Restriction map for
pFN21AE4481
(Circular) (Six-base) MAPSORT of: pFN21AE4481.seq Check: 5656 from: 1 to: 6199
pFN21AE4481 6199 bp
With 182 enzymes: SgfI *
March 28, 2012 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4009 278
Size: 53 83 186 936 2473 2468
Fragments arranged by size:
2473 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3542 1937
Size: 1605 4594
Acc65I G'GTAC_C
Cuts at: 2383 3520 2383
Size: 1137 5062
AclI AA'CG_TT
Cuts at: 1563 4326 4699 1563
Size: 2763 373 3063
Fragments arranged by size:
3063 2763 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3000 3101 4262 5646 5914 1632
Size: 1368 101 1161 1384 268 1917
Fragments arranged by size:
1917 1384 1368 1161 268 101
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6002
AflIII A'CryG_T
Cuts at: 1203 2064 2529 3159 5098 6075 1203
Size: 861 465 630 1939 977 1327
Fragments arranged by size:
1939 1327 977 861 630 465
AgeI A'CCGG_T
Cuts at: 2380 5030 2380
Size: 2650 3549
AhdI GACnn_n'nnGTC
Cuts at: 2020 4928 2020
Size: 2908 3291
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6199
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3258 1184
Size: 2074 4125
AlwNI CAG_nnn'CTG
Cuts at: 2044 2898 3473 3942 5514 2044
Size: 854 575 469 1572 2729
Fragments arranged by size:
2729 1572 854 575 469
ApaI G_GGCC'C
Cuts at: 2324 2324
Size: 6199
ApaLI G'TGCA_C
Cuts at: 4256 5412 4256
Size: 1156 5043
ApoI r'AATT_y
Cuts at: 1493 2182 2232 2939 3508 3734 3886 5995 1493
Size: 689 50 707 569 226 152 2109 1697
Fragments arranged by size:
2109 1697 707 689 569 226 152 50
AseI AT'TA_AT
Cuts at: 160 1032 3065 4753 160
Size: 872 2033 1688 1606
Fragments arranged by size:
2033 1688 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6199
AvaI C'yCGr_G
Cuts at: 1870 1943 2591 3141 3524 1870
Size: 73 648 550 383 4545
Fragments arranged by size:
4545 648 550 383 73
BamHI G'GATC_C
Cuts at: 3529 3896 3529
Size: 367 5832
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2192 2383 3520 4975 618
Size: 325 217 627 405 191 1137 1455 1842
Fragments arranged by size:
1842 1455 1137 627 405 325 217 191
BanII G_rGCy'C
Cuts at: 1391 2324 2611 3179 3518 1391
Size: 933 287 568 339 4072
Fragments arranged by size:
4072 933 568 339 287
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2652 928
Size: 971 753 4475
Fragments arranged by size:
4475 971 753
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4390 4424 1903
Size: 34 2453 34 3678
Fragments arranged by size:
3678 2453 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4091 5301 4091
Size: 1210 4989
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6199
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4810 136
Size: 107 122 71 631 605 3138 1525
Fragments arranged by size:
3138 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6194 6194
Size: 6199
BlpI GC'TnA_GC
Cuts at: 3616 3616
Size: 6199
Bme1580I G_kGCm'C
Cuts at: 1790 2324 4260 5416 1790
Size: 534 1936 1156 2573
Fragments arranged by size:
2573 1936 1156 534
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6199
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2250 2786 3930
Size: 416 53 83 399 24 833 536 1144
Cuts at: 3930 4888 442
Size: 958 1753
Fragments arranged by size:
1753 1144 958 833 536 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2142 3084 4859 1239
Size: 903 942 1775 2579
Fragments arranged by size:
2579 1775 942 903
Bpu10I CC'TnA_GC
Cuts at: 2874 3203 2874
Size: 329 5870
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2501 2518 2971 3389 4324 5189 5487 2501
Size: 17 453 418 935 865 298 3213
Fragments arranged by size:
3213 935 865 453 418 298 17
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3019 4862 882
Size: 617 1520 1843 2219
Fragments arranged by size:
2219 1843 1520 617
BsaAI yAC'GTr
Cuts at: 493 1204 2067 2551 3990 493
Size: 711 863 484 1439 2702
Fragments arranged by size:
2702 1439 863 711 484
BsaBI GATnn'nnATC
Cuts at: 6193 6193
Size: 6199
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3296 4006 4388 5023
Size: 53 83 186 936 1763 710 382 635
Cuts at: 5023 275
Size: 1451
Fragments arranged by size:
1763 1451 936 710 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2186 2380 4632 5030 5304 5451 5862 2186
Size: 194 2252 398 274 147 411 2523
Fragments arranged by size:
2523 2252 411 398 274 194 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5974
Fragments arranged by size:
5974 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2105 2108 1186
Size: 919 3 5277
Fragments arranged by size:
5277 919 3
BseYI C'CCAG_C
Cuts at: 2323 2698 5402 5898 2323
Size: 375 2704 496 2624
Fragments arranged by size:
2704 2624 496 375
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5626
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3649 4410 5438 1540
Size: 275 186 1648 761 1028 2301
Fragments arranged by size:
2301 1648 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2106 2517 3150 4260 4345 5416 2106
Size: 411 633 1110 85 1071 2889
Fragments arranged by size:
2889 1110 1071 633 411 85
BsiWI C'GTAC_G
Cuts at: 2443 2443
Size: 6199
BsmI GAATG_Cn'
Cuts at: 3710 3803 3710
Size: 93 6106
BsmBI CGTCTCn'nnnn_
Cuts at: 2090 3287 2090
Size: 1197 5002
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2324 2517 2611 3150 3179 3518
Size: 399 316 218 193 94 539 29 339
Cuts at: 3518 4260 4345 5416 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 539 399 339 316 218
193 94 85 29
BspEI T'CCGG_A
Cuts at: 2186 5862 2186
Size: 3676 2523
BspHI T'CATG_A
Cuts at: 3312 4088 5818 3312
Size: 776 1730 3693
Fragments arranged by size:
3693 1730 776
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3554 844
Size: 2710 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4086 1394
Size: 429 2263 3507
Fragments arranged by size:
3507 2263 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2301 4694 4868 65
Size: 1183 1053 2393 174 1396
Fragments arranged by size:
2393 1396 1183 1053 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2380 4843 5030 1700
Size: 110 570 2463 187 2869
Fragments arranged by size:
2869 2463 570 187 110
BsrGI T'GTAC_A
Cuts at: 96 2850 3444 96
Size: 2754 594 2851
Fragments arranged by size:
2851 2754 594
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6199
BssSI C'ACGA_G
Cuts at: 4259 5271 4259
Size: 1012 5187
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2275 794
Size: 1481 4718
BstBI TT'CG_AA
Cuts at: 2246 5003 2246
Size: 2757 3442
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6199
BstXI CCAn_nnnn'nTGG
Cuts at: 2625 2926 2625
Size: 301 5898
BstYI r'GATC_y
Cuts at: 1972 2835 3529 3896 4281 4298 5750 5836 5848
Size: 863 694 367 385 17 1452 86 12
Cuts at: 5848 6194 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 863 694 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2856 3225 513
Size: 552 939 852 369 3487
Fragments arranged by size:
3487 939 852 552 369
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5460
Fragments arranged by size:
5460 683 56
BtsI GCAGTG_nn'
Cuts at: 2642 3715 4509 4529 2642
Size: 1073 794 20 4312
Fragments arranged by size:
4312 1073 794 20
ClaI AT'CG_AT
Cuts at: 1554 2905 1554
Size: 1351 4848
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6164
DraI TTT'AAA
Cuts at: 3503 3503
Size: 6199
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5206 809
Size: 507 3890 1802
Fragments arranged by size:
3890 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 3646 4535
Size: 54 1425 50 275 18 198 1618 889
Cuts at: 4535 6164 8
Size: 1629 43
Fragments arranged by size:
1629 1618 1425 889 275 198 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3646 1537
Size: 275 1834 4090
Fragments arranged by size:
4090 1834 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2156 2812 3108 4129 1353
Size: 803 656 296 1021 3423
Fragments arranged by size:
3423 1021 803 656 296
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4786 5160 5306 387
Size: 1064 138 435 2762 374 146 1280
Fragments arranged by size:
2762 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2142 3000 3084 3101 4262 4859 5646
Size: 393 510 858 84 17 1161 597 787
Cuts at: 5646 5914 1239
Size: 268 1524
Fragments arranged by size:
1524 1161 858 787 597 510 393 268
84 17
EcoO109I rG'GnC_Cy
Cuts at: 2320 2320
Size: 6199
EcoRI G'AATT_C
Cuts at: 3508 3886 3508
Size: 378 5821
EcoRV GAT'ATC
Cuts at: 2284 3046 2284
Size: 762 5437
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3409 3409
Size: 6199
FspI TGC'GCA
Cuts at: 4705 4705
Size: 6199
HaeII r_GCGC'y
Cuts at: 1678 5346 1678
Size: 3668 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2006 2038 2195 2227 2291
Size: 32 135 32 434 32 157 32 64
Cuts at: 2291 2323 3035 3051 3067 3083 4840 4872 4914
Size: 32 712 16 16 16 1757 32 42
Cuts at: 4914 4946 1373
Size: 32 2626
Fragments arranged by size:
2626 1757 712 434 157 135 64 42
32 32 32 32 32 32 32 16
16 16
HincII GTy'rAC
Cuts at: 669 1657 1938 3543 3789 669
Size: 988 281 1605 246 3079
Fragments arranged by size:
3079 1605 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6199
HpaI GTT'AAC
Cuts at: 3789 3789
Size: 6199
KpnI G_GTAC'C
Cuts at: 2387 3524 2387
Size: 1137 5062
MfeI C'AATT_G
Cuts at: 2421 3448 3798 2421
Size: 1027 350 4822
Fragments arranged by size:
4822 1027 350
MluI A'CGCG_T
Cuts at: 6075 6075
Size: 6199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3463 5313 5497 1305
Size: 2158 1850 184 2007
Fragments arranged by size:
2158 2007 1850 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4377
Fragments arranged by size:
4377 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3230 4157 4516 4675 518
Size: 648 2064 927 359 159 2042
Fragments arranged by size:
2064 2042 927 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2858 3615 4292 5440 5685 1606
Size: 1252 757 677 1148 245 2120
Fragments arranged by size:
2120 1252 1148 757 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4708
Fragments arranged by size:
4708 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6199
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6199
NotI GC'GGCC_GC
Cuts at: 3646 3646
Size: 6199
NspI r_CATG'y
Cuts at: 1132 1229 2316 2892 3557 5102 1132
Size: 97 1087 576 665 1545 2229
Fragments arranged by size:
2229 1545 1087 665 576 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6199
PciI A'CATG_T
Cuts at: 5098 5098
Size: 6199
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6199
PfoI T'CCnGG_A
Cuts at: 2119 2119
Size: 6199
PmeI GTTT'AAAC
Cuts at: 3503 3503
Size: 6199
PmlI CAC'GTG
Cuts at: 2067 2067
Size: 6199
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4268 4300 5781 5813 4268
Size: 32 1481 32 4654
Fragments arranged by size:
4654 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6199
PsiI TTA'TAA
Cuts at: 3769 3769
Size: 6199
PspOMI G'GGCC_C
Cuts at: 2320 2320
Size: 6199
PstI C_TGCA'G
Cuts at: 830 2637 3551 830
Size: 1807 914 3478
Fragments arranged by size:
3478 1807 914
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6199
SacII CC_GC'GG
Cuts at: 2859 2859
Size: 6199
SalI G'TCGA_C
Cuts at: 1936 3541 1936
Size: 1605 4594
SbfI CC_TGCA'GG
Cuts at: 3551 3551
Size: 6199
ScaI AGT'ACT
Cuts at: 3492 4447 6159 3492
Size: 955 1712 3532
Fragments arranged by size:
3532 1712 955
SfcI C'TryA_G
Cuts at: 826 1045 2633 3547 4682 5363 5554 826
Size: 219 1588 914 1135 681 191 1471
Fragments arranged by size:
1588 1471 1135 914 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6199
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6199
SmaI CCC'GGG
Cuts at: 1872 3143 3526 1872
Size: 1271 383 4545
Fragments arranged by size:
4545 1271 383
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2497 2516 2950 3404 4303 5204
Size: 197 926 554 19 434 454 899 901
Cuts at: 5204 5466 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 899 554 454 434 262
197 19
SnaBI TAC'GTA
Cuts at: 493 2551 3990 493
Size: 2058 1439 2702
Fragments arranged by size:
2702 2058 1439
SpeI A'CTAG_T
Cuts at: 152 3487 152
Size: 3335 2864
SphI G_CATG'C
Cuts at: 1132 2316 3557 1132
Size: 1184 1241 3774
Fragments arranged by size:
3774 1241 1184
SspI AAT'ATT
Cuts at: 5 52 4123 5
Size: 47 4071 2081
Fragments arranged by size:
4071 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6199
StyI C'CwwG_G
Cuts at: 513 1065 2004 3638 513
Size: 552 939 1634 3074
Fragments arranged by size:
3074 1634 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4226 4243 4396 4581 5026 325
Size: 1255 2646 17 153 185 445 1498
Fragments arranged by size:
2646 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2850 3444 3490
Size: 274 80 33 51 1443 873 594 46
Cuts at: 3490 4445 6157 96
Size: 955 1712 138
Fragments arranged by size:
1712 1443 955 873 594 274 138 80
51 46 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2580 2612 6159 6191 2580
Size: 32 3547 32 2588
Fragments arranged by size:
3547 2588 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6199
XbaI T'CTAG_A
Cuts at: 3535 3535
Size: 6199
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6199
XmaI C'CCGG_G
Cuts at: 1870 3141 3524 1870
Size: 1271 383 4545
Fragments arranged by size:
4545 1271 383
XmnI GAAnn'nnTTC
Cuts at: 4328 4328
Size: 6199
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4007 276
Size: 53 83 186 936 2473 2468
Fragments arranged by size:
2473 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI