Restriction map for
pFN21AE4486
(Circular) (Six-base) MAPSORT of: pFN21AE4486.seq Check: 3883 from: 1 to: 6211
pFN21AE4486 6211 bp
With 182 enzymes: SgfI *
March 28, 2012 13:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4021 278
Size: 53 83 186 936 2485 2468
Fragments arranged by size:
2485 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3554 1937
Size: 1617 4594
Acc65I G'GTAC_C
Cuts at: 3532 3532
Size: 6211
AclI AA'CG_TT
Cuts at: 1563 4338 4711 1563
Size: 2775 373 3063
Fragments arranged by size:
3063 2775 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2804 3365 4274 5658 5926 1632
Size: 1172 561 909 1384 268 1917
Fragments arranged by size:
1917 1384 1172 909 561 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6014
AflIII A'CryG_T
Cuts at: 1203 5110 6087 1203
Size: 3907 977 1327
Fragments arranged by size:
3907 1327 977
AgeI A'CCGG_T
Cuts at: 5042 5042
Size: 6211
AhdI GACnn_n'nnGTC
Cuts at: 4940 4940
Size: 6211
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6211
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6211
AlwNI CAG_nnn'CTG
Cuts at: 2995 3236 3324 3954 5526 2995
Size: 241 88 630 1572 3680
Fragments arranged by size:
3680 1572 630 241 88
ApaI G_GGCC'C
Cuts at: 2127 2127
Size: 6211
ApaLI G'TGCA_C
Cuts at: 4268 5424 4268
Size: 1156 5055
ApoI r'AATT_y
Cuts at: 1493 2020 2115 2180 2639 2743 2842 3520 3746
Size: 527 95 65 459 104 99 678 226
Cuts at: 3746 3898 6007 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 678 527 459 226 152 104
99 95 65
AseI AT'TA_AT
Cuts at: 160 1032 4765 160
Size: 872 3733 1606
Fragments arranged by size:
3733 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6211
AvaI C'yCGr_G
Cuts at: 1870 1943 3536 1870
Size: 73 1593 4545
Fragments arranged by size:
4545 1593 73
BamHI G'GATC_C
Cuts at: 3541 3908 3541
Size: 367 5844
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3031 3532 4987 618
Size: 325 217 627 1244 501 1455 1842
Fragments arranged by size:
1842 1455 1244 627 501 325 217
BanII G_rGCy'C
Cuts at: 1391 2127 2889 3530 1391
Size: 736 762 641 4072
Fragments arranged by size:
4072 762 736 641
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2299 928
Size: 971 400 4840
Fragments arranged by size:
4840 971 400
BbvCI CC'TCA_GC
Cuts at: 2367 2367
Size: 6211
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4402 4436 1903
Size: 34 2465 34 3678
Fragments arranged by size:
3678 2465 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4103 5313 4103
Size: 1210 5001
BclI T'GATC_A
Cuts at: 1548 2271 1548
Size: 723 5488
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4822 136
Size: 107 122 71 631 605 3150 1525
Fragments arranged by size:
3150 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6206 6206
Size: 6211
BlpI GC'TnA_GC
Cuts at: 3628 3628
Size: 6211
Bme1580I G_kGCm'C
Cuts at: 1790 2127 4272 5428 1790
Size: 337 2145 1156 2573
Fragments arranged by size:
2573 2145 1156 337
BmgBI CAC'GTC
Cuts at: 1318 2269 1318
Size: 951 5260
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2120 2327 2449
Size: 416 53 83 399 24 703 207 122
Cuts at: 2449 2690 2890 3101 3942 4900 442
Size: 241 200 211 841 958 1753
Fragments arranged by size:
1753 958 841 703 416 399 241 211
207 200 122 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6211
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3360 3360
Size: 6211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2903 4871 1239
Size: 1664 1968 2579
Fragments arranged by size:
2579 1968 1664
Bpu10I CC'TnA_GC
Cuts at: 2367 2367
Size: 6211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2414 2503 3143 4336 5201 5499 2414
Size: 89 640 1193 865 298 3126
Fragments arranged by size:
3126 1193 865 640 298 89
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2177 4874 882
Size: 617 678 2697 2219
Fragments arranged by size:
2697 2219 678 617
BsaAI yAC'GTr
Cuts at: 493 1204 4002 493
Size: 711 2798 2702
Fragments arranged by size:
2798 2702 711
BsaBI GATnn'nnATC
Cuts at: 6205 6205
Size: 6211
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4018 4400 5035 275
Size: 53 83 186 936 2485 382 635 1451
Fragments arranged by size:
2485 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4644 5042 5316 5463 5874 4644
Size: 398 274 147 411 4981
Fragments arranged by size:
4981 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5986
Fragments arranged by size:
5986 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 3254 1186
Size: 871 1197 4143
Fragments arranged by size:
4143 1197 871
BseYI C'CCAG_C
Cuts at: 5414 5910 5414
Size: 496 5715
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5638
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3661 4422 5450 1540
Size: 275 186 1660 761 1028 2301
Fragments arranged by size:
2301 1660 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2067 2624 2889 4272 4357 5428 2067
Size: 557 265 1383 85 1071 2850
Fragments arranged by size:
2850 1383 1071 557 265 85
BsmI GAATG_Cn'
Cuts at: 3722 3815 3722
Size: 93 6118
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2127 2624 2889 3530 4272 4357
Size: 399 277 60 497 265 641 742 85
Cuts at: 4357 5428 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 641 497 399 277 265
85 60
BspEI T'CCGG_A
Cuts at: 5874 5874
Size: 6211
BspHI T'CATG_A
Cuts at: 3348 4100 5830 3348
Size: 752 1730 3729
Fragments arranged by size:
3729 1730 752
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3566 844
Size: 2722 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4098 1394
Size: 429 2275 3507
Fragments arranged by size:
3507 2275 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2396 2448 4706 4880 65
Size: 1183 1148 52 2258 174 1396
Fragments arranged by size:
2258 1396 1183 1148 174 52
BsrFI r'CCGG_y
Cuts at: 1700 1810 4855 5042 1700
Size: 110 3045 187 2869
Fragments arranged by size:
3045 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6211
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6211
BssSI C'ACGA_G
Cuts at: 4271 5283 4271
Size: 1012 5199
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2995 794
Size: 2201 4010
BstBI TT'CG_AA
Cuts at: 2846 5015 2846
Size: 2169 4042
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6211
BstXI CCAn_nnnn'nTGG
Cuts at: 3225 3225
Size: 6211
BstYI r'GATC_y
Cuts at: 1972 2260 2653 3283 3417 3541 3908 4293 4310
Size: 288 393 630 134 124 367 385 17
Cuts at: 4310 5762 5848 5860 6206 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 630 393 385 367 346 288
134 124 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3237 3237
Size: 6211
BtgI C'CryG_G
Cuts at: 513 1065 2004 3051 513
Size: 552 939 1047 3673
Fragments arranged by size:
3673 1047 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5472
Fragments arranged by size:
5472 683 56
BtsI GCAGTG_nn'
Cuts at: 2944 3727 4521 4541 2944
Size: 783 794 20 4614
Fragments arranged by size:
4614 794 783 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6211
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6176
DraI TTT'AAA
Cuts at: 3515 3515
Size: 6211
DraIII CAC_nnn'GTG
Cuts at: 2193 2193
Size: 6211
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5218 809
Size: 507 3902 1802
Fragments arranged by size:
3902 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2250 2967 3658
Size: 54 1425 50 275 18 420 717 691
Cuts at: 3658 4547 6176 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 717 691 420 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3658 1537
Size: 275 1846 4090
Fragments arranged by size:
4090 1846 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2378 3210 4141 1353
Size: 1025 832 931 3423
Fragments arranged by size:
3423 1025 931 832
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3236 4798 5172 5318 387
Size: 1064 138 1647 1562 374 146 1280
Fragments arranged by size:
1647 1562 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2887 2887
Size: 6211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2804 2903 3365 4274 4871 5658 5926
Size: 393 1172 99 462 909 597 787 268
Cuts at: 5926 1239
Size: 1524
Fragments arranged by size:
1524 1172 909 787 597 462 393 268
99
EcoO109I rG'GnC_Cy
Cuts at: 2082 2123 2535 2082
Size: 41 412 5758
Fragments arranged by size:
5758 412 41
EcoRI G'AATT_C
Cuts at: 2115 2842 3520 3898 2115
Size: 727 678 378 4428
Fragments arranged by size:
4428 727 678 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3464 3464
Size: 6211
FspI TGC'GCA
Cuts at: 4717 4717
Size: 6211
HaeII r_GCGC'y
Cuts at: 1678 5358 1678
Size: 3680 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2565 2597 4852 4884 4926
Size: 32 135 32 993 32 2255 32 42
Cuts at: 4926 4958 1373
Size: 32 2626
Fragments arranged by size:
2626 2255 993 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2237 3555 3801 669
Size: 988 281 299 1318 246 3079
Fragments arranged by size:
3079 1318 988 299 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6211
HpaI GTT'AAC
Cuts at: 3801 3801
Size: 6211
KpnI G_GTAC'C
Cuts at: 3536 3536
Size: 6211
MfeI C'AATT_G
Cuts at: 3810 3810
Size: 6211
MluI A'CGCG_T
Cuts at: 6087 6087
Size: 6211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2432 3218 5325 5509 1305
Size: 1127 786 2107 184 2007
Fragments arranged by size:
2107 2007 1127 786 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2252 2969 10
Size: 54 1425 343 420 717 3252
Fragments arranged by size:
3252 1425 717 420 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2549 2979 3080 3332 3353 4169 4528
Size: 648 1383 430 101 252 21 816 359
Cuts at: 4528 4687 518
Size: 159 2042
Fragments arranged by size:
2042 1383 816 648 430 359 252 159
101 21
MspA1I CmG'CkG
Cuts at: 1606 2421 2824 3627 4304 5452 5697 1606
Size: 815 403 803 677 1148 245 2120
Fragments arranged by size:
2120 1148 815 803 677 403 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4720
Fragments arranged by size:
4720 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6211
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6211
NotI GC'GGCC_GC
Cuts at: 3658 3658
Size: 6211
NspI r_CATG'y
Cuts at: 1132 1229 3569 5114 1132
Size: 97 2340 1545 2229
Fragments arranged by size:
2340 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6211
PciI A'CATG_T
Cuts at: 5110 5110
Size: 6211
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6211
PmeI GTTT'AAAC
Cuts at: 3515 3515
Size: 6211
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2570 2602 4280 4312 5793 5825 2570
Size: 32 1678 32 1481 32 2956
Fragments arranged by size:
2956 1678 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2082 2082
Size: 6211
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6211
PsiI TTA'TAA
Cuts at: 3781 3781
Size: 6211
PspOMI G'GGCC_C
Cuts at: 2123 2123
Size: 6211
PstI C_TGCA'G
Cuts at: 830 3563 830
Size: 2733 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6211
PvuII CAG'CTG
Cuts at: 2824 2824
Size: 6211
SacI G_AGCT'C
Cuts at: 2889 2889
Size: 6211
SalI G'TCGA_C
Cuts at: 1936 3553 1936
Size: 1617 4594
SanDI GG'GwC_CC
Cuts at: 2082 2082
Size: 6211
SapI GCTCTTCn'nnn_
Cuts at: 3210 3210
Size: 6211
SbfI CC_TGCA'GG
Cuts at: 3563 3563
Size: 6211
ScaI AGT'ACT
Cuts at: 3224 4459 6171 3224
Size: 1235 1712 3264
Fragments arranged by size:
3264 1712 1235
SfcI C'TryA_G
Cuts at: 826 1045 2539 3559 4694 5375 5566 826
Size: 219 1494 1020 1135 681 191 1471
Fragments arranged by size:
1494 1471 1135 1020 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6211
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6211
SmaI CCC'GGG
Cuts at: 1872 3538 1872
Size: 1666 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2429 2482 3122 4315 5216 5478
Size: 197 926 486 53 640 1193 901 262
Cuts at: 5478 820
Size: 1553
Fragments arranged by size:
1553 1193 926 901 640 486 262 197
53
SnaBI TAC'GTA
Cuts at: 493 4002 493
Size: 3509 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6211
SphI G_CATG'C
Cuts at: 1132 3569 1132
Size: 2437 3774
SspI AAT'ATT
Cuts at: 5 52 4135 5
Size: 47 4083 2081
Fragments arranged by size:
4083 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6211
StyI C'CwwG_G
Cuts at: 513 1065 2004 3650 513
Size: 552 939 1646 3074
Fragments arranged by size:
3074 1646 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4238 4255 4408 4593 5038 325
Size: 1255 2658 17 153 185 445 1498
Fragments arranged by size:
2658 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3222 4457 6169
Size: 274 80 33 51 1443 1245 1235 1712
Cuts at: 6169 96
Size: 138
Fragments arranged by size:
1712 1443 1245 1235 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6171 6203 6171
Size: 32 6179
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6211
XbaI T'CTAG_A
Cuts at: 3547 3547
Size: 6211
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3178 3178
Size: 6211
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6211
XmaI C'CCGG_G
Cuts at: 1870 3536 1870
Size: 1666 4545
XmnI GAAnn'nnTTC
Cuts at: 4340 4340
Size: 6211
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4019 276
Size: 53 83 186 936 2485 2468
Fragments arranged by size:
2485 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI
SwaI