Restriction map for
pFN21AE4511
(Circular) (Six-base) MAPSORT of: pFN21AE4511.seq Check: 3096 from: 1 to: 6322
pFN21AE4511 6322 bp
With 182 enzymes: SgfI *
March 28, 2012 14:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2959 2959
Size: 6322
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4132 278
Size: 53 83 186 936 2596 2468
Fragments arranged by size:
2596 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2089 3665 1937
Size: 152 1576 4594
Fragments arranged by size:
4594 1576 152
Acc65I G'GTAC_C
Cuts at: 3643 3643
Size: 6322
AclI AA'CG_TT
Cuts at: 1563 4449 4822 1563
Size: 2886 373 3063
Fragments arranged by size:
3063 2886 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3158 4385 5769 6037 1632
Size: 1526 1227 1384 268 1917
Fragments arranged by size:
1917 1526 1384 1227 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6125
AflIII A'CryG_T
Cuts at: 1203 2826 5221 6198 1203
Size: 1623 2395 977 1327
Fragments arranged by size:
2395 1623 1327 977
AgeI A'CCGG_T
Cuts at: 5153 5153
Size: 6322
AhdI GACnn_n'nnGTC
Cuts at: 5051 5051
Size: 6322
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6322
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6322
AlwNI CAG_nnn'CTG
Cuts at: 4065 5637 4065
Size: 1572 4750
ApaI G_GGCC'C
Cuts at: 2713 2839 2713
Size: 126 6196
ApaLI G'TGCA_C
Cuts at: 4379 5535 4379
Size: 1156 5166
ApoI r'AATT_y
Cuts at: 1493 2321 2405 2671 2719 2871 3480 3631 3857
Size: 828 84 266 48 152 609 151 226
Cuts at: 3857 4009 6118 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 828 609 266 226 152 152
151 84 48
AseI AT'TA_AT
Cuts at: 160 1032 3402 4876 160
Size: 872 2370 1474 1606
Fragments arranged by size:
2370 1606 1474 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6322
AvaI C'yCGr_G
Cuts at: 1870 1943 3647 1870
Size: 73 1704 4545
Fragments arranged by size:
4545 1704 73
BamHI G'GATC_C
Cuts at: 2209 3652 4019 2209
Size: 1443 367 4512
Fragments arranged by size:
4512 1443 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3643 5098 618
Size: 325 217 627 1856 1455 1842
Fragments arranged by size:
1856 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2713 2839 3121 3641 1391
Size: 1322 126 282 520 4072
Fragments arranged by size:
4072 1322 520 282 126
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5351
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4513 4547 1903
Size: 34 2576 34 3678
Fragments arranged by size:
3678 2576 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4214 5424 4214
Size: 1210 5112
BclI T'GATC_A
Cuts at: 1548 2296 1548
Size: 748 5574
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2708 4933 136
Size: 107 122 71 631 605 1036 2225 1525
Fragments arranged by size:
2225 1525 1036 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6317 6317
Size: 6322
BlpI GC'TnA_GC
Cuts at: 3739 3739
Size: 6322
Bme1580I G_kGCm'C
Cuts at: 1790 2584 2713 2839 4383 5539 1790
Size: 794 129 126 1544 1156 2573
Fragments arranged by size:
2573 1544 1156 794 129 126
BmgBI CAC'GTC
Cuts at: 1318 2827 1318
Size: 1509 4813
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2520 4053 5011
Size: 416 53 83 399 24 1103 1533 958
Cuts at: 5011 442
Size: 1753
Fragments arranged by size:
1753 1533 1103 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6322
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2913 3228 4982 1239
Size: 1674 315 1754 2579
Fragments arranged by size:
2579 1754 1674 315
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2061 4447 5312 5610 2061
Size: 2386 865 298 2773
Fragments arranged by size:
2773 2386 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4985 882
Size: 617 3486 2219
Fragments arranged by size:
3486 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4113 493
Size: 711 2909 2702
Fragments arranged by size:
2909 2702 711
BsaBI GATnn'nnATC
Cuts at: 2528 6316 2528
Size: 3788 2534
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4129 4511 5146 275
Size: 53 83 186 936 2596 382 635 1451
Fragments arranged by size:
2596 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4755 5153 5427 5574 5985 4755
Size: 398 274 147 411 5092
Fragments arranged by size:
5092 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6097
Fragments arranged by size:
6097 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2733 1186
Size: 1547 4775
BseYI C'CCAG_C
Cuts at: 3539 5525 6021 3539
Size: 1986 496 3840
Fragments arranged by size:
3840 1986 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5749
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3772 4533 5561 1540
Size: 275 186 1771 761 1028 2301
Fragments arranged by size:
2301 1771 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3121 3418 4383 4468 5539 3121
Size: 297 965 85 1071 3904
Fragments arranged by size:
3904 1071 965 297 85
BsmI GAATG_Cn'
Cuts at: 2618 3833 3926 2618
Size: 1215 93 5014
Fragments arranged by size:
5014 1215 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2584 2713 2839 3121 3418 3641 4383
Size: 399 794 129 126 282 297 223 742
Cuts at: 4383 4468 5539 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 794 742 399 297 282 223
129 126 85
BspEI T'CCGG_A
Cuts at: 5985 5985
Size: 6322
BspHI T'CATG_A
Cuts at: 4211 5941 4211
Size: 1730 4592
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2959 3677 844
Size: 2115 718 3489
Fragments arranged by size:
3489 2115 718
BsrBI CCG'CTC
Cuts at: 1394 1823 4209 1394
Size: 429 2386 3507
Fragments arranged by size:
3507 2386 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4817 4991 65
Size: 1183 3569 174 1396
Fragments arranged by size:
3569 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4966 5153 1700
Size: 110 3156 187 2869
Fragments arranged by size:
3156 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2389 96
Size: 2293 4029
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6322
BssSI C'ACGA_G
Cuts at: 4382 5394 4382
Size: 1012 5310
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6322
BstBI TT'CG_AA
Cuts at: 5126 5126
Size: 6322
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6322
BstYI r'GATC_y
Cuts at: 1972 2209 3652 4019 4404 4421 5873 5959 5971
Size: 237 1443 367 385 17 1452 86 12
Cuts at: 5971 6317 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1443 385 367 346 237 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3552 3552
Size: 6322
BtgI C'CryG_G
Cuts at: 513 1065 2004 2013 513
Size: 552 939 9 4822
Fragments arranged by size:
4822 939 552 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5583
Fragments arranged by size:
5583 683 56
BtsI GCAGTG_nn'
Cuts at: 2809 3102 3838 4632 4652 2809
Size: 293 736 794 20 4479
Fragments arranged by size:
4479 794 736 293 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6322
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6287
DraI TTT'AAA
Cuts at: 3339 3626 3339
Size: 287 6035
DraIII CAC_nnn'GTG
Cuts at: 3293 3293
Size: 6322
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5329 809
Size: 507 4013 1802
Fragments arranged by size:
4013 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3769 4658 6287
Size: 54 1425 50 275 18 1939 889 1629
Cuts at: 6287 8
Size: 43
Fragments arranged by size:
1939 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3769 1537
Size: 275 1957 4090
Fragments arranged by size:
4090 1957 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2089 4252 1353
Size: 736 2163 3423
Fragments arranged by size:
3423 2163 736
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4909 5283 5429 387
Size: 1064 138 3320 374 146 1280
Fragments arranged by size:
3320 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3119 3119
Size: 6322
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2913 3158 3228 4385 4982 5769 6037
Size: 393 1281 245 70 1157 597 787 268
Cuts at: 6037 1239
Size: 1524
Fragments arranged by size:
1524 1281 1157 787 597 393 268 245
70
EcoO109I rG'GnC_Cy
Cuts at: 2710 2836 2710
Size: 126 6196
EcoRI G'AATT_C
Cuts at: 3631 4009 3631
Size: 378 5944
FspI TGC'GCA
Cuts at: 4828 4828
Size: 6322
HaeII r_GCGC'y
Cuts at: 1678 5469 1678
Size: 3791 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4963 4995 5037 5069 1373
Size: 32 135 32 3391 32 42 32 2626
Fragments arranged by size:
3391 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3666 3912 669
Size: 988 281 1728 246 3079
Fragments arranged by size:
3079 1728 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6322
HpaI GTT'AAC
Cuts at: 3912 3912
Size: 6322
KpnI G_GTAC'C
Cuts at: 3647 3647
Size: 6322
MfeI C'AATT_G
Cuts at: 3921 3921
Size: 6322
MluI A'CGCG_T
Cuts at: 6198 6198
Size: 6322
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5436 5620 1305
Size: 4131 184 2007
Fragments arranged by size:
4131 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4500
Fragments arranged by size:
4500 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2556 4280 4639 4798 518
Size: 648 1390 1724 359 159 2042
Fragments arranged by size:
2042 1724 1390 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3738 4415 5563 5808 1606
Size: 2132 677 1148 245 2120
Fragments arranged by size:
2132 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2013 513
Size: 552 939 9 4822
Fragments arranged by size:
4822 939 552 9
NdeI CA'TA_TG
Cuts at: 387 3433 387
Size: 3046 3276
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6322
NotI GC'GGCC_GC
Cuts at: 3769 3769
Size: 6322
NspI r_CATG'y
Cuts at: 1132 1229 3680 5225 1132
Size: 97 2451 1545 2229
Fragments arranged by size:
2451 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6322
PciI A'CATG_T
Cuts at: 5221 5221
Size: 6322
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3608 1108
Size: 2500 3822
PmeI GTTT'AAAC
Cuts at: 3626 3626
Size: 6322
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4391 4423 5904 5936 4391
Size: 32 1481 32 4777
Fragments arranged by size:
4777 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6322
PsiI TTA'TAA
Cuts at: 2055 2937 3892 2055
Size: 882 955 4485
Fragments arranged by size:
4485 955 882
PspOMI G'GGCC_C
Cuts at: 2709 2835 2709
Size: 126 6196
PstI C_TGCA'G
Cuts at: 830 3674 830
Size: 2844 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6322
SacI G_AGCT'C
Cuts at: 3121 3121
Size: 6322
SalI G'TCGA_C
Cuts at: 1936 3664 1936
Size: 1728 4594
SbfI CC_TGCA'GG
Cuts at: 3674 3674
Size: 6322
ScaI AGT'ACT
Cuts at: 4570 6282 4570
Size: 1712 4610
SfcI C'TryA_G
Cuts at: 826 1045 2038 2259 3670 4805 5486 5677 826
Size: 219 993 221 1411 1135 681 191 1471
Fragments arranged by size:
1471 1411 1135 993 681 221 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6322
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6322
SmaI CCC'GGG
Cuts at: 1872 3649 1872
Size: 1777 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2076 4426 5327 5589 820
Size: 197 926 133 2350 901 262 1553
Fragments arranged by size:
2350 1553 926 901 262 197 133
SnaBI TAC'GTA
Cuts at: 493 4113 493
Size: 3620 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6322
SphI G_CATG'C
Cuts at: 1132 3680 1132
Size: 2548 3774
SspI AAT'ATT
Cuts at: 5 52 2593 2974 3148 3355 4246 5
Size: 47 2541 381 174 207 891 2081
Fragments arranged by size:
2541 2081 891 381 207 174 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6322
StyI C'CwwG_G
Cuts at: 513 1065 2004 2013 2493 3761 513
Size: 552 939 9 480 1268 3074
Fragments arranged by size:
3074 1268 939 552 480 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4349 4366 4519 4704 5149 325
Size: 1255 2769 17 153 185 445 1498
Fragments arranged by size:
2769 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2389 4568 6280
Size: 274 80 33 51 1443 412 2179 1712
Cuts at: 6280 96
Size: 138
Fragments arranged by size:
2179 1712 1443 412 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6282 6314 6282
Size: 32 6290
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6322
XbaI T'CTAG_A
Cuts at: 3658 3658
Size: 6322
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6322
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6322
XmaI C'CCGG_G
Cuts at: 1870 3647 1870
Size: 1777 4545
XmnI GAAnn'nnTTC
Cuts at: 2559 2643 4451 2559
Size: 84 1808 4430
Fragments arranged by size:
4430 1808 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4130 276
Size: 53 83 186 936 2596 2468
Fragments arranged by size:
2596 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI