Restriction map for
pFN21AE4507
(Circular) (Six-base) MAPSORT of: pFN21AE4507.seq Check: 8061 from: 1 to: 6295
pFN21AE4507 6295 bp
With 182 enzymes: SgfI *
March 28, 2012 14:06 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2024 4105 278
Size: 53 83 186 936 488 2081 2468
Fragments arranged by size:
2468 2081 936 488 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3638 1937
Size: 1701 4594
Acc65I G'GTAC_C
Cuts at: 3616 3616
Size: 6295
AclI AA'CG_TT
Cuts at: 1563 4422 4795 1563
Size: 2859 373 3063
Fragments arranged by size:
3063 2859 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2714 4358 5742 6010 1632
Size: 1082 1644 1384 268 1917
Fragments arranged by size:
1917 1644 1384 1082 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6098
AflIII A'CryG_T
Cuts at: 1203 2952 5194 6171 1203
Size: 1749 2242 977 1327
Fragments arranged by size:
2242 1749 1327 977
AgeI A'CCGG_T
Cuts at: 5126 5126
Size: 6295
AhdI GACnn_n'nnGTC
Cuts at: 5024 5024
Size: 6295
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6295
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6295
AlwNI CAG_nnn'CTG
Cuts at: 4038 5610 4038
Size: 1572 4723
ApaLI G'TGCA_C
Cuts at: 4352 5508 4352
Size: 1156 5139
ApoI r'AATT_y
Cuts at: 1493 2280 2682 2801 3348 3536 3604 3830 3982
Size: 787 402 119 547 188 68 226 152
Cuts at: 3982 6091 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 787 547 402 226 188 152
119 68
AseI AT'TA_AT
Cuts at: 160 1032 3306 4849 160
Size: 872 2274 1543 1606
Fragments arranged by size:
2274 1606 1543 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
AvaI C'yCGr_G
Cuts at: 1870 1943 3620 1870
Size: 73 1677 4545
Fragments arranged by size:
4545 1677 73
BamHI G'GATC_C
Cuts at: 3168 3462 3625 3992 3168
Size: 294 163 367 5471
Fragments arranged by size:
5471 367 294 163
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3616 5071 618
Size: 325 217 627 1829 1455 1842
Fragments arranged by size:
1842 1829 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2903 3614 1391
Size: 1512 711 4072
Fragments arranged by size:
4072 1512 711
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2129 2513 2737 3382 928
Size: 971 230 384 224 645 3841
Fragments arranged by size:
3841 971 645 384 230 224
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4486 4520 1903
Size: 34 2549 34 3678
Fragments arranged by size:
3678 2549 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4187 5397 4187
Size: 1210 5085
BclI T'GATC_A
Cuts at: 1548 2614 2943 1548
Size: 1066 329 4900
Fragments arranged by size:
4900 1066 329
BfrBI ATG'CAT
Cuts at: 3290 3290
Size: 6295
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3509 4906 136
Size: 107 122 71 631 605 1837 1397 1525
Fragments arranged by size:
1837 1525 1397 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6290 6290
Size: 6295
BlpI GC'TnA_GC
Cuts at: 3712 3712
Size: 6295
Bme1580I G_kGCm'C
Cuts at: 1790 4356 5512 1790
Size: 2566 1156 2573
Fragments arranged by size:
2573 2566 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6295
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4026 4984 442
Size: 416 53 83 399 24 2609 958 1753
Fragments arranged by size:
2609 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6295
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3260 3299 3260
Size: 39 6256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2925 4955 1239
Size: 1686 2030 2579
Fragments arranged by size:
2579 2030 1686
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4420 5285 5583 4420
Size: 865 298 5132
Fragments arranged by size:
5132 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4958 882
Size: 617 3459 2219
Fragments arranged by size:
3459 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4086 493
Size: 711 2882 2702
Fragments arranged by size:
2882 2702 711
BsaBI GATnn'nnATC
Cuts at: 3524 6289 3524
Size: 2765 3530
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2021 4102 4484 5119
Size: 53 83 186 936 488 2081 382 635
Cuts at: 5119 275
Size: 1451
Fragments arranged by size:
2081 1451 936 635 488 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4728 5126 5400 5547 5958 4728
Size: 398 274 147 411 5065
Fragments arranged by size:
5065 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6070
Fragments arranged by size:
6070 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6295
BseYI C'CCAG_C
Cuts at: 5498 5994 5498
Size: 496 5799
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2915 1163
Size: 573 1179 4543
Fragments arranged by size:
4543 1179 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3745 4506 5534 1540
Size: 275 186 1744 761 1028 2301
Fragments arranged by size:
2301 1744 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2903 4356 4441 5512 2903
Size: 1453 85 1071 3686
Fragments arranged by size:
3686 1453 1071 85
BsmI GAATG_Cn'
Cuts at: 3183 3491 3806 3899 3183
Size: 308 315 93 5579
Fragments arranged by size:
5579 315 308 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2903 3614 4356 4441 5512 1391
Size: 399 1113 711 742 85 1071 2174
Fragments arranged by size:
2174 1113 1071 742 711 399 85
BspEI T'CCGG_A
Cuts at: 5958 5958
Size: 6295
BspHI T'CATG_A
Cuts at: 2155 3333 3355 4184 5914 2155
Size: 1178 22 829 1730 2536
Fragments arranged by size:
2536 1730 1178 829 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3650 844
Size: 2806 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4182 1394
Size: 429 2359 3507
Fragments arranged by size:
3507 2359 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3213 4790 4964 65
Size: 1183 1965 1577 174 1396
Fragments arranged by size:
1965 1577 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4939 5126 1700
Size: 110 3129 187 2869
Fragments arranged by size:
3129 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2514 3066 96
Size: 2418 552 3325
Fragments arranged by size:
3325 2418 552
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6295
BssSI C'ACGA_G
Cuts at: 4355 5367 4355
Size: 1012 5283
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6295
BstBI TT'CG_AA
Cuts at: 2782 5099 2782
Size: 2317 3978
BstEII G'GTnAC_C
Cuts at: 1184 3075 1184
Size: 1891 4404
BstYI r'GATC_y
Cuts at: 1972 2997 3168 3462 3625 3992 4377 4394 5846
Size: 1025 171 294 163 367 385 17 1452
Cuts at: 5846 5932 5944 6290 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1025 385 367 346 294 171
163 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2874 2874
Size: 6295
BtgI C'CryG_G
Cuts at: 513 1065 3163 513
Size: 552 2098 3645
Fragments arranged by size:
3645 2098 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5556
Fragments arranged by size:
5556 683 56
BtsI GCAGTG_nn'
Cuts at: 3811 4605 4625 3811
Size: 794 20 5481
Fragments arranged by size:
5481 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6295
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6260
DraI TTT'AAA
Cuts at: 3599 3599
Size: 6295
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5302 809
Size: 507 3986 1802
Fragments arranged by size:
3986 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3742 4631 6260
Size: 54 1425 50 275 18 1912 889 1629
Cuts at: 6260 8
Size: 43
Fragments arranged by size:
1912 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3742 1537
Size: 275 1930 4090
Fragments arranged by size:
4090 1930 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2130 2277 2880 4225 1353
Size: 777 147 603 1345 3423
Fragments arranged by size:
3423 1345 777 603 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4882 5256 5402 387
Size: 1064 138 3293 374 146 1280
Fragments arranged by size:
3293 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2901 2901
Size: 6295
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2714 2925 4358 4955 5742 6010 1239
Size: 393 1082 211 1433 597 787 268 1524
Fragments arranged by size:
1524 1433 1082 787 597 393 268 211
EcoNI CCTnn'n_nnAGG
Cuts at: 2873 2873
Size: 6295
EcoRI G'AATT_C
Cuts at: 3348 3604 3982 3348
Size: 256 378 5661
Fragments arranged by size:
5661 378 256
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2288 2288
Size: 6295
FspI TGC'GCA
Cuts at: 4801 4801
Size: 6295
HaeII r_GCGC'y
Cuts at: 1678 5442 1678
Size: 3764 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4936 4968 5010 5042 1373
Size: 32 135 32 3364 32 42 32 2626
Fragments arranged by size:
3364 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2172 3639 3885 669
Size: 988 281 234 1467 246 3079
Fragments arranged by size:
3079 1467 988 281 246 234
HindIII A'AGCT_T
Cuts at: 748 3114 3560 748
Size: 2366 446 3483
Fragments arranged by size:
3483 2366 446
HpaI GTT'AAC
Cuts at: 3885 3885
Size: 6295
KpnI G_GTAC'C
Cuts at: 3620 3620
Size: 6295
MfeI C'AATT_G
Cuts at: 3894 3894
Size: 6295
MluI A'CGCG_T
Cuts at: 6171 6171
Size: 6295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2818 5409 5593 1305
Size: 1513 2591 184 2007
Fragments arranged by size:
2591 2007 1513 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4473
Fragments arranged by size:
4473 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4253 4612 4771 518
Size: 648 3087 359 159 2042
Fragments arranged by size:
3087 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3711 4388 5536 5781 1606
Size: 2105 677 1148 245 2120
Fragments arranged by size:
2120 2105 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3163 513
Size: 552 2098 3645
Fragments arranged by size:
3645 2098 552
NdeI CA'TA_TG
Cuts at: 387 2655 387
Size: 2268 4027
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6295
NotI GC'GGCC_GC
Cuts at: 3742 3742
Size: 6295
NsiI A_TGCA'T
Cuts at: 3292 3292
Size: 6295
NspI r_CATG'y
Cuts at: 1132 1229 2956 3653 5198 1132
Size: 97 1727 697 1545 2229
Fragments arranged by size:
2229 1727 1545 697 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6295
PciI A'CATG_T
Cuts at: 2952 5194 2952
Size: 2242 4053
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3478 1108
Size: 2370 3925
PfoI T'CCnGG_A
Cuts at: 2130 2130
Size: 6295
PmeI GTTT'AAAC
Cuts at: 3599 3599
Size: 6295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4364 4396 5877 5909 4364
Size: 32 1481 32 4750
Fragments arranged by size:
4750 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2024 1613
Size: 411 5884
PsiI TTA'TAA
Cuts at: 3122 3865 3122
Size: 743 5552
PstI C_TGCA'G
Cuts at: 830 3647 830
Size: 2817 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6295
SacI G_AGCT'C
Cuts at: 2903 2903
Size: 6295
SalI G'TCGA_C
Cuts at: 1936 3637 1936
Size: 1701 4594
SapI GCTCTTCn'nnn_
Cuts at: 2130 2130
Size: 6295
SbfI CC_TGCA'GG
Cuts at: 3647 3647
Size: 6295
ScaI AGT'ACT
Cuts at: 4543 6255 4543
Size: 1712 4583
SfcI C'TryA_G
Cuts at: 826 1045 2371 3643 4778 5459 5650 826
Size: 219 1326 1272 1135 681 191 1471
Fragments arranged by size:
1471 1326 1272 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6295
SmaI CCC'GGG
Cuts at: 1872 3622 1872
Size: 1750 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4399 5300 5562 820
Size: 197 926 2456 901 262 1553
Fragments arranged by size:
2456 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4086 493
Size: 3593 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6295
SphI G_CATG'C
Cuts at: 1132 3653 1132
Size: 2521 3774
SspI AAT'ATT
Cuts at: 5 52 2363 4219 5
Size: 47 2311 1856 2081
Fragments arranged by size:
2311 2081 1856 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6295
StyI C'CwwG_G
Cuts at: 513 1065 3163 3231 3734 513
Size: 552 2098 68 503 3074
Fragments arranged by size:
3074 2098 552 503 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4322 4339 4492 4677 5122 325
Size: 1255 2742 17 153 185 445 1498
Fragments arranged by size:
2742 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2514 3066 4541
Size: 274 80 33 51 1443 537 552 1475
Cuts at: 4541 6253 96
Size: 1712 138
Fragments arranged by size:
1712 1475 1443 552 537 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2287 2319 6255 6287 2287
Size: 32 3936 32 2295
Fragments arranged by size:
3936 2295 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3265 3314 1316
Size: 1949 49 4297
Fragments arranged by size:
4297 1949 49
XbaI T'CTAG_A
Cuts at: 3631 3631
Size: 6295
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6295
XmaI C'CCGG_G
Cuts at: 1870 3620 1870
Size: 1750 4545
XmnI GAAnn'nnTTC
Cuts at: 4424 4424
Size: 6295
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2022 4103 276
Size: 53 83 186 936 488 2081 2468
Fragments arranged by size:
2468 2081 936 488 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI