Restriction map for
pFN21AE4512
(Circular) (Six-base) MAPSORT of: pFN21AE4512.seq Check: 1316 from: 1 to: 6325
pFN21AE4512 6325 bp
With 182 enzymes: SgfI *
March 28, 2012 14:06 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4135 278
Size: 53 83 186 936 2599 2468
Fragments arranged by size:
2599 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3668 1937
Size: 1731 4594
Acc65I G'GTAC_C
Cuts at: 3646 3646
Size: 6325
AclI AA'CG_TT
Cuts at: 1563 4452 4825 1563
Size: 2889 373 3063
Fragments arranged by size:
3063 2889 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4388 5772 6040 1632
Size: 2756 1384 268 1917
Fragments arranged by size:
2756 1917 1384 268
AfeI AGC'GCT
Cuts at: 3044 3044
Size: 6325
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6128
AflIII A'CryG_T
Cuts at: 1203 5224 6201 1203
Size: 4021 977 1327
Fragments arranged by size:
4021 1327 977
AgeI A'CCGG_T
Cuts at: 2466 5156 2466
Size: 2690 3635
AhdI GACnn_n'nnGTC
Cuts at: 5054 5054
Size: 6325
AleI CACnn'nnGTG
Cuts at: 1166 3279 1166
Size: 2113 4212
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6325
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2786 2818 3020 3052 2786
Size: 32 202 32 6059
Fragments arranged by size:
6059 202 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2429 2987 4068 5640 2429
Size: 558 1081 1572 3114
Fragments arranged by size:
3114 1572 1081 558
ApaLI G'TGCA_C
Cuts at: 4382 5538 4382
Size: 1156 5169
ApoI r'AATT_y
Cuts at: 1493 3634 3860 4012 6121 1493
Size: 2141 226 152 2109 1697
Fragments arranged by size:
2141 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4879 160
Size: 872 3847 1606
Fragments arranged by size:
3847 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6325
AvaI C'yCGr_G
Cuts at: 1870 1943 2881 3177 3650 1870
Size: 73 938 296 473 4545
Fragments arranged by size:
4545 938 473 296 73
BamHI G'GATC_C
Cuts at: 3655 4022 3655
Size: 367 5958
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2911 3646 5101 618
Size: 325 217 627 1124 735 1455 1842
Fragments arranged by size:
1842 1455 1124 735 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3205 3578 3615 3644 1391
Size: 1814 373 37 29 4072
Fragments arranged by size:
4072 1814 373 37 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3140 928
Size: 971 1241 4113
Fragments arranged by size:
4113 1241 971
BbvCI CC'TCA_GC
Cuts at: 2102 2102
Size: 6325
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4516 4550 1903
Size: 34 2579 34 3678
Fragments arranged by size:
3678 2579 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3088 4217 5427 3088
Size: 1129 1210 3986
Fragments arranged by size:
3986 1210 1129
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6325
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3200 4936 136
Size: 107 122 71 631 605 1528 1736 1525
Fragments arranged by size:
1736 1528 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6320 6320
Size: 6325
BlpI GC'TnA_GC
Cuts at: 3742 3742
Size: 6325
Bme1580I G_kGCm'C
Cuts at: 1790 2916 4386 5542 1790
Size: 1126 1470 1156 2573
Fragments arranged by size:
2573 1470 1156 1126
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6325
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4056 5014 442
Size: 416 53 83 399 24 2639 958 1753
Fragments arranged by size:
2639 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6325
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2374 3592 2374
Size: 1218 5107
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2348 2508 2663 3047 3558 3603 4985 1239
Size: 1109 160 155 384 511 45 1382 2579
Fragments arranged by size:
2579 1382 1109 511 384 160 155 45
Bpu10I CC'TnA_GC
Cuts at: 2102 2102
Size: 6325
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2909 4450 5315 5613 2909
Size: 1541 865 298 3621
Fragments arranged by size:
3621 1541 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2621 3441 4988 882
Size: 617 1122 820 1547 2219
Fragments arranged by size:
2219 1547 1122 820 617
BsaAI yAC'GTr
Cuts at: 493 1204 4116 493
Size: 711 2912 2702
Fragments arranged by size:
2912 2702 711
BsaBI GATnn'nnATC
Cuts at: 6319 6319
Size: 6325
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4132 4514 5149 275
Size: 53 83 186 936 2599 382 635 1451
Fragments arranged by size:
2599 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2466 4758 5156 5430 5577 5988 2466
Size: 2292 398 274 147 411 2803
Fragments arranged by size:
2803 2292 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2171 2201 2786 2816 3020
Size: 30 165 30 768 30 585 30 204
Cuts at: 3020 3050 1178
Size: 30 4453
Fragments arranged by size:
4453 768 585 204 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2179 3404 3407 1186
Size: 993 1225 3 4104
Fragments arranged by size:
4104 1225 993 3
BseYI C'CCAG_C
Cuts at: 2448 2481 2829 2858 3167 5528 6024 2448
Size: 33 348 29 309 2361 496 2749
Fragments arranged by size:
2749 2361 496 348 309 33 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2249 2764 3405 1163
Size: 573 513 515 641 4083
Fragments arranged by size:
4083 641 573 515 513
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3775 4536 5564 1540
Size: 275 186 1774 761 1028 2301
Fragments arranged by size:
2301 1774 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2115 4386 4471 5542 2115
Size: 2271 85 1071 2898
Fragments arranged by size:
2898 2271 1071 85
BsmI GAATG_Cn'
Cuts at: 2397 2446 3836 3929 2397
Size: 49 1390 93 4793
Fragments arranged by size:
4793 1390 93 49
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2115 2916 3205 3578 3615 3644 4386
Size: 399 325 801 289 373 37 29 742
Cuts at: 4386 4471 5542 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 801 742 399 373 325 289
85 37 29
BspEI T'CCGG_A
Cuts at: 5988 5988
Size: 6325
BspHI T'CATG_A
Cuts at: 4214 5944 4214
Size: 1730 4595
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2290 2985 3366 3680 844
Size: 1446 695 381 314 3489
Fragments arranged by size:
3489 1446 695 381 314
BsrBI CCG'CTC
Cuts at: 1394 1823 2961 4212 1394
Size: 429 1138 1251 3507
Fragments arranged by size:
3507 1251 1138 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2730 4820 4994 65
Size: 1183 1482 2090 174 1396
Fragments arranged by size:
2090 1482 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2466 2490 4969 5156 1700
Size: 110 656 24 2479 187 2869
Fragments arranged by size:
2869 2479 656 187 110 24
BsrGI T'GTAC_A
Cuts at: 96 3074 96
Size: 2978 3347
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6325
BssSI C'ACGA_G
Cuts at: 4385 5397 4385
Size: 1012 5313
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6325
BstBI TT'CG_AA
Cuts at: 5129 5129
Size: 6325
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6325
BstXI CCAn_nnnn'nTGG
Cuts at: 3268 3281 3268
Size: 13 6312
BstYI r'GATC_y
Cuts at: 1972 3655 4022 4407 4424 5876 5962 5974 6320
Size: 1683 367 385 17 1452 86 12 346
Cuts at: 6320 1972
Size: 1977
Fragments arranged by size:
1977 1683 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2325 2948 2325
Size: 623 5702
BtgI C'CryG_G
Cuts at: 513 1065 2004 2172 513
Size: 552 939 168 4666
Fragments arranged by size:
4666 939 552 168
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5586
Fragments arranged by size:
5586 683 56
BtsI GCAGTG_nn'
Cuts at: 2452 2668 3841 4635 4655 2452
Size: 216 1173 794 20 4122
Fragments arranged by size:
4122 1173 794 216 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6325
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3134 3169 529
Size: 35 2570 35 3685
Fragments arranged by size:
3685 2570 35 35
DraI TTT'AAA
Cuts at: 3629 3629
Size: 6325
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5332 809
Size: 507 4016 1802
Fragments arranged by size:
4016 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3772 4661 6290
Size: 54 1425 50 275 18 1942 889 1629
Cuts at: 6290 8
Size: 43
Fragments arranged by size:
1942 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3772 1537
Size: 275 1960 4090
Fragments arranged by size:
4090 1960 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4255 1353
Size: 2902 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4912 5286 5432 387
Size: 1064 138 3323 374 146 1280
Fragments arranged by size:
3323 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2348 2508 2663 3047 3558 3603 4388
Size: 393 716 160 155 384 511 45 785
Cuts at: 4388 4985 5772 6040 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 785 716 597 511 393 384
268 160 155 45
EcoO109I rG'GnC_Cy
Cuts at: 2312 2312
Size: 6325
EcoRI G'AATT_C
Cuts at: 3634 4012 3634
Size: 378 5947
FspI TGC'GCA
Cuts at: 4831 4831
Size: 6325
HaeII r_GCGC'y
Cuts at: 1678 2956 3046 5472 1678
Size: 1278 90 2426 2531
Fragments arranged by size:
2531 2426 1278 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2864 2896 3423 3455 4966
Size: 32 135 32 1292 32 527 32 1511
Cuts at: 4966 4998 5040 5072 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1511 1292 527 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3493 3669 3915 669
Size: 988 281 1555 176 246 3079
Fragments arranged by size:
3079 1555 988 281 246 176
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6325
HpaI GTT'AAC
Cuts at: 3915 3915
Size: 6325
KpnI G_GTAC'C
Cuts at: 3650 3650
Size: 6325
MfeI C'AATT_G
Cuts at: 3924 3924
Size: 6325
MluI A'CGCG_T
Cuts at: 6201 6201
Size: 6325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2287 5439 5623 1305
Size: 982 3152 184 2007
Fragments arranged by size:
3152 2007 982 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4503
Fragments arranged by size:
4503 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2373 3279 4283 4642 4801 518
Size: 648 1207 906 1004 359 159 2042
Fragments arranged by size:
2042 1207 1004 906 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2101 2661 3025 3348 3741 4418 5566 5811
Size: 495 560 364 323 393 677 1148 245
Cuts at: 5811 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 560 495 393 364 323
245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2172 513
Size: 552 939 168 4666
Fragments arranged by size:
4666 939 552 168
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6325
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6325
NotI GC'GGCC_GC
Cuts at: 3772 3772
Size: 6325
NspI r_CATG'y
Cuts at: 1132 1229 2567 3683 5228 1132
Size: 97 1338 1116 1545 2229
Fragments arranged by size:
2229 1545 1338 1116 97
PasI CC'CwG_GG
Cuts at: 1348 2510 1348
Size: 1162 5163
PciI A'CATG_T
Cuts at: 5224 5224
Size: 6325
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2494 1108
Size: 1386 4939
PfoI T'CCnGG_A
Cuts at: 2435 2640 2814 2435
Size: 205 174 5946
Fragments arranged by size:
5946 205 174
PmeI GTTT'AAAC
Cuts at: 3629 3629
Size: 6325
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2732 2764 2786 2818 3020 3052 4394 4426 5907
Size: 32 22 32 202 32 1342 32 1481
Cuts at: 5907 5939 2732
Size: 32 3118
Fragments arranged by size:
3118 1481 1342 202 32 32 32 32
32 22
PpuMI rG'GwC_Cy
Cuts at: 2312 2312
Size: 6325
PshAI GACnn'nnGTC
Cuts at: 1613 3246 1613
Size: 1633 4692
PsiI TTA'TAA
Cuts at: 3895 3895
Size: 6325
PstI C_TGCA'G
Cuts at: 830 3677 830
Size: 2847 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6325
PvuII CAG'CTG
Cuts at: 2101 3025 2101
Size: 924 5401
SalI G'TCGA_C
Cuts at: 1936 3667 1936
Size: 1731 4594
SanDI GG'GwC_CC
Cuts at: 2312 2312
Size: 6325
SbfI CC_TGCA'GG
Cuts at: 3677 3677
Size: 6325
ScaI AGT'ACT
Cuts at: 4573 6285 4573
Size: 1712 4613
SfcI C'TryA_G
Cuts at: 826 1045 2085 3673 4808 5489 5680 826
Size: 219 1040 1588 1135 681 191 1471
Fragments arranged by size:
1588 1471 1135 1040 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6325
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6325
SmaI CCC'GGG
Cuts at: 1872 3652 1872
Size: 1780 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2888 4429 5330 5592 820
Size: 197 926 945 1541 901 262 1553
Fragments arranged by size:
1553 1541 945 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4116 493
Size: 3623 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6325
SphI G_CATG'C
Cuts at: 1132 3683 1132
Size: 2551 3774
SspI AAT'ATT
Cuts at: 5 52 4249 5
Size: 47 4197 2081
Fragments arranged by size:
4197 2081 47
StuI AGG'CCT
Cuts at: 1517 3086 3377 1517
Size: 1569 291 4465
Fragments arranged by size:
4465 1569 291
StyI C'CwwG_G
Cuts at: 513 1065 2004 2028 2172 2473 3555 3615 3764
Size: 552 939 24 144 301 1082 60 149
Cuts at: 3764 513
Size: 3074
Fragments arranged by size:
3074 1082 939 552 301 149 144 60
24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2743 4352 4369 4522 4707 5152 325
Size: 1255 1163 1609 17 153 185 445 1498
Fragments arranged by size:
1609 1498 1255 1163 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3074 4571 6283
Size: 274 80 33 51 1443 1097 1497 1712
Cuts at: 6283 96
Size: 138
Fragments arranged by size:
1712 1497 1443 1097 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2171 2203 6285 6317 2171
Size: 32 4082 32 2179
Fragments arranged by size:
4082 2179 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2220 1316
Size: 904 5421
XbaI T'CTAG_A
Cuts at: 3661 3661
Size: 6325
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2026 2495 2026
Size: 469 5856
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6325
XmaI C'CCGG_G
Cuts at: 1870 3650 1870
Size: 1780 4545
XmnI GAAnn'nnTTC
Cuts at: 4454 4454
Size: 6325
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4133 276
Size: 53 83 186 936 2599 2468
Fragments arranged by size:
2599 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI