Restriction map for
pFN21AE4490
(Circular) (Six-base) MAPSORT of: pFN21AE4490.seq Check: 9173 from: 1 to: 6238
pFN21AE4490 6238 bp
With 182 enzymes: SgfI *
March 28, 2012 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2515 2854 2515
Size: 339 5899
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2215 4048 278
Size: 53 83 186 936 679 1833 2468
Fragments arranged by size:
2468 1833 936 679 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3581 1937
Size: 1644 4594
Acc65I G'GTAC_C
Cuts at: 3559 3559
Size: 6238
AclI AA'CG_TT
Cuts at: 1563 2741 4365 4738 1563
Size: 1178 1624 373 3063
Fragments arranged by size:
3063 1624 1178 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4301 5685 5953 1632
Size: 2669 1384 268 1917
Fragments arranged by size:
2669 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6041
AflIII A'CryG_T
Cuts at: 1203 5137 6114 1203
Size: 3934 977 1327
Fragments arranged by size:
3934 1327 977
AgeI A'CCGG_T
Cuts at: 5069 5069
Size: 6238
AhdI GACnn_n'nnGTC
Cuts at: 4967 4967
Size: 6238
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6238
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2547 1184
Size: 1363 4875
AlwNI CAG_nnn'CTG
Cuts at: 2572 2656 3981 5553 2572
Size: 84 1325 1572 3257
Fragments arranged by size:
3257 1572 1325 84
ApaLI G'TGCA_C
Cuts at: 4295 5451 4295
Size: 1156 5082
ApoI r'AATT_y
Cuts at: 1493 3547 3773 3925 6034 1493
Size: 2054 226 152 2109 1697
Fragments arranged by size:
2109 2054 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2294 4792 160
Size: 872 1262 2498 1606
Fragments arranged by size:
2498 1606 1262 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6238
AvaI C'yCGr_G
Cuts at: 1870 1943 2582 3563 1870
Size: 73 639 981 4545
Fragments arranged by size:
4545 981 639 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3145 3178 3145
Size: 33 6205
BamHI G'GATC_C
Cuts at: 2958 3568 3935 2958
Size: 610 367 5261
Fragments arranged by size:
5261 610 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2105 3281 3559 5014 618
Size: 325 217 627 318 1176 278 1455 1842
Fragments arranged by size:
1842 1455 1176 627 325 318 278 217
BanII G_rGCy'C
Cuts at: 1391 2848 3087 3318 3557 1391
Size: 1457 239 231 239 4072
Fragments arranged by size:
4072 1457 239 239 231
BbeI G_GCGC'C
Cuts at: 2109 2109
Size: 6238
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3016 928
Size: 971 1117 4150
Fragments arranged by size:
4150 1117 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4429 4463 1903
Size: 34 2492 34 3678
Fragments arranged by size:
3678 2492 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4130 5340 4130
Size: 1210 5028
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6238
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4849 136
Size: 107 122 71 631 605 3177 1525
Fragments arranged by size:
3177 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2490 6233 2490
Size: 3743 2495
BlpI GC'TnA_GC
Cuts at: 2657 3655 2657
Size: 998 5240
Bme1580I G_kGCm'C
Cuts at: 1790 4299 5455 1790
Size: 2509 1156 2573
Fragments arranged by size:
2573 2509 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6238
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2571 3969 4927
Size: 416 53 83 399 24 1154 1398 958
Cuts at: 4927 442
Size: 1753
Fragments arranged by size:
1753 1398 1154 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2378 2852 4898 1239
Size: 1139 474 2046 2579
Fragments arranged by size:
2579 2046 1139 474
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3010 4363 5228 5526 3010
Size: 1353 865 298 3722
Fragments arranged by size:
3722 1353 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3052 3413 4901 882
Size: 617 1553 361 1488 2219
Fragments arranged by size:
2219 1553 1488 617 361
BsaAI yAC'GTr
Cuts at: 493 1204 4029 493
Size: 711 2825 2702
Fragments arranged by size:
2825 2702 711
BsaBI GATnn'nnATC
Cuts at: 6232 6232
Size: 6238
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2106 2212 2454 4045
Size: 53 83 186 936 573 106 242 1591
Cuts at: 4045 4427 5062 275
Size: 382 635 1451
Fragments arranged by size:
1591 1451 936 635 573 382 242 186
106 83 53
BsaWI w'CCGG_w
Cuts at: 4671 5069 5343 5490 5901 4671
Size: 398 274 147 411 5008
Fragments arranged by size:
5008 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2616 2646 3281 3311 1178
Size: 30 165 30 1213 30 635 30 4105
Fragments arranged by size:
4105 1213 635 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2043 2075 2107 2792 2821 3056 3308 1186
Size: 857 32 32 685 29 235 252 4116
Fragments arranged by size:
4116 857 685 252 235 32 32 29
BseYI C'CCAG_C
Cuts at: 2590 2954 5441 5937 2590
Size: 364 2487 496 2891
Fragments arranged by size:
2891 2487 496 364
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2822 1163
Size: 573 1086 4579
Fragments arranged by size:
4579 1086 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3260 3688 4449 5477 1540
Size: 275 186 1259 428 761 1028 2301
Fragments arranged by size:
2301 1259 1028 761 428 275 186
BsiHKAI G_wGCw'C
Cuts at: 2472 2848 3048 4299 4384 5455 2472
Size: 376 200 1251 85 1071 3255
Fragments arranged by size:
3255 1251 1071 376 200 85
BsmI GAATG_Cn'
Cuts at: 2158 3171 3749 3842 2158
Size: 1013 578 93 4554
Fragments arranged by size:
4554 1013 578 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2472 2848 3048 3087 3318 3557 4299
Size: 399 682 376 200 39 231 239 742
Cuts at: 4299 4384 5455 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 682 399 376 239 231
200 85 39
BspEI T'CCGG_A
Cuts at: 5901 5901
Size: 6238
BspHI T'CATG_A
Cuts at: 4127 5857 4127
Size: 1730 4508
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2515 2700 2854 3593 844
Size: 1671 185 154 739 3489
Fragments arranged by size:
3489 1671 739 185 154
BsrBI CCG'CTC
Cuts at: 1394 1823 4125 1394
Size: 429 2302 3507
Fragments arranged by size:
3507 2302 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2372 3273 4733 4907 65
Size: 1183 1124 901 1460 174 1396
Fragments arranged by size:
1460 1396 1183 1124 901 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3259 4882 5069 1700
Size: 110 1449 1623 187 2869
Fragments arranged by size:
2869 1623 1449 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6238
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6238
BssSI C'ACGA_G
Cuts at: 2087 4298 5310 2087
Size: 2211 1012 3015
Fragments arranged by size:
3015 2211 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2338 2366 794
Size: 1544 28 4666
Fragments arranged by size:
4666 1544 28
BstBI TT'CG_AA
Cuts at: 5042 5042
Size: 6238
BstEII G'GTnAC_C
Cuts at: 1184 2869 1184
Size: 1685 4553
BstXI CCAn_nnnn'nTGG
Cuts at: 2906 2906
Size: 6238
BstYI r'GATC_y
Cuts at: 1972 2490 2505 2958 2973 3568 3935 4320 4337
Size: 518 15 453 15 595 367 385 17
Cuts at: 4337 5789 5875 5887 6233 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 595 518 453 385 367 346
86 17 15 15 12
BtgI C'CryG_G
Cuts at: 513 1065 2509 2886 513
Size: 552 1444 377 3865
Fragments arranged by size:
3865 1444 552 377
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3169 487
Size: 683 56 1943 3556
Fragments arranged by size:
3556 1943 683 56
BtsI GCAGTG_nn'
Cuts at: 3205 3754 4548 4568 3205
Size: 549 794 20 4875
Fragments arranged by size:
4875 794 549 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6238
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6203
DraI TTT'AAA
Cuts at: 3542 3542
Size: 6238
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3506 5245 809
Size: 507 2190 1739 1802
Fragments arranged by size:
2190 1802 1739 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2343 2609 3177
Size: 54 1425 50 275 18 513 266 568
Cuts at: 3177 3257 3685 4574 6203 8
Size: 80 428 889 1629 43
Fragments arranged by size:
1629 1425 889 568 513 428 275 266
80 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3257 3685 1537
Size: 275 1445 428 4090
Fragments arranged by size:
4090 1445 428 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3101 3326 3483 4168 1353
Size: 1748 225 157 685 3423
Fragments arranged by size:
3423 1748 685 225 157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2804 4825 5199 5345 387
Size: 1064 138 1215 2021 374 146 1280
Fragments arranged by size:
2021 1280 1215 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2846 2846
Size: 6238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2378 2852 4301 4898 5685 5953 1239
Size: 393 746 474 1449 597 787 268 1524
Fragments arranged by size:
1524 1449 787 746 597 474 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2782 3105 3148 2782
Size: 323 43 5872
Fragments arranged by size:
5872 323 43
EcoRI G'AATT_C
Cuts at: 3547 3925 3547
Size: 378 5860
FspI TGC'GCA
Cuts at: 4744 4744
Size: 6238
HaeII r_GCGC'y
Cuts at: 1678 2109 5385 1678
Size: 431 3276 2531
Fragments arranged by size:
3276 2531 431
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2418 2450 3051 3083 4879
Size: 32 135 32 846 32 601 32 1796
Cuts at: 4879 4911 4953 4985 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1796 846 601 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3289 3582 3828 669
Size: 988 281 1351 293 246 3079
Fragments arranged by size:
3079 1351 988 293 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6238
HpaI GTT'AAC
Cuts at: 3828 3828
Size: 6238
KasI G'GCGC_C
Cuts at: 2105 2105
Size: 6238
KpnI G_GTAC'C
Cuts at: 3563 3563
Size: 6238
MfeI C'AATT_G
Cuts at: 3837 3837
Size: 6238
MluI A'CGCG_T
Cuts at: 6114 6114
Size: 6238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3324 5352 5536 1305
Size: 2019 2028 184 2007
Fragments arranged by size:
2028 2019 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2345 2611 3179 10
Size: 54 1425 343 513 266 568 3069
Fragments arranged by size:
3069 1425 568 513 343 266 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2552 4196 4555 4714 518
Size: 648 1386 1644 359 159 2042
Fragments arranged by size:
2042 1644 1386 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2653 2859 3256 3506 3654 4331 5479 5724
Size: 1047 206 397 250 148 677 1148 245
Cuts at: 5724 1606
Size: 2120
Fragments arranged by size:
2120 1148 1047 677 397 250 245 206
148
NaeI GCC'GGC
Cuts at: 3261 3261
Size: 6238
NarI GG'CG_CC
Cuts at: 2106 2106
Size: 6238
NcoI C'CATG_G
Cuts at: 513 1065 2886 513
Size: 552 1821 3865
Fragments arranged by size:
3865 1821 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6238
NgoMIV G'CCGG_C
Cuts at: 3259 3259
Size: 6238
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6238
NotI GC'GGCC_GC
Cuts at: 3685 3685
Size: 6238
NspI r_CATG'y
Cuts at: 1132 1229 3596 5141 1132
Size: 97 2367 1545 2229
Fragments arranged by size:
2367 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3144 1348
Size: 1796 4442
PciI A'CATG_T
Cuts at: 5137 5137
Size: 6238
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6238
PfoI T'CCnGG_A
Cuts at: 2673 2673
Size: 6238
PmeI GTTT'AAAC
Cuts at: 3542 3542
Size: 6238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4307 4339 5820 5852 4307
Size: 32 1481 32 4693
Fragments arranged by size:
4693 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2782 3105 3148 2782
Size: 323 43 5872
Fragments arranged by size:
5872 323 43
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6238
PsiI TTA'TAA
Cuts at: 3808 3808
Size: 6238
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3461 3493 3461
Size: 32 6206
PstI C_TGCA'G
Cuts at: 830 3132 3200 3590 830
Size: 2302 68 390 3478
Fragments arranged by size:
3478 2302 390 68
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6238
PvuII CAG'CTG
Cuts at: 2653 3506 2653
Size: 853 5385
SacI G_AGCT'C
Cuts at: 2848 2848
Size: 6238
SalI G'TCGA_C
Cuts at: 1936 3580 1936
Size: 1644 4594
SanDI GG'GwC_CC
Cuts at: 3105 3148 3105
Size: 43 6195
SbfI CC_TGCA'GG
Cuts at: 3132 3590 3132
Size: 458 5780
ScaI AGT'ACT
Cuts at: 4486 6198 4486
Size: 1712 4526
SfcI C'TryA_G
Cuts at: 826 1045 3128 3196 3586 4721 5402 5593 826
Size: 219 2083 68 390 1135 681 191 1471
Fragments arranged by size:
2083 1471 1135 681 390 219 191 68
SfoI GGC'GCC
Cuts at: 2107 2107
Size: 6238
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6238
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6238
SmaI CCC'GGG
Cuts at: 1872 3565 1872
Size: 1693 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2989 4342 5243 5505 820
Size: 197 926 1046 1353 901 262 1553
Fragments arranged by size:
1553 1353 1046 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4029 493
Size: 3536 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6238
SphI G_CATG'C
Cuts at: 1132 3596 1132
Size: 2464 3774
SspI AAT'ATT
Cuts at: 5 52 4162 5
Size: 47 4110 2081
Fragments arranged by size:
4110 2081 47
StuI AGG'CCT
Cuts at: 1517 2177 3535 1517
Size: 660 1358 4220
Fragments arranged by size:
4220 1358 660
StyI C'CwwG_G
Cuts at: 513 1065 2499 2886 3677 513
Size: 552 1434 387 791 3074
Fragments arranged by size:
3074 1434 791 552 387
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2040 2512 4265 4282 4435 4620 5065
Size: 1255 460 472 1753 17 153 185 445
Cuts at: 5065 325
Size: 1498
Fragments arranged by size:
1753 1498 1255 472 460 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2993 4484 6196
Size: 274 80 33 51 1443 1016 1491 1712
Cuts at: 6196 96
Size: 138
Fragments arranged by size:
1712 1491 1443 1016 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2616 2648 6198 6230 2616
Size: 32 3550 32 2624
Fragments arranged by size:
3550 2624 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2257 1316
Size: 941 5297
XbaI T'CTAG_A
Cuts at: 3574 3574
Size: 6238
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6238
XmaI C'CCGG_G
Cuts at: 1870 3563 1870
Size: 1693 4545
XmnI GAAnn'nnTTC
Cuts at: 2732 4367 2732
Size: 1635 4603
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2213 4046 276
Size: 53 83 186 936 679 1833 2468
Fragments arranged by size:
2468 1833 936 679 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PspOMI
PspXI RsrII SacII SapI SexAI SfiI SrfI SwaI
XcmI