Restriction map for
pFN21AE4483
(Circular) (Six-base) MAPSORT of: pFN21AE4483.seq Check: 5439 from: 1 to: 6205
pFN21AE4483 6205 bp
With 182 enzymes: SgfI *
March 28, 2012 13:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4015 278
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3204 3548 1937
Size: 1267 344 4594
Fragments arranged by size:
4594 1267 344
Acc65I G'GTAC_C
Cuts at: 3526 3526
Size: 6205
AclI AA'CG_TT
Cuts at: 1563 4332 4705 1563
Size: 2769 373 3063
Fragments arranged by size:
3063 2769 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3055 4268 5652 5920 1632
Size: 1423 1213 1384 268 1917
Fragments arranged by size:
1917 1423 1384 1213 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6008
AflIII A'CryG_T
Cuts at: 1203 2672 5104 6081 1203
Size: 1469 2432 977 1327
Fragments arranged by size:
2432 1469 1327 977
AgeI A'CCGG_T
Cuts at: 5036 5036
Size: 6205
AhdI GACnn_n'nnGTC
Cuts at: 4934 4934
Size: 6205
AleI CACnn'nnGTG
Cuts at: 1166 2807 1166
Size: 1641 4564
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6205
AlwNI CAG_nnn'CTG
Cuts at: 3148 3948 5520 3148
Size: 800 1572 3833
Fragments arranged by size:
3833 1572 800
ApaI G_GGCC'C
Cuts at: 2595 2595
Size: 6205
ApaLI G'TGCA_C
Cuts at: 2893 4262 5418 2893
Size: 1369 1156 3680
Fragments arranged by size:
3680 1369 1156
ApoI r'AATT_y
Cuts at: 1493 3514 3740 3892 6001 1493
Size: 2021 226 152 2109 1697
Fragments arranged by size:
2109 2021 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4759 160
Size: 872 3727 1606
Fragments arranged by size:
3727 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
AvaI C'yCGr_G
Cuts at: 1870 1943 2483 3018 3530 1870
Size: 73 540 535 512 4545
Fragments arranged by size:
4545 540 535 512 73
BamHI G'GATC_C
Cuts at: 2581 3535 3902 2581
Size: 954 367 4884
Fragments arranged by size:
4884 954 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3526 4981 618
Size: 325 217 627 1739 1455 1842
Fragments arranged by size:
1842 1739 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2069 2595 3102 3438 3524 1391
Size: 678 526 507 336 86 4072
Fragments arranged by size:
4072 678 526 507 336 86
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2235 3061 3405 928
Size: 971 336 826 344 3728
Fragments arranged by size:
3728 971 826 344 336
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3164 3198 4396 4430 1903
Size: 34 1227 34 1198 34 3678
Fragments arranged by size:
3678 1227 1198 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4097 5307 4097
Size: 1210 4995
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6205
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4816 136
Size: 107 122 71 631 605 3144 1525
Fragments arranged by size:
3144 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6200 6200
Size: 6205
BlpI GC'TnA_GC
Cuts at: 3622 3622
Size: 6205
Bme1580I G_kGCm'C
Cuts at: 1790 2595 2897 4266 5422 1790
Size: 805 302 1369 1156 2573
Fragments arranged by size:
2573 1369 1156 805 302
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6205
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3936 4894 442
Size: 416 53 83 399 24 2519 958 1753
Fragments arranged by size:
2519 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6205
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2646 3277 2646
Size: 631 5574
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2056 3484 4865 1239
Size: 817 1428 1381 2579
Fragments arranged by size:
2579 1428 1381 817
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2707 4330 5195 5493 2707
Size: 1623 865 298 3419
Fragments arranged by size:
3419 1623 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3219 4868 882
Size: 617 1720 1649 2219
Fragments arranged by size:
2219 1720 1649 617
BsaAI yAC'GTr
Cuts at: 493 1204 2524 3996 493
Size: 711 1320 1472 2702
Fragments arranged by size:
2702 1472 1320 711
BsaBI GATnn'nnATC
Cuts at: 3059 6199 3059
Size: 3140 3065
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2349 4012 4394 5029
Size: 53 83 186 936 816 1663 382 635
Cuts at: 5029 275
Size: 1451
Fragments arranged by size:
1663 1451 936 816 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2632 4638 5036 5310 5457 5868 2632
Size: 2006 398 274 147 411 2969
Fragments arranged by size:
2969 2006 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2508 2538 3425 3455 1178
Size: 30 165 30 1105 30 887 30 3928
Fragments arranged by size:
3928 1105 887 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3227 1186
Size: 2041 4164
BseYI C'CCAG_C
Cuts at: 3372 5408 5904 3372
Size: 2036 496 3673
Fragments arranged by size:
3673 2036 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2294 2315 1163
Size: 573 558 21 5053
Fragments arranged by size:
5053 573 558 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3655 4416 5444 1540
Size: 275 186 1654 761 1028 2301
Fragments arranged by size:
2301 1654 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2897 3438 4266 4351 5422 2897
Size: 541 828 85 1071 3680
Fragments arranged by size:
3680 1071 828 541 85
BsmI GAATG_Cn'
Cuts at: 3187 3716 3809 3187
Size: 529 93 5583
Fragments arranged by size:
5583 529 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 2595 2897 3102 3438 3524 4266
Size: 399 279 526 302 205 336 86 742
Cuts at: 4266 4351 5422 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 526 399 336 302 279
205 86 85
BspEI T'CCGG_A
Cuts at: 2632 5868 2632
Size: 3236 2969
BspHI T'CATG_A
Cuts at: 4094 5824 4094
Size: 1730 4475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3560 844
Size: 2716 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2289 3169 4092 1394
Size: 429 466 880 923 3507
Fragments arranged by size:
3507 923 880 466 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2436 4700 4874 65
Size: 1183 1188 2264 174 1396
Fragments arranged by size:
2264 1396 1188 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4849 5036 1700
Size: 110 3039 187 2869
Fragments arranged by size:
3039 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3392 96
Size: 3296 2909
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6205
BssSI C'ACGA_G
Cuts at: 2318 4265 5277 2318
Size: 1947 1012 3246
Fragments arranged by size:
3246 1947 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6205
BstBI TT'CG_AA
Cuts at: 5009 5009
Size: 6205
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6205
BstXI CCAn_nnnn'nTGG
Cuts at: 2425 2425
Size: 6205
BstYI r'GATC_y
Cuts at: 1972 2581 2922 3237 3535 3902 4287 4304 5756
Size: 609 341 315 298 367 385 17 1452
Cuts at: 5756 5842 5854 6200 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 609 385 367 346 341 315
298 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5653
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5466
Fragments arranged by size:
5466 683 56
BtsI GCAGTG_nn'
Cuts at: 3721 4515 4535 3721
Size: 794 20 5391
Fragments arranged by size:
5391 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6205
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6170
DraI TTT'AAA
Cuts at: 3509 3509
Size: 6205
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5212 809
Size: 507 3896 1802
Fragments arranged by size:
3896 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2209 2526 3652
Size: 54 1425 50 275 18 379 317 1126
Cuts at: 3652 4541 6170 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1126 889 379 317 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3652 1537
Size: 275 1840 4090
Fragments arranged by size:
4090 1840 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4135 1353
Size: 2782 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4792 5166 5312 387
Size: 1064 138 3203 374 146 1280
Fragments arranged by size:
3203 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3436 3436
Size: 6205
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2056 3055 3484 4268 4865 5652 5920
Size: 393 424 999 429 784 597 787 268
Cuts at: 5920 1239
Size: 1524
Fragments arranged by size:
1524 999 787 784 597 429 424 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 3284 3284
Size: 6205
EcoO109I rG'GnC_Cy
Cuts at: 2176 3258 2176
Size: 1082 5123
EcoRI G'AATT_C
Cuts at: 3514 3892 3514
Size: 378 5827
FspI TGC'GCA
Cuts at: 4711 4711
Size: 6205
HaeII r_GCGC'y
Cuts at: 1678 5352 1678
Size: 3674 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2184 2216 4846 4878 4920
Size: 32 135 32 612 32 2630 32 42
Cuts at: 4920 4952 1373
Size: 32 2626
Fragments arranged by size:
2630 2626 612 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2368 2851 3549 3795 669
Size: 988 281 430 483 698 246 3079
Fragments arranged by size:
3079 988 698 483 430 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6205
HpaI GTT'AAC
Cuts at: 3795 3795
Size: 6205
KpnI G_GTAC'C
Cuts at: 3530 3530
Size: 6205
MfeI C'AATT_G
Cuts at: 2784 3804 2784
Size: 1020 5185
MluI A'CGCG_T
Cuts at: 2672 6081 2672
Size: 3409 2796
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5319 5503 1305
Size: 4014 184 2007
Fragments arranged by size:
4014 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4383
Fragments arranged by size:
4383 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2807 3353 4163 4522 4681 518
Size: 648 1641 546 810 359 159 2042
Fragments arranged by size:
2042 1641 810 648 546 359 159
MspA1I CmG'CkG
Cuts at: 1606 3376 3621 4298 5446 5691 1606
Size: 1770 245 677 1148 245 2120
Fragments arranged by size:
2120 1770 1148 677 245 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5653
NdeI CA'TA_TG
Cuts at: 387 2791 387
Size: 2404 3801
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6205
NotI GC'GGCC_GC
Cuts at: 3652 3652
Size: 6205
NspI r_CATG'y
Cuts at: 1132 1229 3031 3563 5108 1132
Size: 97 1802 532 1545 2229
Fragments arranged by size:
2229 1802 1545 532 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6205
PciI A'CATG_T
Cuts at: 5104 5104
Size: 6205
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2759 1108
Size: 1651 4554
PmeI GTTT'AAAC
Cuts at: 3509 3509
Size: 6205
PmlI CAC'GTG
Cuts at: 2524 2524
Size: 6205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4274 4306 5787 5819 4274
Size: 32 1481 32 4660
Fragments arranged by size:
4660 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2176 2176
Size: 6205
PshAI GACnn'nnGTC
Cuts at: 1613 2135 1613
Size: 522 5683
PsiI TTA'TAA
Cuts at: 3775 3775
Size: 6205
PspOMI G'GGCC_C
Cuts at: 2591 2591
Size: 6205
PstI C_TGCA'G
Cuts at: 830 2237 2463 3557 830
Size: 1407 226 1094 3478
Fragments arranged by size:
3478 1407 1094 226
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6205
SacI G_AGCT'C
Cuts at: 3438 3438
Size: 6205
SalI G'TCGA_C
Cuts at: 1936 3547 1936
Size: 1611 4594
SbfI CC_TGCA'GG
Cuts at: 3557 3557
Size: 6205
ScaI AGT'ACT
Cuts at: 4453 6165 4453
Size: 1712 4493
SfcI C'TryA_G
Cuts at: 826 1045 2233 2459 2506 3553 4688 5369 5560
Size: 219 1188 226 47 1047 1135 681 191
Cuts at: 5560 826
Size: 1471
Fragments arranged by size:
1471 1188 1135 1047 681 226 219 191
47
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6205
SmaI CCC'GGG
Cuts at: 1872 3020 3532 1872
Size: 1148 512 4545
Fragments arranged by size:
4545 1148 512
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2686 4309 5210 5472 820
Size: 197 926 743 1623 901 262 1553
Fragments arranged by size:
1623 1553 926 901 743 262 197
SnaBI TAC'GTA
Cuts at: 493 3996 493
Size: 3503 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6205
SphI G_CATG'C
Cuts at: 1132 3031 3563 1132
Size: 1899 532 3774
Fragments arranged by size:
3774 1899 532
SspI AAT'ATT
Cuts at: 5 52 4129 5
Size: 47 4077 2081
Fragments arranged by size:
4077 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6205
StyI C'CwwG_G
Cuts at: 513 1065 2172 3644 513
Size: 552 1107 1472 3074
Fragments arranged by size:
3074 1472 1107 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4232 4249 4402 4587 5032 325
Size: 1255 2652 17 153 185 445 1498
Fragments arranged by size:
2652 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3392 4451 6163
Size: 274 80 33 51 1443 1415 1059 1712
Cuts at: 6163 96
Size: 138
Fragments arranged by size:
1712 1443 1415 1059 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2508 2540 6165 6197 2508
Size: 32 3625 32 2516
Fragments arranged by size:
3625 2516 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6205
XbaI T'CTAG_A
Cuts at: 3541 3541
Size: 6205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2359 2756 3196 3490 2359
Size: 397 440 294 5074
Fragments arranged by size:
5074 440 397 294
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6205
XmaI C'CCGG_G
Cuts at: 1870 3018 3530 1870
Size: 1148 512 4545
Fragments arranged by size:
4545 1148 512
XmnI GAAnn'nnTTC
Cuts at: 4334 4334
Size: 6205
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4013 276
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PspXI PsrI PvuII RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI