Restriction map for
pFN21AE4509
(Circular) (Six-base) MAPSORT of: pFN21AE4509.seq Check: 3942 from: 1 to: 6301
pFN21AE4509 6301 bp
With 182 enzymes: SgfI *
March 28, 2012 14:06 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4111 278
Size: 53 83 186 936 2575 2468
Fragments arranged by size:
2575 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3644 1937
Size: 1707 4594
Acc65I G'GTAC_C
Cuts at: 3622 3622
Size: 6301
AclI AA'CG_TT
Cuts at: 1563 4428 4801 1563
Size: 2865 373 3063
Fragments arranged by size:
3063 2865 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2432 4364 5748 6016 1632
Size: 800 1932 1384 268 1917
Fragments arranged by size:
1932 1917 1384 800 268
AfeI AGC'GCT
Cuts at: 2044 2133 2044
Size: 89 6212
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6104
AflIII A'CryG_T
Cuts at: 1203 5200 6177 1203
Size: 3997 977 1327
Fragments arranged by size:
3997 1327 977
AgeI A'CCGG_T
Cuts at: 5132 5132
Size: 6301
AhdI GACnn_n'nnGTC
Cuts at: 5030 5030
Size: 6301
AleI CACnn'nnGTG
Cuts at: 1166 3383 3386 1166
Size: 2217 3 4081
Fragments arranged by size:
4081 2217 3
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2122 3486 1184
Size: 938 1364 3999
Fragments arranged by size:
3999 1364 938
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2922 2954 2922
Size: 32 6269
AlwNI CAG_nnn'CTG
Cuts at: 4044 5616 4044
Size: 1572 4729
ApaI G_GGCC'C
Cuts at: 2070 2070
Size: 6301
ApaLI G'TGCA_C
Cuts at: 4358 5514 4358
Size: 1156 5145
ApoI r'AATT_y
Cuts at: 1493 3610 3836 3988 6097 1493
Size: 2117 226 152 2109 1697
Fragments arranged by size:
2117 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4855 160
Size: 872 3823 1606
Fragments arranged by size:
3823 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
AvaI C'yCGr_G
Cuts at: 1870 1943 2333 2476 3626 1870
Size: 73 390 143 1150 4545
Fragments arranged by size:
4545 1150 390 143 73
BamHI G'GATC_C
Cuts at: 2429 3631 3998 2429
Size: 1202 367 4732
Fragments arranged by size:
4732 1202 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2124 2306 3622 5077 618
Size: 325 217 627 337 182 1316 1455 1842
Fragments arranged by size:
1842 1455 1316 627 337 325 217 182
BanII G_rGCy'C
Cuts at: 1391 2070 2077 2199 3207 3620 1391
Size: 679 7 122 1008 413 4072
Fragments arranged by size:
4072 1008 679 413 122 7
BbeI G_GCGC'C
Cuts at: 2128 2310 2128
Size: 182 6119
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2745 928
Size: 971 846 4484
Fragments arranged by size:
4484 971 846
BbvCI CC'TCA_GC
Cuts at: 3474 3474
Size: 6301
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2338 2372 2764 2798 4492 4526 1903
Size: 34 401 34 392 34 1694 34 3678
Fragments arranged by size:
3678 1694 401 392 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4193 5403 4193
Size: 1210 5091
BclI T'GATC_A
Cuts at: 1548 2268 1548
Size: 720 5581
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4912 136
Size: 107 122 71 631 605 3240 1525
Fragments arranged by size:
3240 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6296 6296
Size: 6301
BlpI GC'TnA_GC
Cuts at: 2198 3040 3718 2198
Size: 842 678 4781
Fragments arranged by size:
4781 842 678
Bme1580I G_kGCm'C
Cuts at: 1790 2070 3571 4362 5518 1790
Size: 280 1501 791 1156 2573
Fragments arranged by size:
2573 1501 1156 791 280
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6301
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4032 4990 442
Size: 416 53 83 399 24 2615 958 1753
Fragments arranged by size:
2615 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6301
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2759 3305 2759
Size: 546 5755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2489 3019 3300 3329 4961 1239
Size: 1250 530 281 29 1632 2579
Fragments arranged by size:
2579 1632 1250 530 281 29
Bpu10I CC'TnA_GC
Cuts at: 2046 2204 3474 2046
Size: 158 1270 4873
Fragments arranged by size:
4873 1270 158
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4426 5291 5589 4426
Size: 865 298 5138
Fragments arranged by size:
5138 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2015 4964 882
Size: 617 516 2949 2219
Fragments arranged by size:
2949 2219 617 516
BsaAI yAC'GTr
Cuts at: 493 1204 2574 2809 4092 493
Size: 711 1370 235 1283 2702
Fragments arranged by size:
2702 1370 1283 711 235
BsaBI GATnn'nnATC
Cuts at: 6295 6295
Size: 6301
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2125 2307 2338 4108
Size: 53 83 186 936 592 182 31 1770
Cuts at: 4108 4490 5125 275
Size: 382 635 1451
Fragments arranged by size:
1770 1451 936 635 592 382 186 182
83 53 31
BsaWI w'CCGG_w
Cuts at: 2432 3372 4734 5132 5406 5553 5964 2432
Size: 940 1362 398 274 147 411 2769
Fragments arranged by size:
2769 1362 940 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2922 2952 1178
Size: 30 165 30 1519 30 4527
Fragments arranged by size:
4527 1519 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2475 2990 3527 3530 1186
Size: 1289 515 537 3 3957
Fragments arranged by size:
3957 1289 537 515 3
BseYI C'CCAG_C
Cuts at: 5504 6000 5504
Size: 496 5805
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3435 1163
Size: 573 1699 4029
Fragments arranged by size:
4029 1699 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2064 3751 4512 5540 1540
Size: 275 186 63 1687 761 1028 2301
Fragments arranged by size:
2301 1687 1028 761 275 186 63
BsiHKAI G_wGCw'C
Cuts at: 2077 3207 4362 4447 5518 2077
Size: 1130 1155 85 1071 2860
Fragments arranged by size:
2860 1155 1130 1071 85
BsiWI C'GTAC_G
Cuts at: 2570 2570
Size: 6301
BsmI GAATG_Cn'
Cuts at: 3420 3812 3905 3420
Size: 392 93 5816
Fragments arranged by size:
5816 392 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2495 2495
Size: 6301
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2077 2199 3207 3571 3620 4362
Size: 399 280 7 122 1008 364 49 742
Cuts at: 4362 4447 5518 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1008 742 399 364 280 122
85 49 7
BspEI T'CCGG_A
Cuts at: 5964 5964
Size: 6301
BspHI T'CATG_A
Cuts at: 3331 4190 5920 3331
Size: 859 1730 3712
Fragments arranged by size:
3712 1730 859
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2206 3038 3096 3345 3656 844
Size: 1362 832 58 249 311 3489
Fragments arranged by size:
3489 1362 832 311 249 58
BsrBI CCG'CTC
Cuts at: 1394 1823 4188 1394
Size: 429 2365 3507
Fragments arranged by size:
3507 2365 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3180 3294 4796 4970 65
Size: 1183 1932 114 1502 174 1396
Fragments arranged by size:
1932 1502 1396 1183 174 114
BsrFI r'CCGG_y
Cuts at: 1700 1810 4945 5132 1700
Size: 110 3135 187 2869
Fragments arranged by size:
3135 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6301
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6301
BssSI C'ACGA_G
Cuts at: 4361 5373 4361
Size: 1012 5289
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6301
BstBI TT'CG_AA
Cuts at: 5105 5105
Size: 6301
BstEII G'GTnAC_C
Cuts at: 1184 2218 2875 1184
Size: 1034 657 4610
Fragments arranged by size:
4610 1034 657
BstXI CCAn_nnnn'nTGG
Cuts at: 2461 2554 3307 3385 2461
Size: 93 753 78 5377
Fragments arranged by size:
5377 753 93 78
BstYI r'GATC_y
Cuts at: 1972 2429 2637 3631 3998 4383 4400 5852 5938
Size: 457 208 994 367 385 17 1452 86
Cuts at: 5938 5950 6296 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 994 457 385 367 346 208
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2085 2400 3171 3381 3558 513
Size: 552 939 81 315 771 210 177 3256
Fragments arranged by size:
3256 939 771 552 315 210 177 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2335 3507 487
Size: 683 56 1109 1172 3281
Fragments arranged by size:
3281 1172 1109 683 56
BtsI GCAGTG_nn'
Cuts at: 3817 4611 4631 3817
Size: 794 20 5487
Fragments arranged by size:
5487 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6301
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6266
DraI TTT'AAA
Cuts at: 3605 3605
Size: 6301
DraIII CAC_nnn'GTG
Cuts at: 2384 2384
Size: 6301
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2449 5308 809
Size: 507 1133 2859 1802
Fragments arranged by size:
2859 1802 1133 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 2526 3168
Size: 54 1425 50 275 18 231 465 642
Cuts at: 3168 3561 3748 4637 6266 8
Size: 393 187 889 1629 43
Fragments arranged by size:
1629 1425 889 642 465 393 275 231
187 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2061 3748 1537
Size: 275 249 1687 4090
Fragments arranged by size:
4090 1687 275 249
EarI CTCTTCn'nnn_
Cuts at: 1353 2006 3069 4231 1353
Size: 653 1063 1162 3423
Fragments arranged by size:
3423 1162 1063 653
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4888 5262 5408 387
Size: 1064 138 3299 374 146 1280
Fragments arranged by size:
3299 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2075 3205 2075
Size: 1130 5171
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2432 2489 3019 3300 3329 4364 4961
Size: 393 800 57 530 281 29 1035 597
Cuts at: 4961 5748 6016 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1035 800 787 597 530 393 281
268 57 29
EcoNI CCTnn'n_nnAGG
Cuts at: 2768 2768
Size: 6301
EcoO109I rG'GnC_Cy
Cuts at: 2054 2480 3286 2054
Size: 426 806 5069
Fragments arranged by size:
5069 806 426
EcoRI G'AATT_C
Cuts at: 3610 3988 3610
Size: 378 5923
FspI TGC'GCA
Cuts at: 2264 2439 3186 4807 2264
Size: 175 747 1621 3758
Fragments arranged by size:
3758 1621 747 175
HaeII r_GCGC'y
Cuts at: 1678 2046 2128 2135 2310 2396 5448 1678
Size: 368 82 7 175 86 3052 2531
Fragments arranged by size:
3052 2531 368 175 86 82 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3138 3170 3506 3538 4942
Size: 32 135 32 1566 32 336 32 1404
Cuts at: 4942 4974 5016 5048 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1566 1404 336 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3645 3891 669
Size: 988 281 1707 246 3079
Fragments arranged by size:
3079 1707 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6301
HpaI GTT'AAC
Cuts at: 3891 3891
Size: 6301
KasI G'GCGC_C
Cuts at: 2124 2306 2124
Size: 182 6119
KpnI G_GTAC'C
Cuts at: 3626 3626
Size: 6301
MfeI C'AATT_G
Cuts at: 3900 3900
Size: 6301
MluI A'CGCG_T
Cuts at: 6177 6177
Size: 6301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2815 3238 5415 5599 1305
Size: 1510 423 2177 184 2007
Fragments arranged by size:
2177 2007 1510 423 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2528 3563 10
Size: 54 1425 343 696 1035 2748
Fragments arranged by size:
2748 1425 1035 696 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2867 3233 3383 3386 4259 4618 4777
Size: 648 1701 366 150 3 873 359 159
Cuts at: 4777 518
Size: 2042
Fragments arranged by size:
2042 1701 873 648 366 359 159 150
3
MspA1I CmG'CkG
Cuts at: 1606 2087 2281 2328 3173 3278 3551 3717 4394
Size: 481 194 47 845 105 273 166 677
Cuts at: 4394 5542 5787 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 845 677 481 273 245 194
166 105 47
NarI GG'CG_CC
Cuts at: 2125 2307 2125
Size: 182 6119
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4810
Fragments arranged by size:
4810 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6301
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6301
NotI GC'GGCC_GC
Cuts at: 3748 3748
Size: 6301
NspI r_CATG'y
Cuts at: 1132 1229 2260 3659 5204 1132
Size: 97 1031 1399 1545 2229
Fragments arranged by size:
2229 1545 1399 1031 97
PasI CC'CwG_GG
Cuts at: 1348 3409 1348
Size: 2061 4240
PciI A'CATG_T
Cuts at: 5200 5200
Size: 6301
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2134 3166 1108
Size: 1026 1032 4243
Fragments arranged by size:
4243 1032 1026
PfoI T'CCnGG_A
Cuts at: 2792 3306 2792
Size: 514 5787
PmeI GTTT'AAAC
Cuts at: 3605 3605
Size: 6301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2597 2629 2922 2954 4370 4402 5883 5915 2597
Size: 32 293 32 1416 32 1481 32 2983
Fragments arranged by size:
2983 1481 1416 293 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3286 3286
Size: 6301
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6301
PsiI TTA'TAA
Cuts at: 2589 3871 2589
Size: 1282 5019
PspOMI G'GGCC_C
Cuts at: 2066 2066
Size: 6301
PstI C_TGCA'G
Cuts at: 830 2294 3653 830
Size: 1464 1359 3478
Fragments arranged by size:
3478 1464 1359
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6301
PvuII CAG'CTG
Cuts at: 2328 3278 2328
Size: 950 5351
SacI G_AGCT'C
Cuts at: 2077 3207 2077
Size: 1130 5171
SacII CC_GC'GG
Cuts at: 2088 3174 2088
Size: 1086 5215
SalI G'TCGA_C
Cuts at: 1936 3643 1936
Size: 1707 4594
SapI GCTCTTCn'nnn_
Cuts at: 2006 2006
Size: 6301
SbfI CC_TGCA'GG
Cuts at: 3653 3653
Size: 6301
ScaI AGT'ACT
Cuts at: 4549 6261 4549
Size: 1712 4589
SfcI C'TryA_G
Cuts at: 826 1045 2290 3649 4784 5465 5656 826
Size: 219 1245 1359 1135 681 191 1471
Fragments arranged by size:
1471 1359 1245 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2126 2308 2126
Size: 182 6119
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6301
SmaI CCC'GGG
Cuts at: 1872 3628 1872
Size: 1756 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4405 5306 5568 820
Size: 197 926 2462 901 262 1553
Fragments arranged by size:
2462 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2574 2809 4092 493
Size: 2081 235 1283 2702
Fragments arranged by size:
2702 2081 1283 235
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6301
SphI G_CATG'C
Cuts at: 1132 2260 3659 1132
Size: 1128 1399 3774
Fragments arranged by size:
3774 1399 1128
SspI AAT'ATT
Cuts at: 5 52 4225 5
Size: 47 4173 2081
Fragments arranged by size:
4173 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6301
StyI C'CwwG_G
Cuts at: 513 1065 2004 2406 3585 3740 513
Size: 552 939 402 1179 155 3074
Fragments arranged by size:
3074 1179 939 552 402 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3401 4328 4345 4498 4683 5128 325
Size: 1255 1821 927 17 153 185 445 1498
Fragments arranged by size:
1821 1498 1255 927 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4547 6259 96
Size: 274 80 33 51 1443 2570 1712 138
Fragments arranged by size:
2570 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6261 6293 6261
Size: 32 6269
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6301
XbaI T'CTAG_A
Cuts at: 3637 3637
Size: 6301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2503 2924 3136 3548 2503
Size: 421 212 412 5256
Fragments arranged by size:
5256 421 412 212
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6301
XmaI C'CCGG_G
Cuts at: 1870 3626 1870
Size: 1756 4545
XmnI GAAnn'nnTTC
Cuts at: 4430 4430
Size: 6301
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4109 276
Size: 53 83 186 936 2575 2468
Fragments arranged by size:
2575 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BfrBI BstZ17I Bsu36I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SanDI SexAI SfiI SrfI
SwaI