Restriction map for
pFN21AE4516
(Circular) (Six-base) MAPSORT of: pFN21AE4516.seq Check: 4112 from: 1 to: 6355
pFN21AE4516 6355 bp
With 182 enzymes: SgfI *
March 28, 2012 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2020 2760 2020
Size: 740 5615
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4165 278
Size: 53 83 186 936 2629 2468
Fragments arranged by size:
2629 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2691 3335 3698 1937
Size: 754 644 363 4594
Fragments arranged by size:
4594 754 644 363
Acc65I G'GTAC_C
Cuts at: 3676 3676
Size: 6355
AclI AA'CG_TT
Cuts at: 1563 4482 4855 1563
Size: 2919 373 3063
Fragments arranged by size:
3063 2919 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4418 5802 6070 1632
Size: 2786 1384 268 1917
Fragments arranged by size:
2786 1917 1384 268
AfeI AGC'GCT
Cuts at: 3499 3499
Size: 6355
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6158
AflIII A'CryG_T
Cuts at: 1203 5254 6231 1203
Size: 4051 977 1327
Fragments arranged by size:
4051 1327 977
AgeI A'CCGG_T
Cuts at: 5186 5186
Size: 6355
AhdI GACnn_n'nnGTC
Cuts at: 5084 5084
Size: 6355
AleI CACnn'nnGTG
Cuts at: 1166 2072 1166
Size: 906 5449
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3241 1184
Size: 2057 4298
AlwNI CAG_nnn'CTG
Cuts at: 2761 3538 3559 4098 5670 2761
Size: 777 21 539 1572 3446
Fragments arranged by size:
3446 1572 777 539 21
ApaI G_GGCC'C
Cuts at: 2401 2866 2401
Size: 465 5890
ApaLI G'TGCA_C
Cuts at: 4412 5568 4412
Size: 1156 5199
ApoI r'AATT_y
Cuts at: 1493 3664 3890 4042 6151 1493
Size: 2171 226 152 2109 1697
Fragments arranged by size:
2171 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4909 160
Size: 872 3877 1606
Fragments arranged by size:
3877 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6355
AvaI C'yCGr_G
Cuts at: 1870 1943 2208 2587 3399 3680 1870
Size: 73 265 379 812 281 4545
Fragments arranged by size:
4545 812 379 281 265 73
BamHI G'GATC_C
Cuts at: 3685 4052 3685
Size: 367 5988
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2672 2745 3676 5131 618
Size: 325 217 627 885 73 931 1455 1842
Fragments arranged by size:
1842 1455 931 885 627 325 217 73
BanII G_rGCy'C
Cuts at: 1391 2316 2401 2866 3422 3447 3674 1391
Size: 925 85 465 556 25 227 4072
Fragments arranged by size:
4072 925 556 465 227 85 25
BbeI G_GCGC'C
Cuts at: 2749 2749
Size: 6355
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5384
BbvCI CC'TCA_GC
Cuts at: 2174 3631 2174
Size: 1457 4898
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4546 4580 1903
Size: 34 2609 34 3678
Fragments arranged by size:
3678 2609 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3360 4247 5457 3360
Size: 887 1210 4258
Fragments arranged by size:
4258 1210 887
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6355
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2743 3200 4966
Size: 107 122 71 631 605 1071 457 1766
Cuts at: 4966 136
Size: 1525
Fragments arranged by size:
1766 1525 1071 631 605 457 122 107
71
BglII A'GATC_T
Cuts at: 6350 6350
Size: 6355
BlpI GC'TnA_GC
Cuts at: 3772 3772
Size: 6355
Bme1580I G_kGCm'C
Cuts at: 1790 2401 2675 2739 2866 4416 5572 1790
Size: 611 274 64 127 1550 1156 2573
Fragments arranged by size:
2573 1550 1156 611 274 127 64
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6355
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2410 3348 4086
Size: 416 53 83 399 24 993 938 738
Cuts at: 4086 5044 442
Size: 958 1753
Fragments arranged by size:
1753 993 958 938 738 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6355
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 3581 5015 1239
Size: 974 1368 1434 2579
Fragments arranged by size:
2579 1434 1368 974
Bpu10I CC'TnA_GC
Cuts at: 2174 3631 2174
Size: 1457 4898
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4480 5345 5643 4480
Size: 865 298 5192
Fragments arranged by size:
5192 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2770 3515 5018 882
Size: 617 1271 745 1503 2219
Fragments arranged by size:
2219 1503 1271 745 617
BsaAI yAC'GTr
Cuts at: 493 1204 4146 493
Size: 711 2942 2702
Fragments arranged by size:
2942 2702 711
BsaBI GATnn'nnATC
Cuts at: 6349 6349
Size: 6355
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2746 4162 4544 5179
Size: 53 83 186 936 1213 1416 382 635
Cuts at: 5179 275
Size: 1451
Fragments arranged by size:
1451 1416 1213 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4788 5186 5460 5607 6018 4788
Size: 398 274 147 411 5125
Fragments arranged by size:
5125 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2439 2469 2819 2849 1178
Size: 30 165 30 1036 30 350 30 4684
Fragments arranged by size:
4684 1036 350 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2573 2821 2824 1186
Size: 1387 248 3 4717
Fragments arranged by size:
4717 1387 248 3
BseYI C'CCAG_C
Cuts at: 3289 5558 6054 3289
Size: 2269 496 3590
Fragments arranged by size:
3590 2269 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2302 3189 3572 1163
Size: 573 566 887 383 3946
Fragments arranged by size:
3946 887 573 566 383
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2047 3805 4566 5594 1540
Size: 275 186 46 1758 761 1028 2301
Fragments arranged by size:
2301 1758 1028 761 275 186 46
BsiHKAI G_wGCw'C
Cuts at: 2390 3447 3603 4416 4501 5572 2390
Size: 1057 156 813 85 1071 3173
Fragments arranged by size:
3173 1071 1057 813 156 85
BsmI GAATG_Cn'
Cuts at: 3866 3959 3866
Size: 93 6262
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2316 2390 2401 2675 2739 2866 3422
Size: 399 526 74 11 274 64 127 556
Cuts at: 3422 3447 3603 3674 4416 4501 5572 1391
Size: 25 156 71 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 556 526 399 274 156
127 85 74 71 64 25 11
BspEI T'CCGG_A
Cuts at: 6018 6018
Size: 6355
BspHI T'CATG_A
Cuts at: 4244 5974 4244
Size: 1730 4625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2020 2520 2543 2760 3090 3147 3710 844
Size: 1176 500 23 217 330 57 563 3489
Fragments arranged by size:
3489 1176 563 500 330 217 57 23
BsrBI CCG'CTC
Cuts at: 1394 1823 4242 1394
Size: 429 2419 3507
Fragments arranged by size:
3507 2419 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4850 5024 65
Size: 1183 3602 174 1396
Fragments arranged by size:
3602 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3186 4999 5186 1700
Size: 110 1376 1813 187 2869
Fragments arranged by size:
2869 1813 1376 187 110
BsrGI T'GTAC_A
Cuts at: 96 2424 96
Size: 2328 4027
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6355
BssSI C'ACGA_G
Cuts at: 4415 5427 4415
Size: 1012 5343
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6355
BstBI TT'CG_AA
Cuts at: 5159 5159
Size: 6355
BstEII G'GTnAC_C
Cuts at: 1184 2065 1184
Size: 881 5474
BstXI CCAn_nnnn'nTGG
Cuts at: 3154 3154
Size: 6355
BstYI r'GATC_y
Cuts at: 1972 2058 2331 3685 4052 4437 4454 5906 5992
Size: 86 273 1354 367 385 17 1452 86
Cuts at: 5992 6004 6350 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1354 385 367 346 273 86
86 17 12
BstZ17I GTA'TAC
Cuts at: 3336 3336
Size: 6355
Bsu36I CC'TnA_GG
Cuts at: 2858 2858
Size: 6355
BtgI C'CryG_G
Cuts at: 513 1065 2004 2451 2958 2982 3393 513
Size: 552 939 447 507 24 411 3475
Fragments arranged by size:
3475 939 552 507 447 411 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5616
Fragments arranged by size:
5616 683 56
BtsI GCAGTG_nn'
Cuts at: 2124 2146 2264 3206 3536 3620 3871 4665 4685
Size: 22 118 942 330 84 251 794 20
Cuts at: 4685 2124
Size: 3794
Fragments arranged by size:
3794 942 794 330 251 118 84 22
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6355
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3037 3072 529
Size: 35 2473 35 3812
Fragments arranged by size:
3812 2473 35 35
DraI TTT'AAA
Cuts at: 3659 3659
Size: 6355
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2524 5362 809
Size: 507 1208 2838 1802
Fragments arranged by size:
2838 1802 1208 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2044 2979 3489
Size: 54 1425 50 275 18 214 935 510
Cuts at: 3489 3802 4691 6320 8
Size: 313 889 1629 43
Fragments arranged by size:
1629 1425 935 889 510 313 275 214
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2044 3802 1537
Size: 275 232 1758 4090
Fragments arranged by size:
4090 1758 275 232
EarI CTCTTCn'nnn_
Cuts at: 1353 3023 3470 4285 1353
Size: 1670 447 815 3423
Fragments arranged by size:
3423 1670 815 447
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2999 4942 5316 5462 387
Size: 1064 138 1410 1943 374 146 1280
Fragments arranged by size:
1943 1410 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3445 3445
Size: 6355
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 3581 4418 5015 5802 6070 1239
Size: 393 581 1368 837 597 787 268 1524
Fragments arranged by size:
1524 1368 837 787 597 581 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2027 2839 3113 2027
Size: 812 274 5269
Fragments arranged by size:
5269 812 274
EcoO109I rG'GnC_Cy
Cuts at: 2377 2397 2398 2532 2862 2863 3556 2377
Size: 20 1 134 330 1 693 5176
Fragments arranged by size:
5176 693 330 134 20 1 1
EcoRI G'AATT_C
Cuts at: 3664 4042 3664
Size: 378 5977
FspI TGC'GCA
Cuts at: 2539 4861 2539
Size: 2322 4033
HaeII r_GCGC'y
Cuts at: 1678 2749 3501 5502 1678
Size: 1071 752 2001 2531
Fragments arranged by size:
2531 2001 1071 752
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2086 2118 3087 3119 4996
Size: 32 135 32 514 32 969 32 1877
Cuts at: 4996 5028 5070 5102 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1877 969 514 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3699 3945 669
Size: 988 281 1761 246 3079
Fragments arranged by size:
3079 1761 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3149 748
Size: 2401 3954
HpaI GTT'AAC
Cuts at: 3945 3945
Size: 6355
KasI G'GCGC_C
Cuts at: 2745 2745
Size: 6355
KpnI G_GTAC'C
Cuts at: 3680 3680
Size: 6355
MfeI C'AATT_G
Cuts at: 3954 3954
Size: 6355
MluI A'CGCG_T
Cuts at: 6231 6231
Size: 6355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5469 5653 1305
Size: 4164 184 2007
Fragments arranged by size:
4164 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4533
Fragments arranged by size:
4533 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2072 3380 4313 4672 4831 518
Size: 648 906 1308 933 359 159 2042
Fragments arranged by size:
2042 1308 933 906 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2410 2522 2823 3771 4448 5596 5841 1606
Size: 804 112 301 948 677 1148 245 2120
Fragments arranged by size:
2120 1148 948 804 677 301 245 112
NaeI GCC'GGC
Cuts at: 3188 3188
Size: 6355
NarI GG'CG_CC
Cuts at: 2746 2746
Size: 6355
NcoI C'CATG_G
Cuts at: 513 1065 2004 2451 2958 2982 3393 513
Size: 552 939 447 507 24 411 3475
Fragments arranged by size:
3475 939 552 507 447 411 24
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6355
NgoMIV G'CCGG_C
Cuts at: 3186 3186
Size: 6355
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6355
NotI GC'GGCC_GC
Cuts at: 3802 3802
Size: 6355
NspI r_CATG'y
Cuts at: 1132 1229 3713 5258 1132
Size: 97 2484 1545 2229
Fragments arranged by size:
2484 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3178 3526 1348
Size: 1830 348 4177
Fragments arranged by size:
4177 1830 348
PciI A'CATG_T
Cuts at: 5254 5254
Size: 6355
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3039 1108
Size: 1931 4424
PfoI T'CCnGG_A
Cuts at: 2837 2837
Size: 6355
PmeI GTTT'AAAC
Cuts at: 3659 3659
Size: 6355
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4424 4456 5937 5969 4424
Size: 32 1481 32 4810
Fragments arranged by size:
4810 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2377 2532 3556 2377
Size: 155 1024 5176
Fragments arranged by size:
5176 1024 155
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6355
PsiI TTA'TAA
Cuts at: 3925 3925
Size: 6355
PspOMI G'GGCC_C
Cuts at: 2397 2862 2397
Size: 465 5890
PstI C_TGCA'G
Cuts at: 830 2028 2118 2517 2757 2871 2946 3087 3627
Size: 1198 90 399 240 114 75 141 540
Cuts at: 3627 3707 830
Size: 80 3478
Fragments arranged by size:
3478 1198 540 399 240 141 114 90
80 75
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6355
PvuII CAG'CTG
Cuts at: 2410 2522 2410
Size: 112 6243
SacI G_AGCT'C
Cuts at: 3447 3447
Size: 6355
SalI G'TCGA_C
Cuts at: 1936 3697 1936
Size: 1761 4594
SbfI CC_TGCA'GG
Cuts at: 3087 3707 3087
Size: 620 5735
ScaI AGT'ACT
Cuts at: 4603 6315 4603
Size: 1712 4643
SfcI C'TryA_G
Cuts at: 826 1045 2024 2114 2513 2692 2753 2867 2942
Size: 219 979 90 399 179 61 114 75
Cuts at: 2942 3083 3623 3703 4838 5519 5710 826
Size: 141 540 80 1135 681 191 1471
Fragments arranged by size:
1471 1135 979 681 540 399 219 191
179 141 114 90 80 75 61
SfoI GGC'GCC
Cuts at: 2747 2747
Size: 6355
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6355
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6355
SmaI CCC'GGG
Cuts at: 1872 2210 3682 1872
Size: 338 1472 4545
Fragments arranged by size:
4545 1472 338
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4459 5360 5622 820
Size: 197 926 2516 901 262 1553
Fragments arranged by size:
2516 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4146 493
Size: 3653 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6355
SphI G_CATG'C
Cuts at: 1132 3713 1132
Size: 2581 3774
SspI AAT'ATT
Cuts at: 5 52 4279 5
Size: 47 4227 2081
Fragments arranged by size:
4227 2081 47
StuI AGG'CCT
Cuts at: 1517 3629 1517
Size: 2112 4243
StyI C'CwwG_G
Cuts at: 513 1065 2004 2451 2958 2982 3393 3794 513
Size: 552 939 447 507 24 411 401 3074
Fragments arranged by size:
3074 939 552 507 447 411 401 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2566 4382 4399 4552 4737 5182 325
Size: 1255 986 1816 17 153 185 445 1498
Fragments arranged by size:
1816 1498 1255 986 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2424 4601 6313
Size: 274 80 33 51 1443 447 2177 1712
Cuts at: 6313 96
Size: 138
Fragments arranged by size:
2177 1712 1443 447 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2369 2401 2439 2471 2541 2573 2819 2851 6315
Size: 32 38 32 70 32 246 32 3464
Cuts at: 6315 6347 2369
Size: 32 2377
Fragments arranged by size:
3464 2377 246 70 38 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6355
XbaI T'CTAG_A
Cuts at: 3691 3691
Size: 6355
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2917 3122 3400 2917
Size: 205 278 5872
Fragments arranged by size:
5872 278 205
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6355
XmaI C'CCGG_G
Cuts at: 1870 2208 3680 1870
Size: 338 1472 4545
Fragments arranged by size:
4545 1472 338
XmnI GAAnn'nnTTC
Cuts at: 4484 4484
Size: 6355
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4163 276
Size: 53 83 186 936 2629 2468
Fragments arranged by size:
2629 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
DraIII EcoRV FalI FseI FspAI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SrfI SwaI