Restriction map for
pFN21AE4447
(Circular) (Six-base) MAPSORT of: pFN21AE4447.seq Check: 1570 from: 1 to: 6091
pFN21AE4447 6091 bp
With 182 enzymes: SgfI *
March 28, 2012 13:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3197 3901 278
Size: 53 83 186 936 1661 704 2468
Fragments arranged by size:
2468 1661 936 704 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2610 3434 1937
Size: 673 824 4594
Fragments arranged by size:
4594 824 673
Acc65I G'GTAC_C
Cuts at: 3412 3412
Size: 6091
AclI AA'CG_TT
Cuts at: 1563 4218 4591 1563
Size: 2655 373 3063
Fragments arranged by size:
3063 2655 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2622 3249 4154 5538 5806 1632
Size: 990 627 905 1384 268 1917
Fragments arranged by size:
1917 1384 990 905 627 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5894
AflIII A'CryG_T
Cuts at: 1203 2295 2400 4990 5967 1203
Size: 1092 105 2590 977 1327
Fragments arranged by size:
2590 1327 1092 977 105
AgeI A'CCGG_T
Cuts at: 4922 4922
Size: 6091
AhdI GACnn_n'nnGTC
Cuts at: 3286 4820 3286
Size: 1534 4557
AleI CACnn'nnGTG
Cuts at: 1166 2225 2891 1166
Size: 1059 666 4366
Fragments arranged by size:
4366 1059 666
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6091
AlwNI CAG_nnn'CTG
Cuts at: 3834 5406 3834
Size: 1572 4519
ApaI G_GGCC'C
Cuts at: 2346 2346
Size: 6091
ApaLI G'TGCA_C
Cuts at: 4148 5304 4148
Size: 1156 4935
ApoI r'AATT_y
Cuts at: 1493 2573 3400 3626 3778 5887 1493
Size: 1080 827 226 152 2109 1697
Fragments arranged by size:
2109 1697 1080 827 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4645 160
Size: 872 3613 1606
Fragments arranged by size:
3613 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
AvaI C'yCGr_G
Cuts at: 1870 1943 2201 3416 1870
Size: 73 258 1215 4545
Fragments arranged by size:
4545 1215 258 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3250 3283 3250
Size: 33 6058
BamHI G'GATC_C
Cuts at: 3421 3788 3421
Size: 367 5724
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2480 3412 4867 618
Size: 325 217 627 693 932 1455 1842
Fragments arranged by size:
1842 1455 932 693 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2172 2346 3410 1391
Size: 781 174 1064 4072
Fragments arranged by size:
4072 1064 781 174
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2989 3242 928
Size: 971 1090 253 3777
Fragments arranged by size:
3777 1090 971 253
BbvCI CC'TCA_GC
Cuts at: 2912 2912
Size: 6091
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3177 3211 4282 4316 1903
Size: 34 1240 34 1071 34 3678
Fragments arranged by size:
3678 1240 1071 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3983 5193 3983
Size: 1210 4881
BclI T'GATC_A
Cuts at: 1548 2037 1548
Size: 489 5602
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4702 136
Size: 107 122 71 631 605 3030 1525
Fragments arranged by size:
3030 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2385 6086 2385
Size: 3701 2390
BlpI GC'TnA_GC
Cuts at: 3508 3508
Size: 6091
Bme1580I G_kGCm'C
Cuts at: 1790 2346 2525 2705 4152 5308 1790
Size: 556 179 180 1447 1156 2573
Fragments arranged by size:
2573 1447 1156 556 180 179
BmgBI CAC'GTC
Cuts at: 1318 2296 1318
Size: 978 5113
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3150 3299 3346
Size: 416 53 83 399 24 1733 149 47
Cuts at: 3346 3822 4780 442
Size: 476 958 1753
Fragments arranged by size:
1753 1733 958 476 416 399 149 83
53 47 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2489 2647 4751 1239
Size: 1250 158 2104 2579
Fragments arranged by size:
2579 2104 1250 158
Bpu10I CC'TnA_GC
Cuts at: 2912 2912
Size: 6091
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2555 4216 5081 5379 2555
Size: 1661 865 298 3267
Fragments arranged by size:
3267 1661 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2120 4754 882
Size: 617 621 2634 2219
Fragments arranged by size:
2634 2219 621 617
BsaAI yAC'GTr
Cuts at: 493 1204 3882 493
Size: 711 2678 2702
Fragments arranged by size:
2702 2678 711
BsaBI GATnn'nnATC
Cuts at: 6085 6085
Size: 6091
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3194 3898 4280 4915
Size: 53 83 186 936 1661 704 382 635
Cuts at: 4915 275
Size: 1451
Fragments arranged by size:
1661 1451 936 704 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4524 4922 5196 5343 5754 4524
Size: 398 274 147 411 4861
Fragments arranged by size:
4861 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5866
Fragments arranged by size:
5866 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6091
BseYI C'CCAG_C
Cuts at: 2206 2863 3048 3184 5294 5790 2206
Size: 657 185 136 2110 496 2507
Fragments arranged by size:
2507 2110 657 496 185 136
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5518
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3541 4302 5330 1540
Size: 275 186 1540 761 1028 2301
Fragments arranged by size:
2301 1540 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4152 4237 5308 4152
Size: 85 1071 4935
Fragments arranged by size:
4935 1071 85
BsmI GAATG_Cn'
Cuts at: 3602 3695 3602
Size: 93 5998
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2172 2346 2525 2705 3410 4152 4237
Size: 399 382 174 179 180 705 742 85
Cuts at: 4237 5308 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 705 399 382 180 179
174 85
BspEI T'CCGG_A
Cuts at: 5754 5754
Size: 6091
BspHI T'CATG_A
Cuts at: 2034 2742 3980 5710 2034
Size: 708 1238 1730 2415
Fragments arranged by size:
2415 1730 1238 708
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3446 844
Size: 2602 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3978 1394
Size: 429 2155 3507
Fragments arranged by size:
3507 2155 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4586 4760 65
Size: 1183 3338 174 1396
Fragments arranged by size:
3338 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3361 4735 4922 1700
Size: 110 1551 1374 187 2869
Fragments arranged by size:
2869 1551 1374 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6091
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6091
BssSI C'ACGA_G
Cuts at: 4151 5163 4151
Size: 1012 5079
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6091
BstBI TT'CG_AA
Cuts at: 4895 4895
Size: 6091
BstEII G'GTnAC_C
Cuts at: 1184 2884 1184
Size: 1700 4391
BstXI CCAn_nnnn'nTGG
Cuts at: 2161 2641 2962 2161
Size: 480 321 5290
Fragments arranged by size:
5290 480 321
BstYI r'GATC_y
Cuts at: 1972 2157 2385 3421 3788 4173 4190 5642 5728
Size: 185 228 1036 367 385 17 1452 86
Cuts at: 5728 5740 6086 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1036 385 367 346 228 185
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2889 2961 3021 513
Size: 552 939 885 72 60 3583
Fragments arranged by size:
3583 939 885 552 72 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2413 487
Size: 683 56 1187 4165
Fragments arranged by size:
4165 1187 683 56
BtsI GCAGTG_nn'
Cuts at: 3607 4401 4421 3607
Size: 794 20 5277
Fragments arranged by size:
5277 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6091
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6056
DraI TTT'AAA
Cuts at: 3395 3395
Size: 6091
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2243 5098 809
Size: 507 927 2855 1802
Fragments arranged by size:
2855 1802 927 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3538 4427 6056
Size: 54 1425 50 275 18 1708 889 1629
Cuts at: 6056 8
Size: 43
Fragments arranged by size:
1708 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3538 1537
Size: 275 1726 4090
Fragments arranged by size:
4090 1726 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3225 4021 1353
Size: 1872 796 3423
Fragments arranged by size:
3423 1872 796
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2666 4678 5052 5198 387
Size: 1064 138 1077 2012 374 146 1280
Fragments arranged by size:
2012 1280 1077 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2489 2622 2647 3249 4154 4751 5538
Size: 393 857 133 25 602 905 597 787
Cuts at: 5538 5806 1239
Size: 268 1524
Fragments arranged by size:
1524 905 857 787 602 597 393 268
133 25
EcoO109I rG'GnC_Cy
Cuts at: 2342 2342
Size: 6091
EcoRI G'AATT_C
Cuts at: 3400 3778 3400
Size: 378 5713
FspI TGC'GCA
Cuts at: 2556 4597 2556
Size: 2041 4050
FspAI rTGC'GCAy
Cuts at: 2556 2556
Size: 6091
HaeII r_GCGC'y
Cuts at: 1678 2356 2925 3008 5238 1678
Size: 678 569 83 2230 2531
Fragments arranged by size:
2531 2230 678 569 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2202 2234 3272 3304 4732
Size: 32 135 32 630 32 1038 32 1428
Cuts at: 4732 4764 4806 4838 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1428 1038 630 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3123 3435 3681 669
Size: 988 281 1185 312 246 3079
Fragments arranged by size:
3079 1185 988 312 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6091
HpaI GTT'AAC
Cuts at: 3681 3681
Size: 6091
KpnI G_GTAC'C
Cuts at: 3416 3416
Size: 6091
MfeI C'AATT_G
Cuts at: 3690 3690
Size: 6091
MluI A'CGCG_T
Cuts at: 5967 5967
Size: 6091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2292 2329 5205 5389 1305
Size: 987 37 2876 184 2007
Fragments arranged by size:
2876 2007 987 184 37
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4269
Fragments arranged by size:
4269 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2225 2276 2294 2741 2891 2960 2978
Size: 648 1059 51 18 447 150 69 18
Cuts at: 2978 4049 4408 4567 518
Size: 1071 359 159 2042
Fragments arranged by size:
2042 1071 1059 648 447 359 159 150
69 51 18 18
MspA1I CmG'CkG
Cuts at: 1606 3507 4184 5332 5577 1606
Size: 1901 677 1148 245 2120
Fragments arranged by size:
2120 1901 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2961 513
Size: 552 939 957 3643
Fragments arranged by size:
3643 957 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6091
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6091
NotI GC'GGCC_GC
Cuts at: 3538 3538
Size: 6091
NspI r_CATG'y
Cuts at: 1132 1229 3449 4994 1132
Size: 97 2220 1545 2229
Fragments arranged by size:
2229 2220 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6091
PciI A'CATG_T
Cuts at: 4990 4990
Size: 6091
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2686 2967 1108
Size: 1578 281 4232
Fragments arranged by size:
4232 1578 281
PmeI GTTT'AAAC
Cuts at: 3395 3395
Size: 6091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 5673 5705 4160
Size: 32 1481 32 4546
Fragments arranged by size:
4546 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6091
PsiI TTA'TAA
Cuts at: 3661 3661
Size: 6091
PspOMI G'GGCC_C
Cuts at: 2342 2342
Size: 6091
PstI C_TGCA'G
Cuts at: 830 2518 3443 830
Size: 1688 925 3478
Fragments arranged by size:
3478 1688 925
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6091
SalI G'TCGA_C
Cuts at: 1936 3433 1936
Size: 1497 4594
SbfI CC_TGCA'GG
Cuts at: 3443 3443
Size: 6091
ScaI AGT'ACT
Cuts at: 4339 6051 4339
Size: 1712 4379
SfcI C'TryA_G
Cuts at: 826 1045 2514 3439 4574 5255 5446 826
Size: 219 1469 925 1135 681 191 1471
Fragments arranged by size:
1471 1469 1135 925 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6091
SmaI CCC'GGG
Cuts at: 1872 2203 3418 1872
Size: 331 1215 4545
Fragments arranged by size:
4545 1215 331
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2534 4195 5096 5358 820
Size: 197 926 591 1661 901 262 1553
Fragments arranged by size:
1661 1553 926 901 591 262 197
SnaBI TAC'GTA
Cuts at: 493 3882 493
Size: 3389 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6091
SphI G_CATG'C
Cuts at: 1132 3449 1132
Size: 2317 3774
SrfI GCCC'GGGC
Cuts at: 2203 2203
Size: 6091
SspI AAT'ATT
Cuts at: 5 52 4015 5
Size: 47 3963 2081
Fragments arranged by size:
3963 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6091
StyI C'CwwG_G
Cuts at: 513 1065 2004 2961 3072 3530 513
Size: 552 939 957 111 458 3074
Fragments arranged by size:
3074 957 939 552 458 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2249 4118 4135 4288 4473 4918 325
Size: 1255 669 1869 17 153 185 445 1498
Fragments arranged by size:
1869 1498 1255 669 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2283 4337 6049
Size: 274 80 33 51 1443 306 2054 1712
Cuts at: 6049 96
Size: 138
Fragments arranged by size:
2054 1712 1443 306 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6051 6083 6051
Size: 32 6059
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6091
XbaI T'CTAG_A
Cuts at: 3427 3427
Size: 6091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2638 2641 2638
Size: 3 6088
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6091
XmaI C'CCGG_G
Cuts at: 1870 2201 3416 1870
Size: 331 1215 4545
Fragments arranged by size:
4545 1215 331
XmnI GAAnn'nnTTC
Cuts at: 2328 4220 2328
Size: 1892 4199
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3195 3899 276
Size: 53 83 186 936 1661 704 2468
Fragments arranged by size:
2468 1661 936 704 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SwaI