Restriction map for
pFN21AE4466
(Circular) (Six-base) MAPSORT of: pFN21AE4466.seq Check: 6664 from: 1 to: 6169
pFN21AE4466 6169 bp
With 182 enzymes: SgfI *
March 28, 2012 14:03 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2646 3979 278
Size: 53 83 186 936 1110 1333 2468
Fragments arranged by size:
2468 1333 1110 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3512 1937
Size: 1575 4594
Acc65I G'GTAC_C
Cuts at: 3490 3490
Size: 6169
AclI AA'CG_TT
Cuts at: 1563 4296 4669 1563
Size: 2733 373 3063
Fragments arranged by size:
3063 2733 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2233 2399 2996 4232 5616 5884 1632
Size: 601 166 597 1236 1384 268 1917
Fragments arranged by size:
1917 1384 1236 601 597 268 166
AfeI AGC'GCT
Cuts at: 2831 2831
Size: 6169
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5972
AflIII A'CryG_T
Cuts at: 1203 5068 6045 1203
Size: 3865 977 1327
Fragments arranged by size:
3865 1327 977
AgeI A'CCGG_T
Cuts at: 5000 5000
Size: 6169
AhdI GACnn_n'nnGTC
Cuts at: 4898 4898
Size: 6169
AleI CACnn'nnGTG
Cuts at: 1166 2576 1166
Size: 1410 4759
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6169
AlwNI CAG_nnn'CTG
Cuts at: 2546 3912 5484 2546
Size: 1366 1572 3231
Fragments arranged by size:
3231 1572 1366
ApaI G_GGCC'C
Cuts at: 2117 2117
Size: 6169
ApaLI G'TGCA_C
Cuts at: 3146 4226 5382 3146
Size: 1080 1156 3933
Fragments arranged by size:
3933 1156 1080
ApoI r'AATT_y
Cuts at: 1493 3478 3704 3856 5965 1493
Size: 1985 226 152 2109 1697
Fragments arranged by size:
2109 1985 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4723 160
Size: 872 3691 1606
Fragments arranged by size:
3691 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6169
AvaI C'yCGr_G
Cuts at: 1870 1943 2053 3494 1870
Size: 73 110 1441 4545
Fragments arranged by size:
4545 1441 110 73
BamHI G'GATC_C
Cuts at: 2383 2847 3499 3866 2383
Size: 464 652 367 4686
Fragments arranged by size:
4686 652 464 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2028 2205 2474 2655 3490
Size: 325 217 627 241 177 269 181 835
Cuts at: 3490 4945 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 835 627 325 269 241 217
181 177
BanII G_rGCy'C
Cuts at: 1391 2117 2585 2799 3060 3488 1391
Size: 726 468 214 261 428 4072
Fragments arranged by size:
4072 726 468 428 261 214
BbeI G_GCGC'C
Cuts at: 2032 2032
Size: 6169
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5198
BbvCI CC'TCA_GC
Cuts at: 2201 2201
Size: 6169
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4360 4394 1903
Size: 34 2423 34 3678
Fragments arranged by size:
3678 2423 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4061 5271 4061
Size: 1210 4959
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6169
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2064 2519 3458
Size: 107 122 71 631 605 392 455 939
Cuts at: 3458 4780 136
Size: 1322 1525
Fragments arranged by size:
1525 1322 939 631 605 455 392 122
107 71
BglII A'GATC_T
Cuts at: 6164 6164
Size: 6169
BlpI GC'TnA_GC
Cuts at: 3054 3586 3054
Size: 532 5637
Bme1580I G_kGCm'C
Cuts at: 1790 2117 2479 3150 4230 5386 1790
Size: 327 362 671 1080 1156 2573
Fragments arranged by size:
2573 1156 1080 671 362 327
BmgBI CAC'GTC
Cuts at: 1318 2122 1318
Size: 804 5365
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2203 3900 4858
Size: 416 53 83 399 24 786 1697 958
Cuts at: 4858 442
Size: 1753
Fragments arranged by size:
1753 1697 958 786 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6169
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3285 3285
Size: 6169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2339 2559 2861 4829 1239
Size: 1100 220 302 1968 2579
Fragments arranged by size:
2579 1968 1100 302 220
Bpu10I CC'TnA_GC
Cuts at: 2201 2201
Size: 6169
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2317 2606 2873 4294 5159 5457 2317
Size: 289 267 1421 865 298 3029
Fragments arranged by size:
3029 1421 865 298 289 267
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3066 4832 882
Size: 617 1567 1766 2219
Fragments arranged by size:
2219 1766 1567 617
BsaAI yAC'GTr
Cuts at: 493 1204 2509 3436 3960 493
Size: 711 1305 927 524 2702
Fragments arranged by size:
2702 1305 927 711 524
BsaBI GATnn'nnATC
Cuts at: 2291 6163 2291
Size: 3872 2297
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2029 2103 2172 2643
Size: 53 83 186 936 496 74 69 471
Cuts at: 2643 3976 4358 4993 275
Size: 1333 382 635 1451
Fragments arranged by size:
1451 1333 936 635 496 471 382 186
83 74 69 53
BsaWI w'CCGG_w
Cuts at: 2823 4602 5000 5274 5421 5832 2823
Size: 1779 398 274 147 411 3160
Fragments arranged by size:
3160 1779 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2189 2219 1178
Size: 30 165 30 786 30 5128
Fragments arranged by size:
5128 786 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2105 1186
Size: 919 5250
BseYI C'CCAG_C
Cuts at: 2539 2871 3454 5372 5868 2539
Size: 332 583 1918 496 2840
Fragments arranged by size:
2840 1918 583 496 332
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2489 1163
Size: 573 753 4843
Fragments arranged by size:
4843 753 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2014 3619 4380 5408 1540
Size: 275 186 13 1605 761 1028 2301
Fragments arranged by size:
2301 1605 1028 761 275 186 13
BsiHKAI G_wGCw'C
Cuts at: 2799 3060 3150 3441 4230 4315 5386 2799
Size: 261 90 291 789 85 1071 3582
Fragments arranged by size:
3582 1071 789 291 261 90 85
BsmI GAATG_Cn'
Cuts at: 2444 3049 3680 3773 2444
Size: 605 631 93 4840
Fragments arranged by size:
4840 631 605 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2163 2163
Size: 6169
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2117 2479 2585 2799 3060 3150 3441
Size: 399 327 362 106 214 261 90 291
Cuts at: 3441 3488 4230 4315 5386 1391
Size: 47 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 362 327 291 261
214 106 90 85 47
BspEI T'CCGG_A
Cuts at: 2823 5832 2823
Size: 3009 3160
BspHI T'CATG_A
Cuts at: 4058 5788 4058
Size: 1730 4439
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3524 844
Size: 2680 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2811 4056 1394
Size: 429 988 1245 3507
Fragments arranged by size:
3507 1245 988 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2308 4664 4838 65
Size: 1183 1060 2356 174 1396
Fragments arranged by size:
2356 1396 1183 1060 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2174 4813 5000 1700
Size: 110 364 2639 187 2869
Fragments arranged by size:
2869 2639 364 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6169
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6169
BssSI C'ACGA_G
Cuts at: 3248 4229 5241 3248
Size: 981 1012 4176
Fragments arranged by size:
4176 1012 981
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6169
BstBI TT'CG_AA
Cuts at: 4973 4973
Size: 6169
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6169
BstXI CCAn_nnnn'nTGG
Cuts at: 2362 3327 2362
Size: 965 5204
BstYI r'GATC_y
Cuts at: 1972 2383 2847 3315 3499 3866 4251 4268 5720
Size: 411 464 468 184 367 385 17 1452
Cuts at: 5720 5806 5818 6164 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 468 464 411 385 367 346
184 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2014 2059 2130 2260 3048 513
Size: 552 939 10 45 71 130 788 3634
Fragments arranged by size:
3634 939 788 552 130 71 45 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2195 487
Size: 683 56 969 4461
Fragments arranged by size:
4461 969 683 56
BtsI GCAGTG_nn'
Cuts at: 2710 2903 3685 4479 4499 2710
Size: 193 782 794 20 4380
Fragments arranged by size:
4380 794 782 193 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6169
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2242 2277 529
Size: 35 1678 35 4421
Fragments arranged by size:
4421 1678 35 35
DraI TTT'AAA
Cuts at: 3473 3473
Size: 6169
DraIII CAC_nnn'GTG
Cuts at: 2725 2787 2725
Size: 62 6107
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5176 809
Size: 507 3860 1802
Fragments arranged by size:
3860 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2188 2421 2511
Size: 54 1425 50 275 18 358 233 90
Cuts at: 2511 2520 3210 3616 4505 6134 8
Size: 9 690 406 889 1629 43
Fragments arranged by size:
1629 1425 889 690 406 358 275 233
90 54 50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 3616 1537
Size: 275 1804 4090
Fragments arranged by size:
4090 1804 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2082 2621 2633 2777 3032 4099 1353
Size: 729 539 12 144 255 1067 3423
Fragments arranged by size:
3423 1067 729 539 255 144 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2099 3272 4756 5130 5276 387
Size: 1064 138 510 1173 1484 374 146 1280
Fragments arranged by size:
1484 1280 1173 1064 510 374 146 138
EcoICRI GAG'CTC
Cuts at: 2797 3058 2797
Size: 261 5908
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2233 2339 2399 2559 2861 2996 4232
Size: 393 601 106 60 160 302 135 1236
Cuts at: 4232 4829 5616 5884 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1236 787 601 597 393 302 268
160 135 106 60
EcoO109I rG'GnC_Cy
Cuts at: 2114 3451 2114
Size: 1337 4832
EcoRI G'AATT_C
Cuts at: 3478 3856 3478
Size: 378 5791
FspI TGC'GCA
Cuts at: 4675 4675
Size: 6169
HaeII r_GCGC'y
Cuts at: 1678 2032 2464 2597 2833 2971 5316 1678
Size: 354 432 133 236 138 2345 2531
Fragments arranged by size:
2531 2345 432 354 236 138 133
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2194 2226 2633 2665 4810
Size: 32 135 32 622 32 407 32 2145
Cuts at: 4810 4842 4884 4916 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2145 622 407 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2647 3513 3759 669
Size: 988 281 709 866 246 3079
Fragments arranged by size:
3079 988 866 709 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6169
HpaI GTT'AAC
Cuts at: 3759 3759
Size: 6169
KasI G'GCGC_C
Cuts at: 2028 2028
Size: 6169
KpnI G_GTAC'C
Cuts at: 3494 3494
Size: 6169
MfeI C'AATT_G
Cuts at: 3768 3768
Size: 6169
MluI A'CGCG_T
Cuts at: 6045 6045
Size: 6169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5283 5467 1305
Size: 3978 184 2007
Fragments arranged by size:
3978 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2513 2522 3212 10
Size: 54 1425 343 681 9 690 2967
Fragments arranged by size:
2967 1425 690 681 343 54 9
MslI CAynn'nnrTG
Cuts at: 518 1166 2345 2360 2576 3143 3302 4127 4486
Size: 648 1179 15 216 567 159 825 359
Cuts at: 4486 4645 518
Size: 159 2042
Fragments arranged by size:
2042 1179 825 648 567 359 216 159
159 15
MspA1I CmG'CkG
Cuts at: 1606 2016 2108 2132 2187 2543 3371 3458 3585
Size: 410 92 24 55 356 828 87 127
Cuts at: 3585 4262 5410 5655 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 828 677 410 356 245 127
92 87 55 24
NaeI GCC'GGC
Cuts at: 2176 2176
Size: 6169
NarI GG'CG_CC
Cuts at: 2029 2029
Size: 6169
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4678
Fragments arranged by size:
4678 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6169
NgoMIV G'CCGG_C
Cuts at: 2174 2174
Size: 6169
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6169
NotI GC'GGCC_GC
Cuts at: 3616 3616
Size: 6169
NspI r_CATG'y
Cuts at: 1132 1229 3527 5072 1132
Size: 97 2298 1545 2229
Fragments arranged by size:
2298 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6169
PciI A'CATG_T
Cuts at: 5068 5068
Size: 6169
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6169
PmeI GTTT'AAAC
Cuts at: 3473 3473
Size: 6169
PmlI CAC'GTG
Cuts at: 2509 2509
Size: 6169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2846 2878 4238 4270 5751 5783 2846
Size: 32 1360 32 1481 32 3232
Fragments arranged by size:
3232 1481 1360 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6169
PsiI TTA'TAA
Cuts at: 3739 3739
Size: 6169
PspOMI G'GGCC_C
Cuts at: 2113 2113
Size: 6169
PspXI vC'TCGA_Gb
Cuts at: 2053 2053
Size: 6169
PstI C_TGCA'G
Cuts at: 830 2220 2689 3521 830
Size: 1390 469 832 3478
Fragments arranged by size:
3478 1390 832 469
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6169
PvuII CAG'CTG
Cuts at: 3371 3371
Size: 6169
SacI G_AGCT'C
Cuts at: 2799 3060 2799
Size: 261 5908
SacII CC_GC'GG
Cuts at: 2017 2133 2017
Size: 116 6053
SalI G'TCGA_C
Cuts at: 1936 3511 1936
Size: 1575 4594
SapI GCTCTTCn'nnn_
Cuts at: 2633 3032 2633
Size: 399 5770
SbfI CC_TGCA'GG
Cuts at: 2220 3521 2220
Size: 1301 4868
ScaI AGT'ACT
Cuts at: 4417 6129 4417
Size: 1712 4457
SexAI A'CCwGG_T
Cuts at: 3255 3255
Size: 6169
SfcI C'TryA_G
Cuts at: 826 1045 2216 2389 2685 3517 4652 5333 5524
Size: 219 1171 173 296 832 1135 681 191
Cuts at: 5524 826
Size: 1471
Fragments arranged by size:
1471 1171 1135 832 681 296 219 191
173
SfiI GGCCn_nnn'nGGCC
Cuts at: 2519 2519
Size: 6169
SfoI GGC'GCC
Cuts at: 2030 2030
Size: 6169
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6169
SgrAI Cr'CCGG_yG
Cuts at: 1700 2174 1700
Size: 474 5695
SmaI CCC'GGG
Cuts at: 1872 3496 1872
Size: 1624 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2053 2296 2621 2852 4273 5174
Size: 197 926 110 243 325 231 1421 901
Cuts at: 5174 5436 820
Size: 262 1553
Fragments arranged by size:
1553 1421 926 901 325 262 243 231
197 110
SnaBI TAC'GTA
Cuts at: 493 3960 493
Size: 3467 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6169
SphI G_CATG'C
Cuts at: 1132 3527 1132
Size: 2395 3774
SspI AAT'ATT
Cuts at: 5 52 4093 5
Size: 47 4041 2081
Fragments arranged by size:
4041 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6169
StyI C'CwwG_G
Cuts at: 513 1065 2004 2673 3608 513
Size: 552 939 669 935 3074
Fragments arranged by size:
3074 939 935 669 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2462 2885 4196 4213 4366 4551 4996
Size: 1255 882 423 1311 17 153 185 445
Cuts at: 4996 325
Size: 1498
Fragments arranged by size:
1498 1311 1255 882 445 423 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4415 6127 96
Size: 274 80 33 51 1443 2438 1712 138
Fragments arranged by size:
2438 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2189 2221 6129 6161 2189
Size: 32 3908 32 2197
Fragments arranged by size:
3908 2197 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2041 1316
Size: 725 5444
XbaI T'CTAG_A
Cuts at: 3505 3505
Size: 6169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3356 3356
Size: 6169
XhoI C'TCGA_G
Cuts at: 1943 2053 1943
Size: 110 6059
XmaI C'CCGG_G
Cuts at: 1870 3494 1870
Size: 1624 4545
XmnI GAAnn'nnTTC
Cuts at: 2933 4298 2933
Size: 1365 4804
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2644 3977 276
Size: 53 83 186 936 1110 1333 2468
Fragments arranged by size:
2468 1333 1110 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
Bsu36I EcoNI EcoRV FalI FseI FspAI NruI NsiI
PacI PfoI PpuMI PsrI RsrII SanDI SrfI SwaI