Restriction map for
pFN21AE4495
(Circular) (Six-base) MAPSORT of: pFN21AE4495.seq Check: 8447 from: 1 to: 6262
pFN21AE4495 6262 bp
With 182 enzymes: SgfI *
March 28, 2012 13:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4072 278
Size: 53 83 186 936 2536 2468
Fragments arranged by size:
2536 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3605 1937
Size: 1668 4594
Acc65I G'GTAC_C
Cuts at: 2667 3583 2667
Size: 916 5346
AclI AA'CG_TT
Cuts at: 1563 4389 4762 1563
Size: 2826 373 3063
Fragments arranged by size:
3063 2826 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3022 4325 5709 5977 1632
Size: 1390 1303 1384 268 1917
Fragments arranged by size:
1917 1390 1384 1303 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6065
AflIII A'CryG_T
Cuts at: 1203 5161 6138 1203
Size: 3958 977 1327
Fragments arranged by size:
3958 1327 977
AgeI A'CCGG_T
Cuts at: 2345 2664 5093 2345
Size: 319 2429 3514
Fragments arranged by size:
3514 2429 319
AhdI GACnn_n'nnGTC
Cuts at: 4991 4991
Size: 6262
AleI CACnn'nnGTG
Cuts at: 1166 2192 1166
Size: 1026 5236
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2072 2210 2975 1184
Size: 888 138 765 4471
Fragments arranged by size:
4471 888 765 138
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2381 2413 2381
Size: 32 6230
AlwNI CAG_nnn'CTG
Cuts at: 3265 4005 5577 3265
Size: 740 1572 3950
Fragments arranged by size:
3950 1572 740
ApaI G_GGCC'C
Cuts at: 2511 2511
Size: 6262
ApaLI G'TGCA_C
Cuts at: 2185 2293 4319 5475 2185
Size: 108 2026 1156 2972
Fragments arranged by size:
2972 2026 1156 108
ApoI r'AATT_y
Cuts at: 1493 2270 3571 3797 3949 6058 1493
Size: 777 1301 226 152 2109 1697
Fragments arranged by size:
2109 1697 1301 777 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4816 160
Size: 872 3784 1606
Fragments arranged by size:
3784 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6262
AvaI C'yCGr_G
Cuts at: 1870 1943 2082 3587 1870
Size: 73 139 1505 4545
Fragments arranged by size:
4545 1505 139 73
BamHI G'GATC_C
Cuts at: 3592 3959 3592
Size: 367 5895
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2432 2661 2667 3583 5038
Size: 325 217 627 645 229 6 916 1455
Cuts at: 5038 618
Size: 1842
Fragments arranged by size:
1842 1455 916 645 627 325 229 217
6
BanII G_rGCy'C
Cuts at: 1391 2012 2068 2511 3581 1391
Size: 621 56 443 1070 4072
Fragments arranged by size:
4072 1070 621 443 56
BbeI G_GCGC'C
Cuts at: 2436 2436
Size: 6262
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3499 928
Size: 971 1600 3691
Fragments arranged by size:
3691 1600 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4453 4487 1903
Size: 34 2516 34 3678
Fragments arranged by size:
3678 2516 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4154 5364 4154
Size: 1210 5052
BclI T'GATC_A
Cuts at: 1548 2475 1548
Size: 927 5335
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4873 136
Size: 107 122 71 631 605 3201 1525
Fragments arranged by size:
3201 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2440 2692 6257 2440
Size: 252 3565 2445
Fragments arranged by size:
3565 2445 252
BlpI GC'TnA_GC
Cuts at: 3679 3679
Size: 6262
Bme1580I G_kGCm'C
Cuts at: 1790 2189 2297 2511 4323 5479 1790
Size: 399 108 214 1812 1156 2573
Fragments arranged by size:
2573 1812 1156 399 214 108
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6262
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2760 3993 4951
Size: 416 53 83 399 24 1343 1233 958
Cuts at: 4951 442
Size: 1753
Fragments arranged by size:
1753 1343 1233 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6262
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2236 2681 2827 2981 3300 3387 4922 1239
Size: 997 445 146 154 319 87 1535 2579
Fragments arranged by size:
2579 1535 997 445 319 154 146 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2422 2818 3078 4387 5252 5550 2422
Size: 396 260 1309 865 298 3134
Fragments arranged by size:
3134 1309 865 396 298 260
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2794 4925 882
Size: 617 1295 2131 2219
Fragments arranged by size:
2219 2131 1295 617
BsaAI yAC'GTr
Cuts at: 493 1204 2473 4053 493
Size: 711 1269 1580 2702
Fragments arranged by size:
2702 1580 1269 711
BsaBI GATnn'nnATC
Cuts at: 6256 6256
Size: 6262
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2433 2608 4069 4451
Size: 53 83 186 936 900 175 1461 382
Cuts at: 4451 5086 275
Size: 635 1451
Fragments arranged by size:
1461 1451 936 900 635 382 186 175
83 53
BsaWI w'CCGG_w
Cuts at: 2280 2345 2664 4695 5093 5367 5514 5925 2280
Size: 65 319 2031 398 274 147 411 2617
Fragments arranged by size:
2617 2031 411 398 319 274 147 65
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6037
Fragments arranged by size:
6037 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6262
BseYI C'CCAG_C
Cuts at: 2494 2520 2847 5465 5961 2494
Size: 26 327 2618 496 2795
Fragments arranged by size:
2795 2618 496 327 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2160 1163
Size: 573 424 5265
Fragments arranged by size:
5265 573 424
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3712 4473 5501 1540
Size: 275 186 1711 761 1028 2301
Fragments arranged by size:
2301 1711 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2189 2297 4323 4408 5479 2189
Size: 108 2026 85 1071 2972
Fragments arranged by size:
2972 2026 1071 108 85
BsmI GAATG_Cn'
Cuts at: 3055 3773 3866 3055
Size: 718 93 5451
Fragments arranged by size:
5451 718 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2068 2189 2297 2511 3581 4323
Size: 399 222 56 121 108 214 1070 742
Cuts at: 4323 4408 5479 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1070 742 399 222 214 121
108 85 56
BspEI T'CCGG_A
Cuts at: 5925 5925
Size: 6262
BspHI T'CATG_A
Cuts at: 4151 5881 4151
Size: 1730 4532
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2294 2653 3617 844
Size: 1450 359 964 3489
Fragments arranged by size:
3489 1450 964 359
BsrBI CCG'CTC
Cuts at: 1394 1823 2362 4149 1394
Size: 429 539 1787 3507
Fragments arranged by size:
3507 1787 539 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2719 2908 4757 4931 65
Size: 1183 1471 189 1849 174 1396
Fragments arranged by size:
1849 1471 1396 1183 189 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2309 2345 2664 4906 5093 1700
Size: 110 499 36 319 2242 187 2869
Fragments arranged by size:
2869 2242 499 319 187 110 36
BsrGI T'GTAC_A
Cuts at: 96 2774 3291 96
Size: 2678 517 3067
Fragments arranged by size:
3067 2678 517
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6262
BssSI C'ACGA_G
Cuts at: 4322 5334 4322
Size: 1012 5250
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6262
BstBI TT'CG_AA
Cuts at: 3499 5066 3499
Size: 1567 4695
BstEII G'GTnAC_C
Cuts at: 1184 2890 1184
Size: 1706 4556
BstXI CCAn_nnnn'nTGG
Cuts at: 2789 3181 2789
Size: 392 5870
BstYI r'GATC_y
Cuts at: 1972 2440 2692 3592 3959 4344 4361 5813 5899
Size: 468 252 900 367 385 17 1452 86
Cuts at: 5899 5911 6257 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 900 468 385 367 346 252
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2409 3258 2409
Size: 849 5413
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5710
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5523
Fragments arranged by size:
5523 683 56
BtsI GCAGTG_nn'
Cuts at: 2189 2548 3778 4572 4592 2189
Size: 359 1230 794 20 3859
Fragments arranged by size:
3859 1230 794 359 20
ClaI AT'CG_AT
Cuts at: 1554 3375 1554
Size: 1821 4441
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2140 2175 529
Size: 35 1576 35 4616
Fragments arranged by size:
4616 1576 35 35
DraI TTT'AAA
Cuts at: 3566 3566
Size: 6262
DraIII CAC_nnn'GTG
Cuts at: 2898 2898
Size: 6262
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5269 809
Size: 507 3953 1802
Fragments arranged by size:
3953 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 2261 3709
Size: 54 1425 50 275 18 318 113 1448
Cuts at: 3709 4598 6227 8
Size: 889 1629 43
Fragments arranged by size:
1629 1448 1425 889 318 275 113 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3709 1537
Size: 275 1897 4090
Fragments arranged by size:
4090 1897 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2447 4192 1353
Size: 1094 1745 3423
Fragments arranged by size:
3423 1745 1094
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4849 5223 5369 387
Size: 1064 138 3260 374 146 1280
Fragments arranged by size:
3260 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2236 2681 2827 2981 3022 3300 3387
Size: 393 604 445 146 154 41 278 87
Cuts at: 3387 4325 4922 5709 5977 1239
Size: 938 597 787 268 1524
Fragments arranged by size:
1524 938 787 604 597 445 393 278
268 154 146 87 41
EcoO109I rG'GnC_Cy
Cuts at: 2211 2642 2211
Size: 431 5831
EcoRI G'AATT_C
Cuts at: 3571 3949 3571
Size: 378 5884
FspI TGC'GCA
Cuts at: 4768 4768
Size: 6262
HaeII r_GCGC'y
Cuts at: 1678 2436 5409 1678
Size: 758 2973 2531
Fragments arranged by size:
2973 2531 758
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2917 2949 2975 3007 4903
Size: 32 135 32 1345 32 26 32 1896
Cuts at: 4903 4935 4977 5009 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1896 1345 135 42 32 32 32
32 32 32 26
HincII GTy'rAC
Cuts at: 669 1657 1938 3606 3852 669
Size: 988 281 1668 246 3079
Fragments arranged by size:
3079 1668 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6262
HpaI GTT'AAC
Cuts at: 3852 3852
Size: 6262
KasI G'GCGC_C
Cuts at: 2432 2432
Size: 6262
KpnI G_GTAC'C
Cuts at: 2671 3587 2671
Size: 916 5346
MfeI C'AATT_G
Cuts at: 3861 3861
Size: 6262
MluI A'CGCG_T
Cuts at: 6138 6138
Size: 6262
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2807 2837 5376 5560 1305
Size: 1502 30 2539 184 2007
Fragments arranged by size:
2539 2007 1502 184 30
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2263 10
Size: 54 1425 343 431 4009
Fragments arranged by size:
4009 1425 431 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2096 2192 3179 4220 4579 4738 518
Size: 648 930 96 987 1041 359 159 2042
Fragments arranged by size:
2042 1041 987 930 648 359 159 96
MspA1I CmG'CkG
Cuts at: 1606 2785 2847 3678 4355 5503 5748 1606
Size: 1179 62 831 677 1148 245 2120
Fragments arranged by size:
2120 1179 1148 831 677 245 62
NaeI GCC'GGC
Cuts at: 2311 2311
Size: 6262
NarI GG'CG_CC
Cuts at: 2433 2433
Size: 6262
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5710
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6262
NgoMIV G'CCGG_C
Cuts at: 2309 2309
Size: 6262
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6262
NotI GC'GGCC_GC
Cuts at: 3709 3709
Size: 6262
NruI TCG'CGA
Cuts at: 2447 2447
Size: 6262
NspI r_CATG'y
Cuts at: 1132 1229 3620 5165 1132
Size: 97 2391 1545 2229
Fragments arranged by size:
2391 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2068 1348
Size: 720 5542
PciI A'CATG_T
Cuts at: 5161 5161
Size: 6262
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6262
PmeI GTTT'AAAC
Cuts at: 3566 3566
Size: 6262
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4331 4363 5844 5876 4331
Size: 32 1481 32 4717
Fragments arranged by size:
4717 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2642 2642
Size: 6262
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6262
PsiI TTA'TAA
Cuts at: 3832 3832
Size: 6262
PspOMI G'GGCC_C
Cuts at: 2507 2507
Size: 6262
PstI C_TGCA'G
Cuts at: 830 2543 3247 3614 830
Size: 1713 704 367 3478
Fragments arranged by size:
3478 1713 704 367
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6262
PvuII CAG'CTG
Cuts at: 2785 2847 2785
Size: 62 6200
SalI G'TCGA_C
Cuts at: 1936 3604 1936
Size: 1668 4594
SbfI CC_TGCA'GG
Cuts at: 3614 3614
Size: 6262
ScaI AGT'ACT
Cuts at: 4510 6222 4510
Size: 1712 4550
SexAI A'CCwGG_T
Cuts at: 2886 2886
Size: 6262
SfcI C'TryA_G
Cuts at: 826 1045 2539 2614 3243 3361 3610 4745 5426
Size: 219 1494 75 629 118 249 1135 681
Cuts at: 5426 5617 826
Size: 191 1471
Fragments arranged by size:
1494 1471 1135 681 629 249 219 191
118 75
SfoI GGC'GCC
Cuts at: 2434 2434
Size: 6262
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6262
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6262
SmaI CCC'GGG
Cuts at: 1872 3589 1872
Size: 1717 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2401 2833 3093 4366 5267 5529
Size: 197 926 458 432 260 1273 901 262
Cuts at: 5529 820
Size: 1553
Fragments arranged by size:
1553 1273 926 901 458 432 262 260
197
SnaBI TAC'GTA
Cuts at: 493 4053 493
Size: 3560 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6262
SphI G_CATG'C
Cuts at: 1132 3620 1132
Size: 2488 3774
SspI AAT'ATT
Cuts at: 5 52 4186 5
Size: 47 4134 2081
Fragments arranged by size:
4134 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6262
StyI C'CwwG_G
Cuts at: 513 1065 3701 513
Size: 552 2636 3074
Fragments arranged by size:
3074 2636 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2126 4289 4306 4459 4644 5089 325
Size: 1255 546 2163 17 153 185 445 1498
Fragments arranged by size:
2163 1498 1255 546 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2458 2774 3291
Size: 274 80 33 51 1443 481 316 517
Cuts at: 3291 4508 6220 96
Size: 1217 1712 138
Fragments arranged by size:
1712 1443 1217 517 481 316 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2749 2781 3429 3461 6222 6254 2749
Size: 32 648 32 2761 32 2757
Fragments arranged by size:
2761 2757 648 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6262
XbaI T'CTAG_A
Cuts at: 3598 3598
Size: 6262
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2705 2705
Size: 6262
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6262
XmaI C'CCGG_G
Cuts at: 1870 3587 1870
Size: 1717 4545
XmnI GAAnn'nnTTC
Cuts at: 4391 4391
Size: 6262
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4070 276
Size: 53 83 186 936 2536 2468
Fragments arranged by size:
2536 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI
FseI FspAI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SrfI SwaI