Restriction map for
pFN21AE4467
(Circular) (Six-base) MAPSORT of: pFN21AE4467.seq Check: 495 from: 1 to: 6172
pFN21AE4467 6172 bp
With 182 enzymes: SgfI *
March 28, 2012 14:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2026 2026
Size: 6172
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3982 278
Size: 53 83 186 936 2446 2468
Fragments arranged by size:
2468 2446 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2859 2916 3515 1937
Size: 922 57 599 4594
Fragments arranged by size:
4594 922 599 57
Acc65I G'GTAC_C
Cuts at: 3493 3493
Size: 6172
AclI AA'CG_TT
Cuts at: 1563 4299 4672 1563
Size: 2736 373 3063
Fragments arranged by size:
3063 2736 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2572 2633 3477 4235 5619 5887 1632
Size: 940 61 844 758 1384 268 1917
Fragments arranged by size:
1917 1384 940 844 758 268 61
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5975
AflIII A'CryG_T
Cuts at: 1203 3213 5071 6048 1203
Size: 2010 1858 977 1327
Fragments arranged by size:
2010 1858 1327 977
AgeI A'CCGG_T
Cuts at: 5003 5003
Size: 6172
AhdI GACnn_n'nnGTC
Cuts at: 3364 4901 3364
Size: 1537 4635
AleI CACnn'nnGTG
Cuts at: 1166 2213 2285 1166
Size: 1047 72 5053
Fragments arranged by size:
5053 1047 72
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6172
AlwNI CAG_nnn'CTG
Cuts at: 2524 2936 3090 3915 5487 2524
Size: 412 154 825 1572 3209
Fragments arranged by size:
3209 1572 825 412 154
ApaI G_GGCC'C
Cuts at: 2169 2169
Size: 6172
ApaLI G'TGCA_C
Cuts at: 2867 4229 5385 2867
Size: 1362 1156 3654
Fragments arranged by size:
3654 1362 1156
ApoI r'AATT_y
Cuts at: 1493 3481 3707 3859 5968 1493
Size: 1988 226 152 2109 1697
Fragments arranged by size:
2109 1988 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4726 160
Size: 872 3694 1606
Fragments arranged by size:
3694 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6172
AvaI C'yCGr_G
Cuts at: 1870 1943 2063 3079 3497 1870
Size: 73 120 1016 418 4545
Fragments arranged by size:
4545 1016 418 120 73
BamHI G'GATC_C
Cuts at: 3502 3869 3502
Size: 367 5805
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2038 2082 2245 2954
Size: 325 217 627 221 30 44 163 709
Cuts at: 2954 3493 4948 618
Size: 539 1455 1842
Fragments arranged by size:
1842 1455 709 627 539 325 221 217
163 44 30
BanII G_rGCy'C
Cuts at: 1391 2070 2169 2307 2376 3189 3491 1391
Size: 679 99 138 69 813 302 4072
Fragments arranged by size:
4072 813 679 302 138 99 69
BbeI G_GCGC'C
Cuts at: 2086 2958 2086
Size: 872 5300
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2821 3046 3193 928
Size: 971 922 225 147 3907
Fragments arranged by size:
3907 971 922 225 147
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4363 4397 1903
Size: 34 2426 34 3678
Fragments arranged by size:
3678 2426 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4064 5274 4064
Size: 1210 4962
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6172
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3041 4783 136
Size: 107 122 71 631 605 1369 1742 1525
Fragments arranged by size:
1742 1525 1369 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6167 6167
Size: 6172
BlpI GC'TnA_GC
Cuts at: 2306 3589 2306
Size: 1283 4889
Bme1580I G_kGCm'C
Cuts at: 1790 2013 2043 2169 2250 2871 3147 3196 4233
Size: 223 30 126 81 621 276 49 1037
Cuts at: 4233 5389 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1037 621 276 223 126 81
49 30
BmgBI CAC'GTC
Cuts at: 1318 2964 3265 1318
Size: 1646 301 4225
Fragments arranged by size:
4225 1646 301
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3903 4861 442
Size: 416 53 83 399 24 2486 958 1753
Fragments arranged by size:
2486 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1999 2705 4832 1239
Size: 760 706 2127 2579
Fragments arranged by size:
2579 2127 760 706
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4297 5162 5460 4297
Size: 865 298 5009
Fragments arranged by size:
5009 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4835 882
Size: 617 3336 2219
Fragments arranged by size:
3336 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2866 3963 493
Size: 711 1662 1097 2702
Fragments arranged by size:
2702 1662 1097 711
BsaBI GATnn'nnATC
Cuts at: 6166 6166
Size: 6172
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2083 2955 3027 3979
Size: 53 83 186 936 550 872 72 952
Cuts at: 3979 4361 4996 275
Size: 382 635 1451
Fragments arranged by size:
1451 952 936 872 635 550 382 186
83 72 53
BsaWI w'CCGG_w
Cuts at: 2070 4605 5003 5277 5424 5835 2070
Size: 2535 398 274 147 411 2407
Fragments arranged by size:
2535 2407 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5947
Fragments arranged by size:
5947 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6172
BseYI C'CCAG_C
Cuts at: 2020 2338 2488 2512 2716 2776 2950 2994 5375
Size: 318 150 24 204 60 174 44 2381
Cuts at: 5375 5871 2020
Size: 496 2321
Fragments arranged by size:
2381 2321 496 318 204 174 150 60
44 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5599
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2653 2798 2841 3622 4383 5411
Size: 275 186 652 145 43 781 761 1028
Cuts at: 5411 1540
Size: 2301
Fragments arranged by size:
2301 1028 781 761 652 275 186 145
43
BsiHKAI G_wGCw'C
Cuts at: 2070 2376 2871 3189 4233 4318 5389 2070
Size: 306 495 318 1044 85 1071 2853
Fragments arranged by size:
2853 1071 1044 495 318 306 85
BsmI GAATG_Cn'
Cuts at: 3683 3776 3683
Size: 93 6079
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2043 2070 2169 2250 2307 2376
Size: 399 223 30 27 99 81 57 69
Cuts at: 2376 2871 3147 3189 3196 3491 4233 4318 5389
Size: 495 276 42 7 295 742 85 1071
Cuts at: 5389 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 495 399 295 276 223
99 85 81 69 57 42 30 27
7
BspEI T'CCGG_A
Cuts at: 5835 5835
Size: 6172
BspHI T'CATG_A
Cuts at: 4061 5791 4061
Size: 1730 4442
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2026 2182 2280 3527 844
Size: 1182 156 98 1247 3489
Fragments arranged by size:
3489 1247 1182 156 98
BsrBI CCG'CTC
Cuts at: 1394 1823 4059 1394
Size: 429 2236 3507
Fragments arranged by size:
3507 2236 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4667 4841 65
Size: 1183 3419 174 1396
Fragments arranged by size:
3419 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2212 2284 2462 2840 4816 5003 1700
Size: 110 402 72 178 378 1976 187 2869
Fragments arranged by size:
2869 1976 402 378 187 178 110 72
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6172
BssHII G'CGCG_C
Cuts at: 1925 2399 2444 2473 2999 1925
Size: 474 45 29 526 5098
Fragments arranged by size:
5098 526 474 45 29
BssSI C'ACGA_G
Cuts at: 2087 2432 3140 4232 5244 2087
Size: 345 708 1092 1012 3015
Fragments arranged by size:
3015 1092 1012 708 345
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2936 794
Size: 2142 4030
BstBI TT'CG_AA
Cuts at: 4976 4976
Size: 6172
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6172
BstXI CCAn_nnnn'nTGG
Cuts at: 2950 2950
Size: 6172
BstYI r'GATC_y
Cuts at: 1972 2988 3502 3869 4254 4271 5723 5809 5821
Size: 1016 514 367 385 17 1452 86 12
Cuts at: 5821 6167 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1016 514 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3422 3422
Size: 6172
BtgI C'CryG_G
Cuts at: 513 1065 2004 2043 2391 2973 3121 3436 513
Size: 552 939 39 348 582 148 315 3249
Fragments arranged by size:
3249 939 582 552 348 315 148 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2389 3004 3133 487
Size: 683 56 1163 615 129 3526
Fragments arranged by size:
3526 1163 683 615 129 56
BtsI GCAGTG_nn'
Cuts at: 3688 4482 4502 3688
Size: 794 20 5358
Fragments arranged by size:
5358 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6172
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6137
DraI TTT'AAA
Cuts at: 3476 3476
Size: 6172
DraIII CAC_nnn'GTG
Cuts at: 2350 2350
Size: 6172
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5179 809
Size: 507 3863 1802
Fragments arranged by size:
3863 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2129 2235 2394
Size: 54 1425 50 275 18 299 106 159
Cuts at: 2394 2460 2795 2838 3033 3619 4508 6137 8
Size: 66 335 43 195 586 889 1629 43
Fragments arranged by size:
1629 1425 889 586 335 299 275 195
159 106 66 54 50 43 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2795 2838 3619 1537
Size: 275 983 43 781 4090
Fragments arranged by size:
4090 983 781 275 43
EarI CTCTTCn'nnn_
Cuts at: 1353 2381 2612 2639 4102 1353
Size: 1028 231 27 1463 3423
Fragments arranged by size:
3423 1463 1028 231 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4759 5133 5279 387
Size: 1064 138 3170 374 146 1280
Fragments arranged by size:
3170 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2068 2374 3187 2068
Size: 306 813 5053
Fragments arranged by size:
5053 813 306
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1999 2572 2633 2705 3477 4235 4832
Size: 393 367 573 61 72 772 758 597
Cuts at: 4832 5619 5887 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 772 758 597 573 393 367
268 72 61
EcoO109I rG'GnC_Cy
Cuts at: 2165 3271 2165
Size: 1106 5066
EcoRI G'AATT_C
Cuts at: 3481 3859 3481
Size: 378 5794
FspI TGC'GCA
Cuts at: 4678 4678
Size: 6172
HaeII r_GCGC'y
Cuts at: 1678 2086 2620 2958 5319 1678
Size: 408 534 338 2361 2531
Fragments arranged by size:
2531 2361 534 408 338
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2057 2089 2924 2956 3350
Size: 32 135 32 485 32 835 32 394
Cuts at: 3350 3382 4813 4845 4887 4919 1373
Size: 32 1431 32 42 32 2626
Fragments arranged by size:
2626 1431 835 485 394 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3211 3516 3762 669
Size: 988 281 1273 305 246 3079
Fragments arranged by size:
3079 1273 988 305 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6172
HpaI GTT'AAC
Cuts at: 3762 3762
Size: 6172
KasI G'GCGC_C
Cuts at: 2082 2954 2082
Size: 872 5300
KpnI G_GTAC'C
Cuts at: 3497 3497
Size: 6172
MfeI C'AATT_G
Cuts at: 3771 3771
Size: 6172
MluI A'CGCG_T
Cuts at: 6048 6048
Size: 6172
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5286 5470 1305
Size: 3981 184 2007
Fragments arranged by size:
3981 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4350
Fragments arranged by size:
4350 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2213 2285 4130 4489 4648 518
Size: 648 1047 72 1845 359 159 2042
Fragments arranged by size:
2042 1845 1047 648 359 159 72
MspA1I CmG'CkG
Cuts at: 1606 2024 2521 2803 2975 2994 3123 3384 3588
Size: 418 497 282 172 19 129 261 204
Cuts at: 3588 4265 5413 5658 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 497 418 282 261 245
204 172 129 19
NaeI GCC'GGC
Cuts at: 2464 2842 2464
Size: 378 5794
NarI GG'CG_CC
Cuts at: 2083 2955 2083
Size: 872 5300
NcoI C'CATG_G
Cuts at: 513 1065 2004 2391 3436 513
Size: 552 939 387 1045 3249
Fragments arranged by size:
3249 1045 939 552 387
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6172
NgoMIV G'CCGG_C
Cuts at: 2462 2840 2462
Size: 378 5794
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6172
NotI GC'GGCC_GC
Cuts at: 2795 3619 2795
Size: 824 5348
NspI r_CATG'y
Cuts at: 1132 1229 2362 3314 3530 5075 1132
Size: 97 1133 952 216 1545 2229
Fragments arranged by size:
2229 1545 1133 952 216 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6172
PciI A'CATG_T
Cuts at: 5071 5071
Size: 6172
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2350 2458 2524 1108
Size: 1242 108 66 4756
Fragments arranged by size:
4756 1242 108 66
PmeI GTTT'AAAC
Cuts at: 3476 3476
Size: 6172
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4241 4273 5754 5786 4241
Size: 32 1481 32 4627
Fragments arranged by size:
4627 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6172
PsiI TTA'TAA
Cuts at: 3742 3742
Size: 6172
PspOMI G'GGCC_C
Cuts at: 2165 2165
Size: 6172
PstI C_TGCA'G
Cuts at: 830 2301 3524 830
Size: 1471 1223 3478
Fragments arranged by size:
3478 1471 1223
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6172
PvuII CAG'CTG
Cuts at: 2521 2803 2521
Size: 282 5890
RsrII CG'GwC_CG
Cuts at: 3286 3286
Size: 6172
SacI G_AGCT'C
Cuts at: 2070 2376 3189 2070
Size: 306 813 5053
Fragments arranged by size:
5053 813 306
SacII CC_GC'GG
Cuts at: 2976 3124 2976
Size: 148 6024
SalI G'TCGA_C
Cuts at: 1936 3514 1936
Size: 1578 4594
SapI GCTCTTCn'nnn_
Cuts at: 2381 2381
Size: 6172
SbfI CC_TGCA'GG
Cuts at: 3524 3524
Size: 6172
ScaI AGT'ACT
Cuts at: 4420 6132 4420
Size: 1712 4460
SexAI A'CCwGG_T
Cuts at: 2850 2850
Size: 6172
SfcI C'TryA_G
Cuts at: 826 1045 2297 2380 3520 4655 5336 5527 826
Size: 219 1252 83 1140 1135 681 191 1471
Fragments arranged by size:
1471 1252 1140 1135 681 219 191 83
SfoI GGC'GCC
Cuts at: 2084 2956 2084
Size: 872 5300
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6172
SgrAI Cr'CCGG_yG
Cuts at: 1700 2212 2284 1700
Size: 512 72 5588
Fragments arranged by size:
5588 512 72
SmaI CCC'GGG
Cuts at: 1872 3499 1872
Size: 1627 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4276 5177 5439 820
Size: 197 926 2333 901 262 1553
Fragments arranged by size:
2333 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3963 493
Size: 3470 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6172
SphI G_CATG'C
Cuts at: 1132 3530 1132
Size: 2398 3774
SspI AAT'ATT
Cuts at: 5 52 4096 5
Size: 47 4044 2081
Fragments arranged by size:
4044 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6172
StyI C'CwwG_G
Cuts at: 513 1065 2004 2391 3436 3611 513
Size: 552 939 387 1045 175 3074
Fragments arranged by size:
3074 1045 939 552 387 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4199 4216 4369 4554 4999 325
Size: 1255 2619 17 153 185 445 1498
Fragments arranged by size:
2619 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4418 6130 96
Size: 274 80 33 51 1443 2441 1712 138
Fragments arranged by size:
2441 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6132 6164 6132
Size: 32 6140
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6172
XbaI T'CTAG_A
Cuts at: 3508 3508
Size: 6172
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2023 2509 2023
Size: 486 5686
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6172
XmaI C'CCGG_G
Cuts at: 1870 3497 1870
Size: 1627 4545
XmnI GAAnn'nnTTC
Cuts at: 4301 4301
Size: 6172
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3980 276
Size: 53 83 186 936 2446 2468
Fragments arranged by size:
2468 2446 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI SanDI SfiI SrfI SwaI