Restriction map for
pFN21AE4501
(Circular) (Six-base) MAPSORT of: pFN21AE4501.seq Check: 7867 from: 1 to: 6286
pFN21AE4501 6286 bp
With 182 enzymes: SgfI *
March 28, 2012 14:05 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4096 278
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3629 1937
Size: 1692 4594
Acc65I G'GTAC_C
Cuts at: 3607 3607
Size: 6286
AclI AA'CG_TT
Cuts at: 1563 4413 4786 1563
Size: 2850 373 3063
Fragments arranged by size:
3063 2850 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2836 3530 4349 5733 6001 1632
Size: 1204 694 819 1384 268 1917
Fragments arranged by size:
1917 1384 1204 819 694 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6089
AflIII A'CryG_T
Cuts at: 1203 2040 5185 6162 1203
Size: 837 3145 977 1327
Fragments arranged by size:
3145 1327 977 837
AgeI A'CCGG_T
Cuts at: 5117 5117
Size: 6286
AhdI GACnn_n'nnGTC
Cuts at: 5015 5015
Size: 6286
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6286
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6286
AlwNI CAG_nnn'CTG
Cuts at: 3314 3539 4029 5601 3314
Size: 225 490 1572 3999
Fragments arranged by size:
3999 1572 490 225
ApaLI G'TGCA_C
Cuts at: 4343 5499 4343
Size: 1156 5130
ApoI r'AATT_y
Cuts at: 1493 3595 3821 3973 6082 1493
Size: 2102 226 152 2109 1697
Fragments arranged by size:
2109 2102 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2814 4840 160
Size: 872 1782 2026 1606
Fragments arranged by size:
2026 1782 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
AvaI C'yCGr_G
Cuts at: 1870 1943 2136 3392 3611 1870
Size: 73 193 1256 219 4545
Fragments arranged by size:
4545 1256 219 193 73
BamHI G'GATC_C
Cuts at: 3149 3616 3983 3149
Size: 467 367 5452
Fragments arranged by size:
5452 467 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2333 2612 3607 5062 618
Size: 325 217 627 546 279 995 1455 1842
Fragments arranged by size:
1842 1455 995 627 546 325 279 217
BanII G_rGCy'C
Cuts at: 1391 2484 3132 3605 1391
Size: 1093 648 473 4072
Fragments arranged by size:
4072 1093 648 473
BbeI G_GCGC'C
Cuts at: 2337 2337
Size: 6286
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5315
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2555 2589 4477 4511 1903
Size: 34 618 34 1888 34 3678
Fragments arranged by size:
3678 1888 618 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4178 5388 4178
Size: 1210 5076
BclI T'GATC_A
Cuts at: 1548 2976 1548
Size: 1428 4858
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4897 136
Size: 107 122 71 631 605 3225 1525
Fragments arranged by size:
3225 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6281 6281
Size: 6286
BlpI GC'TnA_GC
Cuts at: 3090 3703 3090
Size: 613 5673
Bme1580I G_kGCm'C
Cuts at: 1790 2615 3308 4347 5503 1790
Size: 825 693 1039 1156 2573
Fragments arranged by size:
2573 1156 1039 825 693
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6286
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4017 4975 442
Size: 416 53 83 399 24 2600 958 1753
Fragments arranged by size:
2600 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6286
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3226 3226
Size: 6286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3378 4946 1239
Size: 2139 1568 2579
Fragments arranged by size:
2579 2139 1568
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4411 5276 5574 4411
Size: 865 298 5123
Fragments arranged by size:
5123 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2703 3395 4949 882
Size: 617 1204 692 1554 2219
Fragments arranged by size:
2219 1554 1204 692 617
BsaAI yAC'GTr
Cuts at: 493 1204 2191 2809 4077 493
Size: 711 987 618 1268 2702
Fragments arranged by size:
2702 1268 987 711 618
BsaBI GATnn'nnATC
Cuts at: 6280 6280
Size: 6286
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2334 4093 4475 5110
Size: 53 83 186 936 801 1759 382 635
Cuts at: 5110 275
Size: 1451
Fragments arranged by size:
1759 1451 936 801 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4719 5117 5391 5538 5949 4719
Size: 398 274 147 411 5056
Fragments arranged by size:
5056 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2196 2226 2700 2730 1178
Size: 30 165 30 793 30 474 30 4734
Fragments arranged by size:
4734 793 474 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2693 3215 1186
Size: 1507 522 4257
Fragments arranged by size:
4257 1507 522
BseYI C'CCAG_C
Cuts at: 2254 3145 5489 5985 2254
Size: 891 2344 496 2555
Fragments arranged by size:
2555 2344 891 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5713
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3736 4497 5525 1540
Size: 275 186 1735 761 1028 2301
Fragments arranged by size:
2301 1735 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2484 3132 4347 4432 5503 2484
Size: 648 1215 85 1071 3267
Fragments arranged by size:
3267 1215 1071 648 85
BsmI GAATG_Cn'
Cuts at: 3797 3890 3797
Size: 93 6193
BsmBI CGTCTCn'nnnn_
Cuts at: 2297 2768 2297
Size: 471 5815
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2484 2615 3132 3308 3605 4347 4432
Size: 399 694 131 517 176 297 742 85
Cuts at: 4432 5503 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 694 517 399 297 176
131 85
BspEI T'CCGG_A
Cuts at: 5949 5949
Size: 6286
BspHI T'CATG_A
Cuts at: 3249 4175 5905 3249
Size: 926 1730 3630
Fragments arranged by size:
3630 1730 926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3495 3641 844
Size: 2651 146 3489
Fragments arranged by size:
3489 2651 146
BsrBI CCG'CTC
Cuts at: 1394 1823 3330 4173 1394
Size: 429 1507 843 3507
Fragments arranged by size:
3507 1507 843 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2358 4781 4955 65
Size: 1183 1110 2423 174 1396
Fragments arranged by size:
2423 1396 1183 1110 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4930 5117 1700
Size: 110 3120 187 2869
Fragments arranged by size:
3120 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3558 96
Size: 3462 2824
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6286
BssSI C'ACGA_G
Cuts at: 4346 5358 4346
Size: 1012 5274
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6286
BstBI TT'CG_AA
Cuts at: 5090 5090
Size: 6286
BstEII G'GTnAC_C
Cuts at: 1184 3400 1184
Size: 2216 4070
BstXI CCAn_nnnn'nTGG
Cuts at: 2349 3145 2349
Size: 796 5490
BstYI r'GATC_y
Cuts at: 1972 2669 3149 3616 3983 4368 4385 5837 5923
Size: 697 480 467 367 385 17 1452 86
Cuts at: 5923 5935 6281 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 697 480 467 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3282 3578 513
Size: 552 939 1278 296 3221
Fragments arranged by size:
3221 1278 939 552 296
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5547
Fragments arranged by size:
5547 683 56
BtsI GCAGTG_nn'
Cuts at: 2609 3038 3802 4596 4616 2609
Size: 429 764 794 20 4279
Fragments arranged by size:
4279 794 764 429 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6286
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6251
DraI TTT'AAA
Cuts at: 3590 3590
Size: 6286
DraIII CAC_nnn'GTG
Cuts at: 2569 3324 2569
Size: 755 5531
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5293 809
Size: 507 3977 1802
Fragments arranged by size:
3977 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2644 3733 4622
Size: 54 1425 50 275 18 814 1089 889
Cuts at: 4622 6251 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1089 889 814 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3733 1537
Size: 275 1921 4090
Fragments arranged by size:
4090 1921 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3177 4216 1353
Size: 1824 1039 3423
Fragments arranged by size:
3423 1824 1039
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4873 5247 5393 387
Size: 1064 138 3284 374 146 1280
Fragments arranged by size:
3284 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2482 3130 2482
Size: 648 5638
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2836 3378 3530 4349 4946 5733 6001
Size: 393 1204 542 152 819 597 787 268
Cuts at: 6001 1239
Size: 1524
Fragments arranged by size:
1524 1204 819 787 597 542 393 268
152
EcoRI G'AATT_C
Cuts at: 3595 3973 3595
Size: 378 5908
EcoRV GAT'ATC
Cuts at: 3415 3415
Size: 6286
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2866 2959 2866
Size: 93 6193
FspI TGC'GCA
Cuts at: 4792 4792
Size: 6286
HaeII r_GCGC'y
Cuts at: 1678 2337 5433 1678
Size: 659 3096 2531
Fragments arranged by size:
3096 2531 659
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3238 3270 4927 4959 5001
Size: 32 135 32 1666 32 1657 32 42
Cuts at: 5001 5033 1373
Size: 32 2626
Fragments arranged by size:
2626 1666 1657 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2746 3278 3630 3876 669
Size: 988 281 808 532 352 246 3079
Fragments arranged by size:
3079 988 808 532 352 281 246
HindIII A'AGCT_T
Cuts at: 748 3159 748
Size: 2411 3875
HpaI GTT'AAC
Cuts at: 3876 3876
Size: 6286
KasI G'GCGC_C
Cuts at: 2333 2333
Size: 6286
KpnI G_GTAC'C
Cuts at: 3611 3611
Size: 6286
MfeI C'AATT_G
Cuts at: 3885 3885
Size: 6286
MluI A'CGCG_T
Cuts at: 6162 6162
Size: 6286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2128 2444 2852 5400 5584 1305
Size: 823 316 408 2548 184 2007
Fragments arranged by size:
2548 2007 823 408 316 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2646 10
Size: 54 1425 343 814 3650
Fragments arranged by size:
3650 1425 814 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2347 4244 4603 4762 518
Size: 648 1181 1897 359 159 2042
Fragments arranged by size:
2042 1897 1181 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2893 3452 3702 4379 5527 5772 1606
Size: 1287 559 250 677 1148 245 2120
Fragments arranged by size:
2120 1287 1148 677 559 250 245
NarI GG'CG_CC
Cuts at: 2334 2334
Size: 6286
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4795
Fragments arranged by size:
4795 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6286
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6286
NotI GC'GGCC_GC
Cuts at: 3733 3733
Size: 6286
NspI r_CATG'y
Cuts at: 1132 1229 2044 2183 2450 3644 5189 1132
Size: 97 815 139 267 1194 1545 2229
Fragments arranged by size:
2229 1545 1194 815 267 139 97
PasI CC'CwG_GG
Cuts at: 1348 2433 3544 1348
Size: 1085 1111 4090
Fragments arranged by size:
4090 1111 1085
PciI A'CATG_T
Cuts at: 2040 5185 2040
Size: 3145 3141
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3324 1108
Size: 2216 4070
PmeI GTTT'AAAC
Cuts at: 3590 3590
Size: 6286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3476 3508 4355 4387 5868 5900 3476
Size: 32 847 32 1481 32 3862
Fragments arranged by size:
3862 1481 847 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6286
PsiI TTA'TAA
Cuts at: 3856 3856
Size: 6286
PstI C_TGCA'G
Cuts at: 830 3638 830
Size: 2808 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6286
SacI G_AGCT'C
Cuts at: 2484 3132 2484
Size: 648 5638
SalI G'TCGA_C
Cuts at: 1936 3628 1936
Size: 1692 4594
SbfI CC_TGCA'GG
Cuts at: 3638 3638
Size: 6286
ScaI AGT'ACT
Cuts at: 3209 4534 6246 3209
Size: 1325 1712 3249
Fragments arranged by size:
3249 1712 1325
SfcI C'TryA_G
Cuts at: 826 1045 3634 4769 5450 5641 826
Size: 219 2589 1135 681 191 1471
Fragments arranged by size:
2589 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2335 2335
Size: 6286
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6286
SmaI CCC'GGG
Cuts at: 1872 3613 1872
Size: 1741 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4390 5291 5553 820
Size: 197 926 2447 901 262 1553
Fragments arranged by size:
2447 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2191 2809 4077 493
Size: 1698 618 1268 2702
Fragments arranged by size:
2702 1698 1268 618
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6286
SphI G_CATG'C
Cuts at: 1132 3644 1132
Size: 2512 3774
SspI AAT'ATT
Cuts at: 5 52 4210 5
Size: 47 4158 2081
Fragments arranged by size:
4158 2081 47
StuI AGG'CCT
Cuts at: 1517 2942 1517
Size: 1425 4861
StyI C'CwwG_G
Cuts at: 513 1065 2004 3012 3725 513
Size: 552 939 1008 713 3074
Fragments arranged by size:
3074 1008 939 713 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3419 3479 4313 4330 4483 4668 5113
Size: 1255 1839 60 834 17 153 185 445
Cuts at: 5113 325
Size: 1498
Fragments arranged by size:
1839 1498 1255 834 445 185 153 60
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3207 3558 4532
Size: 274 80 33 51 1443 1230 351 974
Cuts at: 4532 6244 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1230 974 351 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2196 2228 2700 2732 6246 6278 2196
Size: 32 472 32 3514 32 2204
Fragments arranged by size:
3514 2204 472 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2051 1316
Size: 735 5551
XbaI T'CTAG_A
Cuts at: 3622 3622
Size: 6286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2365 2365
Size: 6286
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6286
XmaI C'CCGG_G
Cuts at: 1870 3611 1870
Size: 1741 4545
XmnI GAAnn'nnTTC
Cuts at: 4415 4415
Size: 6286
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4094 276
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoO109I FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI