Restriction map for
pFN21AE4471
(Circular) (Six-base) MAPSORT of: pFN21AE4471.seq Check: 4118 from: 1 to: 6181
pFN21AE4471 6181 bp
With 182 enzymes: SgfI *
March 28, 2012 14:04 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3991 278
Size: 53 83 186 936 2455 2468
Fragments arranged by size:
2468 2455 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3524 1937
Size: 1587 4594
Acc65I G'GTAC_C
Cuts at: 3502 3502
Size: 6181
AclI AA'CG_TT
Cuts at: 1563 4308 4681 1563
Size: 2745 373 3063
Fragments arranged by size:
3063 2745 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2298 2645 2676 4244 5628 5896 1632
Size: 666 347 31 1568 1384 268 1917
Fragments arranged by size:
1917 1568 1384 666 347 268 31
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5984
AflIII A'CryG_T
Cuts at: 1203 5080 6057 1203
Size: 3877 977 1327
Fragments arranged by size:
3877 1327 977
AgeI A'CCGG_T
Cuts at: 5012 5012
Size: 6181
AhdI GACnn_n'nnGTC
Cuts at: 4910 4910
Size: 6181
AleI CACnn'nnGTG
Cuts at: 1166 2945 3317 1166
Size: 1779 372 4030
Fragments arranged by size:
4030 1779 372
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3140 1184
Size: 1956 4225
AlwNI CAG_nnn'CTG
Cuts at: 2276 2327 3924 5496 2276
Size: 51 1597 1572 2961
Fragments arranged by size:
2961 1597 1572 51
ApaLI G'TGCA_C
Cuts at: 4238 5394 4238
Size: 1156 5025
ApoI r'AATT_y
Cuts at: 1493 2387 2983 3068 3239 3375 3490 3716 3868
Size: 894 596 85 171 136 115 226 152
Cuts at: 3868 5977 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 894 596 226 171 152 136
115 85
AseI AT'TA_AT
Cuts at: 160 1032 2064 4735 160
Size: 872 1032 2671 1606
Fragments arranged by size:
2671 1606 1032 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6181
AvaI C'yCGr_G
Cuts at: 1870 1943 2114 3506 1870
Size: 73 171 1392 4545
Fragments arranged by size:
4545 1392 171 73
BamHI G'GATC_C
Cuts at: 3511 3878 3511
Size: 367 5814
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3124 3502 4957 618
Size: 325 217 627 1337 378 1455 1842
Fragments arranged by size:
1842 1455 1337 627 378 325 217
BanII G_rGCy'C
Cuts at: 1391 3500 1391
Size: 2109 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2216 2291 928
Size: 971 317 75 4818
Fragments arranged by size:
4818 971 317 75
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4372 4406 1903
Size: 34 2435 34 3678
Fragments arranged by size:
3678 2435 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4073 5283 4073
Size: 1210 4971
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6181
BfrBI ATG'CAT
Cuts at: 2584 2584
Size: 6181
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4792 136
Size: 107 122 71 631 605 3120 1525
Fragments arranged by size:
3120 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2439 6176 2439
Size: 3737 2444
BlpI GC'TnA_GC
Cuts at: 3598 3598
Size: 6181
Bme1580I G_kGCm'C
Cuts at: 1790 4242 5398 1790
Size: 2452 1156 2573
Fragments arranged by size:
2573 2452 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6181
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3264 3912 4870
Size: 416 53 83 399 24 1847 648 958
Cuts at: 4870 442
Size: 1753
Fragments arranged by size:
1847 1753 958 648 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3158 4841 1239
Size: 1919 1683 2579
Fragments arranged by size:
2579 1919 1683
Bpu10I CC'TnA_GC
Cuts at: 2154 2154
Size: 6181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2801 2986 4306 5171 5469 2801
Size: 185 1320 865 298 3513
Fragments arranged by size:
3513 1320 865 298 185
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3332 4844 882
Size: 617 1833 1512 2219
Fragments arranged by size:
2219 1833 1512 617
BsaAI yAC'GTr
Cuts at: 493 1204 2299 3319 3972 493
Size: 711 1095 1020 653 2702
Fragments arranged by size:
2702 1095 1020 711 653
BsaBI GATnn'nnATC
Cuts at: 6175 6175
Size: 6181
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3988 4370 5005 275
Size: 53 83 186 936 2455 382 635 1451
Fragments arranged by size:
2455 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2449 2769 4614 5012 5286 5433 5844 2449
Size: 320 1845 398 274 147 411 2786
Fragments arranged by size:
2786 1845 411 398 320 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2256 2286 2775 2805 1178
Size: 30 165 30 853 30 489 30 4554
Fragments arranged by size:
4554 853 489 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3268 1186
Size: 2082 4099
BseYI C'CCAG_C
Cuts at: 3166 5384 5880 3166
Size: 2218 496 3467
Fragments arranged by size:
3467 2218 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3042 3341 1163
Size: 573 1306 299 4003
Fragments arranged by size:
4003 1306 573 299
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3631 4392 5420 1540
Size: 275 186 1630 761 1028 2301
Fragments arranged by size:
2301 1630 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3402 4242 4327 5398 3402
Size: 840 85 1071 4185
Fragments arranged by size:
4185 1071 840 85
BsmI GAATG_Cn'
Cuts at: 3692 3785 3692
Size: 93 6088
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3402 3500 4242 4327 5398 1391
Size: 399 1612 98 742 85 1071 2174
Fragments arranged by size:
2174 1612 1071 742 399 98 85
BspEI T'CCGG_A
Cuts at: 5844 5844
Size: 6181
BspHI T'CATG_A
Cuts at: 2796 2865 4070 5800 2796
Size: 69 1205 1730 3177
Fragments arranged by size:
3177 1730 1205 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3536 844
Size: 2692 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4068 1394
Size: 429 2245 3507
Fragments arranged by size:
3507 2245 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3294 4676 4850 65
Size: 1183 2046 1382 174 1396
Fragments arranged by size:
2046 1396 1382 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2244 4825 5012 1700
Size: 110 434 2581 187 2869
Fragments arranged by size:
2869 2581 434 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6181
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6181
BssSI C'ACGA_G
Cuts at: 4241 5253 4241
Size: 1012 5169
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6181
BstBI TT'CG_AA
Cuts at: 4985 4985
Size: 6181
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6181
BstXI CCAn_nnnn'nTGG
Cuts at: 2215 2587 2753 3223 2215
Size: 372 166 470 5173
Fragments arranged by size:
5173 470 372 166
BstYI r'GATC_y
Cuts at: 1972 2406 2439 3511 3878 4263 4280 5732 5818
Size: 434 33 1072 367 385 17 1452 86
Cuts at: 5818 5830 6176 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1072 434 385 367 346 86
33 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2601 2694 3015 513
Size: 552 1536 93 321 3679
Fragments arranged by size:
3679 1536 552 321 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5442
Fragments arranged by size:
5442 683 56
BtsI GCAGTG_nn'
Cuts at: 2277 3697 4491 4511 2277
Size: 1420 794 20 3947
Fragments arranged by size:
3947 1420 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6181
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6146
DraI TTT'AAA
Cuts at: 3485 3485
Size: 6181
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5188 809
Size: 507 3872 1802
Fragments arranged by size:
3872 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3012 3225 3628
Size: 54 1425 50 275 18 1182 213 403
Cuts at: 3628 4517 6146 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1182 889 403 275 213 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3628 1537
Size: 275 1816 4090
Fragments arranged by size:
4090 1816 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4111 1353
Size: 2758 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4768 5142 5288 387
Size: 1064 138 3179 374 146 1280
Fragments arranged by size:
3179 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2298 2645 2676 3158 4244 4841 5628
Size: 393 666 347 31 482 1086 597 787
Cuts at: 5628 5896 1239
Size: 268 1524
Fragments arranged by size:
1524 1086 787 666 597 482 393 347
268 31
EcoNI CCTnn'n_nnAGG
Cuts at: 3227 3227
Size: 6181
EcoO109I rG'GnC_Cy
Cuts at: 3133 3133
Size: 6181
EcoRI G'AATT_C
Cuts at: 3490 3868 3490
Size: 378 5803
FspI TGC'GCA
Cuts at: 4687 4687
Size: 6181
HaeII r_GCGC'y
Cuts at: 1678 3264 5328 1678
Size: 1586 2064 2531
Fragments arranged by size:
2531 2064 1586
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2775 2807 3196 3228 4822
Size: 32 135 32 1203 32 389 32 1594
Cuts at: 4822 4854 4896 4928 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1594 1203 389 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2559 3082 3370 3442 3525 3771
Size: 988 281 621 523 288 72 83 246
Cuts at: 3771 669
Size: 3079
Fragments arranged by size:
3079 988 621 523 288 281 246 83
72
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6181
HpaI GTT'AAC
Cuts at: 2559 3771 2559
Size: 1212 4969
KpnI G_GTAC'C
Cuts at: 3506 3506
Size: 6181
MfeI C'AATT_G
Cuts at: 3780 3780
Size: 6181
MluI A'CGCG_T
Cuts at: 6057 6057
Size: 6181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5295 5479 1305
Size: 3990 184 2007
Fragments arranged by size:
3990 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3227 10
Size: 54 1425 343 1395 2964
Fragments arranged by size:
2964 1425 1395 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2297 2330 2606 2945 3317 4139 4498
Size: 648 1131 33 276 339 372 822 359
Cuts at: 4498 4657 518
Size: 159 2042
Fragments arranged by size:
2042 1131 822 648 372 359 339 276
159 33
MspA1I CmG'CkG
Cuts at: 1606 2654 3597 4274 5422 5667 1606
Size: 1048 943 677 1148 245 2120
Fragments arranged by size:
2120 1148 1048 943 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2601 3015 513
Size: 552 1536 414 3679
Fragments arranged by size:
3679 1536 552 414
NdeI CA'TA_TG
Cuts at: 387 3004 3436 387
Size: 2617 432 3132
Fragments arranged by size:
3132 2617 432
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6181
NotI GC'GGCC_GC
Cuts at: 3628 3628
Size: 6181
NsiI A_TGCA'T
Cuts at: 2586 2586
Size: 6181
NspI r_CATG'y
Cuts at: 1132 1229 2162 2615 3539 5084 1132
Size: 97 933 453 924 1545 2229
Fragments arranged by size:
2229 1545 933 924 453 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6181
PciI A'CATG_T
Cuts at: 5080 5080
Size: 6181
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3021 1108
Size: 1913 4268
PmeI GTTT'AAAC
Cuts at: 3485 3485
Size: 6181
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2898 2930 4250 4282 5763 5795 2898
Size: 32 1320 32 1481 32 3284
Fragments arranged by size:
3284 1481 1320 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6181
PsiI TTA'TAA
Cuts at: 3751 3751
Size: 6181
PstI C_TGCA'G
Cuts at: 830 3533 830
Size: 2703 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6181
PvuII CAG'CTG
Cuts at: 2654 2654
Size: 6181
SalI G'TCGA_C
Cuts at: 1936 3523 1936
Size: 1587 4594
SbfI CC_TGCA'GG
Cuts at: 3533 3533
Size: 6181
ScaI AGT'ACT
Cuts at: 4429 6141 4429
Size: 1712 4469
SfcI C'TryA_G
Cuts at: 826 1045 3529 4664 5345 5536 826
Size: 219 2484 1135 681 191 1471
Fragments arranged by size:
2484 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6181
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6181
SmaI CCC'GGG
Cuts at: 1872 2116 3508 1872
Size: 244 1392 4545
Fragments arranged by size:
4545 1392 244
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2816 2965 4285 5186 5448 820
Size: 197 926 873 149 1320 901 262 1553
Fragments arranged by size:
1553 1320 926 901 873 262 197 149
SnaBI TAC'GTA
Cuts at: 493 3972 493
Size: 3479 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6181
SphI G_CATG'C
Cuts at: 1132 2162 2615 3539 1132
Size: 1030 453 924 3774
Fragments arranged by size:
3774 1030 924 453
SspI AAT'ATT
Cuts at: 5 52 2068 4105 5
Size: 47 2016 2037 2081
Fragments arranged by size:
2081 2037 2016 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6181
StyI C'CwwG_G
Cuts at: 513 1065 2044 2601 3015 3228 3255 3306 3620
Size: 552 979 557 414 213 27 51 314
Cuts at: 3620 513
Size: 3074
Fragments arranged by size:
3074 979 557 552 414 314 213 51
27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3319 4208 4225 4378 4563 5008 325
Size: 1255 1739 889 17 153 185 445 1498
Fragments arranged by size:
1739 1498 1255 889 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2337 2777 4427
Size: 274 80 33 51 1443 360 440 1650
Cuts at: 4427 6139 96
Size: 1712 138
Fragments arranged by size:
1712 1650 1443 440 360 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2256 2288 6141 6173 2256
Size: 32 3853 32 2264
Fragments arranged by size:
3853 2264 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6181
XbaI T'CTAG_A
Cuts at: 3517 3517
Size: 6181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3415 3415
Size: 6181
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6181
XmaI C'CCGG_G
Cuts at: 1870 2114 3506 1870
Size: 244 1392 4545
Fragments arranged by size:
4545 1392 244
XmnI GAAnn'nnTTC
Cuts at: 2595 4310 2595
Size: 1715 4466
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3989 276
Size: 53 83 186 936 2455 2468
Fragments arranged by size:
2468 2455 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI