Restriction map for
pFN21AE4444
(Circular) (Six-base) MAPSORT of: pFN21AE4444.seq Check: 5570 from: 1 to: 6085
pFN21AE4444 6085 bp
With 182 enzymes: SgfI *
March 28, 2012 14:02 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3895 278
Size: 53 83 186 936 2359 2468
Fragments arranged by size:
2468 2359 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3267 3428 1937
Size: 1330 161 4594
Fragments arranged by size:
4594 1330 161
Acc65I G'GTAC_C
Cuts at: 3406 3406
Size: 6085
AclI AA'CG_TT
Cuts at: 1563 4212 4585 1563
Size: 2649 373 3063
Fragments arranged by size:
3063 2649 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2739 4148 5532 5800 1632
Size: 1107 1409 1384 268 1917
Fragments arranged by size:
1917 1409 1384 1107 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5888
AflIII A'CryG_T
Cuts at: 1203 4984 5961 1203
Size: 3781 977 1327
Fragments arranged by size:
3781 1327 977
AgeI A'CCGG_T
Cuts at: 4916 4916
Size: 6085
AhdI GACnn_n'nnGTC
Cuts at: 4814 4814
Size: 6085
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6085
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6085
AlwNI CAG_nnn'CTG
Cuts at: 3828 5400 3828
Size: 1572 4513
ApaI G_GGCC'C
Cuts at: 2490 2614 2490
Size: 124 5961
ApaLI G'TGCA_C
Cuts at: 3063 4142 5298 3063
Size: 1079 1156 3850
Fragments arranged by size:
3850 1156 1079
ApoI r'AATT_y
Cuts at: 1493 2645 3394 3620 3772 5881 1493
Size: 1152 749 226 152 2109 1697
Fragments arranged by size:
2109 1697 1152 749 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4639 160
Size: 872 3607 1606
Fragments arranged by size:
3607 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6085
AvaI C'yCGr_G
Cuts at: 1870 1943 3410 1870
Size: 73 1467 4545
Fragments arranged by size:
4545 1467 73
BamHI G'GATC_C
Cuts at: 3052 3415 3782 3052
Size: 363 367 5355
Fragments arranged by size:
5355 367 363
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2576 3406 4861 618
Size: 325 217 627 789 830 1455 1842
Fragments arranged by size:
1842 1455 830 789 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2490 2614 3255 3404 1391
Size: 1099 124 641 149 4072
Fragments arranged by size:
4072 1099 641 149 124
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2635 928
Size: 971 736 4378
Fragments arranged by size:
4378 971 736
BbvCI CC'TCA_GC
Cuts at: 2231 2231
Size: 6085
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4276 4310 1903
Size: 34 2339 34 3678
Fragments arranged by size:
3678 2339 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3190 3977 5187 3190
Size: 787 1210 4088
Fragments arranged by size:
4088 1210 787
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6085
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4696 136
Size: 107 122 71 631 605 3024 1525
Fragments arranged by size:
3024 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6080 6080
Size: 6085
BlpI GC'TnA_GC
Cuts at: 2186 3502 2186
Size: 1316 4769
Bme1580I G_kGCm'C
Cuts at: 1790 2490 2581 2614 3067 3330 4146 5302 1790
Size: 700 91 33 453 263 816 1156 2573
Fragments arranged by size:
2573 1156 816 700 453 263 91 33
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6085
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2171 2617 3816
Size: 416 53 83 399 24 754 446 1199
Cuts at: 3816 4774 442
Size: 958 1753
Fragments arranged by size:
1753 1199 958 754 446 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6085
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2639 4745 1239
Size: 1400 2106 2579
Fragments arranged by size:
2579 2106 1400
Bpu10I CC'TnA_GC
Cuts at: 2231 2231
Size: 6085
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4210 5075 5373 4210
Size: 865 298 4922
Fragments arranged by size:
4922 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2305 4748 882
Size: 617 806 2443 2219
Fragments arranged by size:
2443 2219 806 617
BsaAI yAC'GTr
Cuts at: 493 1204 2839 3876 493
Size: 711 1635 1037 2702
Fragments arranged by size:
2702 1635 1037 711
BsaBI GATnn'nnATC
Cuts at: 6079 6079
Size: 6085
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3892 4274 4909 275
Size: 53 83 186 936 2359 382 635 1451
Fragments arranged by size:
2359 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4518 4916 5190 5337 5748 4518
Size: 398 274 147 411 4855
Fragments arranged by size:
4855 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2150 2180 3093 3123 1178
Size: 30 165 30 747 30 913 30 4140
Fragments arranged by size:
4140 913 747 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2937 3114 1186
Size: 1751 177 4157
Fragments arranged by size:
4157 1751 177
BseYI C'CCAG_C
Cuts at: 2201 2623 5288 5784 2201
Size: 422 2665 496 2502
Fragments arranged by size:
2665 2502 496 422
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2728 3307 1163
Size: 573 992 579 3941
Fragments arranged by size:
3941 992 579 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3535 4296 5324 1540
Size: 275 186 1534 761 1028 2301
Fragments arranged by size:
2301 1534 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2682 2932 3067 4146 4231 5302 2682
Size: 250 135 1079 85 1071 3465
Fragments arranged by size:
3465 1079 1071 250 135 85
BsmI GAATG_Cn'
Cuts at: 2748 3596 3689 2748
Size: 848 93 5144
Fragments arranged by size:
5144 848 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2490 2581 2614 2682 2932 3067 3255
Size: 399 700 91 33 68 250 135 188
Cuts at: 3255 3330 3404 4146 4231 5302 1391
Size: 75 74 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 700 399 250 188 135
91 85 75 74 68 33
BspEI T'CCGG_A
Cuts at: 5748 5748
Size: 6085
BspHI T'CATG_A
Cuts at: 2337 3974 5704 2337
Size: 1637 1730 2718
Fragments arranged by size:
2718 1730 1637
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3440 844
Size: 2596 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3972 1394
Size: 429 2149 3507
Fragments arranged by size:
3507 2149 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2862 2910 4580 4754 65
Size: 1183 1614 48 1670 174 1396
Fragments arranged by size:
1670 1614 1396 1183 174 48
BsrFI r'CCGG_y
Cuts at: 1700 1810 2289 4729 4916 1700
Size: 110 479 2440 187 2869
Fragments arranged by size:
2869 2440 479 187 110
BsrGI T'GTAC_A
Cuts at: 96 2211 3318 96
Size: 2115 1107 2863
Fragments arranged by size:
2863 2115 1107
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6085
BssSI C'ACGA_G
Cuts at: 2345 4145 5157 2345
Size: 1800 1012 3273
Fragments arranged by size:
3273 1800 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6085
BstBI TT'CG_AA
Cuts at: 4889 4889
Size: 6085
BstEII G'GTnAC_C
Cuts at: 1184 2585 1184
Size: 1401 4684
BstXI CCAn_nnnn'nTGG
Cuts at: 2981 2981
Size: 6085
BstYI r'GATC_y
Cuts at: 1972 3052 3415 3782 4167 4184 5636 5722 5734
Size: 1080 363 367 385 17 1452 86 12
Cuts at: 5734 6080 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1080 385 367 363 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2769 2769
Size: 6085
BtgI C'CryG_G
Cuts at: 513 1065 2004 2262 3046 513
Size: 552 939 258 784 3552
Fragments arranged by size:
3552 939 784 552 258
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5346
Fragments arranged by size:
5346 683 56
BtsI GCAGTG_nn'
Cuts at: 2284 2918 3601 4395 4415 2284
Size: 634 683 794 20 3954
Fragments arranged by size:
3954 794 683 634 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6085
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6050
DraI TTT'AAA
Cuts at: 3389 3389
Size: 6085
DraIII CAC_nnn'GTG
Cuts at: 2688 2688
Size: 6085
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2914 5092 809
Size: 507 1598 2178 1802
Fragments arranged by size:
2178 1802 1598 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2029 2124 2274
Size: 54 1425 50 275 18 199 95 150
Cuts at: 2274 3532 4421 6050 8
Size: 1258 889 1629 43
Fragments arranged by size:
1629 1425 1258 889 275 199 150 95
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3532 1537
Size: 275 1720 4090
Fragments arranged by size:
4090 1720 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2131 2564 3260 4015 1353
Size: 778 433 696 755 3423
Fragments arranged by size:
3423 778 755 696 433
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4672 5046 5192 387
Size: 1064 138 3083 374 146 1280
Fragments arranged by size:
3083 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2639 2739 4148 4745 5532 5800 1239
Size: 393 1007 100 1409 597 787 268 1524
Fragments arranged by size:
1524 1409 1007 787 597 393 268 100
EcoO109I rG'GnC_Cy
Cuts at: 2539 2773 2539
Size: 234 5851
EcoRI G'AATT_C
Cuts at: 2645 3394 3772 2645
Size: 749 378 4958
Fragments arranged by size:
4958 749 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2744 2744
Size: 6085
FspI TGC'GCA
Cuts at: 4591 4591
Size: 6085
HaeII r_GCGC'y
Cuts at: 1678 5232 1678
Size: 3554 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2304 2336 2501 2533 4726
Size: 32 135 32 732 32 165 32 2193
Cuts at: 4726 4758 4800 4832 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2193 732 165 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3429 3675 669
Size: 988 281 1491 246 3079
Fragments arranged by size:
3079 1491 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6085
HpaI GTT'AAC
Cuts at: 3675 3675
Size: 6085
KpnI G_GTAC'C
Cuts at: 3410 3410
Size: 6085
MfeI C'AATT_G
Cuts at: 3684 3684
Size: 6085
MluI A'CGCG_T
Cuts at: 5961 5961
Size: 6085
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2248 3027 3091 5199 5383 1305
Size: 943 779 64 2108 184 2007
Fragments arranged by size:
2108 2007 943 779 184 64
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2126 10
Size: 54 1425 343 294 3969
Fragments arranged by size:
3969 1425 343 294 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2126 2550 4043 4402 4561 518
Size: 648 960 424 1493 359 159 2042
Fragments arranged by size:
2042 1493 960 648 424 359 159
MspA1I CmG'CkG
Cuts at: 1606 3048 3501 4178 5326 5571 1606
Size: 1442 453 677 1148 245 2120
Fragments arranged by size:
2120 1442 1148 677 453 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4594
Fragments arranged by size:
4594 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6085
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6085
NotI GC'GGCC_GC
Cuts at: 3532 3532
Size: 6085
NspI r_CATG'y
Cuts at: 1132 1229 2944 3010 3443 4988 1132
Size: 97 1715 66 433 1545 2229
Fragments arranged by size:
2229 1715 1545 433 97 66
PasI CC'CwG_GG
Cuts at: 1348 2329 2581 1348
Size: 981 252 4852
Fragments arranged by size:
4852 981 252
PciI A'CATG_T
Cuts at: 4984 4984
Size: 6085
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6085
PmeI GTTT'AAAC
Cuts at: 3389 3389
Size: 6085
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4154 4186 5667 5699 4154
Size: 32 1481 32 4540
Fragments arranged by size:
4540 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2773 2773
Size: 6085
PshAI GACnn'nnGTC
Cuts at: 1613 2177 1613
Size: 564 5521
PsiI TTA'TAA
Cuts at: 3655 3655
Size: 6085
PspOMI G'GGCC_C
Cuts at: 2486 2610 2486
Size: 124 5961
PstI C_TGCA'G
Cuts at: 830 2259 3437 830
Size: 1429 1178 3478
Fragments arranged by size:
3478 1429 1178
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6085
SacII CC_GC'GG
Cuts at: 3049 3049
Size: 6085
SalI G'TCGA_C
Cuts at: 1936 3427 1936
Size: 1491 4594
SapI GCTCTTCn'nnn_
Cuts at: 3260 3260
Size: 6085
SbfI CC_TGCA'GG
Cuts at: 3437 3437
Size: 6085
ScaI AGT'ACT
Cuts at: 4333 6045 4333
Size: 1712 4373
SfcI C'TryA_G
Cuts at: 826 1045 2255 2601 3058 3433 4568 5249 5440
Size: 219 1210 346 457 375 1135 681 191
Cuts at: 5440 826
Size: 1471
Fragments arranged by size:
1471 1210 1135 681 457 375 346 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6085
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6085
SmaI CCC'GGG
Cuts at: 1872 3412 1872
Size: 1540 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4189 5090 5352 820
Size: 197 926 2246 901 262 1553
Fragments arranged by size:
2246 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2839 3876 493
Size: 2346 1037 2702
Fragments arranged by size:
2702 2346 1037
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6085
SphI G_CATG'C
Cuts at: 1132 3443 1132
Size: 2311 3774
SspI AAT'ATT
Cuts at: 5 52 4009 5
Size: 47 3957 2081
Fragments arranged by size:
3957 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6085
StyI C'CwwG_G
Cuts at: 513 1065 2004 2405 3524 513
Size: 552 939 401 1119 3074
Fragments arranged by size:
3074 1119 939 552 401
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2585 4112 4129 4282 4467 4912 325
Size: 1255 1005 1527 17 153 185 445 1498
Fragments arranged by size:
1527 1498 1255 1005 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2211 3318 4331
Size: 274 80 33 51 1443 234 1107 1013
Cuts at: 4331 6043 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1107 1013 274 234 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2276 2308 6045 6077 2276
Size: 32 3737 32 2284
Fragments arranged by size:
3737 2284 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6085
XbaI T'CTAG_A
Cuts at: 3421 3421
Size: 6085
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6085
XmaI C'CCGG_G
Cuts at: 1870 3410 1870
Size: 1540 4545
XmnI GAAnn'nnTTC
Cuts at: 4214 4214
Size: 6085
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3893 276
Size: 53 83 186 936 2359 2468
Fragments arranged by size:
2468 2359 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI PvuII RsrII SacI SanDI
SexAI SfiI SfoI SrfI SwaI XcmI