Restriction map for
pFN21AE4480
(Circular) (Six-base) MAPSORT of: pFN21AE4480.seq Check: 5762 from: 1 to: 6196
pFN21AE4480 6196 bp
With 182 enzymes: SgfI *
March 28, 2012 13:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4006 278
Size: 53 83 186 936 2470 2468
Fragments arranged by size:
2470 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2823 3539 1937
Size: 886 716 4594
Fragments arranged by size:
4594 886 716
Acc65I G'GTAC_C
Cuts at: 3517 3517
Size: 6196
AclI AA'CG_TT
Cuts at: 1563 2590 4323 4696 1563
Size: 1027 1733 373 3063
Fragments arranged by size:
3063 1733 1027 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2372 3247 4259 5643 5911 1632
Size: 740 875 1012 1384 268 1917
Fragments arranged by size:
1917 1384 1012 875 740 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5999
AflIII A'CryG_T
Cuts at: 1203 5095 6072 1203
Size: 3892 977 1327
Fragments arranged by size:
3892 1327 977
AgeI A'CCGG_T
Cuts at: 5027 5027
Size: 6196
AhdI GACnn_n'nnGTC
Cuts at: 4925 4925
Size: 6196
AleI CACnn'nnGTG
Cuts at: 1166 2768 1166
Size: 1602 4594
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6196
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3160 3192 3160
Size: 32 6164
AlwNI CAG_nnn'CTG
Cuts at: 3939 5511 3939
Size: 1572 4624
ApaLI G'TGCA_C
Cuts at: 2941 4253 5409 2941
Size: 1312 1156 3728
Fragments arranged by size:
3728 1312 1156
ApoI r'AATT_y
Cuts at: 1493 3505 3731 3883 5992 1493
Size: 2012 226 152 2109 1697
Fragments arranged by size:
2109 2012 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4750 160
Size: 872 3718 1606
Fragments arranged by size:
3718 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6196
AvaI C'yCGr_G
Cuts at: 1870 1943 2151 3521 1870
Size: 73 208 1370 4545
Fragments arranged by size:
4545 1370 208 73
BamHI G'GATC_C
Cuts at: 2279 2395 3526 3893 2279
Size: 116 1131 367 4582
Fragments arranged by size:
4582 1131 367 116
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3517 4972 618
Size: 325 217 627 1730 1455 1842
Fragments arranged by size:
1842 1730 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3515 1391
Size: 2124 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2073 928
Size: 971 174 5051
Fragments arranged by size:
5051 971 174
BbvCI CC'TCA_GC
Cuts at: 2924 2924
Size: 6196
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4387 4421 1903
Size: 34 2450 34 3678
Fragments arranged by size:
3678 2450 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2575 4088 5298 2575
Size: 1513 1210 3473
Fragments arranged by size:
3473 1513 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6196
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4807 136
Size: 107 122 71 631 605 3135 1525
Fragments arranged by size:
3135 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3251 6191 3251
Size: 2940 3256
BlpI GC'TnA_GC
Cuts at: 2958 3114 3613 2958
Size: 156 499 5541
Fragments arranged by size:
5541 499 156
Bme1580I G_kGCm'C
Cuts at: 1790 2904 2945 4257 5413 1790
Size: 1114 41 1312 1156 2573
Fragments arranged by size:
2573 1312 1156 1114 41
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6196
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2355 3927 4885
Size: 416 53 83 399 24 938 1572 958
Cuts at: 4885 442
Size: 1753
Fragments arranged by size:
1753 1572 958 938 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6196
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2627 4856 1239
Size: 1388 2229 2579
Fragments arranged by size:
2579 2229 1388
Bpu10I CC'TnA_GC
Cuts at: 2924 3108 2924
Size: 184 6012
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4321 5186 5484 4321
Size: 865 298 5033
Fragments arranged by size:
5033 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3203 4859 882
Size: 617 1704 1656 2219
Fragments arranged by size:
2219 1704 1656 617
BsaAI yAC'GTr
Cuts at: 493 1204 3987 493
Size: 711 2783 2702
Fragments arranged by size:
2783 2702 711
BsaBI GATnn'nnATC
Cuts at: 6190 6190
Size: 6196
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4003 4385 5020 275
Size: 53 83 186 936 2470 382 635 1451
Fragments arranged by size:
2470 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4629 5027 5301 5448 5859 4629
Size: 398 274 147 411 4966
Fragments arranged by size:
4966 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2649 2679 3160 3190 1178
Size: 30 165 30 1246 30 481 30 4184
Fragments arranged by size:
4184 1246 481 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2480 2806 1186
Size: 1294 326 4576
Fragments arranged by size:
4576 1294 326
BseYI C'CCAG_C
Cuts at: 5399 5895 5399
Size: 496 5700
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5623
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3646 4407 5435 1540
Size: 275 186 1645 761 1028 2301
Fragments arranged by size:
2301 1645 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2945 4257 4342 5413 2945
Size: 1312 85 1071 3728
Fragments arranged by size:
3728 1312 1071 85
BsmI GAATG_Cn'
Cuts at: 2235 3707 3800 2235
Size: 1472 93 4631
Fragments arranged by size:
4631 1472 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2904 2945 3515 4257 4342 5413 1391
Size: 399 1114 41 570 742 85 1071 2174
Fragments arranged by size:
2174 1114 1071 742 570 399 85 41
BspEI T'CCGG_A
Cuts at: 5859 5859
Size: 6196
BspHI T'CATG_A
Cuts at: 2464 4085 5815 2464
Size: 1621 1730 2845
Fragments arranged by size:
2845 1730 1621
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2167 2788 3190 3551 844
Size: 1323 621 402 361 3489
Fragments arranged by size:
3489 1323 621 402 361
BsrBI CCG'CTC
Cuts at: 1394 1823 4083 1394
Size: 429 2260 3507
Fragments arranged by size:
3507 2260 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4691 4865 65
Size: 1183 3443 174 1396
Fragments arranged by size:
3443 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4840 5027 1700
Size: 110 3030 187 2869
Fragments arranged by size:
3030 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2406 2732 96
Size: 2310 326 3560
Fragments arranged by size:
3560 2310 326
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6196
BssSI C'ACGA_G
Cuts at: 4256 5268 4256
Size: 1012 5184
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6196
BstBI TT'CG_AA
Cuts at: 5000 5000
Size: 6196
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6196
BstYI r'GATC_y
Cuts at: 1972 2279 2395 2659 3251 3526 3893 4278 4295
Size: 307 116 264 592 275 367 385 17
Cuts at: 4295 5747 5833 5845 6191 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 592 385 367 346 307 275
264 116 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2789 2789
Size: 6196
BtgI C'CryG_G
Cuts at: 513 1065 2577 513
Size: 552 1512 4132
Fragments arranged by size:
4132 1512 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5457
Fragments arranged by size:
5457 683 56
BtsI GCAGTG_nn'
Cuts at: 3712 4506 4526 3712
Size: 794 20 5382
Fragments arranged by size:
5382 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6196
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6161
DraI TTT'AAA
Cuts at: 3500 3500
Size: 6196
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5203 809
Size: 507 3887 1802
Fragments arranged by size:
3887 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3643 4532 6161
Size: 54 1425 50 275 18 1813 889 1629
Cuts at: 6161 8
Size: 43
Fragments arranged by size:
1813 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3643 1537
Size: 275 1831 4090
Fragments arranged by size:
4090 1831 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4126 1353
Size: 2773 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4783 5157 5303 387
Size: 1064 138 3194 374 146 1280
Fragments arranged by size:
3194 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2372 2627 3247 4259 4856 5643 5911
Size: 393 740 255 620 1012 597 787 268
Cuts at: 5911 1239
Size: 1524
Fragments arranged by size:
1524 1012 787 740 620 597 393 268
255
EcoO109I rG'GnC_Cy
Cuts at: 2143 2143
Size: 6196
EcoRI G'AATT_C
Cuts at: 3505 3883 3505
Size: 378 5818
EcoRV GAT'ATC
Cuts at: 2701 2701
Size: 6196
FspI TGC'GCA
Cuts at: 4702 4702
Size: 6196
HaeII r_GCGC'y
Cuts at: 1678 5343 1678
Size: 3665 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2301 2333 2380 2412 3236
Size: 32 135 32 729 32 47 32 824
Cuts at: 3236 3268 4837 4869 4911 4943 1373
Size: 32 1569 32 42 32 2626
Fragments arranged by size:
2626 1569 824 729 135 47 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2257 2314 3337 3540 3786 669
Size: 988 281 319 57 1023 203 246 3079
Fragments arranged by size:
3079 1023 988 319 281 246 203 57
HindIII A'AGCT_T
Cuts at: 748 3277 748
Size: 2529 3667
HpaI GTT'AAC
Cuts at: 2314 3337 3786 2314
Size: 1023 449 4724
Fragments arranged by size:
4724 1023 449
KpnI G_GTAC'C
Cuts at: 3521 3521
Size: 6196
MfeI C'AATT_G
Cuts at: 3795 3795
Size: 6196
MluI A'CGCG_T
Cuts at: 6072 6072
Size: 6196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2423 3253 3428 5310 5494 1305
Size: 1118 830 175 1882 184 2007
Fragments arranged by size:
2007 1882 1118 830 184 175
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4374
Fragments arranged by size:
4374 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2768 4154 4513 4672 518
Size: 648 1602 1386 359 159 2042
Fragments arranged by size:
2042 1602 1386 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2957 3612 4289 5437 5682 1606
Size: 1351 655 677 1148 245 2120
Fragments arranged by size:
2120 1351 1148 677 655 245
NcoI C'CATG_G
Cuts at: 513 1065 2577 513
Size: 552 1512 4132
Fragments arranged by size:
4132 1512 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6196
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6196
NotI GC'GGCC_GC
Cuts at: 3643 3643
Size: 6196
NspI r_CATG'y
Cuts at: 1132 1229 2769 3554 5099 1132
Size: 97 1540 785 1545 2229
Fragments arranged by size:
2229 1545 1540 785 97
PasI CC'CwG_GG
Cuts at: 1348 2905 1348
Size: 1557 4639
PciI A'CATG_T
Cuts at: 5095 5095
Size: 6196
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6196
PmeI GTTT'AAAC
Cuts at: 3500 3500
Size: 6196
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3160 3192 4265 4297 5778 5810 3160
Size: 32 1073 32 1481 32 3546
Fragments arranged by size:
3546 1481 1073 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2143 2143
Size: 6196
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6196
PsiI TTA'TAA
Cuts at: 3766 3766
Size: 6196
PstI C_TGCA'G
Cuts at: 830 3548 830
Size: 2718 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6196
SalI G'TCGA_C
Cuts at: 1936 3538 1936
Size: 1602 4594
SbfI CC_TGCA'GG
Cuts at: 3548 3548
Size: 6196
ScaI AGT'ACT
Cuts at: 4444 6156 4444
Size: 1712 4484
SfcI C'TryA_G
Cuts at: 826 1045 2305 3544 4679 5360 5551 826
Size: 219 1260 1239 1135 681 191 1471
Fragments arranged by size:
1471 1260 1239 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6196
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6196
SmaI CCC'GGG
Cuts at: 1872 3523 1872
Size: 1651 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4300 5201 5463 820
Size: 197 926 2357 901 262 1553
Fragments arranged by size:
2357 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3987 493
Size: 3494 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6196
SphI G_CATG'C
Cuts at: 1132 3554 1132
Size: 2422 3774
SspI AAT'ATT
Cuts at: 5 52 4120 5
Size: 47 4068 2081
Fragments arranged by size:
4068 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6196
StyI C'CwwG_G
Cuts at: 513 1065 2577 3635 513
Size: 552 1512 1058 3074
Fragments arranged by size:
3074 1512 1058 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2041 4223 4240 4393 4578 5023 325
Size: 1255 461 2182 17 153 185 445 1498
Fragments arranged by size:
2182 1498 1255 461 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2406 2732 3230
Size: 274 80 33 51 1443 429 326 498
Cuts at: 3230 4442 6154 96
Size: 1212 1712 138
Fragments arranged by size:
1712 1443 1212 498 429 326 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2649 2681 6156 6188 2649
Size: 32 3475 32 2657
Fragments arranged by size:
3475 2657 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6196
XbaI T'CTAG_A
Cuts at: 3532 3532
Size: 6196
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2902 2902
Size: 6196
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6196
XmaI C'CCGG_G
Cuts at: 1870 3521 1870
Size: 1651 4545
XmnI GAAnn'nnTTC
Cuts at: 4325 4325
Size: 6196
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4004 276
Size: 53 83 186 936 2470 2468
Fragments arranged by size:
2470 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI