Restriction map for
pFN21AE4508
(Circular) (Six-base) MAPSORT of: pFN21AE4508.seq Check: 2973 from: 1 to: 6301
pFN21AE4508 6301 bp
With 182 enzymes: SgfI *
March 28, 2012 14:06 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4111 278
Size: 53 83 186 936 2575 2468
Fragments arranged by size:
2575 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3644 1937
Size: 1707 4594
Acc65I G'GTAC_C
Cuts at: 3622 3622
Size: 6301
AclI AA'CG_TT
Cuts at: 1563 4428 4801 1563
Size: 2865 373 3063
Fragments arranged by size:
3063 2865 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2244 3479 4364 5748 6016 1632
Size: 612 1235 885 1384 268 1917
Fragments arranged by size:
1917 1384 1235 885 612 268
AfeI AGC'GCT
Cuts at: 3136 3136
Size: 6301
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6104
AflIII A'CryG_T
Cuts at: 1203 2565 5200 6177 1203
Size: 1362 2635 977 1327
Fragments arranged by size:
2635 1362 1327 977
AgeI A'CCGG_T
Cuts at: 5132 5132
Size: 6301
AhdI GACnn_n'nnGTC
Cuts at: 5030 5030
Size: 6301
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6301
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6301
AlwNI CAG_nnn'CTG
Cuts at: 2222 2960 2963 2972 4044 5616 2222
Size: 738 3 9 1072 1572 2907
Fragments arranged by size:
2907 1572 1072 738 9 3
ApaLI G'TGCA_C
Cuts at: 4358 5514 4358
Size: 1156 5145
ApoI r'AATT_y
Cuts at: 1493 2141 2779 3340 3610 3836 3988 6097 1493
Size: 648 638 561 270 226 152 2109 1697
Fragments arranged by size:
2109 1697 648 638 561 270 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4855 160
Size: 872 3823 1606
Fragments arranged by size:
3823 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
AvaI C'yCGr_G
Cuts at: 1870 1943 3258 3626 1870
Size: 73 1315 368 4545
Fragments arranged by size:
4545 1315 368 73
BamHI G'GATC_C
Cuts at: 3332 3631 3998 3332
Size: 299 367 5635
Fragments arranged by size:
5635 367 299
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2498 3622 5077 618
Size: 325 217 627 711 1124 1455 1842
Fragments arranged by size:
1842 1455 1124 711 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2923 3620 1391
Size: 1532 697 4072
Fragments arranged by size:
4072 1532 697
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5330
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4492 4526 1903
Size: 34 2555 34 3678
Fragments arranged by size:
3678 2555 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4193 5403 4193
Size: 1210 5091
BclI T'GATC_A
Cuts at: 1548 3274 1548
Size: 1726 4575
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4912 136
Size: 107 122 71 631 605 3240 1525
Fragments arranged by size:
3240 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6296 6296
Size: 6301
BlpI GC'TnA_GC
Cuts at: 3718 3718
Size: 6301
Bme1580I G_kGCm'C
Cuts at: 1790 3446 4362 5518 1790
Size: 1656 916 1156 2573
Fragments arranged by size:
2573 1656 1156 916
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6301
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3447 4032 4990
Size: 416 53 83 399 24 2030 585 958
Cuts at: 4990 442
Size: 1753
Fragments arranged by size:
2030 1753 958 585 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2511 2657 3015 4961 1239
Size: 1272 146 358 1946 2579
Fragments arranged by size:
2579 1946 1272 358 146
Bpu10I CC'TnA_GC
Cuts at: 2442 2442
Size: 6301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4426 5291 5589 4426
Size: 865 298 5138
Fragments arranged by size:
5138 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4964 882
Size: 617 3465 2219
Fragments arranged by size:
3465 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4092 493
Size: 711 2888 2702
Fragments arranged by size:
2888 2702 711
BsaBI GATnn'nnATC
Cuts at: 6295 6295
Size: 6301
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4108 4490 5125 275
Size: 53 83 186 936 2575 382 635 1451
Fragments arranged by size:
2575 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4734 5132 5406 5553 5964 4734
Size: 398 274 147 411 5071
Fragments arranged by size:
5071 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6076
Fragments arranged by size:
6076 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2478 1186
Size: 1292 5009
BseYI C'CCAG_C
Cuts at: 2866 5504 6000 2866
Size: 2638 496 3167
Fragments arranged by size:
3167 2638 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2538 3119 1163
Size: 573 802 581 4345
Fragments arranged by size:
4345 802 581 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3751 4512 5540 1540
Size: 275 186 1750 761 1028 2301
Fragments arranged by size:
2301 1750 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2173 2443 2923 4362 4447 5518 2173
Size: 270 480 1439 85 1071 2956
Fragments arranged by size:
2956 1439 1071 480 270 85
BsmI GAATG_Cn'
Cuts at: 3812 3905 3812
Size: 93 6208
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2173 2443 2923 3446 3620 4362 4447
Size: 399 383 270 480 523 174 742 85
Cuts at: 4447 5518 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 523 480 399 383 270
174 85
BspEI T'CCGG_A
Cuts at: 5964 5964
Size: 6301
BspHI T'CATG_A
Cuts at: 4190 5920 4190
Size: 1730 4571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2863 3656 844
Size: 2019 793 3489
Fragments arranged by size:
3489 2019 793
BsrBI CCG'CTC
Cuts at: 1394 1823 4188 1394
Size: 429 2365 3507
Fragments arranged by size:
3507 2365 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3308 4796 4970 65
Size: 1183 2060 1488 174 1396
Fragments arranged by size:
2060 1488 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4945 5132 1700
Size: 110 3135 187 2869
Fragments arranged by size:
3135 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3247 96
Size: 3151 3150
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6301
BssSI C'ACGA_G
Cuts at: 2178 4361 5373 2178
Size: 2183 1012 3106
Fragments arranged by size:
3106 2183 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2885 2960 2963 2972 794
Size: 2091 75 3 9 4123
Fragments arranged by size:
4123 2091 75 9 3
BstBI TT'CG_AA
Cuts at: 2194 5105 2194
Size: 2911 3390
BstEII G'GTnAC_C
Cuts at: 1184 2257 1184
Size: 1073 5228
BstYI r'GATC_y
Cuts at: 1972 2048 3332 3412 3631 3998 4383 4400 5852
Size: 76 1284 80 219 367 385 17 1452
Cuts at: 5852 5938 5950 6296 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1284 385 367 346 219 86
80 76 17 12
Bsu36I CC'TnA_GG
Cuts at: 2753 2753
Size: 6301
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4810
Fragments arranged by size:
4810 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5562
Fragments arranged by size:
5562 683 56
BtsI GCAGTG_nn'
Cuts at: 2077 2526 3817 4611 4631 2077
Size: 449 1291 794 20 3747
Fragments arranged by size:
3747 1291 794 449 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6301
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6266
DraI TTT'AAA
Cuts at: 2146 3039 3605 2146
Size: 893 566 4842
Fragments arranged by size:
4842 893 566
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2015 5308 809
Size: 507 699 3293 1802
Fragments arranged by size:
3293 1802 699 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2934 2977 3748
Size: 54 1425 50 275 18 1104 43 771
Cuts at: 3748 4637 6266 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1104 889 771 275 54 50
43 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3748 1537
Size: 275 1936 4090
Fragments arranged by size:
4090 1936 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2036 3039 3083 3402 3495 4231 1353
Size: 683 1003 44 319 93 736 3423
Fragments arranged by size:
3423 1003 736 683 319 93 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4888 5262 5408 387
Size: 1064 138 3299 374 146 1280
Fragments arranged by size:
3299 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2921 2921
Size: 6301
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2244 2511 2657 3015 3479 4364 4961
Size: 393 612 267 146 358 464 885 597
Cuts at: 4961 5748 6016 1239
Size: 787 268 1524
Fragments arranged by size:
1524 885 787 612 597 464 393 358
268 267 146
EcoRI G'AATT_C
Cuts at: 3610 3988 3610
Size: 378 5923
FspI TGC'GCA
Cuts at: 4807 4807
Size: 6301
HaeII r_GCGC'y
Cuts at: 1678 3138 5448 1678
Size: 1460 2310 2531
Fragments arranged by size:
2531 2310 1460
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2347 2379 2822 2854 4942
Size: 32 135 32 775 32 443 32 2088
Cuts at: 4942 4974 5016 5048 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2088 775 443 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3594 3645 3891 669
Size: 988 281 1656 51 246 3079
Fragments arranged by size:
3079 1656 988 281 246 51
HindIII A'AGCT_T
Cuts at: 748 2664 748
Size: 1916 4385
HpaI GTT'AAC
Cuts at: 3891 3891
Size: 6301
KpnI G_GTAC'C
Cuts at: 3626 3626
Size: 6301
MfeI C'AATT_G
Cuts at: 3597 3900 3597
Size: 303 5998
MluI A'CGCG_T
Cuts at: 6177 6177
Size: 6301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5415 5599 1305
Size: 4110 184 2007
Fragments arranged by size:
4110 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2936 10
Size: 54 1425 343 1104 3375
Fragments arranged by size:
3375 1425 1104 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2270 3189 4259 4618 4777 518
Size: 648 1104 919 1070 359 159 2042
Fragments arranged by size:
2042 1104 1070 919 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2468 2960 2969 3005 3717 4394 5542 5787
Size: 862 492 9 36 712 677 1148 245
Cuts at: 5787 1606
Size: 2120
Fragments arranged by size:
2120 1148 862 712 677 492 245 36
9
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4810
Fragments arranged by size:
4810 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6301
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6301
NotI GC'GGCC_GC
Cuts at: 3748 3748
Size: 6301
NspI r_CATG'y
Cuts at: 1132 1229 2569 3659 5204 1132
Size: 97 1340 1090 1545 2229
Fragments arranged by size:
2229 1545 1340 1090 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6301
PciI A'CATG_T
Cuts at: 2565 5200 2565
Size: 2635 3666
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6301
PfoI T'CCnGG_A
Cuts at: 2914 2914
Size: 6301
PmeI GTTT'AAAC
Cuts at: 3605 3605
Size: 6301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4370 4402 5883 5915 4370
Size: 32 1481 32 4756
Fragments arranged by size:
4756 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2468 1613
Size: 855 5446
PsiI TTA'TAA
Cuts at: 2736 3549 3871 2736
Size: 813 322 5166
Fragments arranged by size:
5166 813 322
PstI C_TGCA'G
Cuts at: 830 2881 2956 2968 3653 830
Size: 2051 75 12 685 3478
Fragments arranged by size:
3478 2051 685 75 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6301
PvuII CAG'CTG
Cuts at: 2468 2960 2969 3005 2468
Size: 492 9 36 5764
Fragments arranged by size:
5764 492 36 9
SacI G_AGCT'C
Cuts at: 2923 2923
Size: 6301
SalI G'TCGA_C
Cuts at: 1936 3643 1936
Size: 1707 4594
SapI GCTCTTCn'nnn_
Cuts at: 3495 3495
Size: 6301
SbfI CC_TGCA'GG
Cuts at: 3653 3653
Size: 6301
ScaI AGT'ACT
Cuts at: 2393 2544 4549 6261 2393
Size: 151 2005 1712 2433
Fragments arranged by size:
2433 2005 1712 151
SfcI C'TryA_G
Cuts at: 826 1045 2877 2952 2964 3649 4784 5465 5656
Size: 219 1832 75 12 685 1135 681 191
Cuts at: 5656 826
Size: 1471
Fragments arranged by size:
1832 1471 1135 685 681 219 191 75
12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6301
SmaI CCC'GGG
Cuts at: 1872 3628 1872
Size: 1756 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3258 4405 5306 5568 820
Size: 197 926 1315 1147 901 262 1553
Fragments arranged by size:
1553 1315 1147 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4092 493
Size: 3599 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6301
SphI G_CATG'C
Cuts at: 1132 3659 1132
Size: 2527 3774
SspI AAT'ATT
Cuts at: 5 52 2604 2731 4225 5
Size: 47 2552 127 1494 2081
Fragments arranged by size:
2552 2081 1494 127 47
StuI AGG'CCT
Cuts at: 1517 3527 1517
Size: 2010 4291
StyI C'CwwG_G
Cuts at: 513 1065 2004 2889 3553 3740 513
Size: 552 939 885 664 187 3074
Fragments arranged by size:
3074 939 885 664 552 187
SwaI ATTT'AAAT
Cuts at: 3039 3039
Size: 6301
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4328 4345 4498 4683 5128 325
Size: 1255 2748 17 153 185 445 1498
Fragments arranged by size:
2748 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2182 2391 2542
Size: 274 80 33 51 1443 205 209 151
Cuts at: 2542 2568 3247 4547 6259 96
Size: 26 679 1300 1712 138
Fragments arranged by size:
1712 1443 1300 679 274 209 205 151
138 80 51 33 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6261 6293 6261
Size: 32 6269
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6301
XbaI T'CTAG_A
Cuts at: 3637 3637
Size: 6301
XhoI C'TCGA_G
Cuts at: 1943 3258 1943
Size: 1315 4986
XmaI C'CCGG_G
Cuts at: 1870 3626 1870
Size: 1756 4545
XmnI GAAnn'nnTTC
Cuts at: 3519 4430 3519
Size: 911 5390
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4109 276
Size: 53 83 186 936 2575 2468
Fragments arranged by size:
2575 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
XcmI