Restriction map for
pFN21AE4492
(Circular) (Six-base) MAPSORT of: pFN21AE4492.seq Check: 3669 from: 1 to: 6241
pFN21AE4492 6241 bp
With 182 enzymes: SgfI *
March 28, 2012 13:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2106 2106
Size: 6241
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4051 278
Size: 53 83 186 936 2515 2468
Fragments arranged by size:
2515 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2483 3584 1937
Size: 546 1101 4594
Fragments arranged by size:
4594 1101 546
Acc65I G'GTAC_C
Cuts at: 3562 3562
Size: 6241
AclI AA'CG_TT
Cuts at: 1563 4368 4741 1563
Size: 2805 373 3063
Fragments arranged by size:
3063 2805 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2474 2949 3533 4304 5688 5956 1632
Size: 842 475 584 771 1384 268 1917
Fragments arranged by size:
1917 1384 842 771 584 475 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6044
AflIII A'CryG_T
Cuts at: 1203 2486 5140 6117 1203
Size: 1283 2654 977 1327
Fragments arranged by size:
2654 1327 1283 977
AgeI A'CCGG_T
Cuts at: 5072 5072
Size: 6241
AhdI GACnn_n'nnGTC
Cuts at: 4970 4970
Size: 6241
AleI CACnn'nnGTG
Cuts at: 1166 3185 1166
Size: 2019 4222
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3252 3396 1184
Size: 2068 144 4029
Fragments arranged by size:
4029 2068 144
AlwNI CAG_nnn'CTG
Cuts at: 3363 3984 5556 3363
Size: 621 1572 4048
Fragments arranged by size:
4048 1572 621
ApaI G_GGCC'C
Cuts at: 2227 2400 3357 3527 2227
Size: 173 957 170 4941
Fragments arranged by size:
4941 957 173 170
ApaLI G'TGCA_C
Cuts at: 2587 4298 5454 2587
Size: 1711 1156 3374
Fragments arranged by size:
3374 1711 1156
ApoI r'AATT_y
Cuts at: 1493 2138 3550 3776 3928 6037 1493
Size: 645 1412 226 152 2109 1697
Fragments arranged by size:
2109 1697 1412 645 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4795 160
Size: 872 3763 1606
Fragments arranged by size:
3763 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6241
AvaI C'yCGr_G
Cuts at: 1870 1943 2180 2520 2883 3566 1870
Size: 73 237 340 363 683 4545
Fragments arranged by size:
4545 683 363 340 237 73
AvrII C'CTAG_G
Cuts at: 3052 3052
Size: 6241
BamHI G'GATC_C
Cuts at: 3571 3938 3571
Size: 367 5874
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2850 2879 3562 5017
Size: 325 217 627 221 842 29 683 1455
Cuts at: 5017 618
Size: 1842
Fragments arranged by size:
1842 1455 842 683 627 325 221 217
29
BanII G_rGCy'C
Cuts at: 1391 2227 2400 3357 3527 3560 1391
Size: 836 173 957 170 33 4072
Fragments arranged by size:
4072 957 836 173 170 33
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5270
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3005 3039 4432 4466 1903
Size: 34 1068 34 1393 34 3678
Fragments arranged by size:
3678 1393 1068 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4133 5343 4133
Size: 1210 5031
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6241
BfrBI ATG'CAT
Cuts at: 3229 3311 3229
Size: 82 6159
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4852 136
Size: 107 122 71 631 605 3180 1525
Fragments arranged by size:
3180 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6236 6236
Size: 6241
BlpI GC'TnA_GC
Cuts at: 3167 3658 3167
Size: 491 5750
Bme1580I G_kGCm'C
Cuts at: 1790 2013 2227 2400 2591 2884 3150 3357 3527
Size: 223 214 173 191 293 266 207 170
Cuts at: 3527 4302 5458 1790
Size: 775 1156 2573
Fragments arranged by size:
2573 1156 775 293 266 223 214 207
191 173 170
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6241
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3530 3972 4930
Size: 416 53 83 399 24 2113 442 958
Cuts at: 4930 442
Size: 1753
Fragments arranged by size:
2113 1753 958 442 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2345 4901 1239
Size: 1106 2556 2579
Fragments arranged by size:
2579 2556 1106
Bpu10I CC'TnA_GC
Cuts at: 2027 2124 3012 2027
Size: 97 888 5256
Fragments arranged by size:
5256 888 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2069 2411 4366 5231 5529 2069
Size: 342 1955 865 298 2781
Fragments arranged by size:
2781 1955 865 342 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3204 4904 882
Size: 617 1705 1700 2219
Fragments arranged by size:
2219 1705 1700 617
BsaAI yAC'GTr
Cuts at: 493 1204 2489 4032 493
Size: 711 1285 1543 2702
Fragments arranged by size:
2702 1543 1285 711
BsaBI GATnn'nnATC
Cuts at: 6235 6235
Size: 6241
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4048 4430 5065 275
Size: 53 83 186 936 2515 382 635 1451
Fragments arranged by size:
2515 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2844 4674 5072 5346 5493 5904 2844
Size: 1830 398 274 147 411 3181
Fragments arranged by size:
3181 1830 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2516 2546 1178
Size: 30 165 30 1113 30 4873
Fragments arranged by size:
4873 1113 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2026 2616 3050 1186
Size: 840 590 434 4377
Fragments arranged by size:
4377 840 590 434
BseYI C'CCAG_C
Cuts at: 2012 3350 3471 5444 5940 2012
Size: 1338 121 1973 496 2313
Fragments arranged by size:
2313 1973 1338 496 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5668
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3691 4452 5480 1540
Size: 275 186 1690 761 1028 2301
Fragments arranged by size:
2301 1690 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2591 3018 4302 4387 5458 2591
Size: 427 1284 85 1071 3374
Fragments arranged by size:
3374 1284 1071 427 85
BsmI GAATG_Cn'
Cuts at: 3313 3752 3845 3313
Size: 439 93 5709
Fragments arranged by size:
5709 439 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2186 2186
Size: 6241
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2227 2400 2591 2884 3018 3150
Size: 399 223 214 173 191 293 134 132
Cuts at: 3150 3357 3527 3560 4302 4387 5458 1391
Size: 207 170 33 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 293 223 214 207
191 173 170 134 132 85 33
BspEI T'CCGG_A
Cuts at: 5904 5904
Size: 6241
BspHI T'CATG_A
Cuts at: 2580 4130 5860 2580
Size: 1550 1730 2961
Fragments arranged by size:
2961 1730 1550
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2106 3214 3596 844
Size: 1262 1108 382 3489
Fragments arranged by size:
3489 1262 1108 382
BsrBI CCG'CTC
Cuts at: 1394 1823 2797 4128 1394
Size: 429 974 1331 3507
Fragments arranged by size:
3507 1331 974 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4736 4910 65
Size: 1183 3488 174 1396
Fragments arranged by size:
3488 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2609 3195 4885 5072 1700
Size: 110 799 586 1690 187 2869
Fragments arranged by size:
2869 1690 799 586 187 110
BsrGI T'GTAC_A
Cuts at: 96 2313 96
Size: 2217 4024
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6241
BssSI C'ACGA_G
Cuts at: 4301 5313 4301
Size: 1012 5229
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6241
BstBI TT'CG_AA
Cuts at: 5045 5045
Size: 6241
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6241
BstXI CCAn_nnnn'nTGG
Cuts at: 2369 2369
Size: 6241
BstYI r'GATC_y
Cuts at: 1972 2184 2835 3048 3571 3938 4323 4340 5792
Size: 212 651 213 523 367 385 17 1452
Cuts at: 5792 5878 5890 6236 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 651 523 385 367 346 213
212 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2331 3273 2331
Size: 942 5299
BtgI C'CryG_G
Cuts at: 513 1065 2004 2320 513
Size: 552 939 316 4434
Fragments arranged by size:
4434 939 552 316
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2565 487
Size: 683 56 1339 4163
Fragments arranged by size:
4163 1339 683 56
BtsI GCAGTG_nn'
Cuts at: 3757 4551 4571 3757
Size: 794 20 5427
Fragments arranged by size:
5427 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6241
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6206
DraI TTT'AAA
Cuts at: 3545 3545
Size: 6241
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3275 5248 809
Size: 507 1959 1973 1802
Fragments arranged by size:
1973 1959 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2466 2734 3193
Size: 54 1425 50 275 18 636 268 459
Cuts at: 3193 3688 4577 6206 8
Size: 495 889 1629 43
Fragments arranged by size:
1629 1425 889 636 495 459 275 268
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3688 1537
Size: 275 1876 4090
Fragments arranged by size:
4090 1876 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2505 2593 4171 1353
Size: 1152 88 1578 3423
Fragments arranged by size:
3423 1578 1152 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3119 4828 5202 5348 387
Size: 1064 138 1530 1709 374 146 1280
Fragments arranged by size:
1709 1530 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2345 2474 2949 3533 4304 4901 5688
Size: 393 713 129 475 584 771 597 787
Cuts at: 5688 5956 1239
Size: 268 1524
Fragments arranged by size:
1524 787 771 713 597 584 475 393
268 129
EcoO109I rG'GnC_Cy
Cuts at: 2147 2326 2335 2396 3524 2147
Size: 179 9 61 1128 4864
Fragments arranged by size:
4864 1128 179 61 9
EcoRI G'AATT_C
Cuts at: 3550 3928 3550
Size: 378 5863
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2608 3142 3514 2608
Size: 534 372 5335
Fragments arranged by size:
5335 534 372
FspI TGC'GCA
Cuts at: 4747 4747
Size: 6241
HaeII r_GCGC'y
Cuts at: 1678 2570 2683 5388 1678
Size: 892 113 2705 2531
Fragments arranged by size:
2705 2531 892 113
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2163 2195 2516 2548 3024
Size: 32 135 32 591 32 321 32 476
Cuts at: 3024 3056 3182 3214 3296 3328 4882 4914 4956
Size: 32 126 32 82 32 1554 32 42
Cuts at: 4956 4988 1373
Size: 32 2626
Fragments arranged by size:
2626 1554 591 476 321 135 126 82
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3585 3831 669
Size: 988 281 1647 246 3079
Fragments arranged by size:
3079 1647 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6241
HpaI GTT'AAC
Cuts at: 3831 3831
Size: 6241
KpnI G_GTAC'C
Cuts at: 3566 3566
Size: 6241
MfeI C'AATT_G
Cuts at: 3840 3840
Size: 6241
MluI A'CGCG_T
Cuts at: 6117 6117
Size: 6241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5355 5539 1305
Size: 4050 184 2007
Fragments arranged by size:
4050 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4419
Fragments arranged by size:
4419 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2445 2579 2942 3185 3234 3410 3464
Size: 648 1279 134 363 243 49 176 54
Cuts at: 3464 4199 4558 4717 518
Size: 735 359 159 2042
Fragments arranged by size:
2042 1279 735 648 363 359 243 176
159 134 54 49
MspA1I CmG'CkG
Cuts at: 1606 3657 4334 5482 5727 1606
Size: 2051 677 1148 245 2120
Fragments arranged by size:
2120 2051 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4750
Fragments arranged by size:
4750 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6241
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6241
NotI GC'GGCC_GC
Cuts at: 3688 3688
Size: 6241
NsiI A_TGCA'T
Cuts at: 3231 3313 3231
Size: 82 6159
NspI r_CATG'y
Cuts at: 1132 1229 2177 3599 5144 1132
Size: 97 948 1422 1545 2229
Fragments arranged by size:
2229 1545 1422 948 97
PasI CC'CwG_GG
Cuts at: 1348 2144 3527 1348
Size: 796 1383 4062
Fragments arranged by size:
4062 1383 796
PciI A'CATG_T
Cuts at: 5140 5140
Size: 6241
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2160 1108
Size: 1052 5189
PfoI T'CCnGG_A
Cuts at: 2187 2862 2187
Size: 675 5566
PmeI GTTT'AAAC
Cuts at: 3545 3545
Size: 6241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4310 4342 5823 5855 4310
Size: 32 1481 32 4696
Fragments arranged by size:
4696 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2326 2326
Size: 6241
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6241
PsiI TTA'TAA
Cuts at: 3811 3811
Size: 6241
PspOMI G'GGCC_C
Cuts at: 2223 2396 3353 3523 2223
Size: 173 957 170 4941
Fragments arranged by size:
4941 957 173 170
PstI C_TGCA'G
Cuts at: 830 2814 3211 3593 830
Size: 1984 397 382 3478
Fragments arranged by size:
3478 1984 397 382
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6241
SalI G'TCGA_C
Cuts at: 1936 3583 1936
Size: 1647 4594
SanDI GG'GwC_CC
Cuts at: 2326 2326
Size: 6241
SbfI CC_TGCA'GG
Cuts at: 2814 3211 3593 2814
Size: 397 382 5462
Fragments arranged by size:
5462 397 382
ScaI AGT'ACT
Cuts at: 4489 6201 4489
Size: 1712 4529
SexAI A'CCwGG_T
Cuts at: 2781 2781
Size: 6241
SfcI C'TryA_G
Cuts at: 826 1045 2810 3207 3589 4724 5405 5596 826
Size: 219 1765 397 382 1135 681 191 1471
Fragments arranged by size:
1765 1471 1135 681 397 382 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6241
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6241
SmaI CCC'GGG
Cuts at: 1872 2885 3568 1872
Size: 1013 683 4545
Fragments arranged by size:
4545 1013 683
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2084 2180 2390 4345 5246 5508
Size: 197 926 141 96 210 1955 901 262
Cuts at: 5508 820
Size: 1553
Fragments arranged by size:
1955 1553 926 901 262 210 197 141
96
SnaBI TAC'GTA
Cuts at: 493 4032 493
Size: 3539 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6241
SphI G_CATG'C
Cuts at: 1132 3599 1132
Size: 2467 3774
SspI AAT'ATT
Cuts at: 5 52 4165 5
Size: 47 4113 2081
Fragments arranged by size:
4113 2081 47
StuI AGG'CCT
Cuts at: 1517 2379 1517
Size: 862 5379
StyI C'CwwG_G
Cuts at: 513 1065 2004 2040 3052 3680 513
Size: 552 939 36 1012 628 3074
Fragments arranged by size:
3074 1012 939 628 552 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2171 2262 2867 4268 4285 4438 4623
Size: 1255 591 91 605 1401 17 153 185
Cuts at: 4623 5068 325
Size: 445 1498
Fragments arranged by size:
1498 1401 1255 605 591 445 185 153
91 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2313 4487 6199
Size: 274 80 33 51 1443 336 2174 1712
Cuts at: 6199 96
Size: 138
Fragments arranged by size:
2174 1712 1443 336 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6201 6233 6201
Size: 32 6209
Tth111I GACn'n_nGTC
Cuts at: 1316 2783 1316
Size: 1467 4774
XbaI T'CTAG_A
Cuts at: 3577 3577
Size: 6241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2165 2165
Size: 6241
XhoI C'TCGA_G
Cuts at: 1943 2180 1943
Size: 237 6004
XmaI C'CCGG_G
Cuts at: 1870 2883 3566 1870
Size: 1013 683 4545
Fragments arranged by size:
4545 1013 683
XmnI GAAnn'nnTTC
Cuts at: 4370 4370
Size: 6241
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4049 276
Size: 53 83 186 936 2515 2468
Fragments arranged by size:
2515 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SalI SanDI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BbvCI BplI BsiWI
BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI
PvuII RsrII SacI SacII SapI SfiI SfoI SrfI
SwaI