Restriction map for
pFN21AE4469
(Circular) (Six-base) MAPSORT of: pFN21AE4469.seq Check: 6094 from: 1 to: 6175
pFN21AE4469 6175 bp
With 182 enzymes: SgfI *
March 28, 2012 14:03 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3985 278
Size: 53 83 186 936 2449 2468
Fragments arranged by size:
2468 2449 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3518 1937
Size: 1581 4594
Acc65I G'GTAC_C
Cuts at: 3496 3496
Size: 6175
AclI AA'CG_TT
Cuts at: 1563 4302 4675 1563
Size: 2739 373 3063
Fragments arranged by size:
3063 2739 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2602 2966 4238 5622 5890 1632
Size: 970 364 1272 1384 268 1917
Fragments arranged by size:
1917 1384 1272 970 364 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5978
AflIII A'CryG_T
Cuts at: 1203 5074 6051 1203
Size: 3871 977 1327
Fragments arranged by size:
3871 1327 977
AgeI A'CCGG_T
Cuts at: 3095 5006 3095
Size: 1911 4264
AhdI GACnn_n'nnGTC
Cuts at: 4904 4904
Size: 6175
AleI CACnn'nnGTG
Cuts at: 1166 3098 1166
Size: 1932 4243
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6175
AlwNI CAG_nnn'CTG
Cuts at: 3918 5490 3918
Size: 1572 4603
ApaLI G'TGCA_C
Cuts at: 3059 4232 5388 3059
Size: 1173 1156 3846
Fragments arranged by size:
3846 1173 1156
ApoI r'AATT_y
Cuts at: 1493 2401 2581 2846 3203 3303 3484 3710 3862
Size: 908 180 265 357 100 181 226 152
Cuts at: 3862 5971 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 908 357 265 226 181 180
152 100
AseI AT'TA_AT
Cuts at: 160 1032 4729 160
Size: 872 3697 1606
Fragments arranged by size:
3697 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6175
AvaI C'yCGr_G
Cuts at: 1870 1943 2784 3500 1870
Size: 73 841 716 4545
Fragments arranged by size:
4545 841 716 73
BamHI G'GATC_C
Cuts at: 2640 3505 3872 2640
Size: 865 367 4943
Fragments arranged by size:
4943 865 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3496 4951 618
Size: 325 217 627 1709 1455 1842
Fragments arranged by size:
1842 1709 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3014 3494 1391
Size: 1623 480 4072
Fragments arranged by size:
4072 1623 480
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5204
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4366 4400 1903
Size: 34 2429 34 3678
Fragments arranged by size:
3678 2429 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4067 5277 4067
Size: 1210 4965
BclI T'GATC_A
Cuts at: 1548 2808 2986 1548
Size: 1260 178 4737
Fragments arranged by size:
4737 1260 178
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4786 136
Size: 107 122 71 631 605 3114 1525
Fragments arranged by size:
3114 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3456 6170 3456
Size: 2714 3461
BlpI GC'TnA_GC
Cuts at: 3592 3592
Size: 6175
Bme1580I G_kGCm'C
Cuts at: 1790 3063 3105 3158 4236 5392 1790
Size: 1273 42 53 1078 1156 2573
Fragments arranged by size:
2573 1273 1156 1078 53 42
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6175
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3906 4864 442
Size: 416 53 83 399 24 2489 958 1753
Fragments arranged by size:
2489 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2083 4835 1239
Size: 844 2752 2579
Fragments arranged by size:
2752 2579 844
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4300 5165 5463 4300
Size: 865 298 5012
Fragments arranged by size:
5012 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4838 882
Size: 617 3339 2219
Fragments arranged by size:
3339 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3966 493
Size: 711 2762 2702
Fragments arranged by size:
2762 2702 711
BsaBI GATnn'nnATC
Cuts at: 6169 6169
Size: 6175
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3982 4364 4999 275
Size: 53 83 186 936 2449 382 635 1451
Fragments arranged by size:
2449 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3095 4608 5006 5280 5427 5838 3095
Size: 1513 398 274 147 411 3432
Fragments arranged by size:
3432 1513 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2922 2952 1178
Size: 30 165 30 1519 30 4401
Fragments arranged by size:
4401 1519 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2097 2294 2434 2480 1186
Size: 911 197 140 46 4881
Fragments arranged by size:
4881 911 197 140 46
BseYI C'CCAG_C
Cuts at: 5378 5874 5378
Size: 496 5679
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3330 1163
Size: 573 1594 4008
Fragments arranged by size:
4008 1594 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3625 4386 5414 1540
Size: 275 186 1624 761 1028 2301
Fragments arranged by size:
2301 1624 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2524 3063 4236 4321 5392 2524
Size: 539 1173 85 1071 3307
Fragments arranged by size:
3307 1173 1071 539 85
BsmI GAATG_Cn'
Cuts at: 3686 3779 3686
Size: 93 6082
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2524 3014 3063 3105 3158 3494 4236
Size: 399 734 490 49 42 53 336 742
Cuts at: 4236 4321 5392 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 734 490 399 336 85
53 49 42
BspEI T'CCGG_A
Cuts at: 5838 5838
Size: 6175
BspHI T'CATG_A
Cuts at: 4064 5794 4064
Size: 1730 4445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3530 844
Size: 2686 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4062 1394
Size: 429 2239 3507
Fragments arranged by size:
3507 2239 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2356 2536 4670 4844 65
Size: 1183 1108 180 2134 174 1396
Fragments arranged by size:
2134 1396 1183 1108 180 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3095 4819 5006 1700
Size: 110 1285 1724 187 2869
Fragments arranged by size:
2869 1724 1285 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6175
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6175
BssSI C'ACGA_G
Cuts at: 3062 4235 5247 3062
Size: 1173 1012 3990
Fragments arranged by size:
3990 1173 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6175
BstBI TT'CG_AA
Cuts at: 4979 4979
Size: 6175
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6175
BstYI r'GATC_y
Cuts at: 1972 2370 2640 3066 3456 3505 3872 4257 4274
Size: 398 270 426 390 49 367 385 17
Cuts at: 4274 5726 5812 5824 6170 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 426 398 390 385 367 346
270 86 49 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2636 513
Size: 552 939 632 4052
Fragments arranged by size:
4052 939 632 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5436
Fragments arranged by size:
5436 683 56
BtsI GCAGTG_nn'
Cuts at: 2452 3117 3691 4485 4505 2452
Size: 665 574 794 20 4122
Fragments arranged by size:
4122 794 665 574 20
ClaI AT'CG_AT
Cuts at: 1554 2796 1554
Size: 1242 4933
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6140
DraI TTT'AAA
Cuts at: 3396 3479 3396
Size: 83 6092
DraIII CAC_nnn'GTG
Cuts at: 2495 2495
Size: 6175
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5182 809
Size: 507 3866 1802
Fragments arranged by size:
3866 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3232 3622 4511
Size: 54 1425 50 275 18 1402 390 889
Cuts at: 4511 6140 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1402 889 390 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3622 1537
Size: 275 1810 4090
Fragments arranged by size:
4090 1810 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2115 2287 2411 2973 4105 1353
Size: 762 172 124 562 1132 3423
Fragments arranged by size:
3423 1132 762 562 172 124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4762 5136 5282 387
Size: 1064 138 3173 374 146 1280
Fragments arranged by size:
3173 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2083 2602 2966 4238 4835 5622 5890
Size: 393 451 519 364 1272 597 787 268
Cuts at: 5890 1239
Size: 1524
Fragments arranged by size:
1524 1272 787 597 519 451 393 364
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2462 2462
Size: 6175
EcoRI G'AATT_C
Cuts at: 2846 3484 3862 2846
Size: 638 378 5159
Fragments arranged by size:
5159 638 378
FspI TGC'GCA
Cuts at: 4681 4681
Size: 6175
HaeII r_GCGC'y
Cuts at: 1678 5322 1678
Size: 3644 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2322 2354 2916 2922 2948
Size: 32 135 32 750 32 562 6 26
Cuts at: 2948 2954 3421 3453 4816 4848 4890 4922 1373
Size: 6 467 32 1363 32 42 32 2626
Fragments arranged by size:
2626 1363 750 562 467 135 42 32
32 32 32 32 32 26 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2776 3519 3765 669
Size: 988 281 838 743 246 3079
Fragments arranged by size:
3079 988 838 743 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6175
HpaI GTT'AAC
Cuts at: 2776 3765 2776
Size: 989 5186
KpnI G_GTAC'C
Cuts at: 3500 3500
Size: 6175
MfeI C'AATT_G
Cuts at: 3774 3774
Size: 6175
MluI A'CGCG_T
Cuts at: 6051 6051
Size: 6175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5289 5473 1305
Size: 3984 184 2007
Fragments arranged by size:
3984 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3234 10
Size: 54 1425 343 1402 2951
Fragments arranged by size:
2951 1425 1402 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2228 2241 2594 3098 4133 4492 4651
Size: 648 1062 13 353 504 1035 359 159
Cuts at: 4651 518
Size: 2042
Fragments arranged by size:
2042 1062 1035 648 504 359 353 159
13
MspA1I CmG'CkG
Cuts at: 1606 3591 4268 5416 5661 1606
Size: 1985 677 1148 245 2120
Fragments arranged by size:
2120 1985 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2636 513
Size: 552 939 632 4052
Fragments arranged by size:
4052 939 632 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6175
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6175
NotI GC'GGCC_GC
Cuts at: 3622 3622
Size: 6175
NspI r_CATG'y
Cuts at: 1132 1229 3022 3040 3533 5078 1132
Size: 97 1793 18 493 1545 2229
Fragments arranged by size:
2229 1793 1545 493 97 18
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6175
PciI A'CATG_T
Cuts at: 5074 5074
Size: 6175
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6175
PfoI T'CCnGG_A
Cuts at: 2973 2973
Size: 6175
PmeI GTTT'AAAC
Cuts at: 3479 3479
Size: 6175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4244 4276 5757 5789 4244
Size: 32 1481 32 4630
Fragments arranged by size:
4630 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6175
PsiI TTA'TAA
Cuts at: 2571 3745 2571
Size: 1174 5001
PstI C_TGCA'G
Cuts at: 830 3418 3527 830
Size: 2588 109 3478
Fragments arranged by size:
3478 2588 109
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6175
SalI G'TCGA_C
Cuts at: 1936 3517 1936
Size: 1581 4594
SbfI CC_TGCA'GG
Cuts at: 3527 3527
Size: 6175
ScaI AGT'ACT
Cuts at: 4423 6135 4423
Size: 1712 4463
SfcI C'TryA_G
Cuts at: 826 1045 2074 2132 2874 3054 3414 3523 4658
Size: 219 1029 58 742 180 360 109 1135
Cuts at: 4658 5339 5530 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1029 742 681 360 219 191
180 109 58
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6175
SgrAI Cr'CCGG_yG
Cuts at: 1700 3095 1700
Size: 1395 4780
SmaI CCC'GGG
Cuts at: 1872 3502 1872
Size: 1630 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4279 5180 5442 820
Size: 197 926 2336 901 262 1553
Fragments arranged by size:
2336 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3966 493
Size: 3473 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6175
SphI G_CATG'C
Cuts at: 1132 3022 3533 1132
Size: 1890 511 3774
Fragments arranged by size:
3774 1890 511
SspI AAT'ATT
Cuts at: 5 52 2854 4099 5
Size: 47 2802 1245 2081
Fragments arranged by size:
2802 2081 1245 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6175
StyI C'CwwG_G
Cuts at: 513 1065 2004 2481 2636 3150 3614 513
Size: 552 939 477 155 514 464 3074
Fragments arranged by size:
3074 939 552 514 477 464 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2967 4202 4219 4372 4557 5002 325
Size: 1255 1387 1235 17 153 185 445 1498
Fragments arranged by size:
1498 1387 1255 1235 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4421 6133 96
Size: 274 80 33 51 1443 2444 1712 138
Fragments arranged by size:
2444 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2095 2127 6135 6167 2095
Size: 32 4008 32 2103
Fragments arranged by size:
4008 2103 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3086 1316
Size: 1770 4405
XbaI T'CTAG_A
Cuts at: 3241 3511 3241
Size: 270 5905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2509 2509
Size: 6175
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6175
XmaI C'CCGG_G
Cuts at: 1870 3500 1870
Size: 1630 4545
XmnI GAAnn'nnTTC
Cuts at: 2341 2603 4304 2341
Size: 262 1701 4212
Fragments arranged by size:
4212 1701 262
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3983 276
Size: 53 83 186 936 2449 2468
Fragments arranged by size:
2468 2449 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI