Restriction map for
pFN21AE4450
(Circular) (Six-base) MAPSORT of: pFN21AE4450.seq Check: 7599 from: 1 to: 6109
pFN21AE4450 6109 bp
With 182 enzymes: SgfI *
March 28, 2012 13:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2809 2809
Size: 6109
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3919 278
Size: 53 83 186 936 2383 2468
Fragments arranged by size:
2468 2383 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2424 3452 1937
Size: 487 1028 4594
Fragments arranged by size:
4594 1028 487
Acc65I G'GTAC_C
Cuts at: 3430 3430
Size: 6109
AclI AA'CG_TT
Cuts at: 1563 4236 4609 1563
Size: 2673 373 3063
Fragments arranged by size:
3063 2673 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2721 4172 5556 5824 1632
Size: 1089 1451 1384 268 1917
Fragments arranged by size:
1917 1451 1384 1089 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5912
AflIII A'CryG_T
Cuts at: 1203 5008 5985 1203
Size: 3805 977 1327
Fragments arranged by size:
3805 1327 977
AgeI A'CCGG_T
Cuts at: 4940 4940
Size: 6109
AhdI GACnn_n'nnGTC
Cuts at: 4838 4838
Size: 6109
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6109
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6109
AlwNI CAG_nnn'CTG
Cuts at: 2921 3852 5424 2921
Size: 931 1572 3606
Fragments arranged by size:
3606 1572 931
ApaI G_GGCC'C
Cuts at: 2283 2283
Size: 6109
ApaLI G'TGCA_C
Cuts at: 4166 5322 4166
Size: 1156 4953
ApoI r'AATT_y
Cuts at: 1493 3418 3644 3796 5905 1493
Size: 1925 226 152 2109 1697
Fragments arranged by size:
2109 1925 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4663 160
Size: 872 3631 1606
Fragments arranged by size:
3631 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
AvaI C'yCGr_G
Cuts at: 1870 1943 3434 1870
Size: 73 1491 4545
Fragments arranged by size:
4545 1491 73
BamHI G'GATC_C
Cuts at: 2888 3439 3806 2888
Size: 551 367 5191
Fragments arranged by size:
5191 551 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2010 3430 4885 618
Size: 325 217 627 223 1420 1455 1842
Fragments arranged by size:
1842 1455 1420 627 325 223 217
BanII G_rGCy'C
Cuts at: 1391 2283 2856 3428 1391
Size: 892 573 572 4072
Fragments arranged by size:
4072 892 573 572
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3190 3377 928
Size: 971 1291 187 3660
Fragments arranged by size:
3660 1291 971 187
BbvCI CC'TCA_GC
Cuts at: 2768 3146 2768
Size: 378 5731
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4300 4334 1903
Size: 34 2363 34 3678
Fragments arranged by size:
3678 2363 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4001 5211 4001
Size: 1210 4899
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6109
BfrBI ATG'CAT
Cuts at: 2318 2318
Size: 6109
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4720 136
Size: 107 122 71 631 605 3048 1525
Fragments arranged by size:
3048 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2386 6104 2386
Size: 3718 2391
BlpI GC'TnA_GC
Cuts at: 3526 3526
Size: 6109
Bme1580I G_kGCm'C
Cuts at: 1790 2013 2283 4170 5326 1790
Size: 223 270 1887 1156 2573
Fragments arranged by size:
2573 1887 1156 270 223
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6109
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2657 2681 3840
Size: 416 53 83 399 24 1240 24 1159
Cuts at: 3840 4798 442
Size: 958 1753
Fragments arranged by size:
1753 1240 1159 958 416 399 83 53
24 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2437 4769 1239
Size: 1198 2332 2579
Fragments arranged by size:
2579 2332 1198
Bpu10I CC'TnA_GC
Cuts at: 2768 3146 2768
Size: 378 5731
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4234 5099 5397 4234
Size: 865 298 4946
Fragments arranged by size:
4946 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4772 882
Size: 617 3273 2219
Fragments arranged by size:
3273 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3900 493
Size: 711 2696 2702
Fragments arranged by size:
2702 2696 711
BsaBI GATnn'nnATC
Cuts at: 6103 6103
Size: 6109
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3916 4298 4933 275
Size: 53 83 186 936 2383 382 635 1451
Fragments arranged by size:
2383 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4542 4940 5214 5361 5772 4542
Size: 398 274 147 411 4879
Fragments arranged by size:
4879 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5884
Fragments arranged by size:
5884 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6109
BseYI C'CCAG_C
Cuts at: 2106 2181 3176 5312 5808 2106
Size: 75 995 2136 496 2407
Fragments arranged by size:
2407 2136 995 496 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2787 1163
Size: 573 1051 4485
Fragments arranged by size:
4485 1051 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2296 3559 4320 5348 1540
Size: 275 186 295 1263 761 1028 2301
Fragments arranged by size:
2301 1263 1028 761 295 275 186
BsiHKAI G_wGCw'C
Cuts at: 2856 4170 4255 5326 2856
Size: 1314 85 1071 3639
Fragments arranged by size:
3639 1314 1071 85
BsmI GAATG_Cn'
Cuts at: 3620 3713 3620
Size: 93 6016
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2283 2856 3428 4170 4255 5326
Size: 399 223 270 573 572 742 85 1071
Cuts at: 5326 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 573 572 399 270 223
85
BspEI T'CCGG_A
Cuts at: 5772 5772
Size: 6109
BspHI T'CATG_A
Cuts at: 3998 5728 3998
Size: 1730 4379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2809 2906 3053 3464 844
Size: 1965 97 147 411 3489
Fragments arranged by size:
3489 1965 411 147 97
BsrBI CCG'CTC
Cuts at: 1394 1823 3996 1394
Size: 429 2173 3507
Fragments arranged by size:
3507 2173 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3291 4604 4778 65
Size: 1183 2043 1313 174 1396
Fragments arranged by size:
2043 1396 1313 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3374 4753 4940 1700
Size: 110 1564 1379 187 2869
Fragments arranged by size:
2869 1564 1379 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6109
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6109
BssSI C'ACGA_G
Cuts at: 4169 5181 4169
Size: 1012 5097
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6109
BstBI TT'CG_AA
Cuts at: 4913 4913
Size: 6109
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6109
BstXI CCAn_nnnn'nTGG
Cuts at: 2473 2473
Size: 6109
BstYI r'GATC_y
Cuts at: 1972 2386 2888 3439 3806 4191 4208 5660 5746
Size: 414 502 551 367 385 17 1452 86
Cuts at: 5746 5758 6104 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 551 502 414 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2425 2425
Size: 6109
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4618
Fragments arranged by size:
4618 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5370
Fragments arranged by size:
5370 683 56
BtsI GCAGTG_nn'
Cuts at: 2100 2686 3625 4419 4439 2100
Size: 586 939 794 20 3770
Fragments arranged by size:
3770 939 794 586 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6109
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6074
DraI TTT'AAA
Cuts at: 3413 3413
Size: 6109
DraIII CAC_nnn'GTG
Cuts at: 2035 2035
Size: 6109
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5116 809
Size: 507 3800 1802
Fragments arranged by size:
3800 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3556 4445 6074
Size: 54 1425 50 275 18 1726 889 1629
Cuts at: 6074 8
Size: 43
Fragments arranged by size:
1726 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3556 1537
Size: 275 1744 4090
Fragments arranged by size:
4090 1744 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4039 1353
Size: 2686 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4696 5070 5216 387
Size: 1064 138 3107 374 146 1280
Fragments arranged by size:
3107 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2854 2854
Size: 6109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2437 2721 4172 4769 5556 5824 1239
Size: 393 805 284 1451 597 787 268 1524
Fragments arranged by size:
1524 1451 805 787 597 393 284 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3112 3112
Size: 6109
EcoO109I rG'GnC_Cy
Cuts at: 2279 2598 2636 2279
Size: 319 38 5752
Fragments arranged by size:
5752 319 38
EcoRI G'AATT_C
Cuts at: 3418 3796 3418
Size: 378 5731
FspI TGC'GCA
Cuts at: 4615 4615
Size: 6109
HaeII r_GCGC'y
Cuts at: 1678 5256 1678
Size: 3578 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2533 2565 2590 2622 3014
Size: 32 135 32 961 32 25 32 392
Cuts at: 3014 3046 4750 4782 4824 4856 1373
Size: 32 1704 32 42 32 2626
Fragments arranged by size:
2626 1704 961 392 135 42 32 32
32 32 32 32 32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 3453 3699 669
Size: 988 281 1515 246 3079
Fragments arranged by size:
3079 1515 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6109
HpaI GTT'AAC
Cuts at: 3699 3699
Size: 6109
KpnI G_GTAC'C
Cuts at: 3434 3434
Size: 6109
MfeI C'AATT_G
Cuts at: 3708 3708
Size: 6109
MluI A'CGCG_T
Cuts at: 5985 5985
Size: 6109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2490 5223 5407 1305
Size: 1185 2733 184 2007
Fragments arranged by size:
2733 2007 1185 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4287
Fragments arranged by size:
4287 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2873 3389 4067 4426 4585 518
Size: 648 1707 516 678 359 159 2042
Fragments arranged by size:
2042 1707 678 648 516 359 159
MspA1I CmG'CkG
Cuts at: 1606 2202 2292 2581 3525 4202 5350 5595 1606
Size: 596 90 289 944 677 1148 245 2120
Fragments arranged by size:
2120 1148 944 677 596 289 245 90
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4618
Fragments arranged by size:
4618 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6109
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6109
NotI GC'GGCC_GC
Cuts at: 3556 3556
Size: 6109
NsiI A_TGCA'T
Cuts at: 2320 2320
Size: 6109
NspI r_CATG'y
Cuts at: 1132 1229 3354 3467 5012 1132
Size: 97 2125 113 1545 2229
Fragments arranged by size:
2229 2125 1545 113 97
PasI CC'CwG_GG
Cuts at: 1348 2276 3130 1348
Size: 928 854 4327
Fragments arranged by size:
4327 928 854
PciI A'CATG_T
Cuts at: 5008 5008
Size: 6109
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3078 1108
Size: 1970 4139
PmeI GTTT'AAAC
Cuts at: 3413 3413
Size: 6109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4178 4210 5691 5723 4178
Size: 32 1481 32 4564
Fragments arranged by size:
4564 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2598 2636 2598
Size: 38 6071
PshAI GACnn'nnGTC
Cuts at: 1613 2939 3011 1613
Size: 1326 72 4711
Fragments arranged by size:
4711 1326 72
PsiI TTA'TAA
Cuts at: 3679 3679
Size: 6109
PspOMI G'GGCC_C
Cuts at: 2279 2279
Size: 6109
PstI C_TGCA'G
Cuts at: 830 2580 3461 830
Size: 1750 881 3478
Fragments arranged by size:
3478 1750 881
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6109
PvuII CAG'CTG
Cuts at: 2202 2581 2202
Size: 379 5730
SacI G_AGCT'C
Cuts at: 2856 2856
Size: 6109
SalI G'TCGA_C
Cuts at: 1936 3451 1936
Size: 1515 4594
SbfI CC_TGCA'GG
Cuts at: 3461 3461
Size: 6109
ScaI AGT'ACT
Cuts at: 2810 4357 6069 2810
Size: 1547 1712 2850
Fragments arranged by size:
2850 1712 1547
SfcI C'TryA_G
Cuts at: 826 1045 2197 2576 3457 4592 5273 5464 826
Size: 219 1152 379 881 1135 681 191 1471
Fragments arranged by size:
1471 1152 1135 881 681 379 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6109
SmaI CCC'GGG
Cuts at: 1872 3436 1872
Size: 1564 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4213 5114 5376 820
Size: 197 926 2270 901 262 1553
Fragments arranged by size:
2270 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3900 493
Size: 3407 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6109
SphI G_CATG'C
Cuts at: 1132 3467 1132
Size: 2335 3774
SspI AAT'ATT
Cuts at: 5 52 4033 5
Size: 47 3981 2081
Fragments arranged by size:
3981 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6109
StyI C'CwwG_G
Cuts at: 513 1065 2004 3548 513
Size: 552 939 1544 3074
Fragments arranged by size:
3074 1544 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2357 4136 4153 4306 4491 4936 325
Size: 1255 777 1779 17 153 185 445 1498
Fragments arranged by size:
1779 1498 1255 777 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2808 4355 6067
Size: 274 80 33 51 1443 831 1547 1712
Cuts at: 6067 96
Size: 138
Fragments arranged by size:
1712 1547 1443 831 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6069 6101 6069
Size: 32 6077
Tth111I GACn'n_nGTC
Cuts at: 1316 2687 1316
Size: 1371 4738
XbaI T'CTAG_A
Cuts at: 3445 3445
Size: 6109
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2189 2189
Size: 6109
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6109
XmaI C'CCGG_G
Cuts at: 1870 3434 1870
Size: 1564 4545
XmnI GAAnn'nnTTC
Cuts at: 4238 4238
Size: 6109
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3917 276
Size: 53 83 186 936 2383 2468
Fragments arranged by size:
2468 2383 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI Bsu36I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI