Restriction map for
pFN21AE4510
(Circular) (Six-base) MAPSORT of: pFN21AE4510.seq Check: 6576 from: 1 to: 6301
pFN21AE4510 6301 bp
With 182 enzymes: SgfI *
July 6, 2012 17:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2099 2099
Size: 6301
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4111 278
Size: 53 83 186 936 2575 2468
Fragments arranged by size:
2575 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3644 1937
Size: 1707 4594
Acc65I G'GTAC_C
Cuts at: 3622 3622
Size: 6301
AclI AA'CG_TT
Cuts at: 1563 4428 4801 1563
Size: 2865 373 3063
Fragments arranged by size:
3063 2865 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2327 3503 3569 4364 5748 6016 1632
Size: 695 1176 66 795 1384 268 1917
Fragments arranged by size:
1917 1384 1176 795 695 268 66
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6104
AflIII A'CryG_T
Cuts at: 1203 5200 6177 1203
Size: 3997 977 1327
Fragments arranged by size:
3997 1327 977
AgeI A'CCGG_T
Cuts at: 3477 5132 3477
Size: 1655 4646
AhdI GACnn_n'nnGTC
Cuts at: 3157 5030 3157
Size: 1873 4428
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6301
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2802 1184
Size: 1618 4683
AlwNI CAG_nnn'CTG
Cuts at: 2064 4044 5616 2064
Size: 1980 1572 2749
Fragments arranged by size:
2749 1980 1572
ApaI G_GGCC'C
Cuts at: 3431 3431
Size: 6301
ApaLI G'TGCA_C
Cuts at: 4358 5514 4358
Size: 1156 5145
ApoI r'AATT_y
Cuts at: 1493 2129 2584 2918 3610 3836 3988 6097 1493
Size: 636 455 334 692 226 152 2109 1697
Fragments arranged by size:
2109 1697 692 636 455 334 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3231 4855 160
Size: 872 2199 1624 1606
Fragments arranged by size:
2199 1624 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
AvaI C'yCGr_G
Cuts at: 1870 1943 3626 1870
Size: 73 1683 4545
Fragments arranged by size:
4545 1683 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2316 2349 2316
Size: 33 6268
BamHI G'GATC_C
Cuts at: 3631 3998 3631
Size: 367 5934
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3622 5077 618
Size: 325 217 627 1835 1455 1842
Fragments arranged by size:
1842 1835 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2088 2868 3431 3620 1391
Size: 697 780 563 189 4072
Fragments arranged by size:
4072 780 697 563 189
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3219 928
Size: 971 1320 4010
Fragments arranged by size:
4010 1320 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4492 4526 1903
Size: 34 2555 34 3678
Fragments arranged by size:
3678 2555 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2344 2469 4193 5403 2344
Size: 125 1724 1210 3242
Fragments arranged by size:
3242 1724 1210 125
BclI T'GATC_A
Cuts at: 1548 2637 1548
Size: 1089 5212
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4912 136
Size: 107 122 71 631 605 3240 1525
Fragments arranged by size:
3240 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6296 6296
Size: 6301
BlpI GC'TnA_GC
Cuts at: 3718 3718
Size: 6301
Bme1580I G_kGCm'C
Cuts at: 1790 3431 4362 5518 1790
Size: 1641 931 1156 2573
Fragments arranged by size:
2573 1641 1156 931
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6301
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3463 4032 4990
Size: 416 53 83 399 24 2046 569 958
Cuts at: 4990 442
Size: 1753
Fragments arranged by size:
2046 1753 958 569 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2163 1056
Size: 1107 5194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2102 3140 4961 1239
Size: 863 1038 1821 2579
Fragments arranged by size:
2579 1821 1038 863
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2717 3107 4426 5291 5589 2717
Size: 390 1319 865 298 3429
Fragments arranged by size:
3429 1319 865 390 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2921 3269 4964 882
Size: 617 1422 348 1695 2219
Fragments arranged by size:
2219 1695 1422 617 348
BsaAI yAC'GTr
Cuts at: 493 1204 4092 493
Size: 711 2888 2702
Fragments arranged by size:
2888 2702 711
BsaBI GATnn'nnATC
Cuts at: 6295 6295
Size: 6301
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4108 4490 5125 275
Size: 53 83 186 936 2575 382 635 1451
Fragments arranged by size:
2575 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2444 3477 4734 5132 5406 5553 5964 2444
Size: 1033 1257 398 274 147 411 2781
Fragments arranged by size:
2781 1257 1033 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2022 2052 1178
Size: 30 165 30 619 30 5427
Fragments arranged by size:
5427 619 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2162 3069 3307 1186
Size: 976 907 238 4180
Fragments arranged by size:
4180 976 907 238
BseYI C'CCAG_C
Cuts at: 2869 3165 5504 6000 2869
Size: 296 2339 496 3170
Fragments arranged by size:
3170 2339 496 296
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5728
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3482 3751 4512 5540 1540
Size: 275 186 1481 269 761 1028 2301
Fragments arranged by size:
2301 1481 1028 761 275 269 186
BsiHKAI G_wGCw'C
Cuts at: 2868 4362 4447 5518 2868
Size: 1494 85 1071 3651
Fragments arranged by size:
3651 1494 1071 85
BsmI GAATG_Cn'
Cuts at: 2791 2877 3812 3905 2791
Size: 86 935 93 5187
Fragments arranged by size:
5187 935 93 86
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2868 3431 3620 4362 4447 5518
Size: 399 298 780 563 189 742 85 1071
Cuts at: 5518 1391
Size: 2174
Fragments arranged by size:
2174 1071 780 742 563 399 298 189
85
BspEI T'CCGG_A
Cuts at: 2444 5964 2444
Size: 3520 2781
BspHI T'CATG_A
Cuts at: 2634 4190 5920 2634
Size: 1556 1730 3015
Fragments arranged by size:
3015 1730 1556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2099 3656 844
Size: 1255 1557 3489
Fragments arranged by size:
3489 1557 1255
BsrBI CCG'CTC
Cuts at: 1394 1823 4188 1394
Size: 429 2365 3507
Fragments arranged by size:
3507 2365 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2113 2277 4796 4970 65
Size: 1183 865 164 2519 174 1396
Fragments arranged by size:
2519 1396 1183 865 174 164
BsrFI r'CCGG_y
Cuts at: 1700 1810 3477 4945 5132 1700
Size: 110 1667 1468 187 2869
Fragments arranged by size:
2869 1667 1468 187 110
BsrGI T'GTAC_A
Cuts at: 96 2769 96
Size: 2673 3628
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6301
BssSI C'ACGA_G
Cuts at: 3501 4361 5373 3501
Size: 860 1012 4429
Fragments arranged by size:
4429 1012 860
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6301
BstBI TT'CG_AA
Cuts at: 5105 5105
Size: 6301
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6301
BstXI CCAn_nnnn'nTGG
Cuts at: 3128 3438 3128
Size: 310 5991
BstYI r'GATC_y
Cuts at: 1972 2262 3364 3631 3998 4383 4400 5852 5938
Size: 290 1102 267 367 385 17 1452 86
Cuts at: 5938 5950 6296 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1102 385 367 346 290 267
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2837 2837
Size: 6301
BtgI C'CryG_G
Cuts at: 513 1065 2004 3347 3378 513
Size: 552 939 1343 31 3436
Fragments arranged by size:
3436 1343 939 552 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5562
Fragments arranged by size:
5562 683 56
BtsI GCAGTG_nn'
Cuts at: 3111 3443 3817 4611 4631 3111
Size: 332 374 794 20 4781
Fragments arranged by size:
4781 794 374 332 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6301
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2077 2112 529
Size: 35 1513 35 4718
Fragments arranged by size:
4718 1513 35 35
DraI TTT'AAA
Cuts at: 2503 2826 3064 3605 2503
Size: 323 238 541 5199
Fragments arranged by size:
5199 541 323 238
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5308 809
Size: 507 3992 1802
Fragments arranged by size:
3992 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 2371 2934
Size: 54 1425 50 275 18 189 352 563
Cuts at: 2934 3375 3440 3748 4637 6266 8
Size: 441 65 308 889 1629 43
Fragments arranged by size:
1629 1425 889 563 441 352 308 275
189 65 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3748 1537
Size: 275 1936 4090
Fragments arranged by size:
4090 1936 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2067 2096 2194 4231 1353
Size: 714 29 98 2037 3423
Fragments arranged by size:
3423 2037 714 98 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4888 5262 5408 387
Size: 1064 138 3299 374 146 1280
Fragments arranged by size:
3299 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2866 2866
Size: 6301
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2327 3140 3503 3569 4364 4961
Size: 393 470 225 813 363 66 795 597
Cuts at: 4961 5748 6016 1239
Size: 787 268 1524
Fragments arranged by size:
1524 813 795 787 597 470 393 363
268 225 66
EcoNI CCTnn'n_nnAGG
Cuts at: 2056 2056
Size: 6301
EcoO109I rG'GnC_Cy
Cuts at: 2119 2119
Size: 6301
EcoRI G'AATT_C
Cuts at: 2584 2918 3610 3988 2584
Size: 334 692 378 4897
Fragments arranged by size:
4897 692 378 334
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3547 3547
Size: 6301
FspI TGC'GCA
Cuts at: 4807 4807
Size: 6301
HaeII r_GCGC'y
Cuts at: 1678 5448 1678
Size: 3770 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3143 3175 3299 3331 4942
Size: 32 135 32 1571 32 124 32 1611
Cuts at: 4942 4974 5016 5048 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1611 1571 135 124 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3096 3151 3597 3645 3891 669
Size: 988 281 1158 55 446 48 246 3079
Fragments arranged by size:
3079 1158 988 446 281 246 55 48
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6301
HpaI GTT'AAC
Cuts at: 3891 3891
Size: 6301
KpnI G_GTAC'C
Cuts at: 3626 3626
Size: 6301
MfeI C'AATT_G
Cuts at: 3900 3900
Size: 6301
MluI A'CGCG_T
Cuts at: 6177 6177
Size: 6301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2208 5415 5599 1305
Size: 903 3207 184 2007
Fragments arranged by size:
3207 2007 903 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2021 2373 3377 3442 10
Size: 54 1425 343 189 352 1004 65 2869
Fragments arranged by size:
2869 1425 1004 352 343 189 65 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3104 4259 4618 4777 518
Size: 648 1938 1155 359 159 2042
Fragments arranged by size:
2042 1938 1155 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3165 3717 4394 5542 5787 1606
Size: 1559 552 677 1148 245 2120
Fragments arranged by size:
2120 1559 1148 677 552 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3347 3378 513
Size: 552 939 1343 31 3436
Fragments arranged by size:
3436 1343 939 552 31
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6301
NheI G'CTAG_C
Cuts at: 1052 2159 1052
Size: 1107 5194
NotI GC'GGCC_GC
Cuts at: 3748 3748
Size: 6301
NspI r_CATG'y
Cuts at: 1132 1229 2704 3659 5204 1132
Size: 97 1475 955 1545 2229
Fragments arranged by size:
2229 1545 1475 955 97
PasI CC'CwG_GG
Cuts at: 1348 3431 1348
Size: 2083 4218
PciI A'CATG_T
Cuts at: 5200 5200
Size: 6301
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6301
PfoI T'CCnGG_A
Cuts at: 2265 2485 2265
Size: 220 6081
PmeI GTTT'AAAC
Cuts at: 3605 3605
Size: 6301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4370 4402 5883 5915 4370
Size: 32 1481 32 4756
Fragments arranged by size:
4756 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6301
PsiI TTA'TAA
Cuts at: 3871 3871
Size: 6301
PspOMI G'GGCC_C
Cuts at: 3427 3427
Size: 6301
PstI C_TGCA'G
Cuts at: 830 3653 830
Size: 2823 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6301
SacI G_AGCT'C
Cuts at: 2868 2868
Size: 6301
SalI G'TCGA_C
Cuts at: 1936 3643 1936
Size: 1707 4594
SapI GCTCTTCn'nnn_
Cuts at: 2096 2096
Size: 6301
SbfI CC_TGCA'GG
Cuts at: 3653 3653
Size: 6301
ScaI AGT'ACT
Cuts at: 4549 6261 4549
Size: 1712 4589
SexAI A'CCwGG_T
Cuts at: 2539 2539
Size: 6301
SfcI C'TryA_G
Cuts at: 826 1045 3649 4784 5465 5656 826
Size: 219 2604 1135 681 191 1471
Fragments arranged by size:
2604 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6301
SmaI CCC'GGG
Cuts at: 1872 3628 1872
Size: 1756 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2732 3086 4405 5306 5568 820
Size: 197 926 789 354 1319 901 262 1553
Fragments arranged by size:
1553 1319 926 901 789 354 262 197
SnaBI TAC'GTA
Cuts at: 493 4092 493
Size: 3599 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6301
SphI G_CATG'C
Cuts at: 1132 3659 1132
Size: 2527 3774
SspI AAT'ATT
Cuts at: 5 52 4225 5
Size: 47 4173 2081
Fragments arranged by size:
4173 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6301
StyI C'CwwG_G
Cuts at: 513 1065 2004 2693 3127 3347 3378 3740 513
Size: 552 939 689 434 220 31 362 3074
Fragments arranged by size:
3074 939 689 552 434 362 220 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2650 4328 4345 4498 4683 5128 325
Size: 1255 1070 1678 17 153 185 445 1498
Fragments arranged by size:
1678 1498 1255 1070 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2310 2769 3194
Size: 274 80 33 51 1443 333 459 425
Cuts at: 3194 4547 6259 96
Size: 1353 1712 138
Fragments arranged by size:
1712 1443 1353 459 425 333 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2022 2054 2439 2471 6261 6293 2022
Size: 32 385 32 3790 32 2030
Fragments arranged by size:
3790 2030 385 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6301
XbaI T'CTAG_A
Cuts at: 3637 3637
Size: 6301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3376 3376
Size: 6301
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6301
XmaI C'CCGG_G
Cuts at: 1870 3626 1870
Size: 1756 4545
XmnI GAAnn'nnTTC
Cuts at: 2918 4430 2918
Size: 1512 4789
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4109 276
Size: 53 83 186 936 2575 2468
Fragments arranged by size:
2575 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspXI PsrI PvuII RsrII SacII SanDI SfiI
SfoI SrfI SwaI