Restriction map for
pFN21AE4494
(Circular) (Six-base) MAPSORT of: pFN21AE4494.seq Check: 9814 from: 1 to: 6250
pFN21AE4494 6250 bp
With 182 enzymes: SgfI *
March 28, 2012 13:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2005 2005
Size: 6250
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2856 3336 4060 278
Size: 53 83 186 936 1320 480 724 2468
Fragments arranged by size:
2468 1320 936 724 480 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2442 3593 1937
Size: 505 1151 4594
Fragments arranged by size:
4594 1151 505
Acc65I G'GTAC_C
Cuts at: 2967 3571 2967
Size: 604 5646
AclI AA'CG_TT
Cuts at: 1563 4377 4750 1563
Size: 2814 373 3063
Fragments arranged by size:
3063 2814 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4313 5697 5965 1632
Size: 2681 1384 268 1917
Fragments arranged by size:
2681 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6053
AflIII A'CryG_T
Cuts at: 1203 5149 6126 1203
Size: 3946 977 1327
Fragments arranged by size:
3946 1327 977
AgeI A'CCGG_T
Cuts at: 5081 5081
Size: 6250
AhdI GACnn_n'nnGTC
Cuts at: 2551 4979 2551
Size: 2428 3822
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6250
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6250
AlwNI CAG_nnn'CTG
Cuts at: 2237 2806 3993 5565 2237
Size: 569 1187 1572 2922
Fragments arranged by size:
2922 1572 1187 569
ApaLI G'TGCA_C
Cuts at: 4307 5463 4307
Size: 1156 5094
ApoI r'AATT_y
Cuts at: 1493 2759 3559 3785 3937 6046 1493
Size: 1266 800 226 152 2109 1697
Fragments arranged by size:
2109 1697 1266 800 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4804 160
Size: 872 3772 1606
Fragments arranged by size:
3772 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6250
AvaI C'yCGr_G
Cuts at: 1870 1943 3248 3575 1870
Size: 73 1305 327 4545
Fragments arranged by size:
4545 1305 327 73
BamHI G'GATC_C
Cuts at: 2046 3580 3947 2046
Size: 1534 367 4349
Fragments arranged by size:
4349 1534 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2967 2986 3058 3518 3571
Size: 325 217 627 1180 19 72 460 53
Cuts at: 3571 5026 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1180 627 460 325 217 72
53 19
BanII G_rGCy'C
Cuts at: 1391 2262 2543 2724 3378 3569 1391
Size: 871 281 181 654 191 4072
Fragments arranged by size:
4072 871 654 281 191 181
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3328 928
Size: 971 1429 3850
Fragments arranged by size:
3850 1429 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4441 4475 1903
Size: 34 2504 34 3678
Fragments arranged by size:
3678 2504 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2086 4142 5352 2086
Size: 2056 1210 2984
Fragments arranged by size:
2984 2056 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6250
BfrBI ATG'CAT
Cuts at: 2886 2886
Size: 6250
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2490 4861 136
Size: 107 122 71 631 605 818 2371 1525
Fragments arranged by size:
2371 1525 818 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2145 2589 6245 2145
Size: 444 3656 2150
Fragments arranged by size:
3656 2150 444
BlpI GC'TnA_GC
Cuts at: 3667 3667
Size: 6250
Bme1580I G_kGCm'C
Cuts at: 1790 2991 3063 3521 4311 5467 1790
Size: 1201 72 458 790 1156 2573
Fragments arranged by size:
2573 1201 1156 790 458 72
BmgBI CAC'GTC
Cuts at: 1318 2779 1318
Size: 1461 4789
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3981 4939 442
Size: 416 53 83 399 24 2564 958 1753
Fragments arranged by size:
2564 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6250
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2318 3446 4910 1239
Size: 1079 1128 1464 2579
Fragments arranged by size:
2579 1464 1128 1079
Bpu10I CC'TnA_GC
Cuts at: 2024 2024
Size: 6250
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2276 4375 5240 5538 2276
Size: 2099 865 298 2988
Fragments arranged by size:
2988 2099 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4913 882
Size: 617 3414 2219
Fragments arranged by size:
3414 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2929 4041 493
Size: 711 1725 1112 2702
Fragments arranged by size:
2702 1725 1112 711
BsaBI GATnn'nnATC
Cuts at: 6244 6244
Size: 6250
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2853 3309 3333 4057
Size: 53 83 186 936 1320 456 24 724
Cuts at: 4057 4439 5074 275
Size: 382 635 1451
Fragments arranged by size:
1451 1320 936 724 635 456 382 186
83 53 24
BsaWI w'CCGG_w
Cuts at: 4683 5081 5355 5502 5913 4683
Size: 398 274 147 411 5020
Fragments arranged by size:
5020 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2549 2552 2579 2582 1178
Size: 30 165 30 1146 3 27 3 4846
Fragments arranged by size:
4846 1146 165 30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2297 2557 3266 1186
Size: 1111 260 709 4170
Fragments arranged by size:
4170 1111 709 260
BseYI C'CCAG_C
Cuts at: 5453 5949 5453
Size: 496 5754
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2648 2918 3206 3395 1163
Size: 573 912 270 288 189 4018
Fragments arranged by size:
4018 912 573 288 270 189
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2439 3700 4461 5489 1540
Size: 275 186 438 1261 761 1028 2301
Fragments arranged by size:
2301 1261 1028 761 438 275 186
BsiHKAI G_wGCw'C
Cuts at: 2543 3378 4311 4396 5467 2543
Size: 835 933 85 1071 3326
Fragments arranged by size:
3326 1071 933 835 85
BsmI GAATG_Cn'
Cuts at: 2386 3761 3854 2386
Size: 1375 93 4782
Fragments arranged by size:
4782 1375 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2262 2543 2724 2991 3063 3378 3521
Size: 399 472 281 181 267 72 315 143
Cuts at: 3521 3569 4311 4396 5467 1391
Size: 48 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 472 399 315 281 267
181 143 85 72 48
BspEI T'CCGG_A
Cuts at: 5913 5913
Size: 6250
BspHI T'CATG_A
Cuts at: 4139 5869 4139
Size: 1730 4520
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2005 2541 3605 844
Size: 1161 536 1064 3489
Fragments arranged by size:
3489 1161 1064 536
BsrBI CCG'CTC
Cuts at: 1394 1823 4137 1394
Size: 429 2314 3507
Fragments arranged by size:
3507 2314 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4745 4919 65
Size: 1183 3497 174 1396
Fragments arranged by size:
3497 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2547 3134 4894 5081 1700
Size: 110 737 587 1760 187 2869
Fragments arranged by size:
2869 1760 737 587 187 110
BsrGI T'GTAC_A
Cuts at: 96 3204 96
Size: 3108 3142
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6250
BssSI C'ACGA_G
Cuts at: 2468 4310 5322 2468
Size: 1842 1012 3396
Fragments arranged by size:
3396 1842 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3361 794
Size: 2567 3683
BstBI TT'CG_AA
Cuts at: 5054 5054
Size: 6250
BstEII G'GTnAC_C
Cuts at: 1184 2126 1184
Size: 942 5308
BstXI CCAn_nnnn'nTGG
Cuts at: 2911 2911
Size: 6250
BstYI r'GATC_y
Cuts at: 1972 2046 2145 2589 2661 3580 3947 4332 4349
Size: 74 99 444 72 919 367 385 17
Cuts at: 4349 5801 5887 5899 6245 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 919 444 385 367 346 99
86 74 72 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2820 2982 2992 3471 513
Size: 552 939 816 162 10 479 3292
Fragments arranged by size:
3292 939 816 552 479 162 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2088 2677 487
Size: 683 56 862 589 4060
Fragments arranged by size:
4060 862 683 589 56
BtsI GCAGTG_nn'
Cuts at: 3161 3404 3766 4560 4580 3161
Size: 243 362 794 20 4831
Fragments arranged by size:
4831 794 362 243 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6250
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6215
DraI TTT'AAA
Cuts at: 3554 3554
Size: 6250
DraIII CAC_nnn'GTG
Cuts at: 3361 3361
Size: 6250
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5257 809
Size: 507 3941 1802
Fragments arranged by size:
3941 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 2332 2817
Size: 54 1425 50 275 18 303 199 485
Cuts at: 2817 3132 3697 4586 6215 8
Size: 315 565 889 1629 43
Fragments arranged by size:
1629 1425 889 565 485 315 303 275
199 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3697 1537
Size: 275 1885 4090
Fragments arranged by size:
4090 1885 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2052 2420 3488 4180 1353
Size: 699 368 1068 692 3423
Fragments arranged by size:
3423 1068 699 692 368
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4837 5211 5357 387
Size: 1064 138 3248 374 146 1280
Fragments arranged by size:
3248 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2541 3376 2541
Size: 835 5415
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2318 3446 4313 4910 5697 5965 1239
Size: 393 686 1128 867 597 787 268 1524
Fragments arranged by size:
1524 1128 867 787 686 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2524 2615 2524
Size: 91 6159
EcoO109I rG'GnC_Cy
Cuts at: 2530 3085 3467 2530
Size: 555 382 5313
Fragments arranged by size:
5313 555 382
EcoRI G'AATT_C
Cuts at: 3559 3937 3559
Size: 378 5872
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2740 2740
Size: 6250
FspI TGC'GCA
Cuts at: 3365 4756 3365
Size: 1391 4859
HaeII r_GCGC'y
Cuts at: 1678 2365 3322 5397 1678
Size: 687 957 2075 2531
Fragments arranged by size:
2531 2075 957 687
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2336 2368 2537 2569 2794
Size: 32 135 32 764 32 169 32 225
Cuts at: 2794 2826 4891 4923 4965 4997 1373
Size: 32 2065 32 42 32 2626
Fragments arranged by size:
2626 2065 764 225 169 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3594 3840 669
Size: 988 281 1656 246 3079
Fragments arranged by size:
3079 1656 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6250
HpaI GTT'AAC
Cuts at: 3840 3840
Size: 6250
KpnI G_GTAC'C
Cuts at: 2971 3575 2971
Size: 604 5646
MfeI C'AATT_G
Cuts at: 3849 3849
Size: 6250
MluI A'CGCG_T
Cuts at: 6126 6126
Size: 6250
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2070 2580 3237 5364 5548 1305
Size: 765 510 657 2127 184 2007
Fragments arranged by size:
2127 2007 765 657 510 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2135 2334 2819 10
Size: 54 1425 343 303 199 485 3441
Fragments arranged by size:
3441 1425 485 343 303 199 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2111 2379 2507 3212 4208 4567 4726
Size: 648 945 268 128 705 996 359 159
Cuts at: 4726 518
Size: 2042
Fragments arranged by size:
2042 996 945 705 648 359 268 159
128
MspA1I CmG'CkG
Cuts at: 1606 2994 3666 4343 5491 5736 1606
Size: 1388 672 677 1148 245 2120
Fragments arranged by size:
2120 1388 1148 677 672 245
NaeI GCC'GGC
Cuts at: 3136 3136
Size: 6250
NcoI C'CATG_G
Cuts at: 513 1065 2004 2820 513
Size: 552 939 816 3943
Fragments arranged by size:
3943 939 816 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6250
NgoMIV G'CCGG_C
Cuts at: 3134 3134
Size: 6250
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6250
NotI GC'GGCC_GC
Cuts at: 3697 3697
Size: 6250
NsiI A_TGCA'T
Cuts at: 2888 2888
Size: 6250
NspI r_CATG'y
Cuts at: 1132 1229 3238 3608 5153 1132
Size: 97 2009 370 1545 2229
Fragments arranged by size:
2229 2009 1545 370 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6250
PciI A'CATG_T
Cuts at: 5149 5149
Size: 6250
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2160 2163 2611 2929 3130 1108
Size: 1052 3 448 318 201 4228
Fragments arranged by size:
4228 1052 448 318 201 3
PfoI T'CCnGG_A
Cuts at: 2172 2772 3327 2172
Size: 600 555 5095
Fragments arranged by size:
5095 600 555
PmeI GTTT'AAAC
Cuts at: 3554 3554
Size: 6250
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4319 4351 5832 5864 4319
Size: 32 1481 32 4705
Fragments arranged by size:
4705 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2530 2530
Size: 6250
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6250
PsiI TTA'TAA
Cuts at: 3820 3820
Size: 6250
PstI C_TGCA'G
Cuts at: 830 2538 2802 3120 3602 830
Size: 1708 264 318 482 3478
Fragments arranged by size:
3478 1708 482 318 264
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6250
SacI G_AGCT'C
Cuts at: 2543 3378 2543
Size: 835 5415
SacII CC_GC'GG
Cuts at: 2995 2995
Size: 6250
SalI G'TCGA_C
Cuts at: 1936 3592 1936
Size: 1656 4594
SapI GCTCTTCn'nnn_
Cuts at: 2052 2052
Size: 6250
SbfI CC_TGCA'GG
Cuts at: 3602 3602
Size: 6250
ScaI AGT'ACT
Cuts at: 4498 6210 4498
Size: 1712 4538
SfcI C'TryA_G
Cuts at: 826 1045 2534 2798 3116 3598 4733 5414 5605
Size: 219 1489 264 318 482 1135 681 191
Cuts at: 5605 826
Size: 1471
Fragments arranged by size:
1489 1471 1135 681 482 318 264 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6250
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6250
SmaI CCC'GGG
Cuts at: 1872 3577 1872
Size: 1705 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2291 4354 5255 5517 820
Size: 197 926 348 2063 901 262 1553
Fragments arranged by size:
2063 1553 926 901 348 262 197
SnaBI TAC'GTA
Cuts at: 493 4041 493
Size: 3548 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6250
SphI G_CATG'C
Cuts at: 1132 3608 1132
Size: 2476 3774
SspI AAT'ATT
Cuts at: 5 52 4174 5
Size: 47 4122 2081
Fragments arranged by size:
4122 2081 47
StuI AGG'CCT
Cuts at: 1517 3386 1517
Size: 1869 4381
StyI C'CwwG_G
Cuts at: 513 1065 2004 2820 3689 513
Size: 552 939 816 869 3074
Fragments arranged by size:
3074 939 869 816 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2105 3520 4277 4294 4447 4632 5077
Size: 1255 525 1415 757 17 153 185 445
Cuts at: 5077 325
Size: 1498
Fragments arranged by size:
1498 1415 1255 757 525 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3204 4496 6208
Size: 274 80 33 51 1443 1227 1292 1712
Cuts at: 6208 96
Size: 138
Fragments arranged by size:
1712 1443 1292 1227 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2552 2584 6210 6242 2552
Size: 32 3626 32 2560
Fragments arranged by size:
3626 2560 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6250
XbaI T'CTAG_A
Cuts at: 3586 3586
Size: 6250
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2968 2968
Size: 6250
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6250
XmaI C'CCGG_G
Cuts at: 1870 3575 1870
Size: 1705 4545
XmnI GAAnn'nnTTC
Cuts at: 2519 4379 2519
Size: 1860 4390
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2854 3334 4058 276
Size: 53 83 186 936 1320 480 724 2468
Fragments arranged by size:
2468 1320 936 724 480 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SacII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI
KasI NarI NruI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SanDI SexAI SfiI SfoI SrfI SwaI