Restriction map for
pFN21AE4484
(Circular) (Six-base) MAPSORT of: pFN21AE4484.seq Check: 7034 from: 1 to: 6205
pFN21AE4484 6205 bp
With 182 enzymes: SgfI *
March 28, 2012 13:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4015 278
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3548 1937
Size: 1611 4594
Acc65I G'GTAC_C
Cuts at: 3526 3526
Size: 6205
AclI AA'CG_TT
Cuts at: 1563 4332 4705 1563
Size: 2769 373 3063
Fragments arranged by size:
3063 2769 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4268 5652 5920 1632
Size: 2636 1384 268 1917
Fragments arranged by size:
2636 1917 1384 268
AfeI AGC'GCT
Cuts at: 3172 3172
Size: 6205
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6008
AflIII A'CryG_T
Cuts at: 1203 3246 5104 6081 1203
Size: 2043 1858 977 1327
Fragments arranged by size:
2043 1858 1327 977
AgeI A'CCGG_T
Cuts at: 5036 5036
Size: 6205
AhdI GACnn_n'nnGTC
Cuts at: 4934 4934
Size: 6205
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6205
AlwNI CAG_nnn'CTG
Cuts at: 2761 3948 5520 2761
Size: 1187 1572 3446
Fragments arranged by size:
3446 1572 1187
ApaI G_GGCC'C
Cuts at: 2604 3483 2604
Size: 879 5326
ApaLI G'TGCA_C
Cuts at: 4262 5418 4262
Size: 1156 5049
ApoI r'AATT_y
Cuts at: 1493 2988 3514 3740 3892 6001 1493
Size: 1495 526 226 152 2109 1697
Fragments arranged by size:
2109 1697 1495 526 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2751 4759 160
Size: 872 1719 2008 1606
Fragments arranged by size:
2008 1719 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
AvaI C'yCGr_G
Cuts at: 1870 1943 3530 1870
Size: 73 1587 4545
Fragments arranged by size:
4545 1587 73
BamHI G'GATC_C
Cuts at: 3535 3902 3535
Size: 367 5838
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2488 3526 4981 618
Size: 325 217 627 701 1038 1455 1842
Fragments arranged by size:
1842 1455 1038 701 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2584 2604 3483 3524 1391
Size: 1193 20 879 41 4072
Fragments arranged by size:
4072 1193 879 41 20
BbeI G_GCGC'C
Cuts at: 2492 2492
Size: 6205
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2122 2716 2731 928
Size: 971 223 594 15 4402
Fragments arranged by size:
4402 971 594 223 15
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4396 4430 1903
Size: 34 50 34 2375 34 3678
Fragments arranged by size:
3678 2375 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2027 2246 4097 5307 2027
Size: 219 1851 1210 2925
Fragments arranged by size:
2925 1851 1210 219
BclI T'GATC_A
Cuts at: 1548 2817 1548
Size: 1269 4936
BfrBI ATG'CAT
Cuts at: 2108 2108
Size: 6205
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4816 136
Size: 107 122 71 631 605 3144 1525
Fragments arranged by size:
3144 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2677 2698 6200 2677
Size: 21 3502 2682
Fragments arranged by size:
3502 2682 21
BlpI GC'TnA_GC
Cuts at: 3113 3622 3113
Size: 509 5696
Bme1580I G_kGCm'C
Cuts at: 1790 2604 3483 4266 5422 1790
Size: 814 879 783 1156 2573
Fragments arranged by size:
2573 1156 879 814 783
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6205
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3283 3936 4894
Size: 416 53 83 399 24 1866 653 958
Cuts at: 4894 442
Size: 1753
Fragments arranged by size:
1866 1753 958 653 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3044 1056
Size: 1988 4217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2598 3151 3438 4865 1239
Size: 1359 553 287 1427 2579
Fragments arranged by size:
2579 1427 1359 553 287
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2666 4330 5195 5493 2666
Size: 1664 865 298 3378
Fragments arranged by size:
3378 1664 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3392 4868 882
Size: 617 1893 1476 2219
Fragments arranged by size:
2219 1893 1476 617
BsaAI yAC'GTr
Cuts at: 493 1204 3996 493
Size: 711 2792 2702
Fragments arranged by size:
2792 2702 711
BsaBI GATnn'nnATC
Cuts at: 6199 6199
Size: 6205
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2489 3285 4012 4394
Size: 53 83 186 936 956 796 727 382
Cuts at: 4394 5029 275
Size: 635 1451
Fragments arranged by size:
1451 956 936 796 727 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4638 5036 5310 5457 5868 4638
Size: 398 274 147 411 4975
Fragments arranged by size:
4975 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2013 2043 1178
Size: 30 165 30 610 30 5340
Fragments arranged by size:
5340 610 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3503 1186
Size: 2317 3888
BseYI C'CCAG_C
Cuts at: 2055 2061 3416 5408 5904 2055
Size: 6 1355 1992 496 2356
Fragments arranged by size:
2356 1992 1355 496 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5632
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3655 4416 5444 1540
Size: 275 186 1654 761 1028 2301
Fragments arranged by size:
2301 1654 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4266 4351 5422 4266
Size: 85 1071 5049
Fragments arranged by size:
5049 1071 85
BsmI GAATG_Cn'
Cuts at: 3203 3716 3809 3203
Size: 513 93 5599
Fragments arranged by size:
5599 513 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2584 2604 3483 3524 4266 4351 5422
Size: 399 794 20 879 41 742 85 1071
Cuts at: 5422 1391
Size: 2174
Fragments arranged by size:
2174 1071 879 794 742 399 85 41
20
BspEI T'CCGG_A
Cuts at: 5868 5868
Size: 6205
BspHI T'CATG_A
Cuts at: 2268 4094 5824 2268
Size: 1826 1730 2649
Fragments arranged by size:
2649 1826 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3560 844
Size: 2716 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4092 1394
Size: 429 2269 3507
Fragments arranged by size:
3507 2269 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4700 4874 65
Size: 1183 3452 174 1396
Fragments arranged by size:
3452 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4849 5036 1700
Size: 110 3039 187 2869
Fragments arranged by size:
3039 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6205
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6205
BssSI C'ACGA_G
Cuts at: 4265 5277 4265
Size: 1012 5193
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6205
BstBI TT'CG_AA
Cuts at: 5009 5009
Size: 6205
BstEII G'GTnAC_C
Cuts at: 1184 3184 1184
Size: 2000 4205
BstXI CCAn_nnnn'nTGG
Cuts at: 2200 2449 3175 2200
Size: 249 726 5230
Fragments arranged by size:
5230 726 249
BstYI r'GATC_y
Cuts at: 1972 2677 2698 3535 3902 4287 4304 5756 5842
Size: 705 21 837 367 385 17 1452 86
Cuts at: 5842 5854 6200 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 837 705 385 367 346 86
21 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2454 513
Size: 552 1389 4264
Fragments arranged by size:
4264 1389 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5466
Fragments arranged by size:
5466 683 56
BtsI GCAGTG_nn'
Cuts at: 2073 3721 4515 4535 2073
Size: 1648 794 20 3743
Fragments arranged by size:
3743 1648 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6205
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6170
DraI TTT'AAA
Cuts at: 3509 3509
Size: 6205
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5212 809
Size: 507 3896 1802
Fragments arranged by size:
3896 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2439 2451 3652
Size: 54 1425 50 275 18 609 12 1201
Cuts at: 3652 4541 6170 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1201 889 609 275 54 50
43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 3652 1537
Size: 275 1840 4090
Fragments arranged by size:
4090 1840 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2864 3320 3390 4135 1353
Size: 1511 456 70 745 3423
Fragments arranged by size:
3423 1511 745 456 70
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4792 5166 5312 387
Size: 1064 138 3203 374 146 1280
Fragments arranged by size:
3203 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2598 3151 3438 4268 4865 5652 5920
Size: 393 966 553 287 830 597 787 268
Cuts at: 5920 1239
Size: 1524
Fragments arranged by size:
1524 966 830 787 597 553 393 287
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2498 2498
Size: 6205
EcoO109I rG'GnC_Cy
Cuts at: 2222 3479 2222
Size: 1257 4948
EcoRI G'AATT_C
Cuts at: 2988 3514 3892 2988
Size: 526 378 5301
Fragments arranged by size:
5301 526 378
EcoRV GAT'ATC
Cuts at: 2187 2187
Size: 6205
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2660 2917 2660
Size: 257 5948
FspI TGC'GCA
Cuts at: 4711 4711
Size: 6205
HaeII r_GCGC'y
Cuts at: 1678 2492 3174 5352 1678
Size: 814 682 2178 2531
Fragments arranged by size:
2531 2178 814 682
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2211 2243 2717 2749 3060
Size: 32 135 32 639 32 474 32 311
Cuts at: 3060 3092 4846 4878 4920 4952 1373
Size: 32 1754 32 42 32 2626
Fragments arranged by size:
2626 1754 639 474 311 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2929 3549 3795 669
Size: 988 281 991 620 246 3079
Fragments arranged by size:
3079 991 988 620 281 246
HindIII A'AGCT_T
Cuts at: 748 2533 748
Size: 1785 4420
HpaI GTT'AAC
Cuts at: 3795 3795
Size: 6205
KasI G'GCGC_C
Cuts at: 2488 2488
Size: 6205
KpnI G_GTAC'C
Cuts at: 3530 3530
Size: 6205
MfeI C'AATT_G
Cuts at: 3804 3804
Size: 6205
MluI A'CGCG_T
Cuts at: 6081 6081
Size: 6205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1999 2011 3046 3130 5319 5503 1305
Size: 694 12 1035 84 2189 184 2007
Fragments arranged by size:
2189 2007 1035 694 184 84 12
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2441 2453 10
Size: 54 1425 343 609 12 3762
Fragments arranged by size:
3762 1425 609 343 54 12
MslI CAynn'nnrTG
Cuts at: 518 1166 3323 4163 4522 4681 518
Size: 648 2157 840 359 159 2042
Fragments arranged by size:
2157 2042 840 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3621 4298 5446 5691 1606
Size: 2015 677 1148 245 2120
Fragments arranged by size:
2120 2015 1148 677 245
NarI GG'CG_CC
Cuts at: 2489 2489
Size: 6205
NcoI C'CATG_G
Cuts at: 513 1065 2454 513
Size: 552 1389 4264
Fragments arranged by size:
4264 1389 552
NdeI CA'TA_TG
Cuts at: 387 3036 387
Size: 2649 3556
NheI G'CTAG_C
Cuts at: 1052 3040 1052
Size: 1988 4217
NotI GC'GGCC_GC
Cuts at: 3652 3652
Size: 6205
NsiI A_TGCA'T
Cuts at: 2110 2110
Size: 6205
NspI r_CATG'y
Cuts at: 1132 1229 3250 3282 3563 5108 1132
Size: 97 2021 32 281 1545 2229
Fragments arranged by size:
2229 2021 1545 281 97 32
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6205
PciI A'CATG_T
Cuts at: 3246 5104 3246
Size: 1858 4347
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2018 1108
Size: 910 5295
PfoI T'CCnGG_A
Cuts at: 2760 2760
Size: 6205
PmeI GTTT'AAAC
Cuts at: 3509 3509
Size: 6205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4274 4306 5787 5819 4274
Size: 32 1481 32 4660
Fragments arranged by size:
4660 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2222 2222
Size: 6205
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6205
PsiI TTA'TAA
Cuts at: 3775 3775
Size: 6205
PspOMI G'GGCC_C
Cuts at: 2600 3479 2600
Size: 879 5326
PstI C_TGCA'G
Cuts at: 830 3557 830
Size: 2727 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6205
SalI G'TCGA_C
Cuts at: 1936 3547 1936
Size: 1611 4594
SbfI CC_TGCA'GG
Cuts at: 3557 3557
Size: 6205
ScaI AGT'ACT
Cuts at: 4453 6165 4453
Size: 1712 4493
SfcI C'TryA_G
Cuts at: 826 1045 3553 4688 5369 5560 826
Size: 219 2508 1135 681 191 1471
Fragments arranged by size:
2508 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2490 2490
Size: 6205
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6205
SmaI CCC'GGG
Cuts at: 1872 3532 1872
Size: 1660 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2645 4309 5210 5472 820
Size: 197 926 702 1664 901 262 1553
Fragments arranged by size:
1664 1553 926 901 702 262 197
SnaBI TAC'GTA
Cuts at: 493 3996 493
Size: 3503 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6205
SphI G_CATG'C
Cuts at: 1132 3563 1132
Size: 2431 3774
SspI AAT'ATT
Cuts at: 5 52 4129 5
Size: 47 4077 2081
Fragments arranged by size:
4077 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6205
StyI C'CwwG_G
Cuts at: 513 1065 2454 2613 3483 3644 513
Size: 552 1389 159 870 161 3074
Fragments arranged by size:
3074 1389 870 552 161 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2795 4232 4249 4402 4587 5032 325
Size: 1255 1215 1437 17 153 185 445 1498
Fragments arranged by size:
1498 1437 1255 1215 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2480 4451 6163
Size: 274 80 33 51 1443 503 1971 1712
Cuts at: 6163 96
Size: 138
Fragments arranged by size:
1971 1712 1443 503 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6165 6197 6165
Size: 32 6173
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6205
XbaI T'CTAG_A
Cuts at: 3541 3541
Size: 6205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2052 2052
Size: 6205
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6205
XmaI C'CCGG_G
Cuts at: 1870 3530 1870
Size: 1660 4545
XmnI GAAnn'nnTTC
Cuts at: 4334 4334
Size: 6205
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4013 276
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BplI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI
NaeI NgoMIV NruI PacI PmlI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI