Restriction map for
pFN21AE4518
(Circular) (Six-base) MAPSORT of: pFN21AE4518.seq Check: 6436 from: 1 to: 6361
pFN21AE4518 6361 bp
With 182 enzymes: SgfI *
March 28, 2012 14:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2248 3011 2248
Size: 763 5598
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2220 4171 278
Size: 53 83 186 936 684 1951 2468
Fragments arranged by size:
2468 1951 936 684 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2205 3704 1937
Size: 268 1499 4594
Fragments arranged by size:
4594 1499 268
Acc65I G'GTAC_C
Cuts at: 3682 3682
Size: 6361
AclI AA'CG_TT
Cuts at: 1563 4488 4861 1563
Size: 2925 373 3063
Fragments arranged by size:
3063 2925 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2261 2489 4424 5808 6076 1632
Size: 629 228 1935 1384 268 1917
Fragments arranged by size:
1935 1917 1384 629 268 228
AflII C'TTAA_G
Cuts at: 820 1017 2126 820
Size: 197 1109 5055
Fragments arranged by size:
5055 1109 197
AflIII A'CryG_T
Cuts at: 1203 2166 2280 5260 6237 1203
Size: 963 114 2980 977 1327
Fragments arranged by size:
2980 1327 977 963 114
AgeI A'CCGG_T
Cuts at: 5192 5192
Size: 6361
AhdI GACnn_n'nnGTC
Cuts at: 2335 5090 2335
Size: 2755 3606
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6361
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6361
AlwNI CAG_nnn'CTG
Cuts at: 3003 4104 5676 3003
Size: 1101 1572 3688
Fragments arranged by size:
3688 1572 1101
ApaI G_GGCC'C
Cuts at: 2277 2277
Size: 6361
ApaLI G'TGCA_C
Cuts at: 4418 5574 4418
Size: 1156 5205
ApoI r'AATT_y
Cuts at: 1493 2985 3670 3896 4048 6157 1493
Size: 1492 685 226 152 2109 1697
Fragments arranged by size:
2109 1697 1492 685 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4915 160
Size: 872 3883 1606
Fragments arranged by size:
3883 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6361
AvaI C'yCGr_G
Cuts at: 1870 1943 2345 3429 3686 1870
Size: 73 402 1084 257 4545
Fragments arranged by size:
4545 1084 402 257 73
BamHI G'GATC_C
Cuts at: 2694 3691 4058 2694
Size: 997 367 4997
Fragments arranged by size:
4997 997 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2098 3682 5137 618
Size: 325 217 627 311 1584 1455 1842
Fragments arranged by size:
1842 1584 1455 627 325 311 217
BanII G_rGCy'C
Cuts at: 1391 2277 2637 2658 3509 3680 1391
Size: 886 360 21 851 171 4072
Fragments arranged by size:
4072 886 851 360 171 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5390
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4552 4586 1903
Size: 34 2615 34 3678
Fragments arranged by size:
3678 2615 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4253 5463 4253
Size: 1210 5151
BclI T'GATC_A
Cuts at: 1548 2763 1548
Size: 1215 5146
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4972 136
Size: 107 122 71 631 605 3300 1525
Fragments arranged by size:
3300 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6356 6356
Size: 6361
BlpI GC'TnA_GC
Cuts at: 2631 2723 3173 3778 2631
Size: 92 450 605 5214
Fragments arranged by size:
5214 605 450 92
Bme1580I G_kGCm'C
Cuts at: 1790 2277 3019 3653 4422 5578 1790
Size: 487 742 634 769 1156 2573
Fragments arranged by size:
2573 1156 769 742 634 487
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6361
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2142 3222 3331
Size: 416 53 83 399 24 725 1080 109
Cuts at: 3331 4092 5050 442
Size: 761 958 1753
Fragments arranged by size:
1753 1080 958 761 725 416 399 109
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6361
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3124 3528 3124
Size: 404 5957
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2840 2996 3498 3523 5021 1239
Size: 1601 156 502 25 1498 2579
Fragments arranged by size:
2579 1601 1498 502 156 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4486 5351 5649 4486
Size: 865 298 5198
Fragments arranged by size:
5198 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5024 882
Size: 617 3525 2219
Fragments arranged by size:
3525 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2281 4152 493
Size: 711 1077 1871 2702
Fragments arranged by size:
2702 1871 1077 711
BsaBI GATnn'nnATC
Cuts at: 2183 6355 2183
Size: 4172 2189
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2049 2217 4168 4550
Size: 53 83 186 936 516 168 1951 382
Cuts at: 4550 5185 275
Size: 635 1451
Fragments arranged by size:
1951 1451 936 635 516 382 186 168
83 53
BsaWI w'CCGG_w
Cuts at: 3396 4794 5192 5466 5613 6024 3396
Size: 1398 398 274 147 411 3733
Fragments arranged by size:
3733 1398 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6136
Fragments arranged by size:
6136 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6361
BseYI C'CCAG_C
Cuts at: 2028 2044 2074 2228 3355 3456 3558 5564 6060
Size: 16 30 154 1127 101 102 2006 496
Cuts at: 6060 2028
Size: 2329
Fragments arranged by size:
2329 2006 1127 496 154 102 101 30
16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3071 1163
Size: 573 1335 4453
Fragments arranged by size:
4453 1335 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3811 4572 5600 1540
Size: 275 186 1810 761 1028 2301
Fragments arranged by size:
2301 1810 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2637 4422 4507 5578 2637
Size: 1785 85 1071 3420
Fragments arranged by size:
3420 1785 1071 85
BsmI GAATG_Cn'
Cuts at: 2489 2743 3872 3965 2489
Size: 254 1129 93 4885
Fragments arranged by size:
4885 1129 254 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3536 3536
Size: 6361
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2277 2637 2658 3019 3509 3653 3680
Size: 399 487 360 21 361 490 144 27
Cuts at: 3680 4422 4507 5578 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 490 487 399 361 360
144 85 27 21
BspEI T'CCGG_A
Cuts at: 3396 6024 3396
Size: 2628 3733
BspHI T'CATG_A
Cuts at: 2085 2220 2478 2517 2799 4250 5980 2085
Size: 135 258 39 282 1451 1730 2466
Fragments arranged by size:
2466 1730 1451 282 258 135 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2110 2248 2997 3011 3716 844
Size: 1266 138 749 14 705 3489
Fragments arranged by size:
3489 1266 749 705 138 14
BsrBI CCG'CTC
Cuts at: 1394 1823 3261 3549 4248 1394
Size: 429 1438 288 699 3507
Fragments arranged by size:
3507 1438 699 429 288
BsrDI GCAATG_nn'
Cuts at: 65 1248 4856 5030 65
Size: 1183 3608 174 1396
Fragments arranged by size:
3608 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5005 5192 1700
Size: 110 3195 187 2869
Fragments arranged by size:
3195 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2283 2862 96
Size: 2187 579 3595
Fragments arranged by size:
3595 2187 579
BssHII G'CGCG_C
Cuts at: 1925 2024 1925
Size: 99 6262
BssSI C'ACGA_G
Cuts at: 2702 4421 5433 2702
Size: 1719 1012 3630
Fragments arranged by size:
3630 1719 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6361
BstBI TT'CG_AA
Cuts at: 5165 5165
Size: 6361
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6361
BstXI CCAn_nnnn'nTGG
Cuts at: 2422 2422
Size: 6361
BstYI r'GATC_y
Cuts at: 1972 2178 2235 2694 3691 4058 4443 4460 5912
Size: 206 57 459 997 367 385 17 1452
Cuts at: 5912 5998 6010 6356 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 997 459 385 367 346 206
86 57 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2383 3267 513
Size: 552 939 379 884 3607
Fragments arranged by size:
3607 939 884 552 379
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5622
Fragments arranged by size:
5622 683 56
BtsI GCAGTG_nn'
Cuts at: 3553 3877 4671 4691 3553
Size: 324 794 20 5223
Fragments arranged by size:
5223 794 324 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6361
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6326
DraI TTT'AAA
Cuts at: 3665 3665
Size: 6361
DraIII CAC_nnn'GTG
Cuts at: 2704 2704
Size: 6361
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2405 2809 5368 809
Size: 507 1089 404 2559 1802
Fragments arranged by size:
2559 1802 1089 507 404
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2789 3415 3488
Size: 54 1425 50 275 18 959 626 73
Cuts at: 3488 3808 4697 6326 8
Size: 320 889 1629 43
Fragments arranged by size:
1629 1425 959 889 626 320 275 73
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3808 1537
Size: 275 1996 4090
Fragments arranged by size:
4090 1996 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2663 3288 4291 1353
Size: 1310 625 1003 3423
Fragments arranged by size:
3423 1310 1003 625
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2005 4948 5322 5468 387
Size: 1064 138 416 2943 374 146 1280
Fragments arranged by size:
2943 1280 1064 416 374 146 138
EcoICRI GAG'CTC
Cuts at: 2635 2635
Size: 6361
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2261 2489 2840 2996 3498 3523 4424
Size: 393 629 228 351 156 502 25 901
Cuts at: 4424 5021 5808 6076 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 901 787 629 597 502 393 351
268 228 156 25
EcoO109I rG'GnC_Cy
Cuts at: 2122 2274 2557 3468 3632 2122
Size: 152 283 911 164 4851
Fragments arranged by size:
4851 911 283 164 152
EcoRI G'AATT_C
Cuts at: 2985 3670 4048 2985
Size: 685 378 5298
Fragments arranged by size:
5298 685 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2752 2752
Size: 6361
FspI TGC'GCA
Cuts at: 2138 3569 4867 2138
Size: 1431 1298 3632
Fragments arranged by size:
3632 1431 1298
FspAI rTGC'GCAy
Cuts at: 2138 2138
Size: 6361
HaeII r_GCGC'y
Cuts at: 1678 2045 2082 3356 5508 1678
Size: 367 37 1274 2152 2531
Fragments arranged by size:
2531 2152 1274 367 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2321 2353 5002 5034 5076
Size: 32 135 32 749 32 2649 32 42
Cuts at: 5076 5108 1373
Size: 32 2626
Fragments arranged by size:
2649 2626 749 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2734 3705 3951 669
Size: 988 281 796 971 246 3079
Fragments arranged by size:
3079 988 971 796 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6361
HpaI GTT'AAC
Cuts at: 3951 3951
Size: 6361
KpnI G_GTAC'C
Cuts at: 3686 3686
Size: 6361
MfeI C'AATT_G
Cuts at: 3960 3960
Size: 6361
MluI A'CGCG_T
Cuts at: 6237 6237
Size: 6361
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2670 5475 5659 1305
Size: 1365 2805 184 2007
Fragments arranged by size:
2805 2007 1365 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3417 3490 10
Size: 54 1425 343 1585 73 2881
Fragments arranged by size:
2881 1585 1425 343 73 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2090 2408 4319 4678 4837 518
Size: 648 924 318 1911 359 159 2042
Fragments arranged by size:
2042 1911 924 648 359 318 159
MspA1I CmG'CkG
Cuts at: 1606 3172 3241 3304 3777 4454 5602 5847 1606
Size: 1566 69 63 473 677 1148 245 2120
Fragments arranged by size:
2120 1566 1148 677 473 245 69 63
NcoI C'CATG_G
Cuts at: 513 1065 2004 3267 513
Size: 552 939 1263 3607
Fragments arranged by size:
3607 1263 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6361
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6361
NotI GC'GGCC_GC
Cuts at: 3808 3808
Size: 6361
NspI r_CATG'y
Cuts at: 1132 1229 2170 2599 3594 3719 5264 1132
Size: 97 941 429 995 125 1545 2229
Fragments arranged by size:
2229 1545 995 941 429 125 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6361
PciI A'CATG_T
Cuts at: 2166 5260 2166
Size: 3094 3267
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2704 1108
Size: 1596 4765
PfoI T'CCnGG_A
Cuts at: 3117 3117
Size: 6361
PmeI GTTT'AAAC
Cuts at: 3665 3665
Size: 6361
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4430 4462 5943 5975 4430
Size: 32 1481 32 4816
Fragments arranged by size:
4816 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2122 2557 3632 2122
Size: 435 1075 4851
Fragments arranged by size:
4851 1075 435
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6361
PsiI TTA'TAA
Cuts at: 3931 3931
Size: 6361
PspOMI G'GGCC_C
Cuts at: 2273 2273
Size: 6361
PspXI vC'TCGA_Gb
Cuts at: 2345 2345
Size: 6361
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2276 2308 2276
Size: 32 6329
PstI C_TGCA'G
Cuts at: 830 3008 3171 3713 830
Size: 2178 163 542 3478
Fragments arranged by size:
3478 2178 542 163
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6361
PvuII CAG'CTG
Cuts at: 3172 3304 3172
Size: 132 6229
SacI G_AGCT'C
Cuts at: 2637 2637
Size: 6361
SalI G'TCGA_C
Cuts at: 1936 3703 1936
Size: 1767 4594
SanDI GG'GwC_CC
Cuts at: 3632 3632
Size: 6361
SapI GCTCTTCn'nnn_
Cuts at: 2663 2663
Size: 6361
SbfI CC_TGCA'GG
Cuts at: 3008 3713 3008
Size: 705 5656
ScaI AGT'ACT
Cuts at: 2585 4609 6321 2585
Size: 2024 1712 2625
Fragments arranged by size:
2625 2024 1712
SfcI C'TryA_G
Cuts at: 826 1045 2827 3004 3167 3709 4844 5525 5716
Size: 219 1782 177 163 542 1135 681 191
Cuts at: 5716 826
Size: 1471
Fragments arranged by size:
1782 1471 1135 681 542 219 191 177
163
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6361
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6361
SmaI CCC'GGG
Cuts at: 1872 3431 3688 1872
Size: 1559 257 4545
Fragments arranged by size:
4545 1559 257
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2126 2345 4465 5366 5628 820
Size: 197 926 183 219 2120 901 262 1553
Fragments arranged by size:
2120 1553 926 901 262 219 197 183
SnaBI TAC'GTA
Cuts at: 493 4152 493
Size: 3659 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6361
SphI G_CATG'C
Cuts at: 1132 3594 3719 1132
Size: 2462 125 3774
Fragments arranged by size:
3774 2462 125
SrfI GCCC'GGGC
Cuts at: 3431 3431
Size: 6361
SspI AAT'ATT
Cuts at: 5 52 4285 5
Size: 47 4233 2081
Fragments arranged by size:
4233 2081 47
StuI AGG'CCT
Cuts at: 1517 2840 3627 1517
Size: 1323 787 4251
Fragments arranged by size:
4251 1323 787
StyI C'CwwG_G
Cuts at: 513 1065 2004 2484 3267 3318 3640 3800 513
Size: 552 939 480 783 51 322 160 3074
Fragments arranged by size:
3074 939 783 552 480 322 160 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2420 2522 2965 4388 4405 4558 4743
Size: 1255 840 102 443 1423 17 153 185
Cuts at: 4743 5188 325
Size: 445 1498
Fragments arranged by size:
1498 1423 1255 840 445 443 185 153
102 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2283 2583 2862
Size: 274 80 33 51 1443 306 300 279
Cuts at: 2862 4607 6319 96
Size: 1745 1712 138
Fragments arranged by size:
1745 1712 1443 306 300 279 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6321 6353 6321
Size: 32 6329
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6361
XbaI T'CTAG_A
Cuts at: 3697 3697
Size: 6361
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3410 3498 3410
Size: 88 6273
XhoI C'TCGA_G
Cuts at: 1943 2345 1943
Size: 402 5959
XmaI C'CCGG_G
Cuts at: 1870 3429 3686 1870
Size: 1559 257 4545
Fragments arranged by size:
4545 1559 257
XmnI GAAnn'nnTTC
Cuts at: 4490 4490
Size: 6361
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2218 4169 276
Size: 53 83 186 936 684 1951 2468
Fragments arranged by size:
2468 1951 936 684 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI RsrII
SacII SexAI SfiI SfoI SwaI