Restriction map for
pFN21AE4489
(Circular) (Six-base) MAPSORT of: pFN21AE4489.seq Check: 7589 from: 1 to: 6238
pFN21AE4489 6238 bp
With 182 enzymes: SgfI *
March 28, 2012 13:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4048 278
Size: 53 83 186 936 2512 2468
Fragments arranged by size:
2512 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2664 2962 2987 3581 1937
Size: 727 298 25 594 4594
Fragments arranged by size:
4594 727 594 298 25
Acc65I G'GTAC_C
Cuts at: 2953 3559 2953
Size: 606 5632
AclI AA'CG_TT
Cuts at: 1563 4365 4738 1563
Size: 2802 373 3063
Fragments arranged by size:
3063 2802 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2295 2937 3500 4301 5685 5953 1632
Size: 663 642 563 801 1384 268 1917
Fragments arranged by size:
1917 1384 801 663 642 563 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6041
AflIII A'CryG_T
Cuts at: 1203 2878 2982 5137 6114 1203
Size: 1675 104 2155 977 1327
Fragments arranged by size:
2155 1675 1327 977 104
AgeI A'CCGG_T
Cuts at: 2066 2368 5069 2066
Size: 302 2701 3235
Fragments arranged by size:
3235 2701 302
AhdI GACnn_n'nnGTC
Cuts at: 4967 4967
Size: 6238
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6238
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6238
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2108 2140 2108
Size: 32 6206
AlwNI CAG_nnn'CTG
Cuts at: 2101 2156 3981 5553 2101
Size: 55 1825 1572 2786
Fragments arranged by size:
2786 1825 1572 55
ApaLI G'TGCA_C
Cuts at: 4295 5451 4295
Size: 1156 5082
ApoI r'AATT_y
Cuts at: 1493 2568 2680 2852 3362 3509 3547 3773 3925
Size: 1075 112 172 510 147 38 226 152
Cuts at: 3925 6034 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1075 510 226 172 152 147
112 38
AseI AT'TA_AT
Cuts at: 160 1032 4792 160
Size: 872 3760 1606
Fragments arranged by size:
3760 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6238
AvaI C'yCGr_G
Cuts at: 1870 1943 2048 2088 3519 3563 1870
Size: 73 105 40 1431 44 4545
Fragments arranged by size:
4545 1431 105 73 44 40
BamHI G'GATC_C
Cuts at: 3568 3935 3568
Size: 367 5871
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2953 3559 5014 618
Size: 325 217 627 1166 606 1455 1842
Fragments arranged by size:
1842 1455 1166 627 606 325 217
BanII G_rGCy'C
Cuts at: 1391 2089 3557 1391
Size: 698 1468 4072
Fragments arranged by size:
4072 1468 698
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2454 928
Size: 971 555 4712
Fragments arranged by size:
4712 971 555
BbvCI CC'TCA_GC
Cuts at: 3503 3503
Size: 6238
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4429 4463 1903
Size: 34 2492 34 3678
Fragments arranged by size:
3678 2492 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4130 5340 4130
Size: 1210 5028
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6238
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4849 136
Size: 107 122 71 631 605 3177 1525
Fragments arranged by size:
3177 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2619 3132 6233 2619
Size: 513 3101 2624
Fragments arranged by size:
3101 2624 513
BlpI GC'TnA_GC
Cuts at: 3655 3655
Size: 6238
Bme1580I G_kGCm'C
Cuts at: 1790 2064 4299 5455 1790
Size: 274 2235 1156 2573
Fragments arranged by size:
2573 2235 1156 274
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6238
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2358 3969 4927
Size: 416 53 83 399 24 941 1611 958
Cuts at: 4927 442
Size: 1753
Fragments arranged by size:
1753 1611 958 941 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6238
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3507 3507
Size: 6238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2178 4898 1239
Size: 939 2720 2579
Fragments arranged by size:
2720 2579 939
Bpu10I CC'TnA_GC
Cuts at: 3396 3503 3396
Size: 107 6131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4363 5228 5526 4363
Size: 865 298 5075
Fragments arranged by size:
5075 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4901 882
Size: 617 3402 2219
Fragments arranged by size:
3402 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4029 493
Size: 711 2825 2702
Fragments arranged by size:
2825 2702 711
BsaBI GATnn'nnATC
Cuts at: 6232 6232
Size: 6238
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4045 4427 5062 275
Size: 53 83 186 936 2512 382 635 1451
Fragments arranged by size:
2512 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2066 2233 2368 4671 5069 5343 5490 5901 2066
Size: 167 135 2303 398 274 147 411 2403
Fragments arranged by size:
2403 2303 411 398 274 167 147 135
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2108 2138 1178
Size: 30 165 30 705 30 5278
Fragments arranged by size:
5278 705 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2817 1186
Size: 1631 4607
BseYI C'CCAG_C
Cuts at: 2127 2588 3017 5441 5937 2127
Size: 461 429 2424 496 2428
Fragments arranged by size:
2428 2424 496 461 429
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3137 1163
Size: 573 1401 4264
Fragments arranged by size:
4264 1401 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3688 4449 5477 1540
Size: 275 186 1687 761 1028 2301
Fragments arranged by size:
2301 1687 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2089 3500 4299 4384 5455 2089
Size: 1411 799 85 1071 2872
Fragments arranged by size:
2872 1411 1071 799 85
BsmI GAATG_Cn'
Cuts at: 3749 3842 3749
Size: 93 6145
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2089 3500 3557 4299 4384 5455
Size: 399 274 25 1411 57 742 85 1071
Cuts at: 5455 1391
Size: 2174
Fragments arranged by size:
2174 1411 1071 742 399 274 85 57
25
BspEI T'CCGG_A
Cuts at: 5901 5901
Size: 6238
BspHI T'CATG_A
Cuts at: 4127 5857 4127
Size: 1730 4508
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3593 844
Size: 2749 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4125 1394
Size: 429 2302 3507
Fragments arranged by size:
3507 2302 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3390 4733 4907 65
Size: 1183 2142 1343 174 1396
Fragments arranged by size:
2142 1396 1343 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2066 2074 2368 4882 5069 1700
Size: 110 256 8 294 2514 187 2869
Fragments arranged by size:
2869 2514 294 256 187 110 8
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6238
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6238
BssSI C'ACGA_G
Cuts at: 4298 5310 4298
Size: 1012 5226
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6238
BstBI TT'CG_AA
Cuts at: 5042 5042
Size: 6238
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6238
BstYI r'GATC_y
Cuts at: 1972 2619 3132 3568 3935 4320 4337 5789 5875
Size: 647 513 436 367 385 17 1452 86
Cuts at: 5875 5887 6233 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 647 513 436 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2988 2988
Size: 6238
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4747
Fragments arranged by size:
4747 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2033 487
Size: 683 56 807 4692
Fragments arranged by size:
4692 807 683 56
BtsI GCAGTG_nn'
Cuts at: 3754 4548 4568 3754
Size: 794 20 5424
Fragments arranged by size:
5424 794 20
ClaI AT'CG_AT
Cuts at: 1554 2535 1554
Size: 981 5257
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6203
DraI TTT'AAA
Cuts at: 3542 3542
Size: 6238
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5245 809
Size: 507 3929 1802
Fragments arranged by size:
3929 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2442 2529 3685
Size: 54 1425 50 275 18 612 87 1156
Cuts at: 3685 4574 6203 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1156 889 612 275 87 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3685 1537
Size: 275 1873 4090
Fragments arranged by size:
4090 1873 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2943 3476 4168 1353
Size: 1590 533 692 3423
Fragments arranged by size:
3423 1590 692 533
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4825 5199 5345 387
Size: 1064 138 3236 374 146 1280
Fragments arranged by size:
3236 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2087 2087
Size: 6238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2178 2295 2937 3500 4301 4898 5685
Size: 393 546 117 642 563 801 597 787
Cuts at: 5685 5953 1239
Size: 268 1524
Fragments arranged by size:
1524 801 787 642 597 563 546 393
268 117
EcoO109I rG'GnC_Cy
Cuts at: 2429 3401 2429
Size: 972 5266
EcoRI G'AATT_C
Cuts at: 3547 3925 3547
Size: 378 5860
EcoRV GAT'ATC
Cuts at: 2461 3085 2461
Size: 624 5614
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2471 3350 2471
Size: 879 5359
FspI TGC'GCA
Cuts at: 4744 4744
Size: 6238
HaeII r_GCGC'y
Cuts at: 1678 2012 2102 5385 1678
Size: 334 90 3283 2531
Fragments arranged by size:
3283 2531 334 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2170 2202 2599 2631 4879
Size: 32 135 32 598 32 397 32 2248
Cuts at: 4879 4911 4953 4985 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2248 598 397 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2035 2963 3582 3828 669
Size: 988 281 97 928 619 246 3079
Fragments arranged by size:
3079 988 928 619 281 246 97
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6238
HpaI GTT'AAC
Cuts at: 3828 3828
Size: 6238
KpnI G_GTAC'C
Cuts at: 2957 3563 2957
Size: 606 5632
MfeI C'AATT_G
Cuts at: 2688 3837 2688
Size: 1149 5089
MluI A'CGCG_T
Cuts at: 6114 6114
Size: 6238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2093 3301 5352 5536 1305
Size: 788 1208 2051 184 2007
Fragments arranged by size:
2051 2007 1208 788 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4416
Fragments arranged by size:
4416 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3252 4196 4555 4714 518
Size: 648 2086 944 359 159 2042
Fragments arranged by size:
2086 2042 944 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2849 3151 3654 4331 5479 5724 1606
Size: 1243 302 503 677 1148 245 2120
Fragments arranged by size:
2120 1243 1148 677 503 302 245
NaeI GCC'GGC
Cuts at: 2076 2076
Size: 6238
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4747
Fragments arranged by size:
4747 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6238
NgoMIV G'CCGG_C
Cuts at: 2074 2074
Size: 6238
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6238
NotI GC'GGCC_GC
Cuts at: 3685 3685
Size: 6238
NspI r_CATG'y
Cuts at: 1132 1229 2882 2986 3539 3596 5141 1132
Size: 97 1653 104 553 57 1545 2229
Fragments arranged by size:
2229 1653 1545 553 104 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6238
PciI A'CATG_T
Cuts at: 2878 2982 5137 2878
Size: 104 2155 3979
Fragments arranged by size:
3979 2155 104
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6238
PmeI GTTT'AAAC
Cuts at: 3542 3542
Size: 6238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2108 2140 4307 4339 5820 5852 2108
Size: 32 2167 32 1481 32 2494
Fragments arranged by size:
2494 2167 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2429 3401 2429
Size: 972 5266
PshAI GACnn'nnGTC
Cuts at: 1613 2427 1613
Size: 814 5424
PsiI TTA'TAA
Cuts at: 3808 3808
Size: 6238
PstI C_TGCA'G
Cuts at: 830 3372 3590 830
Size: 2542 218 3478
Fragments arranged by size:
3478 2542 218
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6238
PvuII CAG'CTG
Cuts at: 2849 3151 2849
Size: 302 5936
SacI G_AGCT'C
Cuts at: 2089 2089
Size: 6238
SalI G'TCGA_C
Cuts at: 1936 2961 3580 1936
Size: 1025 619 4594
Fragments arranged by size:
4594 1025 619
SbfI CC_TGCA'GG
Cuts at: 3590 3590
Size: 6238
ScaI AGT'ACT
Cuts at: 4486 6198 4486
Size: 1712 4526
SexAI A'CCwGG_T
Cuts at: 2956 2956
Size: 6238
SfcI C'TryA_G
Cuts at: 826 1045 2668 3368 3586 4721 5402 5593 826
Size: 219 1623 700 218 1135 681 191 1471
Fragments arranged by size:
1623 1471 1135 700 681 219 218 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6238
SgrAI Cr'CCGG_yG
Cuts at: 1700 2066 2368 1700
Size: 366 302 5570
Fragments arranged by size:
5570 366 302
SmaI CCC'GGG
Cuts at: 1872 3565 1872
Size: 1693 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3519 4342 5243 5505 820
Size: 197 926 1576 823 901 262 1553
Fragments arranged by size:
1576 1553 926 901 823 262 197
SnaBI TAC'GTA
Cuts at: 493 4029 493
Size: 3536 2702
SpeI A'CTAG_T
Cuts at: 152 2173 152
Size: 2021 4217
SphI G_CATG'C
Cuts at: 1132 3596 1132
Size: 2464 3774
SspI AAT'ATT
Cuts at: 5 52 4162 5
Size: 47 4110 2081
Fragments arranged by size:
4110 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6238
StyI C'CwwG_G
Cuts at: 513 1065 2004 2280 3677 513
Size: 552 939 276 1397 3074
Fragments arranged by size:
3074 1397 939 552 276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2696 4265 4282 4435 4620 5065 325
Size: 1255 1116 1569 17 153 185 445 1498
Fragments arranged by size:
1569 1498 1255 1116 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2437 4484 6196
Size: 274 80 33 51 1443 460 2047 1712
Cuts at: 6196 96
Size: 138
Fragments arranged by size:
2047 1712 1443 460 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3282 3314 6198 6230 3282
Size: 32 2884 32 3290
Fragments arranged by size:
3290 2884 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2771 1316
Size: 1455 4783
XbaI T'CTAG_A
Cuts at: 3574 3574
Size: 6238
XhoI C'TCGA_G
Cuts at: 1943 3519 1943
Size: 1576 4662
XmaI C'CCGG_G
Cuts at: 1870 3563 1870
Size: 1693 4545
XmnI GAAnn'nnTTC
Cuts at: 2511 2922 4367 2511
Size: 411 1445 4382
Fragments arranged by size:
4382 1445 411
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4046 276
Size: 53 83 186 936 2512 2468
Fragments arranged by size:
2512 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI Bsu36I DraIII EcoNI FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI
SrfI SwaI XcmI