Restriction map for
pFN21AE4454
(Circular) (Six-base) MAPSORT of: pFN21AE4454.seq Check: 3985 from: 1 to: 6127
pFN21AE4454 6127 bp
With 182 enzymes: SgfI *
March 28, 2012 13:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3937 278
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2833 2854 3470 1937
Size: 896 21 616 4594
Fragments arranged by size:
4594 896 616 21
Acc65I G'GTAC_C
Cuts at: 3448 3448
Size: 6127
AclI AA'CG_TT
Cuts at: 1563 4254 4627 1563
Size: 2691 373 3063
Fragments arranged by size:
3063 2691 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3182 4190 5574 5842 1632
Size: 1550 1008 1384 268 1917
Fragments arranged by size:
1917 1550 1384 1008 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5930
AflIII A'CryG_T
Cuts at: 1203 2388 2497 5026 6003 1203
Size: 1185 109 2529 977 1327
Fragments arranged by size:
2529 1327 1185 977 109
AgeI A'CCGG_T
Cuts at: 4958 4958
Size: 6127
AhdI GACnn_n'nnGTC
Cuts at: 4856 4856
Size: 6127
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6127
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2217 1184
Size: 1033 5094
AlwNI CAG_nnn'CTG
Cuts at: 3151 3870 5442 3151
Size: 719 1572 3836
Fragments arranged by size:
3836 1572 719
ApaI G_GGCC'C
Cuts at: 2083 2083
Size: 6127
ApaLI G'TGCA_C
Cuts at: 2489 4184 5340 2489
Size: 1695 1156 3276
Fragments arranged by size:
3276 1695 1156
ApoI r'AATT_y
Cuts at: 1493 3436 3662 3814 5923 1493
Size: 1943 226 152 2109 1697
Fragments arranged by size:
2109 1943 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4681 160
Size: 872 3649 1606
Fragments arranged by size:
3649 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
AvaI C'yCGr_G
Cuts at: 1870 1943 3255 3452 1870
Size: 73 1312 197 4545
Fragments arranged by size:
4545 1312 197 73
BamHI G'GATC_C
Cuts at: 3457 3824 3457
Size: 367 5760
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3448 4903 618
Size: 325 217 627 1661 1455 1842
Fragments arranged by size:
1842 1661 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2083 2121 2598 3446 1391
Size: 692 38 477 848 4072
Fragments arranged by size:
4072 848 692 477 38
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5156
BbvCI CC'TCA_GC
Cuts at: 2088 2102 2088
Size: 14 6113
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3050 3084 3332 3366 4318 4352 1903
Size: 34 1113 34 248 34 952 34 3678
Fragments arranged by size:
3678 1113 952 248 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4019 5229 4019
Size: 1210 4917
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6127
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4738 136
Size: 107 122 71 631 605 3066 1525
Fragments arranged by size:
3066 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6122 6122
Size: 6127
BlpI GC'TnA_GC
Cuts at: 2936 3544 2936
Size: 608 5519
Bme1580I G_kGCm'C
Cuts at: 1790 2083 2185 2493 2888 4188 5344 1790
Size: 293 102 308 395 1300 1156 2573
Fragments arranged by size:
2573 1300 1156 395 308 293 102
BmgBI CAC'GTC
Cuts at: 1318 2852 1318
Size: 1534 4593
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2026 2172 3858
Size: 416 53 83 399 24 609 146 1686
Cuts at: 3858 4816 442
Size: 958 1753
Fragments arranged by size:
1753 1686 958 609 416 399 146 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6127
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3199 3199
Size: 6127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2210 2282 2882 2975 4787 1239
Size: 971 72 600 93 1812 2579
Fragments arranged by size:
2579 1812 971 600 93 72
Bpu10I CC'TnA_GC
Cuts at: 2088 2102 3213 2088
Size: 14 1111 5002
Fragments arranged by size:
5002 1111 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2619 4252 5117 5415 2619
Size: 1633 865 298 3331
Fragments arranged by size:
3331 1633 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2748 4790 882
Size: 617 1249 2042 2219
Fragments arranged by size:
2219 2042 1249 617
BsaAI yAC'GTr
Cuts at: 493 1204 3337 3918 493
Size: 711 2133 581 2702
Fragments arranged by size:
2702 2133 711 581
BsaBI GATnn'nnATC
Cuts at: 2435 6121 2435
Size: 3686 2441
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3934 4316 4951 275
Size: 53 83 186 936 2401 382 635 1451
Fragments arranged by size:
2401 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4560 4958 5232 5379 5790 4560
Size: 398 274 147 411 4897
Fragments arranged by size:
4897 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3387 3417 1178
Size: 30 165 30 1984 30 3888
Fragments arranged by size:
3888 1984 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 3188 1186
Size: 880 1122 4125
Fragments arranged by size:
4125 1122 880
BseYI C'CCAG_C
Cuts at: 2416 2482 2555 2901 2909 3280 5330 5826 2416
Size: 66 73 346 8 371 2050 496 2717
Fragments arranged by size:
2717 2050 496 371 346 73 66 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2228 2945 1163
Size: 573 492 717 4345
Fragments arranged by size:
4345 717 573 492
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3577 4338 5366 1540
Size: 275 186 1576 761 1028 2301
Fragments arranged by size:
2301 1576 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2493 2598 4188 4273 5344 2493
Size: 105 1590 85 1071 3276
Fragments arranged by size:
3276 1590 1071 105 85
BsmI GAATG_Cn'
Cuts at: 3638 3731 3638
Size: 93 6034
BsmBI CGTCTCn'nnnn_
Cuts at: 2315 2315
Size: 6127
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2083 2121 2185 2493 2598 2888 3446
Size: 399 293 38 64 308 105 290 558
Cuts at: 3446 4188 4273 5344 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 558 399 308 293 290
105 85 64 38
BspEI T'CCGG_A
Cuts at: 5790 5790
Size: 6127
BspHI T'CATG_A
Cuts at: 4016 5746 4016
Size: 1730 4397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2232 3482 844
Size: 1388 1250 3489
Fragments arranged by size:
3489 1388 1250
BsrBI CCG'CTC
Cuts at: 1394 1823 2306 4014 1394
Size: 429 483 1708 3507
Fragments arranged by size:
3507 1708 483 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2346 2892 4622 4796 65
Size: 1183 1098 546 1730 174 1396
Fragments arranged by size:
1730 1396 1183 1098 546 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3081 4771 4958 1700
Size: 110 1271 1690 187 2869
Fragments arranged by size:
2869 1690 1271 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6127
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6127
BssSI C'ACGA_G
Cuts at: 3320 4187 5199 3320
Size: 867 1012 4248
Fragments arranged by size:
4248 1012 867
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6127
BstBI TT'CG_AA
Cuts at: 3352 4931 3352
Size: 1579 4548
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6127
BstXI CCAn_nnnn'nTGG
Cuts at: 2617 2905 2909 2617
Size: 288 4 5835
Fragments arranged by size:
5835 288 4
BstYI r'GATC_y
Cuts at: 1972 2538 3457 3824 4209 4226 5678 5764 5776
Size: 566 919 367 385 17 1452 86 12
Cuts at: 5776 6122 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 919 566 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2979 2979
Size: 6127
BtgI C'CryG_G
Cuts at: 513 1065 2004 2070 513
Size: 552 939 66 4570
Fragments arranged by size:
4570 939 552 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3040 487
Size: 683 56 1814 3574
Fragments arranged by size:
3574 1814 683 56
BtsI GCAGTG_nn'
Cuts at: 3643 4437 4457 3643
Size: 794 20 5313
Fragments arranged by size:
5313 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6127
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6092
DraI TTT'AAA
Cuts at: 3431 3431
Size: 6127
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5134 809
Size: 507 3818 1802
Fragments arranged by size:
3818 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2688 2776
Size: 54 1425 50 275 18 177 681 88
Cuts at: 2776 2971 3574 4463 6092 8
Size: 195 603 889 1629 43
Fragments arranged by size:
1629 1425 889 681 603 275 195 177
88 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3574 1537
Size: 275 1762 4090
Fragments arranged by size:
4090 1762 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2165 4057 1353
Size: 812 1892 3423
Fragments arranged by size:
3423 1892 812
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4714 5088 5234 387
Size: 1064 138 3125 374 146 1280
Fragments arranged by size:
3125 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2596 2596
Size: 6127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 2282 2882 2975 3182 4190 4787
Size: 393 578 72 600 93 207 1008 597
Cuts at: 4787 5574 5842 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1008 787 600 597 578 393 268
207 93 72
EcoNI CCTnn'n_nnAGG
Cuts at: 2030 2087 2339 2030
Size: 57 252 5818
Fragments arranged by size:
5818 252 57
EcoO109I rG'GnC_Cy
Cuts at: 2080 2106 2464 3064 3148 3180 2080
Size: 26 358 600 84 32 5027
Fragments arranged by size:
5027 600 358 84 32 26
EcoRI G'AATT_C
Cuts at: 3436 3814 3436
Size: 378 5749
EcoRV GAT'ATC
Cuts at: 2728 2728
Size: 6127
FspI TGC'GCA
Cuts at: 4633 4633
Size: 6127
HaeII r_GCGC'y
Cuts at: 1678 5274 1678
Size: 3596 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2487 2519 3387 3419 4768
Size: 32 135 32 915 32 868 32 1349
Cuts at: 4768 4800 4842 4874 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1349 915 868 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3471 3717 669
Size: 988 281 1533 246 3079
Fragments arranged by size:
3079 1533 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6127
HpaI GTT'AAC
Cuts at: 3717 3717
Size: 6127
KpnI G_GTAC'C
Cuts at: 3452 3452
Size: 6127
MfeI C'AATT_G
Cuts at: 3726 3726
Size: 6127
MluI A'CGCG_T
Cuts at: 6003 6003
Size: 6127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2734 5241 5425 1305
Size: 1429 2507 184 2007
Fragments arranged by size:
2507 2007 1429 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2690 2778 2973 10
Size: 54 1425 343 177 681 88 195 3164
Fragments arranged by size:
3164 1425 681 343 195 177 88 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2496 2702 3194 3375 4085 4444 4603
Size: 648 1330 206 492 181 710 359 159
Cuts at: 4603 518
Size: 2042
Fragments arranged by size:
2042 1330 710 648 492 359 206 181
159
MspA1I CmG'CkG
Cuts at: 1606 2486 2905 3160 3392 3543 4220 5368 5613
Size: 880 419 255 232 151 677 1148 245
Cuts at: 5613 1606
Size: 2120
Fragments arranged by size:
2120 1148 880 677 419 255 245 232
151
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4636
Fragments arranged by size:
4636 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6127
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6127
NotI GC'GGCC_GC
Cuts at: 3574 3574
Size: 6127
NspI r_CATG'y
Cuts at: 1132 1229 2392 2501 2818 2876 2953 3374 3485
Size: 97 1163 109 317 58 77 421 111
Cuts at: 3485 5030 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1163 421 317 111 109 97
77 58
PasI CC'CwG_GG
Cuts at: 1348 2572 1348
Size: 1224 4903
PciI A'CATG_T
Cuts at: 2388 2497 5026 2388
Size: 109 2529 3489
Fragments arranged by size:
3489 2529 109
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2016 2589 1108
Size: 908 573 4646
Fragments arranged by size:
4646 908 573
PfoI T'CCnGG_A
Cuts at: 2259 2474 2802 3405 2259
Size: 215 328 603 4981
Fragments arranged by size:
4981 603 328 215
PmeI GTTT'AAAC
Cuts at: 3431 3431
Size: 6127
PmlI CAC'GTG
Cuts at: 3337 3337
Size: 6127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4196 4228 5709 5741 4196
Size: 32 1481 32 4582
Fragments arranged by size:
4582 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2106 2106
Size: 6127
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6127
PsiI TTA'TAA
Cuts at: 3697 3697
Size: 6127
PspOMI G'GGCC_C
Cuts at: 2079 2079
Size: 6127
PstI C_TGCA'G
Cuts at: 830 3479 830
Size: 2649 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6127
PvuII CAG'CTG
Cuts at: 2486 2905 3160 2486
Size: 419 255 5453
Fragments arranged by size:
5453 419 255
SacI G_AGCT'C
Cuts at: 2598 2598
Size: 6127
SalI G'TCGA_C
Cuts at: 1936 3469 1936
Size: 1533 4594
SapI GCTCTTCn'nnn_
Cuts at: 2165 2165
Size: 6127
SbfI CC_TGCA'GG
Cuts at: 3479 3479
Size: 6127
ScaI AGT'ACT
Cuts at: 4375 6087 4375
Size: 1712 4415
SfcI C'TryA_G
Cuts at: 826 1045 2834 3475 4610 5291 5482 826
Size: 219 1789 641 1135 681 191 1471
Fragments arranged by size:
1789 1471 1135 681 641 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6127
SmaI CCC'GGG
Cuts at: 1872 3454 1872
Size: 1582 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2634 4231 5132 5394 820
Size: 197 926 691 1597 901 262 1553
Fragments arranged by size:
1597 1553 926 901 691 262 197
SnaBI TAC'GTA
Cuts at: 493 3918 493
Size: 3425 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6127
SphI G_CATG'C
Cuts at: 1132 2953 3374 3485 1132
Size: 1821 421 111 3774
Fragments arranged by size:
3774 1821 421 111
SspI AAT'ATT
Cuts at: 5 52 4051 5
Size: 47 3999 2081
Fragments arranged by size:
3999 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6127
StyI C'CwwG_G
Cuts at: 513 1065 2004 3566 513
Size: 552 939 1562 3074
Fragments arranged by size:
3074 1562 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2623 2772 2915 3023 4154 4171 4324
Size: 1255 1043 149 143 108 1131 17 153
Cuts at: 4324 4509 4954 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1131 1043 445 185 153 149
143 108 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2826 4373 6085
Size: 274 80 33 51 1443 849 1547 1712
Cuts at: 6085 96
Size: 138
Fragments arranged by size:
1712 1547 1443 849 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6087 6119 6087
Size: 32 6095
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6127
XbaI T'CTAG_A
Cuts at: 3463 3463
Size: 6127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2185 2563 3107 2185
Size: 378 544 5205
Fragments arranged by size:
5205 544 378
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6127
XmaI C'CCGG_G
Cuts at: 1870 3452 1870
Size: 1582 4545
XmnI GAAnn'nnTTC
Cuts at: 4256 4256
Size: 6127
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3935 276
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I DraIII FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI