Restriction map for
pFN21AE4465
(Circular) (Six-base) MAPSORT of: pFN21AE4465.seq Check: 6630 from: 1 to: 6166
pFN21AE4465 6166 bp
With 182 enzymes: SgfI *
March 28, 2012 13:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3976 278
Size: 53 83 186 936 2440 2468
Fragments arranged by size:
2468 2440 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3509 1937
Size: 1572 4594
Acc65I G'GTAC_C
Cuts at: 3487 3487
Size: 6166
AclI AA'CG_TT
Cuts at: 1563 4293 4666 1563
Size: 2730 373 3063
Fragments arranged by size:
3063 2730 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3407 4229 5613 5881 1632
Size: 1775 822 1384 268 1917
Fragments arranged by size:
1917 1775 1384 822 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5969
AflIII A'CryG_T
Cuts at: 1203 2817 5065 6042 1203
Size: 1614 2248 977 1327
Fragments arranged by size:
2248 1614 1327 977
AgeI A'CCGG_T
Cuts at: 4997 4997
Size: 6166
AhdI GACnn_n'nnGTC
Cuts at: 2076 4895 2076
Size: 2819 3347
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6166
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6166
AlwNI CAG_nnn'CTG
Cuts at: 3909 5481 3909
Size: 1572 4594
ApaI G_GGCC'C
Cuts at: 2196 2322 2196
Size: 126 6040
ApaLI G'TGCA_C
Cuts at: 4223 5379 4223
Size: 1156 5010
ApoI r'AATT_y
Cuts at: 1493 2620 3475 3701 3853 5962 1493
Size: 1127 855 226 152 2109 1697
Fragments arranged by size:
2109 1697 1127 855 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4720 160
Size: 872 3688 1606
Fragments arranged by size:
3688 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6166
AvaI C'yCGr_G
Cuts at: 1870 1943 3491 1870
Size: 73 1548 4545
Fragments arranged by size:
4545 1548 73
BamHI G'GATC_C
Cuts at: 2781 3496 3863 2781
Size: 715 367 5084
Fragments arranged by size:
5084 715 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2126 2240 2642 3045 3229
Size: 325 217 627 339 114 402 403 184
Cuts at: 3229 3487 4942 618
Size: 258 1455 1842
Fragments arranged by size:
1842 1455 627 403 402 339 325 258
217 184 114
BanII G_rGCy'C
Cuts at: 1391 2196 2322 3391 3485 1391
Size: 805 126 1069 94 4072
Fragments arranged by size:
4072 1069 805 126 94
BbeI G_GCGC'C
Cuts at: 2646 3049 2646
Size: 403 5763
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3462 928
Size: 971 1563 3632
Fragments arranged by size:
3632 1563 971
BbvCI CC'TCA_GC
Cuts at: 2393 2393
Size: 6166
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3397 3431 4357 4391 1903
Size: 34 1460 34 926 34 3678
Fragments arranged by size:
3678 1460 926 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2005 2304 4058 5268 2005
Size: 299 1754 1210 2903
Fragments arranged by size:
2903 1754 1210 299
BclI T'GATC_A
Cuts at: 1548 2271 1548
Size: 723 5443
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4777 136
Size: 107 122 71 631 605 3105 1525
Fragments arranged by size:
3105 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6161 6161
Size: 6166
BlpI GC'TnA_GC
Cuts at: 2171 3583 2171
Size: 1412 4754
Bme1580I G_kGCm'C
Cuts at: 1790 2129 2196 2245 2322 3234 4227 5383 1790
Size: 339 67 49 77 912 993 1156 2573
Fragments arranged by size:
2573 1156 993 912 339 77 67 49
BmgBI CAC'GTC
Cuts at: 1318 2373 1318
Size: 1055 5111
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3897 4855 442
Size: 416 53 83 399 24 2480 958 1753
Fragments arranged by size:
2480 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3039 4826 1239
Size: 1800 1787 2579
Fragments arranged by size:
2579 1800 1787
Bpu10I CC'TnA_GC
Cuts at: 2393 2393
Size: 6166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4291 5156 5454 4291
Size: 865 298 5003
Fragments arranged by size:
5003 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4829 882
Size: 617 3330 2219
Fragments arranged by size:
3330 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2992 3957 493
Size: 711 1788 965 2702
Fragments arranged by size:
2702 1788 965 711
BsaBI GATnn'nnATC
Cuts at: 3098 6160 3098
Size: 3062 3104
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2643 3046 3973 4355
Size: 53 83 186 936 1110 403 927 382
Cuts at: 4355 4990 275
Size: 635 1451
Fragments arranged by size:
1451 1110 936 927 635 403 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3448 4599 4997 5271 5418 5829 3448
Size: 1151 398 274 147 411 3785
Fragments arranged by size:
3785 1151 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2324 2354 1178
Size: 30 165 30 921 30 4990
Fragments arranged by size:
4990 921 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2433 1186
Size: 1247 4919
BseYI C'CCAG_C
Cuts at: 2092 2236 2518 2646 5369 5865 2092
Size: 144 282 128 2723 496 2393
Fragments arranged by size:
2723 2393 496 282 144 128
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5593
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3616 4377 5405 1540
Size: 275 186 1615 761 1028 2301
Fragments arranged by size:
2301 1615 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2172 2598 4227 4312 5383 2172
Size: 426 1629 85 1071 2955
Fragments arranged by size:
2955 1629 1071 426 85
BsmI GAATG_Cn'
Cuts at: 3677 3770 3677
Size: 93 6073
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2129 2172 2196 2245 2322 2598 3234
Size: 399 339 43 24 49 77 276 636
Cuts at: 3234 3391 3485 4227 4312 5383 1391
Size: 157 94 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 636 399 339 276 157
94 85 77 49 43 24
BspEI T'CCGG_A
Cuts at: 5829 5829
Size: 6166
BspHI T'CATG_A
Cuts at: 2811 4055 5785 2811
Size: 1244 1730 3192
Fragments arranged by size:
3192 1730 1244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2766 2972 3521 844
Size: 1922 206 549 3489
Fragments arranged by size:
3489 1922 549 206
BsrBI CCG'CTC
Cuts at: 1394 1823 4053 1394
Size: 429 2230 3507
Fragments arranged by size:
3507 2230 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4661 4835 65
Size: 1183 3413 174 1396
Fragments arranged by size:
3413 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4810 4997 1700
Size: 110 3000 187 2869
Fragments arranged by size:
3000 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6166
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6166
BssSI C'ACGA_G
Cuts at: 4226 5238 4226
Size: 1012 5154
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6166
BstBI TT'CG_AA
Cuts at: 4970 4970
Size: 6166
BstEII G'GTnAC_C
Cuts at: 1184 2885 1184
Size: 1701 4465
BstXI CCAn_nnnn'nTGG
Cuts at: 2122 2671 2122
Size: 549 5617
BstYI r'GATC_y
Cuts at: 1972 2145 2781 3496 3863 4248 4265 5717 5803
Size: 173 636 715 367 385 17 1452 86
Cuts at: 5803 5815 6161 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 715 636 385 367 346 173
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3462 3462
Size: 6166
BtgI C'CryG_G
Cuts at: 513 1065 2004 2547 513
Size: 552 939 543 4132
Fragments arranged by size:
4132 939 552 543
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5427
Fragments arranged by size:
5427 683 56
BtsI GCAGTG_nn'
Cuts at: 3682 4476 4496 3682
Size: 794 20 5352
Fragments arranged by size:
5352 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6166
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6131
DraI TTT'AAA
Cuts at: 3470 3470
Size: 6166
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5173 809
Size: 507 3857 1802
Fragments arranged by size:
3857 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2466 2550 2568
Size: 54 1425 50 275 18 636 84 18
Cuts at: 2568 2766 3613 4502 6131 8
Size: 198 847 889 1629 43
Fragments arranged by size:
1629 1425 889 847 636 275 198 84
54 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 3613 1537
Size: 275 1801 4090
Fragments arranged by size:
4090 1801 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2342 4096 1353
Size: 989 1754 3423
Fragments arranged by size:
3423 1754 989
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4753 5127 5273 387
Size: 1064 138 3164 374 146 1280
Fragments arranged by size:
3164 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3039 3407 4229 4826 5613 5881 1239
Size: 393 1407 368 822 597 787 268 1524
Fragments arranged by size:
1524 1407 822 787 597 393 368 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2306 2306
Size: 6166
EcoO109I rG'GnC_Cy
Cuts at: 2192 2193 2224 2961 2192
Size: 1 31 737 5397
Fragments arranged by size:
5397 737 31 1
EcoRI G'AATT_C
Cuts at: 3475 3853 3475
Size: 378 5788
FspI TGC'GCA
Cuts at: 4672 4672
Size: 6166
HaeII r_GCGC'y
Cuts at: 1678 2646 3049 5313 1678
Size: 968 403 2264 2531
Fragments arranged by size:
2531 2264 968 403
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2030 2062 2094 3104 3136
Size: 32 135 32 458 32 32 1010 32
Cuts at: 3136 4807 4839 4881 4913 1373
Size: 1671 32 42 32 2626
Fragments arranged by size:
2626 1671 1010 458 135 42 32 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3510 3756 669
Size: 988 281 1572 246 3079
Fragments arranged by size:
3079 1572 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6166
HpaI GTT'AAC
Cuts at: 3756 3756
Size: 6166
KasI G'GCGC_C
Cuts at: 2642 3045 2642
Size: 403 5763
KpnI G_GTAC'C
Cuts at: 3491 3491
Size: 6166
MfeI C'AATT_G
Cuts at: 3765 3765
Size: 6166
MluI A'CGCG_T
Cuts at: 6042 6042
Size: 6166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5280 5464 1305
Size: 3975 184 2007
Fragments arranged by size:
3975 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2570 2768 10
Size: 54 1425 343 738 198 3408
Fragments arranged by size:
3408 1425 738 343 198 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2120 2810 2816 2834 2990 3188 3266
Size: 648 954 690 6 18 156 198 78
Cuts at: 3266 4124 4483 4642 518
Size: 858 359 159 2042
Fragments arranged by size:
2042 954 858 690 648 359 198 159
156 78 18 6
MspA1I CmG'CkG
Cuts at: 1606 2549 2586 2650 2975 3582 4259 5407 5652
Size: 943 37 64 325 607 677 1148 245
Cuts at: 5652 1606
Size: 2120
Fragments arranged by size:
2120 1148 943 677 607 325 245 64
37
NarI GG'CG_CC
Cuts at: 2643 3046 2643
Size: 403 5763
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4675
Fragments arranged by size:
4675 939 552
NdeI CA'TA_TG
Cuts at: 387 2207 387
Size: 1820 4346
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6166
NotI GC'GGCC_GC
Cuts at: 3613 3613
Size: 6166
NruI TCG'CGA
Cuts at: 2231 2231
Size: 6166
NspI r_CATG'y
Cuts at: 1132 1229 3016 3524 5069 1132
Size: 97 1787 508 1545 2229
Fragments arranged by size:
2229 1787 1545 508 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6166
PciI A'CATG_T
Cuts at: 5065 5065
Size: 6166
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2869 1108
Size: 1761 4405
PmeI GTTT'AAAC
Cuts at: 3470 3470
Size: 6166
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4235 4267 5748 5780 4235
Size: 32 1481 32 4621
Fragments arranged by size:
4621 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2224 2961 2224
Size: 737 5429
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6166
PsiI TTA'TAA
Cuts at: 3736 3736
Size: 6166
PspOMI G'GGCC_C
Cuts at: 2192 2318 2192
Size: 126 6040
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2807 2839 2807
Size: 32 6134
PstI C_TGCA'G
Cuts at: 830 2859 3518 830
Size: 2029 659 3478
Fragments arranged by size:
3478 2029 659
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6166
PvuII CAG'CTG
Cuts at: 2650 2650
Size: 6166
SacII CC_GC'GG
Cuts at: 2550 2550
Size: 6166
SalI G'TCGA_C
Cuts at: 1936 3508 1936
Size: 1572 4594
SbfI CC_TGCA'GG
Cuts at: 3518 3518
Size: 6166
ScaI AGT'ACT
Cuts at: 4414 6126 4414
Size: 1712 4454
SfcI C'TryA_G
Cuts at: 826 1045 2855 3514 4649 5330 5521 826
Size: 219 1810 659 1135 681 191 1471
Fragments arranged by size:
1810 1471 1135 681 659 219 191
SfoI GGC'GCC
Cuts at: 2644 3047 2644
Size: 403 5763
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6166
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6166
SmaI CCC'GGG
Cuts at: 1872 3493 1872
Size: 1621 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4270 5171 5433 820
Size: 197 926 2327 901 262 1553
Fragments arranged by size:
2327 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3957 493
Size: 3464 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6166
SphI G_CATG'C
Cuts at: 1132 3524 1132
Size: 2392 3774
SspI AAT'ATT
Cuts at: 5 52 4090 5
Size: 47 4038 2081
Fragments arranged by size:
4038 2081 47
StuI AGG'CCT
Cuts at: 1517 2312 3220 1517
Size: 795 908 4463
Fragments arranged by size:
4463 908 795
StyI C'CwwG_G
Cuts at: 513 1065 2004 2605 3605 513
Size: 552 939 601 1000 3074
Fragments arranged by size:
3074 1000 939 601 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2228 4193 4210 4363 4548 4993 325
Size: 1255 648 1965 17 153 185 445 1498
Fragments arranged by size:
1965 1498 1255 648 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4412 6124 96
Size: 274 80 33 51 1443 2435 1712 138
Fragments arranged by size:
2435 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3171 3203 6126 6158 3171
Size: 32 2923 32 3179
Fragments arranged by size:
3179 2923 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6166
XbaI T'CTAG_A
Cuts at: 3502 3502
Size: 6166
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3377 3377
Size: 6166
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6166
XmaI C'CCGG_G
Cuts at: 1870 3491 1870
Size: 1621 4545
XmnI GAAnn'nnTTC
Cuts at: 2540 4295 2540
Size: 1755 4411
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3974 276
Size: 53 83 186 936 2440 2468
Fragments arranged by size:
2468 2440 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NruI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI NaeI NgoMIV NsiI PacI PfoI PmlI PspXI
RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI