Restriction map for
pFN21AE4473
(Circular) (Six-base) MAPSORT of: pFN21AE4473.seq Check: 6024 from: 1 to: 6184
pFN21AE4473 6184 bp
With 182 enzymes: SgfI *
March 28, 2012 13:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2738 2879 3219 2738
Size: 141 340 5703
Fragments arranged by size:
5703 340 141
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2382 2417 3369 3994
Size: 53 83 186 936 846 35 952 625
Cuts at: 3994 278
Size: 2468
Fragments arranged by size:
2468 952 936 846 625 186 83 53
35
AccI GT'mk_AC
Cuts at: 1937 2282 3527 1937
Size: 345 1245 4594
Fragments arranged by size:
4594 1245 345
Acc65I G'GTAC_C
Cuts at: 3505 3505
Size: 6184
AclI AA'CG_TT
Cuts at: 1563 4311 4684 1563
Size: 2748 373 3063
Fragments arranged by size:
3063 2748 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2072 4247 5631 5899 1632
Size: 440 2175 1384 268 1917
Fragments arranged by size:
2175 1917 1384 440 268
AfeI AGC'GCT
Cuts at: 2171 3329 2171
Size: 1158 5026
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5987
AflIII A'CryG_T
Cuts at: 1203 5083 6060 1203
Size: 3880 977 1327
Fragments arranged by size:
3880 1327 977
AgeI A'CCGG_T
Cuts at: 5015 5015
Size: 6184
AhdI GACnn_n'nnGTC
Cuts at: 4913 4913
Size: 6184
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6184
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3146 3410 1184
Size: 1962 264 3958
Fragments arranged by size:
3958 1962 264
AlwNI CAG_nnn'CTG
Cuts at: 2092 2475 3927 5499 2092
Size: 383 1452 1572 2777
Fragments arranged by size:
2777 1572 1452 383
ApaLI G'TGCA_C
Cuts at: 3131 4241 5397 3131
Size: 1110 1156 3918
Fragments arranged by size:
3918 1156 1110
ApoI r'AATT_y
Cuts at: 1493 3493 3719 3871 5980 1493
Size: 2000 226 152 2109 1697
Fragments arranged by size:
2109 2000 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4738 160
Size: 872 3706 1606
Fragments arranged by size:
3706 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
AvaI C'yCGr_G
Cuts at: 1870 1943 2700 3509 1870
Size: 73 757 809 4545
Fragments arranged by size:
4545 809 757 73
BamHI G'GATC_C
Cuts at: 3514 3881 3514
Size: 367 5817
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2056 2957 3207 3505 4960
Size: 325 217 627 269 901 250 298 1455
Cuts at: 4960 618
Size: 1842
Fragments arranged by size:
1842 1455 901 627 325 298 269 250
217
BanII G_rGCy'C
Cuts at: 1391 2788 2942 3180 3503 1391
Size: 1397 154 238 323 4072
Fragments arranged by size:
4072 1397 323 238 154
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5213
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3314 3348 4375 4409 1903
Size: 34 1377 34 1027 34 3678
Fragments arranged by size:
3678 1377 1027 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4076 5286 4076
Size: 1210 4974
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6184
BfrBI ATG'CAT
Cuts at: 3438 3438
Size: 6184
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3103 4795
Size: 107 122 71 631 605 337 1094 1692
Cuts at: 4795 136
Size: 1525
Fragments arranged by size:
1692 1525 1094 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 6179 6179
Size: 6184
BlpI GC'TnA_GC
Cuts at: 3601 3601
Size: 6184
Bme1580I G_kGCm'C
Cuts at: 1790 2298 3135 3411 4245 5401 1790
Size: 508 837 276 834 1156 2573
Fragments arranged by size:
2573 1156 837 834 508 276
BmgBI CAC'GTC
Cuts at: 1318 3130 3139 1318
Size: 1812 9 4363
Fragments arranged by size:
4363 1812 9
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2230 2283 3915
Size: 416 53 83 399 24 813 53 1632
Cuts at: 3915 4873 442
Size: 958 1753
Fragments arranged by size:
1753 1632 958 813 416 399 83 53
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2593 2600 3038 4844 1239
Size: 1354 7 438 1806 2579
Fragments arranged by size:
2579 1806 1354 438 7
Bpu10I CC'TnA_GC
Cuts at: 3044 3044
Size: 6184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4309 5174 5472 4309
Size: 865 298 5021
Fragments arranged by size:
5021 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4847 882
Size: 617 3348 2219
Fragments arranged by size:
3348 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2389 3975 493
Size: 711 1185 1586 2702
Fragments arranged by size:
2702 1586 1185 711
BsaBI GATnn'nnATC
Cuts at: 6178 6178
Size: 6184
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2379 2414 2451 3060
Size: 53 83 186 936 846 35 37 609
Cuts at: 3060 3366 3991 4373 5008 275
Size: 306 625 382 635 1451
Fragments arranged by size:
1451 936 846 635 625 609 382 306
186 83 53 37 35
BsaWI w'CCGG_w
Cuts at: 2018 4617 5015 5289 5436 5847 2018
Size: 2599 398 274 147 411 2355
Fragments arranged by size:
2599 2355 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2681 2711 2929 2959 1178
Size: 30 165 30 1278 30 218 30 4403
Fragments arranged by size:
4403 1278 218 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2174 2426 3035 3374 1186
Size: 988 252 609 339 3996
Fragments arranged by size:
3996 988 609 339 252
BseYI C'CCAG_C
Cuts at: 2035 5387 5883 2035
Size: 3352 496 2336
Fragments arranged by size:
3352 2336 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2144 2161 3186 1163
Size: 573 408 17 1025 4161
Fragments arranged by size:
4161 1025 573 408 17
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2797 3634 4395 5423 1540
Size: 275 186 796 837 761 1028 2301
Fragments arranged by size:
2301 1028 837 796 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2352 2688 2788 2942 3135 3231 4245 4330 5401
Size: 336 100 154 193 96 1014 85 1071
Cuts at: 5401 2352
Size: 3135
Fragments arranged by size:
3135 1071 1014 336 193 154 100 96
85
BsmI GAATG_Cn'
Cuts at: 3695 3788 3695
Size: 93 6091
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2298 2352 2688 2788 2942 3135 3180
Size: 399 508 54 336 100 154 193 45
Cuts at: 3180 3231 3411 3503 4245 4330 5401 1391
Size: 51 180 92 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 508 399 336 193 180
154 100 92 85 54 51 45
BspEI T'CCGG_A
Cuts at: 5847 5847
Size: 6184
BspHI T'CATG_A
Cuts at: 4073 5803 4073
Size: 1730 4454
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2738 2879 3219 3410 3539 844
Size: 1894 141 340 191 129 3489
Fragments arranged by size:
3489 1894 340 191 141 129
BsrBI CCG'CTC
Cuts at: 1394 1823 2101 3048 4071 1394
Size: 429 278 947 1023 3507
Fragments arranged by size:
3507 1023 947 429 278
BsrDI GCAATG_nn'
Cuts at: 65 1248 4679 4853 65
Size: 1183 3431 174 1396
Fragments arranged by size:
3431 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2082 2129 4828 5015 1700
Size: 110 272 47 2699 187 2869
Fragments arranged by size:
2869 2699 272 187 110 47
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6184
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6184
BssSI C'ACGA_G
Cuts at: 4244 5256 4244
Size: 1012 5172
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2475 794
Size: 1681 4503
BstBI TT'CG_AA
Cuts at: 2511 4988 2511
Size: 2477 3707
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6184
BstXI CCAn_nnnn'nTGG
Cuts at: 2326 2976 2326
Size: 650 5534
BstYI r'GATC_y
Cuts at: 1972 2334 3514 3881 4266 4283 5735 5821 5833
Size: 362 1180 367 385 17 1452 86 12
Cuts at: 5833 6179 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1180 385 367 362 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2370 3063 3234 513
Size: 552 939 366 693 171 3463
Fragments arranged by size:
3463 939 693 552 366 171
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5445
Fragments arranged by size:
5445 683 56
BtsI GCAGTG_nn'
Cuts at: 2476 2749 2898 2934 3198 3700 4494 4514 2476
Size: 273 149 36 264 502 794 20 4146
Fragments arranged by size:
4146 794 502 273 264 149 36 20
ClaI AT'CG_AT
Cuts at: 1554 2262 1554
Size: 708 5476
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6149
DraI TTT'AAA
Cuts at: 3488 3488
Size: 6184
DraIII CAC_nnn'GTG
Cuts at: 3139 3139
Size: 6184
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2189 3065 5191 809
Size: 507 873 876 2126 1802
Fragments arranged by size:
2126 1802 876 873 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 3109 3631
Size: 54 1425 50 275 18 183 1096 522
Cuts at: 3631 4520 6149 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1096 889 522 275 183 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3631 1537
Size: 275 1819 4090
Fragments arranged by size:
4090 1819 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2201 2684 3417 4114 1353
Size: 848 483 733 697 3423
Fragments arranged by size:
3423 848 733 697 483
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2189 3299 4771 5145 5291 387
Size: 1064 138 600 1110 1472 374 146 1280
Fragments arranged by size:
1472 1280 1110 1064 600 374 146 138
EcoICRI GAG'CTC
Cuts at: 2786 2940 2786
Size: 154 6030
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2593 2600 3038 4247 4844 5631
Size: 393 440 521 7 438 1209 597 787
Cuts at: 5631 5899 1239
Size: 268 1524
Fragments arranged by size:
1524 1209 787 597 521 440 438 393
268 7
EcoNI CCTnn'n_nnAGG
Cuts at: 3272 3272
Size: 6184
EcoO109I rG'GnC_Cy
Cuts at: 2191 3277 3319 2191
Size: 1086 42 5056
Fragments arranged by size:
5056 1086 42
EcoRI G'AATT_C
Cuts at: 3493 3871 3493
Size: 378 5806
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 6184
FspI TGC'GCA
Cuts at: 3197 4690 3197
Size: 1493 4691
HaeII r_GCGC'y
Cuts at: 1678 2069 2092 2173 3331 5331 1678
Size: 391 23 81 1158 2000 2531
Fragments arranged by size:
2531 2000 1158 391 81 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4825 4857 4899 4931 1373
Size: 32 135 32 3253 32 42 32 2626
Fragments arranged by size:
3253 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2269 2283 2455 2518 3528 3774
Size: 988 281 331 14 172 63 1010 246
Cuts at: 3774 669
Size: 3079
Fragments arranged by size:
3079 1010 988 331 281 246 172 63
14
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6184
HpaI GTT'AAC
Cuts at: 3774 3774
Size: 6184
KpnI G_GTAC'C
Cuts at: 3509 3509
Size: 6184
MfeI C'AATT_G
Cuts at: 3783 3783
Size: 6184
MluI A'CGCG_T
Cuts at: 6060 6060
Size: 6184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2649 2886 5298 5482 1305
Size: 1344 237 2412 184 2007
Fragments arranged by size:
2412 2007 1344 237 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3111 10
Size: 54 1425 343 1279 3083
Fragments arranged by size:
3083 1425 1279 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2324 3128 4142 4501 4660 518
Size: 648 1158 804 1014 359 159 2042
Fragments arranged by size:
2042 1158 1014 804 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2062 2466 3384 3600 4277 5425 5670 1606
Size: 456 404 918 216 677 1148 245 2120
Fragments arranged by size:
2120 1148 918 677 456 404 245 216
NaeI GCC'GGC
Cuts at: 2131 2131
Size: 6184
NcoI C'CATG_G
Cuts at: 513 1065 2004 3063 513
Size: 552 939 1059 3634
Fragments arranged by size:
3634 1059 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6184
NgoMIV G'CCGG_C
Cuts at: 2129 2129
Size: 6184
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6184
NotI GC'GGCC_GC
Cuts at: 3631 3631
Size: 6184
NsiI A_TGCA'T
Cuts at: 3440 3440
Size: 6184
NspI r_CATG'y
Cuts at: 1132 1229 2713 2992 3438 3542 5087 1132
Size: 97 1484 279 446 104 1545 2229
Fragments arranged by size:
2229 1545 1484 446 279 104 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6184
PciI A'CATG_T
Cuts at: 5083 5083
Size: 6184
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3118 3268 1108
Size: 2010 150 4024
Fragments arranged by size:
4024 2010 150
PfoI T'CCnGG_A
Cuts at: 3267 3267
Size: 6184
PmeI GTTT'AAAC
Cuts at: 3488 3488
Size: 6184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4253 4285 5766 5798 4253
Size: 32 1481 32 4639
Fragments arranged by size:
4639 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2191 3319 2191
Size: 1128 5056
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6184
PsiI TTA'TAA
Cuts at: 3754 3754
Size: 6184
PstI C_TGCA'G
Cuts at: 830 2151 2480 2905 3216 3327 3536 830
Size: 1321 329 425 311 111 209 3478
Fragments arranged by size:
3478 1321 425 329 311 209 111
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6184
SacI G_AGCT'C
Cuts at: 2788 2942 2788
Size: 154 6030
SalI G'TCGA_C
Cuts at: 1936 2281 3526 1936
Size: 345 1245 4594
Fragments arranged by size:
4594 1245 345
SbfI CC_TGCA'GG
Cuts at: 2151 3536 2151
Size: 1385 4799
ScaI AGT'ACT
Cuts at: 4432 6144 4432
Size: 1712 4472
SfcI C'TryA_G
Cuts at: 826 1045 2147 2476 2901 3212 3323 3532 4667
Size: 219 1102 329 425 311 111 209 1135
Cuts at: 4667 5348 5539 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1102 681 425 329 311 219
209 191 111
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6184
SmaI CCC'GGG
Cuts at: 1872 2702 3511 1872
Size: 830 809 4545
Fragments arranged by size:
4545 830 809
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4288 5189 5451 820
Size: 197 926 2345 901 262 1553
Fragments arranged by size:
2345 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2389 3975 493
Size: 1896 1586 2702
Fragments arranged by size:
2702 1896 1586
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6184
SphI G_CATG'C
Cuts at: 1132 2713 3438 3542 1132
Size: 1581 725 104 3774
Fragments arranged by size:
3774 1581 725 104
SspI AAT'ATT
Cuts at: 5 52 4108 5
Size: 47 4056 2081
Fragments arranged by size:
4056 2081 47
StuI AGG'CCT
Cuts at: 1517 2486 3458 1517
Size: 969 972 4243
Fragments arranged by size:
4243 972 969
StyI C'CwwG_G
Cuts at: 513 1065 2004 2343 3063 3480 3623 513
Size: 552 939 339 720 417 143 3074
Fragments arranged by size:
3074 939 720 552 417 339 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2550 2811 2945 4211 4228 4381 4566
Size: 1255 970 261 134 1266 17 153 185
Cuts at: 4566 5011 325
Size: 445 1498
Fragments arranged by size:
1498 1266 1255 970 445 261 185 153
134 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4430 6142 96
Size: 274 80 33 51 1443 2453 1712 138
Fragments arranged by size:
2453 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2929 2961 6144 6176 2929
Size: 32 3183 32 2937
Fragments arranged by size:
3183 2937 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2378 1316
Size: 1062 5122
XbaI T'CTAG_A
Cuts at: 3520 3520
Size: 6184
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2976 2976
Size: 6184
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6184
XmaI C'CCGG_G
Cuts at: 1870 2700 3509 1870
Size: 830 809 4545
Fragments arranged by size:
4545 830 809
XmnI GAAnn'nnTTC
Cuts at: 4313 4313
Size: 6184
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2380 2415 3367 3992
Size: 53 83 186 936 846 35 952 625
Cuts at: 3992 276
Size: 2468
Fragments arranged by size:
2468 952 936 846 625 186 83 53
35
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI
NarI NruI PacI PmlI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI