Restriction map for
pFN21AE4517
(Circular) (Six-base) MAPSORT of: pFN21AE4517.seq Check: 7787 from: 1 to: 6355
pFN21AE4517 6355 bp
With 182 enzymes: SgfI *
March 28, 2012 14:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2053 2053
Size: 6355
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4165 278
Size: 53 83 186 936 2629 2468
Fragments arranged by size:
2629 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3698 1937
Size: 1761 4594
Acc65I G'GTAC_C
Cuts at: 3676 3676
Size: 6355
AclI AA'CG_TT
Cuts at: 1563 4482 4855 1563
Size: 2919 373 3063
Fragments arranged by size:
3063 2919 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2701 2867 4418 5802 6070 1632
Size: 1069 166 1551 1384 268 1917
Fragments arranged by size:
1917 1551 1384 1069 268 166
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6158
AflIII A'CryG_T
Cuts at: 1203 5254 6231 1203
Size: 4051 977 1327
Fragments arranged by size:
4051 1327 977
AgeI A'CCGG_T
Cuts at: 2499 5186 2499
Size: 2687 3668
AhdI GACnn_n'nnGTC
Cuts at: 5084 5084
Size: 6355
AleI CACnn'nnGTG
Cuts at: 1166 2387 1166
Size: 1221 5134
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6355
AlwNI CAG_nnn'CTG
Cuts at: 2059 3112 3289 3583 4098 5670 2059
Size: 1053 177 294 515 1572 2744
Fragments arranged by size:
2744 1572 1053 515 294 177
ApaI G_GGCC'C
Cuts at: 2250 2250
Size: 6355
ApaLI G'TGCA_C
Cuts at: 4412 5568 4412
Size: 1156 5199
ApoI r'AATT_y
Cuts at: 1493 3061 3664 3890 4042 6151 1493
Size: 1568 603 226 152 2109 1697
Fragments arranged by size:
2109 1697 1568 603 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4909 160
Size: 872 3877 1606
Fragments arranged by size:
3877 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6355
AvaI C'yCGr_G
Cuts at: 1870 1943 3680 1870
Size: 73 1737 4545
Fragments arranged by size:
4545 1737 73
BamHI G'GATC_C
Cuts at: 2460 3685 4052 2460
Size: 1225 367 4763
Fragments arranged by size:
4763 1225 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2555 3268 3676 5131 618
Size: 325 217 627 768 713 408 1455 1842
Fragments arranged by size:
1842 1455 768 713 627 408 325 217
BanII G_rGCy'C
Cuts at: 1391 2250 2474 2892 3674 1391
Size: 859 224 418 782 4072
Fragments arranged by size:
4072 859 782 418 224
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2365 3088 928
Size: 971 466 723 4195
Fragments arranged by size:
4195 971 723 466
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2551 2585 4546 4580 1903
Size: 34 614 34 1961 34 3678
Fragments arranged by size:
3678 1961 614 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4247 5457 4247
Size: 1210 5145
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6355
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2281 4966 136
Size: 107 122 71 631 605 609 2685 1525
Fragments arranged by size:
2685 1525 631 609 605 122 107 71
BglII A'GATC_T
Cuts at: 6350 6350
Size: 6355
BlpI GC'TnA_GC
Cuts at: 2522 3772 2522
Size: 1250 5105
Bme1580I G_kGCm'C
Cuts at: 1790 2250 2558 2799 3024 4416 5572 1790
Size: 460 308 241 225 1392 1156 2573
Fragments arranged by size:
2573 1392 1156 460 308 241 225
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6355
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4086 5044 442
Size: 416 53 83 399 24 2669 958 1753
Fragments arranged by size:
2669 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6355
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2140 2995 2140
Size: 855 5500
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2135 2338 2762 2788 3055 3119 3605 5015
Size: 896 203 424 26 267 64 486 1410
Cuts at: 5015 1239
Size: 2579
Fragments arranged by size:
2579 1410 896 486 424 267 203 64
26
Bpu10I CC'TnA_GC
Cuts at: 2435 2435
Size: 6355
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4480 5345 5643 4480
Size: 865 298 5192
Fragments arranged by size:
5192 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3539 5018 882
Size: 617 2040 1479 2219
Fragments arranged by size:
2219 2040 1479 617
BsaAI yAC'GTr
Cuts at: 493 1204 4146 493
Size: 711 2942 2702
Fragments arranged by size:
2942 2702 711
BsaBI GATnn'nnATC
Cuts at: 6349 6349
Size: 6355
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4162 4544 5179 275
Size: 53 83 186 936 2629 382 635 1451
Fragments arranged by size:
2629 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2499 4788 5186 5460 5607 6018 2499
Size: 2289 398 274 147 411 2836
Fragments arranged by size:
2836 2289 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2981 3011 3546 3576 1178
Size: 30 165 30 1578 30 535 30 3957
Fragments arranged by size:
3957 1578 535 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2480 2984 3052 3122 3474 3479 1186
Size: 1294 504 68 70 352 5 4062
Fragments arranged by size:
4062 1294 504 352 70 68 5
BseYI C'CCAG_C
Cuts at: 2352 2737 3310 3424 5558 6054 2352
Size: 385 573 114 2134 496 2653
Fragments arranged by size:
2653 2134 573 496 385 114
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2431 1163
Size: 573 695 5087
Fragments arranged by size:
5087 695 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2999 3805 4566 5594 1540
Size: 275 186 998 806 761 1028 2301
Fragments arranged by size:
2301 1028 998 806 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2070 2892 3030 4416 4501 5572 2070
Size: 822 138 1386 85 1071 2853
Fragments arranged by size:
2853 1386 1071 822 138 85
BsmI GAATG_Cn'
Cuts at: 3402 3866 3959 3402
Size: 464 93 5798
Fragments arranged by size:
5798 464 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2250 2474 2558 2799 2892 3024
Size: 399 280 180 224 84 241 93 132
Cuts at: 3024 3030 3674 4416 4501 5572 1391
Size: 6 644 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 644 399 280 241 224
180 132 93 85 84 6
BspEI T'CCGG_A
Cuts at: 6018 6018
Size: 6355
BspHI T'CATG_A
Cuts at: 2091 4244 5974 2091
Size: 2153 1730 2472
Fragments arranged by size:
2472 2153 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2053 3111 3350 3710 844
Size: 1209 1058 239 360 3489
Fragments arranged by size:
3489 1209 1058 360 239
BsrBI CCG'CTC
Cuts at: 1394 1823 3562 4242 1394
Size: 429 1739 680 3507
Fragments arranged by size:
3507 1739 680 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3507 4850 5024 65
Size: 1183 2259 1343 174 1396
Fragments arranged by size:
2259 1396 1343 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2499 2627 3239 3420 3618 4999 5186
Size: 110 689 128 612 181 198 1381 187
Cuts at: 5186 1700
Size: 2869
Fragments arranged by size:
2869 1381 689 612 198 187 181 128
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6355
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6355
BssSI C'ACGA_G
Cuts at: 4415 5427 4415
Size: 1012 5343
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3280 3289 794
Size: 2486 9 3860
Fragments arranged by size:
3860 2486 9
BstBI TT'CG_AA
Cuts at: 5159 5159
Size: 6355
BstEII G'GTnAC_C
Cuts at: 1184 2689 1184
Size: 1505 4850
BstXI CCAn_nnnn'nTGG
Cuts at: 2389 2701 3055 3265 2389
Size: 312 354 210 5479
Fragments arranged by size:
5479 354 312 210
BstYI r'GATC_y
Cuts at: 1972 2460 3685 4052 4437 4454 5906 5992 6004
Size: 488 1225 367 385 17 1452 86 12
Cuts at: 6004 6350 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1225 488 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2304 2547 2993 3153 3234 513
Size: 552 939 300 243 446 160 81 3634
Fragments arranged by size:
3634 939 552 446 300 243 160 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5616
Fragments arranged by size:
5616 683 56
BtsI GCAGTG_nn'
Cuts at: 3177 3871 4665 4685 3177
Size: 694 794 20 4847
Fragments arranged by size:
4847 794 694 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6355
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6320
DraI TTT'AAA
Cuts at: 3659 3659
Size: 6355
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2354 2369 5362 809
Size: 507 1038 15 2993 1802
Fragments arranged by size:
2993 1802 1038 507 15
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2307 2391 2544
Size: 54 1425 50 275 18 477 84 153
Cuts at: 2544 2610 2625 2901 2914 3202 3237 3802 4691
Size: 66 15 276 13 288 35 565 889
Cuts at: 4691 6320 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 565 477 288 276 275
153 84 66 54 50 43 35 18
15 13
EagI C'GGCC_G
Cuts at: 1537 1812 3802 1537
Size: 275 1990 4090
Fragments arranged by size:
4090 1990 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2054 3149 4285 1353
Size: 701 1095 1136 3423
Fragments arranged by size:
3423 1136 1095 701
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2258 2830 4942 5316 5462 387
Size: 1064 138 669 572 2112 374 146 1280
Fragments arranged by size:
2112 1280 1064 669 572 374 146 138
EcoICRI GAG'CTC
Cuts at: 2890 2890
Size: 6355
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2338 2701 2762 2788 2867 3055
Size: 393 503 203 363 61 26 79 188
Cuts at: 3055 3119 3605 4418 5015 5802 6070 1239
Size: 64 486 813 597 787 268 1524
Fragments arranged by size:
1524 813 787 597 503 486 393 363
268 203 188 79 64 61 26
EcoNI CCTnn'n_nnAGG
Cuts at: 3134 3134
Size: 6355
EcoO109I rG'GnC_Cy
Cuts at: 2206 2234 2246 2247 2401 2485 3139 3440 3580
Size: 28 12 1 154 84 654 301 140
Cuts at: 3580 2206
Size: 4981
Fragments arranged by size:
4981 654 301 154 140 84 28 12
1
EcoRI G'AATT_C
Cuts at: 3664 4042 3664
Size: 378 5977
EcoRV GAT'ATC
Cuts at: 2820 2820
Size: 6355
FseI GG_CCGG'CC
Cuts at: 3243 3243
Size: 6355
FspI TGC'GCA
Cuts at: 4861 4861
Size: 6355
HaeII r_GCGC'y
Cuts at: 1678 2738 5502 1678
Size: 1060 2764 2531
Fragments arranged by size:
2764 2531 1060
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2159 2191 2981 3013 3546
Size: 32 135 32 587 32 790 32 533
Cuts at: 3546 3578 4996 5028 5070 5102 1373
Size: 32 1418 32 42 32 2626
Fragments arranged by size:
2626 1418 790 587 533 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3699 3945 669
Size: 988 281 1761 246 3079
Fragments arranged by size:
3079 1761 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6355
HpaI GTT'AAC
Cuts at: 3945 3945
Size: 6355
KpnI G_GTAC'C
Cuts at: 3680 3680
Size: 6355
MfeI C'AATT_G
Cuts at: 3954 3954
Size: 6355
MluI A'CGCG_T
Cuts at: 6231 6231
Size: 6355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3398 5469 5653 1305
Size: 2093 2071 184 2007
Fragments arranged by size:
2093 2071 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2393 2903 2916 10
Size: 54 1425 343 561 510 13 3449
Fragments arranged by size:
3449 1425 561 510 343 54 13
MslI CAynn'nnrTG
Cuts at: 518 1166 2192 2387 2450 2699 2945 3053 3266
Size: 648 1026 195 63 249 246 108 213
Cuts at: 3266 4313 4672 4831 518
Size: 1047 359 159 2042
Fragments arranged by size:
2042 1047 1026 648 359 249 246 213
195 159 108 63
MspA1I CmG'CkG
Cuts at: 1606 2316 2782 2995 3033 3771 4448 5596 5841
Size: 710 466 213 38 738 677 1148 245
Cuts at: 5841 1606
Size: 2120
Fragments arranged by size:
2120 1148 738 710 677 466 245 213
38
NaeI GCC'GGC
Cuts at: 2629 3241 3422 2629
Size: 612 181 5562
Fragments arranged by size:
5562 612 181
NcoI C'CATG_G
Cuts at: 513 1065 2004 3234 513
Size: 552 939 1230 3634
Fragments arranged by size:
3634 1230 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6355
NgoMIV G'CCGG_C
Cuts at: 2627 3239 3420 2627
Size: 612 181 5562
Fragments arranged by size:
5562 612 181
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6355
NotI GC'GGCC_GC
Cuts at: 3802 3802
Size: 6355
NspI r_CATG'y
Cuts at: 1132 1229 2164 3265 3713 5258 1132
Size: 97 935 1101 448 1545 2229
Fragments arranged by size:
2229 1545 1101 935 448 97
PasI CC'CwG_GG
Cuts at: 1348 2419 3550 3610 1348
Size: 1071 1131 60 4093
Fragments arranged by size:
4093 1131 1071 60
PciI A'CATG_T
Cuts at: 5254 5254
Size: 6355
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2316 3235 3583 1108
Size: 1208 919 348 3880
Fragments arranged by size:
3880 1208 919 348
PfoI T'CCnGG_A
Cuts at: 2112 2456 3540 3576 2112
Size: 344 1084 36 4891
Fragments arranged by size:
4891 1084 344 36
PmeI GTTT'AAAC
Cuts at: 3659 3659
Size: 6355
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4424 4456 5937 5969 4424
Size: 32 1481 32 4810
Fragments arranged by size:
4810 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2401 3440 3580 2401
Size: 1039 140 5176
Fragments arranged by size:
5176 1039 140
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6355
PsiI TTA'TAA
Cuts at: 3925 3925
Size: 6355
PspOMI G'GGCC_C
Cuts at: 2246 2246
Size: 6355
PstI C_TGCA'G
Cuts at: 830 2064 2541 3108 3707 830
Size: 1234 477 567 599 3478
Fragments arranged by size:
3478 1234 599 567 477
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6355
PvuII CAG'CTG
Cuts at: 2316 2782 2316
Size: 466 5889
SacI G_AGCT'C
Cuts at: 2892 2892
Size: 6355
SacII CC_GC'GG
Cuts at: 2996 2996
Size: 6355
SalI G'TCGA_C
Cuts at: 1936 3697 1936
Size: 1761 4594
SanDI GG'GwC_CC
Cuts at: 3440 3440
Size: 6355
SbfI CC_TGCA'GG
Cuts at: 2064 3707 2064
Size: 1643 4712
ScaI AGT'ACT
Cuts at: 4603 6315 4603
Size: 1712 4643
SexAI A'CCwGG_T
Cuts at: 2403 2403
Size: 6355
SfcI C'TryA_G
Cuts at: 826 1045 2060 2537 3104 3703 4838 5519 5710
Size: 219 1015 477 567 599 1135 681 191
Cuts at: 5710 826
Size: 1471
Fragments arranged by size:
1471 1135 1015 681 599 567 477 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6355
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6355
SmaI CCC'GGG
Cuts at: 1872 3682 1872
Size: 1810 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4459 5360 5622 820
Size: 197 926 2516 901 262 1553
Fragments arranged by size:
2516 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4146 493
Size: 3653 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6355
SphI G_CATG'C
Cuts at: 1132 3713 1132
Size: 2581 3774
SspI AAT'ATT
Cuts at: 5 52 4279 5
Size: 47 4227 2081
Fragments arranged by size:
4227 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6355
StyI C'CwwG_G
Cuts at: 513 1065 2004 2202 3199 3234 3794 513
Size: 552 939 198 997 35 560 3074
Fragments arranged by size:
3074 997 939 560 552 198 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4382 4399 4552 4737 5182 325
Size: 1255 2802 17 153 185 445 1498
Fragments arranged by size:
2802 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4601 6313 96
Size: 274 80 33 51 1443 2624 1712 138
Fragments arranged by size:
2624 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2393 2425 6315 6347 2393
Size: 32 3890 32 2401
Fragments arranged by size:
3890 2401 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6355
XbaI T'CTAG_A
Cuts at: 3691 3691
Size: 6355
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2401 2701 2401
Size: 300 6055
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6355
XmaI C'CCGG_G
Cuts at: 1870 3680 1870
Size: 1810 4545
XmnI GAAnn'nnTTC
Cuts at: 3369 4484 3369
Size: 1115 5240
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4163 276
Size: 53 83 186 936 2629 2468
Fragments arranged by size:
2629 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SanDI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII FalI FspAI KasI
NarI NruI NsiI PacI PmlI PspXI PsrI RsrII
SapI SfiI SfoI SrfI SwaI