Restriction map for
pFN21AE4506
(Circular) (Six-base) MAPSORT of: pFN21AE4506.seq Check: 9471 from: 1 to: 6292
pFN21AE4506 6292 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2445 4102 278
Size: 53 83 186 936 909 1657 2468
Fragments arranged by size:
2468 1657 936 909 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3635 1937
Size: 1698 4594
Acc65I G'GTAC_C
Cuts at: 3613 3613
Size: 6292
AclI AA'CG_TT
Cuts at: 1563 4419 4792 1563
Size: 2856 373 3063
Fragments arranged by size:
3063 2856 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2007 2163 4355 5739 6007 1632
Size: 375 156 2192 1384 268 1917
Fragments arranged by size:
2192 1917 1384 375 268 156
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6095
AflIII A'CryG_T
Cuts at: 1203 5191 6168 1203
Size: 3988 977 1327
Fragments arranged by size:
3988 1327 977
AgeI A'CCGG_T
Cuts at: 5123 5123
Size: 6292
AhdI GACnn_n'nnGTC
Cuts at: 5021 5021
Size: 6292
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6292
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6292
AlwNI CAG_nnn'CTG
Cuts at: 4035 5607 4035
Size: 1572 4720
ApaLI G'TGCA_C
Cuts at: 4349 5505 4349
Size: 1156 5136
ApoI r'AATT_y
Cuts at: 1493 2229 2266 2408 2791 3345 3398 3601 3827
Size: 736 37 142 383 554 53 203 226
Cuts at: 3827 3979 6088 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 736 554 383 226 203 152
142 53 37
AseI AT'TA_AT
Cuts at: 160 1032 3303 4846 160
Size: 872 2271 1543 1606
Fragments arranged by size:
2271 1606 1543 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
AvaI C'yCGr_G
Cuts at: 1870 1943 3617 1870
Size: 73 1674 4545
Fragments arranged by size:
4545 1674 73
BamHI G'GATC_C
Cuts at: 3165 3622 3989 3165
Size: 457 367 5468
Fragments arranged by size:
5468 457 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3613 5068 618
Size: 325 217 627 1826 1455 1842
Fragments arranged by size:
1842 1826 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2900 3611 1391
Size: 1509 711 4072
Fragments arranged by size:
4072 1509 711
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2734 2889 3379 928
Size: 971 835 155 490 3841
Fragments arranged by size:
3841 971 835 490 155
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4483 4517 1903
Size: 34 2546 34 3678
Fragments arranged by size:
3678 2546 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3066 4184 5394 3066
Size: 1118 1210 3964
Fragments arranged by size:
3964 1210 1118
BclI T'GATC_A
Cuts at: 1548 2611 3355 1548
Size: 1063 744 4485
Fragments arranged by size:
4485 1063 744
BfrBI ATG'CAT
Cuts at: 3287 3287
Size: 6292
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4903 136
Size: 107 122 71 631 605 3231 1525
Fragments arranged by size:
3231 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6287 6287
Size: 6292
BlpI GC'TnA_GC
Cuts at: 2487 3709 2487
Size: 1222 5070
Bme1580I G_kGCm'C
Cuts at: 1790 4353 5509 1790
Size: 2563 1156 2573
Fragments arranged by size:
2573 2563 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6292
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4023 4981 442
Size: 416 53 83 399 24 2606 958 1753
Fragments arranged by size:
2606 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2922 4952 1239
Size: 1683 2030 2579
Fragments arranged by size:
2579 2030 1683
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4417 5282 5580 4417
Size: 865 298 5129
Fragments arranged by size:
5129 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3053 4955 882
Size: 617 1554 1902 2219
Fragments arranged by size:
2219 1902 1554 617
BsaAI yAC'GTr
Cuts at: 493 1204 2584 4083 493
Size: 711 1380 1499 2702
Fragments arranged by size:
2702 1499 1380 711
BsaBI GATnn'nnATC
Cuts at: 3521 6286 3521
Size: 2765 3527
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2442 4099 4481 5116
Size: 53 83 186 936 909 1657 382 635
Cuts at: 5116 275
Size: 1451
Fragments arranged by size:
1657 1451 936 909 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4725 5123 5397 5544 5955 4725
Size: 398 274 147 411 5062
Fragments arranged by size:
5062 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3225 3255 1178
Size: 30 165 30 1822 30 4215
Fragments arranged by size:
4215 1822 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6292
BseYI C'CCAG_C
Cuts at: 5495 5991 5495
Size: 496 5796
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5719
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3742 4503 5531 1540
Size: 275 186 1741 761 1028 2301
Fragments arranged by size:
2301 1741 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2900 4353 4438 5509 2900
Size: 1453 85 1071 3683
Fragments arranged by size:
3683 1453 1071 85
BsmI GAATG_Cn'
Cuts at: 3289 3803 3896 3289
Size: 514 93 5685
Fragments arranged by size:
5685 514 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2900 3611 4353 4438 5509 1391
Size: 399 1110 711 742 85 1071 2174
Fragments arranged by size:
2174 1110 1071 742 711 399 85
BspEI T'CCGG_A
Cuts at: 5955 5955
Size: 6292
BspHI T'CATG_A
Cuts at: 2155 4181 5911 2155
Size: 2026 1730 2536
Fragments arranged by size:
2536 2026 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2863 3647 844
Size: 2019 784 3489
Fragments arranged by size:
3489 2019 784
BsrBI CCG'CTC
Cuts at: 1394 1823 2216 4179 1394
Size: 429 393 1963 3507
Fragments arranged by size:
3507 1963 429 393
BsrDI GCAATG_nn'
Cuts at: 65 1248 3210 4787 4961 65
Size: 1183 1962 1577 174 1396
Fragments arranged by size:
1962 1577 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4936 5123 1700
Size: 110 3126 187 2869
Fragments arranged by size:
3126 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2511 2890 96
Size: 2415 379 3498
Fragments arranged by size:
3498 2415 379
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6292
BssSI C'ACGA_G
Cuts at: 4352 5364 4352
Size: 1012 5280
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6292
BstBI TT'CG_AA
Cuts at: 2676 2779 5096 2676
Size: 103 2317 3872
Fragments arranged by size:
3872 2317 103
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6292
BstYI r'GATC_y
Cuts at: 1972 2994 3165 3622 3989 4374 4391 5843 5929
Size: 1022 171 457 367 385 17 1452 86
Cuts at: 5929 5941 6287 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1022 457 385 367 346 171
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2871 3043 2871
Size: 172 6120
BtgI C'CryG_G
Cuts at: 513 1065 3160 513
Size: 552 2095 3645
Fragments arranged by size:
3645 2095 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5553
Fragments arranged by size:
5553 683 56
BtsI GCAGTG_nn'
Cuts at: 2845 3808 4602 4622 2845
Size: 963 794 20 4515
Fragments arranged by size:
4515 963 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6292
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6257
DraI TTT'AAA
Cuts at: 3596 3596
Size: 6292
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5299 809
Size: 507 3983 1802
Fragments arranged by size:
3983 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3739 4628 6257
Size: 54 1425 50 275 18 1909 889 1629
Cuts at: 6257 8
Size: 43
Fragments arranged by size:
1909 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3739 1537
Size: 275 1927 4090
Fragments arranged by size:
4090 1927 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3287 4222 1353
Size: 1934 935 3423
Fragments arranged by size:
3423 1934 935
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4879 5253 5399 387
Size: 1064 138 3290 374 146 1280
Fragments arranged by size:
3290 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2898 2898
Size: 6292
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2007 2163 2922 4355 4952 5739 6007
Size: 393 375 156 759 1433 597 787 268
Cuts at: 6007 1239
Size: 1524
Fragments arranged by size:
1524 1433 787 759 597 393 375 268
156
EcoNI CCTnn'n_nnAGG
Cuts at: 2870 2870
Size: 6292
EcoRI G'AATT_C
Cuts at: 3345 3601 3979 3345
Size: 256 378 5658
Fragments arranged by size:
5658 378 256
FspI TGC'GCA
Cuts at: 4798 4798
Size: 6292
HaeII r_GCGC'y
Cuts at: 1678 5439 1678
Size: 3761 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2427 2459 4933 4965 5007
Size: 32 135 32 855 32 2474 32 42
Cuts at: 5007 5039 1373
Size: 32 2626
Fragments arranged by size:
2626 2474 855 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3636 3882 669
Size: 988 281 1698 246 3079
Fragments arranged by size:
3079 1698 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6292
HpaI GTT'AAC
Cuts at: 3882 3882
Size: 6292
KpnI G_GTAC'C
Cuts at: 3617 3617
Size: 6292
MfeI C'AATT_G
Cuts at: 3891 3891
Size: 6292
MluI A'CGCG_T
Cuts at: 6168 6168
Size: 6292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2241 2815 5406 5590 1305
Size: 936 574 2591 184 2007
Fragments arranged by size:
2591 2007 936 574 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4470
Fragments arranged by size:
4470 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 4250 4609 4768 518
Size: 648 843 2241 359 159 2042
Fragments arranged by size:
2241 2042 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3239 3708 4385 5533 5778 1606
Size: 1633 469 677 1148 245 2120
Fragments arranged by size:
2120 1633 1148 677 469 245
NcoI C'CATG_G
Cuts at: 513 1065 3160 513
Size: 552 2095 3645
Fragments arranged by size:
3645 2095 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6292
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6292
NotI GC'GGCC_GC
Cuts at: 3739 3739
Size: 6292
NsiI A_TGCA'T
Cuts at: 3289 3289
Size: 6292
NspI r_CATG'y
Cuts at: 1132 1229 3650 5195 1132
Size: 97 2421 1545 2229
Fragments arranged by size:
2421 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6292
PciI A'CATG_T
Cuts at: 5191 5191
Size: 6292
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3475 1108
Size: 2367 3925
PfoI T'CCnGG_A
Cuts at: 2130 2786 2130
Size: 656 5636
PmeI GTTT'AAAC
Cuts at: 3596 3596
Size: 6292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4361 4393 5874 5906 4361
Size: 32 1481 32 4747
Fragments arranged by size:
4747 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6292
PsiI TTA'TAA
Cuts at: 3119 3862 3119
Size: 743 5549
PstI C_TGCA'G
Cuts at: 830 3644 830
Size: 2814 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6292
PvuII CAG'CTG
Cuts at: 3239 3239
Size: 6292
SacI G_AGCT'C
Cuts at: 2900 2900
Size: 6292
SalI G'TCGA_C
Cuts at: 1936 3634 1936
Size: 1698 4594
SbfI CC_TGCA'GG
Cuts at: 3644 3644
Size: 6292
ScaI AGT'ACT
Cuts at: 4540 6252 4540
Size: 1712 4580
SexAI A'CCwGG_T
Cuts at: 3455 3455
Size: 6292
SfcI C'TryA_G
Cuts at: 826 1045 2470 3640 4775 5456 5647 826
Size: 219 1425 1170 1135 681 191 1471
Fragments arranged by size:
1471 1425 1170 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6292
SmaI CCC'GGG
Cuts at: 1872 3619 1872
Size: 1747 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4396 5297 5559 820
Size: 197 926 2453 901 262 1553
Fragments arranged by size:
2453 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2584 4083 493
Size: 2091 1499 2702
Fragments arranged by size:
2702 2091 1499
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6292
SphI G_CATG'C
Cuts at: 1132 3650 1132
Size: 2518 3774
SspI AAT'ATT
Cuts at: 5 52 2358 2682 4216 5
Size: 47 2306 324 1534 2081
Fragments arranged by size:
2306 2081 1534 324 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6292
StyI C'CwwG_G
Cuts at: 513 1065 3160 3731 513
Size: 552 2095 571 3074
Fragments arranged by size:
3074 2095 571 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4319 4336 4489 4674 5119 325
Size: 1255 2739 17 153 185 445 1498
Fragments arranged by size:
2739 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2167 2338 2422
Size: 274 80 33 51 1443 190 171 84
Cuts at: 2422 2511 2890 3269 4538 6250 96
Size: 89 379 379 1269 1712 138
Fragments arranged by size:
1712 1443 1269 379 379 274 190 171
138 89 84 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6252 6284 6252
Size: 32 6260
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6292
XbaI T'CTAG_A
Cuts at: 3628 3628
Size: 6292
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6292
XmaI C'CCGG_G
Cuts at: 1870 3617 1870
Size: 1747 4545
XmnI GAAnn'nnTTC
Cuts at: 4421 4421
Size: 6292
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2443 4100 276
Size: 53 83 186 936 909 1657 2468
Fragments arranged by size:
2468 1657 936 909 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI