Restriction map for
pFN21AE4453
(Circular) (Six-base) MAPSORT of: pFN21AE4453.seq Check: 9098 from: 1 to: 6124
pFN21AE4453 6124 bp
With 182 enzymes: SgfI *
March 28, 2012 13:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2021 2021
Size: 6124
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2664 3934 278
Size: 53 83 186 936 1128 1270 2468
Fragments arranged by size:
2468 1270 1128 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3467 1937
Size: 1530 4594
Acc65I G'GTAC_C
Cuts at: 2460 3445 2460
Size: 985 5139
AclI AA'CG_TT
Cuts at: 1563 4251 4624 1563
Size: 2688 373 3063
Fragments arranged by size:
3063 2688 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2203 2593 2756 3388 4187 5571 5839 1632
Size: 571 390 163 632 799 1384 268 1917
Fragments arranged by size:
1917 1384 799 632 571 390 268 163
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5927
AflIII A'CryG_T
Cuts at: 1203 5023 6000 1203
Size: 3820 977 1327
Fragments arranged by size:
3820 1327 977
AgeI A'CCGG_T
Cuts at: 4955 4955
Size: 6124
AhdI GACnn_n'nnGTC
Cuts at: 4853 4853
Size: 6124
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6124
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3261 1184
Size: 2077 4047
AlwNI CAG_nnn'CTG
Cuts at: 3031 3136 3867 5439 3031
Size: 105 731 1572 3716
Fragments arranged by size:
3716 1572 731 105
ApaLI G'TGCA_C
Cuts at: 4181 5337 4181
Size: 1156 4968
ApoI r'AATT_y
Cuts at: 1493 3433 3659 3811 5920 1493
Size: 1940 226 152 2109 1697
Fragments arranged by size:
2109 1940 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4678 160
Size: 872 3646 1606
Fragments arranged by size:
3646 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
AvaI C'yCGr_G
Cuts at: 1870 1943 3449 1870
Size: 73 1506 4545
Fragments arranged by size:
4545 1506 73
BamHI G'GATC_C
Cuts at: 3454 3821 3454
Size: 367 5757
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2460 3445 4900 618
Size: 325 217 627 673 985 1455 1842
Fragments arranged by size:
1842 1455 985 673 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2054 3012 3395 3422 3443 1391
Size: 663 958 383 27 21 4072
Fragments arranged by size:
4072 958 663 383 27 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3230 3325 928
Size: 971 1331 95 3727
Fragments arranged by size:
3727 1331 971 95
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4315 4349 1903
Size: 34 2378 34 3678
Fragments arranged by size:
3678 2378 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4016 5226 4016
Size: 1210 4914
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6124
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2594 4735 136
Size: 107 122 71 631 605 922 2141 1525
Fragments arranged by size:
2141 1525 922 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3207 6119 3207
Size: 2912 3212
BlpI GC'TnA_GC
Cuts at: 3056 3541 3056
Size: 485 5639
Bme1580I G_kGCm'C
Cuts at: 1790 2131 4185 5341 1790
Size: 341 2054 1156 2573
Fragments arranged by size:
2573 2054 1156 341
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6124
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2472 3855 4813
Size: 416 53 83 399 24 1055 1383 958
Cuts at: 4813 442
Size: 1753
Fragments arranged by size:
1753 1383 1055 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6124
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3409 3409
Size: 6124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2020 2071 2438 2735 3008 3053 3095 3107
Size: 781 51 367 297 273 45 42 12
Cuts at: 3107 3254 3287 4784 1239
Size: 147 33 1497 2579
Fragments arranged by size:
2579 1497 781 367 297 273 147 51
45 42 33 12
Bpu10I CC'TnA_GC
Cuts at: 2849 2930 2849
Size: 81 6043
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3365 4249 5114 5412 3365
Size: 884 865 298 4077
Fragments arranged by size:
4077 884 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2912 3086 4787 882
Size: 617 1413 174 1701 2219
Fragments arranged by size:
2219 1701 1413 617 174
BsaAI yAC'GTr
Cuts at: 493 1204 3915 493
Size: 711 2711 2702
Fragments arranged by size:
2711 2702 711
BsaBI GATnn'nnATC
Cuts at: 2723 6118 2723
Size: 3395 2729
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2661 3931 4313 4948
Size: 53 83 186 936 1128 1270 382 635
Cuts at: 4948 275
Size: 1451
Fragments arranged by size:
1451 1270 1128 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3012 4557 4955 5229 5376 5787 3012
Size: 1545 398 274 147 411 3349
Fragments arranged by size:
3349 1545 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3275 3305 1178
Size: 30 165 30 1872 30 3997
Fragments arranged by size:
3997 1872 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2023 2089 2152 2164 2435 2756 2786 2789
Size: 837 66 63 12 271 321 30 3
Cuts at: 2789 2957 3104 3167 3331 3334 1186
Size: 168 147 63 164 3 3976
Fragments arranged by size:
3976 837 321 271 168 164 147 66
63 63 30 12 3 3
BseYI C'CCAG_C
Cuts at: 2132 2194 3051 3165 3294 5327 5823 2132
Size: 62 857 114 129 2033 496 2433
Fragments arranged by size:
2433 2033 857 496 129 114 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2817 1163
Size: 573 1081 4470
Fragments arranged by size:
4470 1081 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3574 4335 5363 1540
Size: 275 186 1573 761 1028 2301
Fragments arranged by size:
2301 1573 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2416 3012 3024 3395 3422 4185 4270 5341 2416
Size: 596 12 371 27 763 85 1071 3199
Fragments arranged by size:
3199 1071 763 596 371 85 27 12
BsmI GAATG_Cn'
Cuts at: 2563 3635 3728 2563
Size: 1072 93 4959
Fragments arranged by size:
4959 1072 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2904 2904
Size: 6124
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2131 2416 3012 3024 3395 3422
Size: 399 264 77 285 596 12 371 27
Cuts at: 3422 3443 4185 4270 5341 1391
Size: 21 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 596 399 371 285 264
85 77 27 21 12
BspEI T'CCGG_A
Cuts at: 3012 5787 3012
Size: 2775 3349
BspHI T'CATG_A
Cuts at: 4013 5743 4013
Size: 1730 4394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2021 3479 844
Size: 1177 1458 3489
Fragments arranged by size:
3489 1458 1177
BsrBI CCG'CTC
Cuts at: 1394 1823 4011 1394
Size: 429 2188 3507
Fragments arranged by size:
3507 2188 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2538 4619 4793 65
Size: 1183 1290 2081 174 1396
Fragments arranged by size:
2081 1396 1290 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2801 4768 4955 1700
Size: 110 991 1967 187 2869
Fragments arranged by size:
2869 1967 991 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6124
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6124
BssSI C'ACGA_G
Cuts at: 2768 4184 5196 2768
Size: 1416 1012 3696
Fragments arranged by size:
3696 1416 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6124
BstBI TT'CG_AA
Cuts at: 4928 4928
Size: 6124
BstEII G'GTnAC_C
Cuts at: 1184 2359 1184
Size: 1175 4949
BstYI r'GATC_y
Cuts at: 1972 2448 3126 3207 3454 3821 4206 4223 5675
Size: 476 678 81 247 367 385 17 1452
Cuts at: 5675 5761 5773 6119 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 678 476 385 367 346 247
86 81 17 12
Bsu36I CC'TnA_GG
Cuts at: 2525 2756 2525
Size: 231 5893
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5572
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5385
Fragments arranged by size:
5385 683 56
BtsI GCAGTG_nn'
Cuts at: 2356 3001 3151 3640 4434 4454 2356
Size: 645 150 489 794 20 4026
Fragments arranged by size:
4026 794 645 489 150 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6124
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6089
DraI TTT'AAA
Cuts at: 3428 3428
Size: 6124
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5131 809
Size: 507 3815 1802
Fragments arranged by size:
3815 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2109 2544 2700
Size: 54 1425 50 275 18 279 435 156
Cuts at: 2700 3307 3571 4460 6089 8
Size: 607 264 889 1629 43
Fragments arranged by size:
1629 1425 889 607 435 279 275 264
156 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3571 1537
Size: 275 1759 4090
Fragments arranged by size:
4090 1759 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3358 4054 1353
Size: 2005 696 3423
Fragments arranged by size:
3423 2005 696
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2820 4711 5085 5231 387
Size: 1064 138 1231 1891 374 146 1280
Fragments arranged by size:
1891 1280 1231 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3010 3393 3420 3010
Size: 383 27 5714
Fragments arranged by size:
5714 383 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2020 2071 2203 2438 2593 2735 2756
Size: 393 388 51 132 235 155 142 21
Cuts at: 2756 3008 3053 3095 3107 3254 3287 3388 4187
Size: 252 45 42 12 147 33 101 799
Cuts at: 4187 4784 5571 5839 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 799 787 597 393 388 268 252
235 155 147 142 132 101 51 45
42 33 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2402 2738 2755 3266 2402
Size: 336 17 511 5260
Fragments arranged by size:
5260 511 336 17
EcoO109I rG'GnC_Cy
Cuts at: 2118 2521 3028 2118
Size: 403 507 5214
Fragments arranged by size:
5214 507 403
EcoRI G'AATT_C
Cuts at: 3433 3811 3433
Size: 378 5746
FspI TGC'GCA
Cuts at: 2562 4630 2562
Size: 2068 4056
HaeII r_GCGC'y
Cuts at: 1678 5271 1678
Size: 3593 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2058 2090 2770 2802 3032
Size: 32 135 32 486 32 680 32 230
Cuts at: 3032 3064 4765 4797 4839 4871 1373
Size: 32 1701 32 42 32 2626
Fragments arranged by size:
2626 1701 680 486 230 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3468 3714 669
Size: 988 281 1530 246 3079
Fragments arranged by size:
3079 1530 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6124
HpaI GTT'AAC
Cuts at: 3714 3714
Size: 6124
KpnI G_GTAC'C
Cuts at: 2464 3449 2464
Size: 985 5139
MfeI C'AATT_G
Cuts at: 3723 3723
Size: 6124
MluI A'CGCG_T
Cuts at: 6000 6000
Size: 6124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2664 2991 5238 5422 1305
Size: 1359 327 2247 184 2007
Fragments arranged by size:
2247 2007 1359 327 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2702 3309 10
Size: 54 1425 343 870 607 2825
Fragments arranged by size:
2825 1425 870 607 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4082 4441 4600 518
Size: 648 2916 359 159 2042
Fragments arranged by size:
2916 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2473 3118 3160 3540 4217 5365 5610 1606
Size: 867 645 42 380 677 1148 245 2120
Fragments arranged by size:
2120 1148 867 677 645 380 245 42
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5572
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6124
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6124
NotI GC'GGCC_GC
Cuts at: 3571 3571
Size: 6124
NspI r_CATG'y
Cuts at: 1132 1229 3115 3482 5027 1132
Size: 97 1886 367 1545 2229
Fragments arranged by size:
2229 1886 1545 367 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6124
PciI A'CATG_T
Cuts at: 5023 5023
Size: 6124
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3031 1108
Size: 1923 4201
PmeI GTTT'AAAC
Cuts at: 3428 3428
Size: 6124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2909 2941 4193 4225 5706 5738 2909
Size: 32 1252 32 1481 32 3295
Fragments arranged by size:
3295 1481 1252 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2118 2521 2118
Size: 403 5721
PshAI GACnn'nnGTC
Cuts at: 1613 2496 1613
Size: 883 5241
PsiI TTA'TAA
Cuts at: 3694 3694
Size: 6124
PstI C_TGCA'G
Cuts at: 830 2018 3048 3476 830
Size: 1188 1030 428 3478
Fragments arranged by size:
3478 1188 1030 428
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6124
PvuII CAG'CTG
Cuts at: 3118 3160 3118
Size: 42 6082
SacI G_AGCT'C
Cuts at: 3012 3395 3422 3012
Size: 383 27 5714
Fragments arranged by size:
5714 383 27
SalI G'TCGA_C
Cuts at: 1936 3466 1936
Size: 1530 4594
SbfI CC_TGCA'GG
Cuts at: 3476 3476
Size: 6124
ScaI AGT'ACT
Cuts at: 4372 6084 4372
Size: 1712 4412
SexAI A'CCwGG_T
Cuts at: 2921 2921
Size: 6124
SfcI C'TryA_G
Cuts at: 826 1045 2014 2494 3044 3472 4607 5288 5479
Size: 219 969 480 550 428 1135 681 191
Cuts at: 5479 826
Size: 1471
Fragments arranged by size:
1471 1135 969 681 550 480 428 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6124
SmaI CCC'GGG
Cuts at: 1872 3451 1872
Size: 1579 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3380 4228 5129 5391 820
Size: 197 926 1437 848 901 262 1553
Fragments arranged by size:
1553 1437 926 901 848 262 197
SnaBI TAC'GTA
Cuts at: 493 3915 493
Size: 3422 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6124
SphI G_CATG'C
Cuts at: 1132 3115 3482 1132
Size: 1983 367 3774
Fragments arranged by size:
3774 1983 367
SspI AAT'ATT
Cuts at: 5 52 4048 5
Size: 47 3996 2081
Fragments arranged by size:
3996 2081 47
StuI AGG'CCT
Cuts at: 1517 2733 1517
Size: 1216 4908
StyI C'CwwG_G
Cuts at: 513 1065 2272 3563 513
Size: 552 1207 1291 3074
Fragments arranged by size:
3074 1291 1207 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2288 2954 4151 4168 4321 4506 4951
Size: 1255 708 666 1197 17 153 185 445
Cuts at: 4951 325
Size: 1498
Fragments arranged by size:
1498 1255 1197 708 666 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4370 6082 96
Size: 274 80 33 51 1443 2393 1712 138
Fragments arranged by size:
2393 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3275 3307 6084 6116 3275
Size: 32 2777 32 3283
Fragments arranged by size:
3283 2777 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6124
XbaI T'CTAG_A
Cuts at: 3460 3460
Size: 6124
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2434 2710 2974 3137 3302 2434
Size: 276 264 163 165 5256
Fragments arranged by size:
5256 276 264 165 163
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6124
XmaI C'CCGG_G
Cuts at: 1870 3449 1870
Size: 1579 4545
XmnI GAAnn'nnTTC
Cuts at: 4253 4253
Size: 6124
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2662 3932 276
Size: 53 83 186 936 1128 1270 2468
Fragments arranged by size:
2468 1270 1128 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstXI BstZ17I DraIII EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SfiI SfoI SrfI SwaI