944
BstYI r'GATC_y
Cuts at: 1972 2565 2625 3001 3706 4073 4458 4475 5927
Size: 593 60 376 705 367 385 17 1452
Cuts at: 5927 6013 6025 6371 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 705 593 385 376 367 346
86 60 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2532 3210 3336 513
Size: 552 1467 678 126 3553
Fragments arranged by size:
3553 1467 678 552 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2254 3158 487
Size: 683 56 1028 904 3705
Fragments arranged by size:
3705 1028 904 683 56
BtsI GCAGTG_nn'
Cuts at: 2207 3216 3244 3892 4686 4706 2207
Size: 1009 28 648 794 20 3877
Fragments arranged by size:
3877 1009 794 648 28 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6376
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2923 2958 529
Size: 35 2359 35 3947
Fragments arranged by size:
3947 2359 35 35
DraI TTT'AAA
Cuts at: 3680 3680
Size: 6376
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5383 809
Size: 507 4067 1802
Fragments arranged by size:
4067 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2224 2506 3213
Size: 54 1425 50 275 18 394 282 707
Cuts at: 3213 3823 4712 6341 8
Size: 610 889 1629 43
Fragments arranged by size:
1629 1425 889 707 610 394 282 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2224 3823 1537
Size: 275 412 1599 4090
Fragments arranged by size:
4090 1599 412 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2871 3230 3254 4306 1353
Size: 1518 359 24 1052 3423
Fragments arranged by size:
3423 1518 1052 359 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2788 4963 5337 5483 387
Size: 1064 138 1199 2175 374 146 1280
Fragments arranged by size:
2175 1280 1199 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2315 2348 2536 4439 5036 5823 6091
Size: 393 683 33 188 1903 597 787 268
Cuts at: 6091 1239
Size: 1524
Fragments arranged by size:
1903 1524 787 683 597 393 268 188
33
EcoO109I rG'GnC_Cy
Cuts at: 2048 2049 2428 2048
Size: 1 379 5996
Fragments arranged by size:
5996 379 1
EcoRI G'AATT_C
Cuts at: 3685 4063 3685
Size: 378 5998
FspI TGC'GCA
Cuts at: 4882 4882
Size: 6376
HaeII r_GCGC'y
Cuts at: 1678 2062 2085 2187 2288 2792 3205 5523 1678
Size: 384 23 102 101 504 413 2318 2531
Fragments arranged by size:
2531 2318 504 413 384 102 101 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2841 2873 5017 5049 5091
Size: 32 135 32 1269 32 2144 32 42
Cuts at: 5091 5123 1373
Size: 32 2626
Fragments arranged by size:
2626 2144 1269 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3720 3966 669
Size: 988 281 1782 246 3079
Fragments arranged by size:
3079 1782 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6376
HpaI GTT'AAC
Cuts at: 3966 3966
Size: 6376
KpnI G_GTAC'C
Cuts at: 3266 3549 3701 3266
Size: 283 152 5941
Fragments arranged by size:
5941 283 152
MfeI C'AATT_G
Cuts at: 3975 3975
Size: 6376
MluI A'CGCG_T
Cuts at: 6252 6252
Size: 6376
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2655 5490 5674 1305
Size: 1350 2835 184 2007
Fragments arranged by size:
2835 2007 1350 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3215 10
Size: 54 1425 343 1383 3171
Fragments arranged by size:
3171 1425 1383 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2387 2537 2824 3038 3068 3122 3374
Size: 648 1221 150 287 214 30 54 252
Cuts at: 3374 3389 3485 3623 4334 4693 4852 518
Size: 15 96 138 711 359 159 2042
Fragments arranged by size:
2042 1221 711 648 359 287 252 214
159 150 138 96 54 30 15
MspA1I CmG'CkG
Cuts at: 1606 2151 2244 2326 2491 3259 3792 4469 5617
Size: 545 93 82 165 768 533 677 1148
Cuts at: 5617 5862 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 768 677 545 533 245 165
93 82
NcoI C'CATG_G
Cuts at: 513 1065 2532 3210 3336 513
Size: 552 1467 678 126 3553
Fragments arranged by size:
3553 1467 678 552 126
NdeI CA'TA_TG
Cuts at: 387 3163 387
Size: 2776 3600
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6376
NotI GC'GGCC_GC
Cuts at: 3823 3823
Size: 6376
NsiI A_TGCA'T
Cuts at: 2725 2725
Size: 6376
NspI r_CATG'y
Cuts at: 1132 1229 2542 3734 5279 1132
Size: 97 1313 1192 1545 2229
Fragments arranged by size:
2229 1545 1313 1192 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6376
PciI A'CATG_T
Cuts at: 5275 5275
Size: 6376
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6376
PmeI GTTT'AAAC
Cuts at: 3680 3680
Size: 6376
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4445 4477 5958 5990 4445
Size: 32 1481 32 4831
Fragments arranged by size:
4831 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6376
PsiI TTA'TAA
Cuts at: 3946 3946
Size: 6376
PspOMI G'GGCC_C
Cuts at: 2048 2048
Size: 6376
PstI C_TGCA'G
Cuts at: 830 2565 3728 830
Size: 1735 1163 3478
Fragments arranged by size:
3478 1735 1163
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6376
PvuII CAG'CTG
Cuts at: 2244 2326 2491 3259 2244
Size: 82 165 768 5361
Fragments arranged by size:
5361 768 165 82
SalI G'TCGA_C
Cuts at: 1936 3718 1936
Size: 1782 4594
SbfI CC_TGCA'GG
Cuts at: 3728 3728
Size: 6376
ScaI AGT'ACT
Cuts at: 4624 6336 4624
Size: 1712 4664
SexAI A'CCwGG_T
Cuts at: 2403 2403
Size: 6376
SfcI C'TryA_G
Cuts at: 826 1045 2561 3288 3724 4859 5540 5731 826
Size: 219 1516 727 436 1135 681 191 1471
Fragments arranged by size:
1516 1471 1135 727 681 436 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6376
SmaI CCC'GGG
Cuts at: 1872 3703 1872
Size: 1831 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4480 5381 5643 820
Size: 197 926 2537 901 262 1553
Fragments arranged by size:
2537 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4167 493
Size: 3674 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6376
SphI G_CATG'C
Cuts at: 1132 2542 3734 1132
Size: 1410 1192 3774
Fragments arranged by size:
3774 1410 1192
SspI AAT'ATT
Cuts at: 5 52 2691 3191 4300 5
Size: 47 2639 500 1109 2081
Fragments arranged by size:
2639 2081 1109 500 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6376
StyI C'CwwG_G
Cuts at: 513 1065 2532 3210 3336 3451 3815 513
Size: 552 1467 678 126 115 364 3074
Fragments arranged by size:
3074 1467 678 552 364 126 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2408 2447 3221 3377 4403 4420 4573
Size: 1255 828 39 774 156 1026 17 153
Cuts at: 4573 4758 5203 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1026 828 774 445 185 156
153 39 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3348 4622 6334
Size: 274 80 33 51 1443 1371 1274 1712
Cuts at: 6334 96
Size: 138
Fragments arranged by size:
1712 1443 1371 1274 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6336 6368 6336
Size: 32 6344
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6376
XbaI T'CTAG_A
Cuts at: 3712 3712
Size: 6376
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6376
XmaI C'CCGG_G
Cuts at: 1870 3701 1870
Size: 1831 4545
XmnI GAAnn'nnTTC
Cuts at: 3131 4505 3131
Size: 1374 5002
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2371 4184 276
Size: 53 83 186 936 837 1813 2468
Fragments arranged by size:
2468 1813 936 837 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI