Restriction map for
pFN21AE4477
(Circular) (Six-base) MAPSORT of: pFN21AE4477.seq Check: 6841 from: 1 to: 6190
pFN21AE4477 6190 bp
With 182 enzymes: SgfI *
March 28, 2012 14:04 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4000 278
Size: 53 83 186 936 2464 2468
Fragments arranged by size:
2468 2464 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2205 3533 1937
Size: 268 1328 4594
Fragments arranged by size:
4594 1328 268
Acc65I G'GTAC_C
Cuts at: 3511 3511
Size: 6190
AclI AA'CG_TT
Cuts at: 1563 4317 4690 1563
Size: 2754 373 3063
Fragments arranged by size:
3063 2754 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4253 5637 5905 1632
Size: 2621 1384 268 1917
Fragments arranged by size:
2621 1917 1384 268
AfeI AGC'GCT
Cuts at: 2939 2939
Size: 6190
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5993
AflIII A'CryG_T
Cuts at: 1203 3294 5089 6066 1203
Size: 2091 1795 977 1327
Fragments arranged by size:
2091 1795 1327 977
AgeI A'CCGG_T
Cuts at: 5021 5021
Size: 6190
AhdI GACnn_n'nnGTC
Cuts at: 4919 4919
Size: 6190
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6190
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3257 1184
Size: 2073 4117
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3404 3436 3404
Size: 32 6158
AlwNI CAG_nnn'CTG
Cuts at: 2710 3349 3933 5505 2710
Size: 639 584 1572 3395
Fragments arranged by size:
3395 1572 639 584
ApaI G_GGCC'C
Cuts at: 2113 2121 3429 2113
Size: 8 1308 4874
Fragments arranged by size:
4874 1308 8
ApaLI G'TGCA_C
Cuts at: 4247 5403 4247
Size: 1156 5034
ApoI r'AATT_y
Cuts at: 1493 2101 3499 3725 3877 5986 1493
Size: 608 1398 226 152 2109 1697
Fragments arranged by size:
2109 1697 1398 608 226 152
AscI GG'CGCG_CC
Cuts at: 2732 2732
Size: 6190
AseI AT'TA_AT
Cuts at: 160 1032 4744 160
Size: 872 3712 1606
Fragments arranged by size:
3712 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6190
AvaI C'yCGr_G
Cuts at: 1870 1943 2106 2112 2195 3029 3329 3428 3515
Size: 73 163 6 83 834 300 99 87
Cuts at: 3515 1870
Size: 4545
Fragments arranged by size:
4545 834 300 163 99 87 83 73
6
AvrII C'CTAG_G
Cuts at: 2096 2096
Size: 6190
BamHI G'GATC_C
Cuts at: 3520 3887 3520
Size: 367 5823
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2191 2413 2505 2638 2660
Size: 325 217 627 404 222 92 133 22
Cuts at: 2660 3352 3385 3511 4966 618
Size: 692 33 126 1455 1842
Fragments arranged by size:
1842 1455 692 627 404 325 222 217
133 126 92 33 22
BanII G_rGCy'C
Cuts at: 1391 2113 2121 3417 3429 3509 1391
Size: 722 8 1296 12 80 4072
Fragments arranged by size:
4072 1296 722 80 12 8
BbeI G_GCGC'C
Cuts at: 2642 3389 2642
Size: 747 5443
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5219
BbvCI CC'TCA_GC
Cuts at: 2409 2409
Size: 6190
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4381 4415 1903
Size: 34 50 34 2360 34 3678
Fragments arranged by size:
3678 2360 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4082 5292 4082
Size: 1210 4980
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6190
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2036 4801 136
Size: 107 122 71 631 605 364 2765 1525
Fragments arranged by size:
2765 1525 631 605 364 122 107 71
BglII A'GATC_T
Cuts at: 6185 6185
Size: 6190
BlpI GC'TnA_GC
Cuts at: 2775 3607 2775
Size: 832 5358
Bme1580I G_kGCm'C
Cuts at: 1790 2113 2121 2196 2663 3357 3429 4251 5407
Size: 323 8 75 467 694 72 822 1156
Cuts at: 5407 1790
Size: 2573
Fragments arranged by size:
2573 1156 822 694 467 323 75 72
8
BmgBI CAC'GTC
Cuts at: 1318 2465 3091 3118 1318
Size: 1147 626 27 4390
Fragments arranged by size:
4390 1147 626 27
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3442 3921 4879
Size: 416 53 83 399 24 2025 479 958
Cuts at: 4879 442
Size: 1753
Fragments arranged by size:
2025 1753 958 479 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6190
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3055 3172 3055
Size: 117 6073
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 2447 2876 3023 3431 4850 1239
Size: 1061 147 429 147 408 1419 2579
Fragments arranged by size:
2579 1419 1061 429 408 147 147
Bpu10I CC'TnA_GC
Cuts at: 2409 2409
Size: 6190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3401 4315 5180 5478 3401
Size: 914 865 298 4113
Fragments arranged by size:
4113 914 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2351 2873 2969 4853 882
Size: 617 852 522 96 1884 2219
Fragments arranged by size:
2219 1884 852 617 522 96
BsaAI yAC'GTr
Cuts at: 493 1204 2365 3247 3297 3981 493
Size: 711 1161 882 50 684 2702
Fragments arranged by size:
2702 1161 882 711 684 50
BsaBI GATnn'nnATC
Cuts at: 6184 6184
Size: 6190
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2580 2639 2945 3279
Size: 53 83 186 936 1047 59 306 334
Cuts at: 3279 3386 3997 4379 5014 275
Size: 107 611 382 635 1451
Fragments arranged by size:
1451 1047 936 635 611 382 334 306
186 107 83 59 53
BsaWI w'CCGG_w
Cuts at: 4623 5021 5295 5442 5853 4623
Size: 398 274 147 411 4960
Fragments arranged by size:
4960 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2583 2613 3404 3434 1178
Size: 30 165 30 1180 30 791 30 3934
Fragments arranged by size:
3934 1180 791 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2948 1186
Size: 1762 4428
BseYI C'CCAG_C
Cuts at: 2227 2232 2694 2818 2838 3046 5393 5889 2227
Size: 5 462 124 20 208 2347 496 2528
Fragments arranged by size:
2528 2347 496 462 208 124 20 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2723 3239 1163
Size: 573 987 516 4114
Fragments arranged by size:
4114 987 573 516
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2040 2654 3198 3640 4401 5429
Size: 275 186 39 614 544 442 761 1028
Cuts at: 5429 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 614 544 442 275 186
39
BsiHKAI G_wGCw'C
Cuts at: 2607 3336 3417 4251 4336 5407 2607
Size: 729 81 834 85 1071 3390
Fragments arranged by size:
3390 1071 834 729 85 81
BsmI GAATG_Cn'
Cuts at: 3701 3794 3701
Size: 93 6097
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2113 2121 2196 2607 2663 3336 3357
Size: 399 323 8 75 411 56 673 21
Cuts at: 3357 3417 3429 3509 4251 4336 5407 1391
Size: 60 12 80 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 673 411 399 323 85
80 75 60 56 21 12 8
BspEI T'CCGG_A
Cuts at: 5853 5853
Size: 6190
BspHI T'CATG_A
Cuts at: 3282 4079 5809 3282
Size: 797 1730 3663
Fragments arranged by size:
3663 1730 797
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3545 844
Size: 2701 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4077 1394
Size: 429 2254 3507
Fragments arranged by size:
3507 2254 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3280 4685 4859 65
Size: 1183 2032 1405 174 1396
Fragments arranged by size:
2032 1405 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2138 2331 2653 3152 3309 3368 4834
Size: 110 328 193 322 499 157 59 1466
Cuts at: 4834 5021 1700
Size: 187 2869
Fragments arranged by size:
2869 1466 499 328 322 193 187 157
110 59
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6190
BssHII G'CGCG_C
Cuts at: 1925 2611 2732 2762 2814 2962 2964 1925
Size: 686 121 30 52 148 2 5151
Fragments arranged by size:
5151 686 148 121 52 30 2
BssSI C'ACGA_G
Cuts at: 2077 4250 5262 2077
Size: 2173 1012 3005
Fragments arranged by size:
3005 2173 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3340 794
Size: 2546 3644
BstBI TT'CG_AA
Cuts at: 4994 4994
Size: 6190
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6190
BstXI CCAn_nnnn'nTGG
Cuts at: 2509 2509
Size: 6190
BstYI r'GATC_y
Cuts at: 1972 3520 3887 4272 4289 5741 5827 5839 6185
Size: 1548 367 385 17 1452 86 12 346
Cuts at: 6185 1972
Size: 1977
Fragments arranged by size:
1977 1548 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2238 2487 2727 2751 2901 3061 3077
Size: 552 1173 249 240 24 150 160 16
Cuts at: 3077 513
Size: 3626
Fragments arranged by size:
3626 1173 552 249 240 160 150 24
16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5451
Fragments arranged by size:
5451 683 56
BtsI GCAGTG_nn'
Cuts at: 3041 3464 3706 4500 4520 3041
Size: 423 242 794 20 4711
Fragments arranged by size:
4711 794 423 242 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6190
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2359 2394 529
Size: 35 1795 35 4325
Fragments arranged by size:
4325 1795 35 35
DraI TTT'AAA
Cuts at: 3494 3494
Size: 6190
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5197 809
Size: 507 3881 1802
Fragments arranged by size:
3881 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2333 2367
Size: 54 1425 50 275 18 207 296 34
Cuts at: 2367 2472 2490 2651 2748 3637 4526 6155 8
Size: 105 18 161 97 889 889 1629 43
Fragments arranged by size:
1629 1425 889 889 296 275 207 161
105 97 54 50 43 34 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2037 2651 3637 1537
Size: 275 225 614 986 4090
Fragments arranged by size:
4090 986 614 275 225
EarI CTCTTCn'nnn_
Cuts at: 1353 2072 2630 4120 1353
Size: 719 558 1490 3423
Fragments arranged by size:
3423 1490 719 558
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2816 4777 5151 5297 387
Size: 1064 138 1227 1961 374 146 1280
Fragments arranged by size:
1961 1280 1227 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3415 3415
Size: 6190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2300 2447 2876 3023 3431 4253 4850
Size: 393 668 147 429 147 408 822 597
Cuts at: 4850 5637 5905 1239
Size: 787 268 1524
Fragments arranged by size:
1524 822 787 668 597 429 408 393
268 147 147
EcoNI CCTnn'n_nnAGG
Cuts at: 2930 2930
Size: 6190
EcoO109I rG'GnC_Cy
Cuts at: 2117 2118 2117
Size: 1 6189
EcoRI G'AATT_C
Cuts at: 2101 3499 3877 2101
Size: 1398 378 4414
Fragments arranged by size:
4414 1398 378
FspI TGC'GCA
Cuts at: 4696 4696
Size: 6190
HaeII r_GCGC'y
Cuts at: 1678 2312 2447 2642 2807 2941 3389 5337 1678
Size: 634 135 195 165 134 448 1948 2531
Fragments arranged by size:
2531 1948 634 448 195 165 135 134
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4831 4863 4905 4937 1373
Size: 32 135 32 3259 32 42 32 2626
Fragments arranged by size:
3259 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3534 3780 669
Size: 988 281 1596 246 3079
Fragments arranged by size:
3079 1596 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6190
HpaI GTT'AAC
Cuts at: 3780 3780
Size: 6190
KasI G'GCGC_C
Cuts at: 2638 3385 2638
Size: 747 5443
KpnI G_GTAC'C
Cuts at: 3515 3515
Size: 6190
MfeI C'AATT_G
Cuts at: 3789 3789
Size: 6190
MluI A'CGCG_T
Cuts at: 6066 6066
Size: 6190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5304 5488 1305
Size: 3999 184 2007
Fragments arranged by size:
3999 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2369 2750 10
Size: 54 1425 343 537 381 3450
Fragments arranged by size:
3450 1425 537 381 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4148 4507 4666 518
Size: 648 2982 359 159 2042
Fragments arranged by size:
2982 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2019 2574 2707 2729 2746 3136 3349 3606
Size: 413 555 133 22 17 390 213 257
Cuts at: 3606 4283 5431 5676 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 555 413 390 257 245
213 133 22 17
NaeI GCC'GGC
Cuts at: 2140 3154 3370 2140
Size: 1014 216 4960
Fragments arranged by size:
4960 1014 216
NarI GG'CG_CC
Cuts at: 2639 3386 2639
Size: 747 5443
NcoI C'CATG_G
Cuts at: 513 1065 3077 513
Size: 552 2012 3626
Fragments arranged by size:
3626 2012 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6190
NgoMIV G'CCGG_C
Cuts at: 2138 3152 3368 2138
Size: 1014 216 4960
Fragments arranged by size:
4960 1014 216
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6190
NotI GC'GGCC_GC
Cuts at: 2037 3637 2037
Size: 1600 4590
NspI r_CATG'y
Cuts at: 1132 1229 3548 5093 1132
Size: 97 2319 1545 2229
Fragments arranged by size:
2319 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6190
PciI A'CATG_T
Cuts at: 5089 5089
Size: 6190
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2239 1108
Size: 1131 5059
PfoI T'CCnGG_A
Cuts at: 3408 3408
Size: 6190
PmeI GTTT'AAAC
Cuts at: 3494 3494
Size: 6190
PmlI CAC'GTG
Cuts at: 3247 3297 3247
Size: 50 6140
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3404 3436 4259 4291 5772 5804 3404
Size: 32 823 32 1481 32 3790
Fragments arranged by size:
3790 1481 823 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6190
PsiI TTA'TAA
Cuts at: 3760 3760
Size: 6190
PspOMI G'GGCC_C
Cuts at: 2109 2117 3425 2109
Size: 8 1308 4874
Fragments arranged by size:
4874 1308 8
PspXI vC'TCGA_Gb
Cuts at: 3029 3029
Size: 6190
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3434 3466 3434
Size: 32 6158
PstI C_TGCA'G
Cuts at: 830 2088 2220 2679 3345 3542 830
Size: 1258 132 459 666 197 3478
Fragments arranged by size:
3478 1258 666 459 197 132
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6190
PvuII CAG'CTG
Cuts at: 2707 3136 2707
Size: 429 5761
SacI G_AGCT'C
Cuts at: 3417 3417
Size: 6190
SacII CC_GC'GG
Cuts at: 2730 2730
Size: 6190
SalI G'TCGA_C
Cuts at: 1936 3532 1936
Size: 1596 4594
SbfI CC_TGCA'GG
Cuts at: 3542 3542
Size: 6190
ScaI AGT'ACT
Cuts at: 4438 6150 4438
Size: 1712 4478
SfcI C'TryA_G
Cuts at: 826 1045 2084 2216 2675 3341 3538 4673 5354
Size: 219 1039 132 459 666 197 1135 681
Cuts at: 5354 5545 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1039 681 666 459 219 197
191 132
SfoI GGC'GCC
Cuts at: 2640 3387 2640
Size: 747 5443
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6190
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6190
SmaI CCC'GGG
Cuts at: 1872 2108 2114 3430 3517 1872
Size: 236 6 1316 87 4545
Fragments arranged by size:
4545 1316 236 87 6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3029 3416 4294 5195 5457 820
Size: 197 926 1086 387 878 901 262 1553
Fragments arranged by size:
1553 1086 926 901 878 387 262 197
SnaBI TAC'GTA
Cuts at: 493 3981 493
Size: 3488 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6190
SphI G_CATG'C
Cuts at: 1132 3548 1132
Size: 2416 3774
SspI AAT'ATT
Cuts at: 5 52 4114 5
Size: 47 4062 2081
Fragments arranged by size:
4062 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6190
StyI C'CwwG_G
Cuts at: 513 1065 2096 3077 3288 3629 513
Size: 552 1031 981 211 341 3074
Fragments arranged by size:
3074 1031 981 552 341 211
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4217 4234 4387 4572 5017 325
Size: 1255 2637 17 153 185 445 1498
Fragments arranged by size:
2637 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3222 4436 6148
Size: 274 80 33 51 1443 1245 1214 1712
Cuts at: 6148 96
Size: 138
Fragments arranged by size:
1712 1443 1245 1214 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2583 2615 2889 2921 6150 6182 2583
Size: 32 274 32 3229 32 2591
Fragments arranged by size:
3229 2591 274 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6190
XbaI T'CTAG_A
Cuts at: 3526 3526
Size: 6190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2302 2377 2302
Size: 75 6115
XhoI C'TCGA_G
Cuts at: 1943 3029 1943
Size: 1086 5104
XmaI C'CCGG_G
Cuts at: 1870 2106 2112 3428 3515 1870
Size: 236 6 1316 87 4545
Fragments arranged by size:
4545 1316 236 87 6
XmnI GAAnn'nnTTC
Cuts at: 4319 4319
Size: 6190
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3998 276
Size: 53 83 186 936 2464 2468
Fragments arranged by size:
2468 2464 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoRV FalI FseI FspAI NruI NsiI PacI PpuMI
RsrII SanDI SapI SexAI SfiI SrfI SwaI