Restriction map for
pFN21AE4456
(Circular) (Six-base) MAPSORT of: pFN21AE4456.seq Check: 9314 from: 1 to: 6130
pFN21AE4456 6130 bp
With 182 enzymes: SgfI *
March 28, 2012 13:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2483 2483
Size: 6130
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3940 278
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3473 1937
Size: 1536 4594
Acc65I G'GTAC_C
Cuts at: 2800 3451 2800
Size: 651 5479
AclI AA'CG_TT
Cuts at: 1563 4257 4630 1563
Size: 2694 373 3063
Fragments arranged by size:
3063 2694 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4193 5577 5845 1632
Size: 2561 1384 268 1917
Fragments arranged by size:
2561 1917 1384 268
AfeI AGC'GCT
Cuts at: 2256 2256
Size: 6130
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5933
AflIII A'CryG_T
Cuts at: 1203 5029 6006 1203
Size: 3826 977 1327
Fragments arranged by size:
3826 1327 977
AgeI A'CCGG_T
Cuts at: 4961 4961
Size: 6130
AhdI GACnn_n'nnGTC
Cuts at: 4859 4859
Size: 6130
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3417 1184
Size: 2233 3897
AlwNI CAG_nnn'CTG
Cuts at: 2593 3873 5445 2593
Size: 1280 1572 3278
Fragments arranged by size:
3278 1572 1280
ApaI G_GGCC'C
Cuts at: 2062 2062
Size: 6130
ApaLI G'TGCA_C
Cuts at: 4187 5343 4187
Size: 1156 4974
ApoI r'AATT_y
Cuts at: 1493 2609 2926 3137 3172 3439 3665 3817 5926
Size: 1116 317 211 35 267 226 152 2109
Cuts at: 5926 1493
Size: 1697
Fragments arranged by size:
2109 1697 1116 317 267 226 211 152
35
AseI AT'TA_AT
Cuts at: 160 1032 4684 160
Size: 872 3652 1606
Fragments arranged by size:
3652 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
AvaI C'yCGr_G
Cuts at: 1870 1943 3154 3455 1870
Size: 73 1211 301 4545
Fragments arranged by size:
4545 1211 301 73
BamHI G'GATC_C
Cuts at: 3043 3460 3827 3043
Size: 417 367 5346
Fragments arranged by size:
5346 417 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2800 3451 4906 618
Size: 325 217 627 1013 651 1455 1842
Fragments arranged by size:
1842 1455 1013 651 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2062 2640 3449 1391
Size: 671 578 809 4072
Fragments arranged by size:
4072 809 671 578
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2144 928
Size: 971 245 4914
Fragments arranged by size:
4914 971 245
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4321 4355 1903
Size: 34 2384 34 3678
Fragments arranged by size:
3678 2384 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4022 5232 4022
Size: 1210 4920
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6130
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2768 3363 4741
Size: 107 122 71 631 605 1096 595 1378
Cuts at: 4741 136
Size: 1525
Fragments arranged by size:
1525 1378 1096 631 605 595 122 107
71
BglII A'GATC_T
Cuts at: 6125 6125
Size: 6130
BlpI GC'TnA_GC
Cuts at: 3547 3547
Size: 6130
Bme1580I G_kGCm'C
Cuts at: 1790 2062 4191 5347 1790
Size: 272 2129 1156 2573
Fragments arranged by size:
2573 2129 1156 272
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6130
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3274 3861 4819
Size: 416 53 83 399 24 1857 587 958
Cuts at: 4819 442
Size: 1753
Fragments arranged by size:
1857 1753 958 587 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2885 3408 4790 1239
Size: 1646 523 1382 2579
Fragments arranged by size:
2579 1646 1382 523
Bpu10I CC'TnA_GC
Cuts at: 2634 2634
Size: 6130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2518 4255 5120 5418 2518
Size: 1737 865 298 3230
Fragments arranged by size:
3230 1737 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2663 4793 882
Size: 617 1164 2130 2219
Fragments arranged by size:
2219 2130 1164 617
BsaAI yAC'GTr
Cuts at: 493 1204 3921 493
Size: 711 2717 2702
Fragments arranged by size:
2717 2702 711
BsaBI GATnn'nnATC
Cuts at: 6124 6124
Size: 6130
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3937 4319 4954 275
Size: 53 83 186 936 2404 382 635 1451
Fragments arranged by size:
2404 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4563 4961 5235 5382 5793 4563
Size: 398 274 147 411 4900
Fragments arranged by size:
4900 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2622 2652 1178
Size: 30 165 30 1219 30 4656
Fragments arranged by size:
4656 1219 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6130
BseYI C'CCAG_C
Cuts at: 2097 2113 5333 5829 2097
Size: 16 3220 496 2398
Fragments arranged by size:
3220 2398 496 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3402 1163
Size: 573 1666 3891
Fragments arranged by size:
3891 1666 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3580 4341 5369 1540
Size: 275 186 1579 761 1028 2301
Fragments arranged by size:
2301 1579 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2640 4191 4276 5347 2640
Size: 1551 85 1071 3423
Fragments arranged by size:
3423 1551 1071 85
BsmI GAATG_Cn'
Cuts at: 2891 3641 3734 2891
Size: 750 93 5287
Fragments arranged by size:
5287 750 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2062 2640 3449 4191 4276 5347 1391
Size: 399 272 578 809 742 85 1071 2174
Fragments arranged by size:
2174 1071 809 742 578 399 272 85
BspEI T'CCGG_A
Cuts at: 5793 5793
Size: 6130
BspHI T'CATG_A
Cuts at: 3142 4019 5749 3142
Size: 877 1730 3523
Fragments arranged by size:
3523 1730 877
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2483 2896 3303 3485 844
Size: 1639 413 407 182 3489
Fragments arranged by size:
3489 1639 413 407 182
BsrBI CCG'CTC
Cuts at: 1394 1823 4017 1394
Size: 429 2194 3507
Fragments arranged by size:
3507 2194 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2353 2964 4625 4799 65
Size: 1183 1105 611 1661 174 1396
Fragments arranged by size:
1661 1396 1183 1105 611 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4774 4961 1700
Size: 110 2964 187 2869
Fragments arranged by size:
2964 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6130
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6130
BssSI C'ACGA_G
Cuts at: 4190 5202 4190
Size: 1012 5118
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6130
BstBI TT'CG_AA
Cuts at: 4934 4934
Size: 6130
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6130
BstXI CCAn_nnnn'nTGG
Cuts at: 2581 2581
Size: 6130
BstYI r'GATC_y
Cuts at: 1972 3043 3272 3460 3827 4212 4229 5681 5767
Size: 1071 229 188 367 385 17 1452 86
Cuts at: 5767 5779 6125 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1071 385 367 346 229 188
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2289 2289
Size: 6130
BtgI C'CryG_G
Cuts at: 513 1065 2412 2673 2991 3183 513
Size: 552 1347 261 318 192 3460
Fragments arranged by size:
3460 1347 552 318 261 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5391
Fragments arranged by size:
5391 683 56
BtsI GCAGTG_nn'
Cuts at: 2043 2741 3646 4440 4460 2043
Size: 698 905 794 20 3713
Fragments arranged by size:
3713 905 794 698 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2223 2258 2331 2366 2434 2469 529
Size: 35 1659 35 73 35 68 35 4190
Fragments arranged by size:
4190 1659 73 68 35 35 35 35
DraI TTT'AAA
Cuts at: 2032 3178 3434 2032
Size: 1146 256 4728
Fragments arranged by size:
4728 1146 256
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5137 809
Size: 507 3821 1802
Fragments arranged by size:
3821 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2470 2697 2874
Size: 54 1425 50 275 18 640 227 177
Cuts at: 2874 2988 3577 4466 6095 8
Size: 114 589 889 1629 43
Fragments arranged by size:
1629 1425 889 640 589 275 227 177
114 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3577 1537
Size: 275 1765 4090
Fragments arranged by size:
4090 1765 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4060 1353
Size: 2707 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2987 4717 5091 5237 387
Size: 1064 138 1398 1730 374 146 1280
Fragments arranged by size:
1730 1398 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2638 2638
Size: 6130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2885 3408 4193 4790 5577 5845 1239
Size: 393 1253 523 785 597 787 268 1524
Fragments arranged by size:
1524 1253 787 785 597 523 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2059 2059
Size: 6130
EcoRI G'AATT_C
Cuts at: 3439 3817 3439
Size: 378 5752
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2804 2804
Size: 6130
FspI TGC'GCA
Cuts at: 4636 4636
Size: 6130
HaeII r_GCGC'y
Cuts at: 1678 2258 5277 1678
Size: 580 3019 2531
Fragments arranged by size:
3019 2531 580
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2383 2415 2495 2527 2545
Size: 32 135 32 811 32 80 32 18
Cuts at: 2545 2577 2936 2968 4771 4803 4845 4877 1373
Size: 32 359 32 1803 32 42 32 2626
Fragments arranged by size:
2626 1803 811 359 135 80 42 32
32 32 32 32 32 32 32 18
HincII GTy'rAC
Cuts at: 669 1657 1938 3474 3720 669
Size: 988 281 1536 246 3079
Fragments arranged by size:
3079 1536 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3147 3417 748
Size: 2399 270 3461
Fragments arranged by size:
3461 2399 270
HpaI GTT'AAC
Cuts at: 3720 3720
Size: 6130
KpnI G_GTAC'C
Cuts at: 2804 3455 2804
Size: 651 5479
MfeI C'AATT_G
Cuts at: 3729 3729
Size: 6130
MluI A'CGCG_T
Cuts at: 6006 6006
Size: 6130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3214 5244 5428 1305
Size: 1909 2030 184 2007
Fragments arranged by size:
2030 2007 1909 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2472 2876 2990 10
Size: 54 1425 343 640 404 114 3150
Fragments arranged by size:
3150 1425 640 404 343 114 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2053 4088 4447 4606 518
Size: 648 887 2035 359 159 2042
Fragments arranged by size:
2042 2035 887 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2117 2702 2921 3386 3546 4223 5371 5616
Size: 511 585 219 465 160 677 1148 245
Cuts at: 5616 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 585 511 465 245 219
160
NcoI C'CATG_G
Cuts at: 513 1065 2412 2673 2991 3183 513
Size: 552 1347 261 318 192 3460
Fragments arranged by size:
3460 1347 552 318 261 192
NdeI CA'TA_TG
Cuts at: 387 3076 387
Size: 2689 3441
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6130
NotI GC'GGCC_GC
Cuts at: 3577 3577
Size: 6130
NspI r_CATG'y
Cuts at: 1132 1229 3488 5033 1132
Size: 97 2259 1545 2229
Fragments arranged by size:
2259 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3283 1348
Size: 1935 4195
PciI A'CATG_T
Cuts at: 5029 5029
Size: 6130
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6130
PmeI GTTT'AAAC
Cuts at: 3434 3434
Size: 6130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4199 4231 5712 5744 4199
Size: 32 1481 32 4585
Fragments arranged by size:
4585 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2282 1613
Size: 669 5461
PsiI TTA'TAA
Cuts at: 3000 3700 3000
Size: 700 5430
PspOMI G'GGCC_C
Cuts at: 2058 2058
Size: 6130
PstI C_TGCA'G
Cuts at: 830 2689 3314 3482 830
Size: 1859 625 168 3478
Fragments arranged by size:
3478 1859 625 168
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6130
PvuII CAG'CTG
Cuts at: 2117 2921 3386 2117
Size: 804 465 4861
Fragments arranged by size:
4861 804 465
SacI G_AGCT'C
Cuts at: 2640 2640
Size: 6130
SalI G'TCGA_C
Cuts at: 1936 3472 1936
Size: 1536 4594
SbfI CC_TGCA'GG
Cuts at: 3482 3482
Size: 6130
ScaI AGT'ACT
Cuts at: 4378 6090 4378
Size: 1712 4418
SfcI C'TryA_G
Cuts at: 826 1045 2685 3310 3478 4613 5294 5485 826
Size: 219 1640 625 168 1135 681 191 1471
Fragments arranged by size:
1640 1471 1135 681 625 219 191 168
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6130
SmaI CCC'GGG
Cuts at: 1872 3457 1872
Size: 1585 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2533 4234 5135 5397 820
Size: 197 926 590 1701 901 262 1553
Fragments arranged by size:
1701 1553 926 901 590 262 197
SnaBI TAC'GTA
Cuts at: 493 3921 493
Size: 3428 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6130
SphI G_CATG'C
Cuts at: 1132 3488 1132
Size: 2356 3774
SspI AAT'ATT
Cuts at: 5 52 4054 5
Size: 47 4002 2081
Fragments arranged by size:
4002 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6130
StyI C'CwwG_G
Cuts at: 513 1065 2368 2412 2673 2991 3183 3228 3569
Size: 552 1303 44 261 318 192 45 341
Cuts at: 3569 513
Size: 3074
Fragments arranged by size:
3074 1303 552 341 318 261 192 45
44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2706 3306 4157 4174 4327 4512 4957
Size: 1255 1126 600 851 17 153 185 445
Cuts at: 4957 325
Size: 1498
Fragments arranged by size:
1498 1255 1126 851 600 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4376 6088 96
Size: 274 80 33 51 1443 2399 1712 138
Fragments arranged by size:
2399 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2622 2654 6090 6122 2622
Size: 32 3436 32 2630
Fragments arranged by size:
3436 2630 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6130
XbaI T'CTAG_A
Cuts at: 3094 3466 3094
Size: 372 5758
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2671 2671
Size: 6130
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6130
XmaI C'CCGG_G
Cuts at: 1870 3455 1870
Size: 1585 4545
XmnI GAAnn'nnTTC
Cuts at: 4259 4259
Size: 6130
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3938 276
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI