Restriction map for
pFN21AE4478
(Circular) (Six-base) MAPSORT of: pFN21AE4478.seq Check: 9504 from: 1 to: 6193
pFN21AE4478 6193 bp
With 182 enzymes: SgfI *
March 28, 2012 14:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2891 2891
Size: 6193
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4003 278
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3536 1937
Size: 1599 4594
Acc65I G'GTAC_C
Cuts at: 3514 3514
Size: 6193
AclI AA'CG_TT
Cuts at: 1563 4320 4693 1563
Size: 2757 373 3063
Fragments arranged by size:
3063 2757 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2565 3166 4256 5640 5908 1632
Size: 933 601 1090 1384 268 1917
Fragments arranged by size:
1917 1384 1090 933 601 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5996
AflIII A'CryG_T
Cuts at: 1203 5092 6069 1203
Size: 3889 977 1327
Fragments arranged by size:
3889 1327 977
AgeI A'CCGG_T
Cuts at: 5024 5024
Size: 6193
AhdI GACnn_n'nnGTC
Cuts at: 2255 4922 2255
Size: 2667 3526
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6193
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2300 1184
Size: 1116 5077
AlwNI CAG_nnn'CTG
Cuts at: 3936 5508 3936
Size: 1572 4621
ApaI G_GGCC'C
Cuts at: 3194 3194
Size: 6193
ApaLI G'TGCA_C
Cuts at: 4250 5406 4250
Size: 1156 5037
ApoI r'AATT_y
Cuts at: 1493 2123 2499 2528 3502 3728 3880 5989 1493
Size: 630 376 29 974 226 152 2109 1697
Fragments arranged by size:
2109 1697 974 630 376 226 152 29
AseI AT'TA_AT
Cuts at: 160 1032 4747 160
Size: 872 3715 1606
Fragments arranged by size:
3715 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
AvaI C'yCGr_G
Cuts at: 1870 1943 3518 1870
Size: 73 1575 4545
Fragments arranged by size:
4545 1575 73
BamHI G'GATC_C
Cuts at: 3523 3890 3523
Size: 367 5826
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2459 2696 3514 4969 618
Size: 325 217 627 672 237 818 1455 1842
Fragments arranged by size:
1842 1455 818 672 627 325 237 217
BanII G_rGCy'C
Cuts at: 1391 2784 2916 3194 3512 1391
Size: 1393 132 278 318 4072
Fragments arranged by size:
4072 1393 318 278 132
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5222
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4384 4418 1903
Size: 34 2447 34 3678
Fragments arranged by size:
3678 2447 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2719 4085 5295 2719
Size: 1366 1210 3617
Fragments arranged by size:
3617 1366 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6193
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4804 136
Size: 107 122 71 631 605 3132 1525
Fragments arranged by size:
3132 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2436 2514 3425 6188 2436
Size: 78 911 2763 2441
Fragments arranged by size:
2763 2441 911 78
BlpI GC'TnA_GC
Cuts at: 2670 3610 2670
Size: 940 5253
Bme1580I G_kGCm'C
Cuts at: 1790 2421 2699 3194 4254 5410 1790
Size: 631 278 495 1060 1156 2573
Fragments arranged by size:
2573 1156 1060 631 495 278
BmgBI CAC'GTC
Cuts at: 1318 2184 1318
Size: 866 5327
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2097 2576 3924
Size: 416 53 83 399 24 680 479 1348
Cuts at: 3924 4882 442
Size: 958 1753
Fragments arranged by size:
1753 1348 958 680 479 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2666 2816 4853 1239
Size: 1427 150 2037 2579
Fragments arranged by size:
2579 2037 1427 150
Bpu10I CC'TnA_GC
Cuts at: 3471 3471
Size: 6193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4318 5183 5481 4318
Size: 865 298 5030
Fragments arranged by size:
5030 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3353 3470 4856 882
Size: 617 1854 117 1386 2219
Fragments arranged by size:
2219 1854 1386 617 117
BsaAI yAC'GTr
Cuts at: 493 1204 3984 493
Size: 711 2780 2702
Fragments arranged by size:
2780 2702 711
BsaBI GATnn'nnATC
Cuts at: 6187 6187
Size: 6193
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3441 4000 4382 5017
Size: 53 83 186 936 1908 559 382 635
Cuts at: 5017 275
Size: 1451
Fragments arranged by size:
1908 1451 936 635 559 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4626 5024 5298 5445 5856 4626
Size: 398 274 147 411 4963
Fragments arranged by size:
4963 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3221 3251 3279 3309 1178
Size: 30 165 30 1818 30 28 30 4062
Fragments arranged by size:
4062 1818 165 30 30 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2675 1186
Size: 1489 4704
BseYI C'CCAG_C
Cuts at: 2026 2482 3199 5396 5892 2026
Size: 456 717 2197 496 2327
Fragments arranged by size:
2327 2197 717 496 456
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2389 2622 3438 1163
Size: 573 653 233 816 3918
Fragments arranged by size:
3918 816 653 573 233
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3643 4404 5432 1540
Size: 275 186 1642 761 1028 2301
Fragments arranged by size:
2301 1642 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2784 2832 4254 4339 5410 2784
Size: 48 1422 85 1071 3567
Fragments arranged by size:
3567 1422 1071 85 48
BsmI GAATG_Cn'
Cuts at: 2671 3704 3797 2671
Size: 1033 93 5067
Fragments arranged by size:
5067 1033 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2190 2190
Size: 6193
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2421 2699 2784 2832 2916 3194 3512
Size: 399 631 278 85 48 84 278 318
Cuts at: 3512 4254 4339 5410 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 631 399 318 278 278
85 85 84 48
BspEI T'CCGG_A
Cuts at: 5856 5856
Size: 6193
BspHI T'CATG_A
Cuts at: 3048 4082 5812 3048
Size: 1034 1730 3429
Fragments arranged by size:
3429 1730 1034
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2891 3548 844
Size: 2047 657 3489
Fragments arranged by size:
3489 2047 657
BsrBI CCG'CTC
Cuts at: 1394 1823 4080 1394
Size: 429 2257 3507
Fragments arranged by size:
3507 2257 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4688 4862 65
Size: 1183 3440 174 1396
Fragments arranged by size:
3440 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3218 3311 4837 5024 1700
Size: 110 1408 93 1526 187 2869
Fragments arranged by size:
2869 1526 1408 187 110 93
BsrGI T'GTAC_A
Cuts at: 96 2424 96
Size: 2328 3865
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6193
BssSI C'ACGA_G
Cuts at: 4253 5265 4253
Size: 1012 5181
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3416 794
Size: 2622 3571
BstBI TT'CG_AA
Cuts at: 4997 4997
Size: 6193
BstEII G'GTnAC_C
Cuts at: 1184 2020 1184
Size: 836 5357
BstXI CCAn_nnnn'nTGG
Cuts at: 2519 2519
Size: 6193
BstYI r'GATC_y
Cuts at: 1972 2436 2514 2676 3425 3523 3890 4275 4292
Size: 464 78 162 749 98 367 385 17
Cuts at: 4292 5744 5830 5842 6188 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 749 464 385 367 346 162
98 86 78 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3364 513
Size: 552 939 1360 3342
Fragments arranged by size:
3342 1360 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3221 487
Size: 683 56 1995 3459
Fragments arranged by size:
3459 1995 683 56
BtsI GCAGTG_nn'
Cuts at: 3219 3709 4503 4523 3219
Size: 490 794 20 4889
Fragments arranged by size:
4889 794 490 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6193
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6158
DraI TTT'AAA
Cuts at: 3497 3497
Size: 6193
DraIII CAC_nnn'GTG
Cuts at: 3416 3416
Size: 6193
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5200 809
Size: 507 3884 1802
Fragments arranged by size:
3884 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2205 2555 3171
Size: 54 1425 50 275 18 375 350 616
Cuts at: 3171 3216 3640 4529 6158 8
Size: 45 424 889 1629 43
Fragments arranged by size:
1629 1425 889 616 424 375 350 275
54 50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3640 1537
Size: 275 1828 4090
Fragments arranged by size:
4090 1828 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2258 2396 2541 2652 3185 3266 4123 1353
Size: 905 138 145 111 533 81 857 3423
Fragments arranged by size:
3423 905 857 533 145 138 111 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4780 5154 5300 387
Size: 1064 138 3191 374 146 1280
Fragments arranged by size:
3191 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2782 2782
Size: 6193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2565 2666 2816 3166 4256 4853 5640
Size: 393 933 101 150 350 1090 597 787
Cuts at: 5640 5908 1239
Size: 268 1524
Fragments arranged by size:
1524 1090 933 787 597 393 350 268
150 101
EcoO109I rG'GnC_Cy
Cuts at: 3149 3190 3191 3149
Size: 41 1 6151
Fragments arranged by size:
6151 41 1
EcoRI G'AATT_C
Cuts at: 2499 2528 3502 3880 2499
Size: 29 974 378 4812
Fragments arranged by size:
4812 974 378 29
EcoRV GAT'ATC
Cuts at: 3385 3385
Size: 6193
FspI TGC'GCA
Cuts at: 4699 4699
Size: 6193
HaeII r_GCGC'y
Cuts at: 1678 5340 1678
Size: 3662 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2241 2273 2356 2388 2506
Size: 32 135 32 669 32 83 32 118
Cuts at: 2506 2538 2625 2657 2796 2828 4834 4866 4908
Size: 32 87 32 139 32 2006 32 42
Cuts at: 4908 4940 1373
Size: 32 2626
Fragments arranged by size:
2626 2006 669 139 135 118 87 83
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2260 2296 3537 3783 669
Size: 988 281 322 36 1241 246 3079
Fragments arranged by size:
3079 1241 988 322 281 246 36
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6193
HpaI GTT'AAC
Cuts at: 3783 3783
Size: 6193
KpnI G_GTAC'C
Cuts at: 3518 3518
Size: 6193
MfeI C'AATT_G
Cuts at: 3792 3792
Size: 6193
MluI A'CGCG_T
Cuts at: 6069 6069
Size: 6193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2227 2476 3232 3252 3264 5307 5491 1305
Size: 922 249 756 20 12 2043 184 2007
Fragments arranged by size:
2043 2007 922 756 249 184 20 12
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2207 10
Size: 54 1425 343 375 3996
Fragments arranged by size:
3996 1425 375 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3047 3449 4151 4510 4669 518
Size: 648 1881 402 702 359 159 2042
Fragments arranged by size:
2042 1881 702 648 402 359 159
MspA1I CmG'CkG
Cuts at: 1606 2482 2560 2686 3203 3272 3609 4286 5434
Size: 876 78 126 517 69 337 677 1148
Cuts at: 5434 5679 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 876 677 517 337 245 126
78 69
NaeI GCC'GGC
Cuts at: 3220 3220
Size: 6193
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4702
Fragments arranged by size:
4702 939 552
NdeI CA'TA_TG
Cuts at: 387 3009 387
Size: 2622 3571
NgoMIV G'CCGG_C
Cuts at: 3218 3218
Size: 6193
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6193
NotI GC'GGCC_GC
Cuts at: 3640 3640
Size: 6193
NspI r_CATG'y
Cuts at: 1132 1229 3551 5096 1132
Size: 97 2322 1545 2229
Fragments arranged by size:
2322 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6193
PciI A'CATG_T
Cuts at: 5092 5092
Size: 6193
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3159 1108
Size: 2051 4142
PfoI T'CCnGG_A
Cuts at: 2721 2721
Size: 6193
PmeI GTTT'AAAC
Cuts at: 3497 3497
Size: 6193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4262 4294 5775 5807 4262
Size: 32 1481 32 4648
Fragments arranged by size:
4648 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6193
PsiI TTA'TAA
Cuts at: 3763 3763
Size: 6193
PspOMI G'GGCC_C
Cuts at: 3190 3190
Size: 6193
PstI C_TGCA'G
Cuts at: 830 3545 830
Size: 2715 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6193
PvuII CAG'CTG
Cuts at: 2482 3203 2482
Size: 721 5472
SacI G_AGCT'C
Cuts at: 2784 2784
Size: 6193
SalI G'TCGA_C
Cuts at: 1936 3535 1936
Size: 1599 4594
SapI GCTCTTCn'nnn_
Cuts at: 2541 3266 2541
Size: 725 5468
SbfI CC_TGCA'GG
Cuts at: 3545 3545
Size: 6193
ScaI AGT'ACT
Cuts at: 4441 6153 4441
Size: 1712 4481
SfcI C'TryA_G
Cuts at: 826 1045 2961 3541 4676 5357 5548 826
Size: 219 1916 580 1135 681 191 1471
Fragments arranged by size:
1916 1471 1135 681 580 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6193
SmaI CCC'GGG
Cuts at: 1872 3520 1872
Size: 1648 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4297 5198 5460 820
Size: 197 926 2354 901 262 1553
Fragments arranged by size:
2354 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3984 493
Size: 3491 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6193
SphI G_CATG'C
Cuts at: 1132 3551 1132
Size: 2419 3774
SspI AAT'ATT
Cuts at: 5 52 4117 5
Size: 47 4065 2081
Fragments arranged by size:
4065 2081 47
StuI AGG'CCT
Cuts at: 1517 3018 1517
Size: 1501 4692
StyI C'CwwG_G
Cuts at: 513 1065 2004 2536 3019 3632 513
Size: 552 939 532 483 613 3074
Fragments arranged by size:
3074 939 613 552 532 483
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2714 4220 4237 4390 4575 5020 325
Size: 1255 1134 1506 17 153 185 445 1498
Fragments arranged by size:
1506 1498 1255 1134 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2424 4439 6151
Size: 274 80 33 51 1443 447 2015 1712
Cuts at: 6151 96
Size: 138
Fragments arranged by size:
2015 1712 1443 447 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2490 2522 6153 6185 2490
Size: 32 3631 32 2498
Fragments arranged by size:
3631 2498 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3440 1316
Size: 2124 4069
XbaI T'CTAG_A
Cuts at: 3529 3529
Size: 6193
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6193
XmaI C'CCGG_G
Cuts at: 1870 3518 1870
Size: 1648 4545
XmnI GAAnn'nnTTC
Cuts at: 4322 4322
Size: 6193
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4001 276
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BstZ17I Bsu36I EcoNI FalI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PpuMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI