Restriction map for
pFN21AE4443
(Circular) (Six-base) MAPSORT of: pFN21AE4443.seq Check: 2075 from: 1 to: 6082
pFN21AE4443 6082 bp
With 182 enzymes: SgfI *
March 28, 2012 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3892 278
Size: 53 83 186 936 2356 2468
Fragments arranged by size:
2468 2356 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3034 3425 1937
Size: 1097 391 4594
Fragments arranged by size:
4594 1097 391
Acc65I G'GTAC_C
Cuts at: 3245 3403 3245
Size: 158 5924
AclI AA'CG_TT
Cuts at: 1563 4209 4582 1563
Size: 2646 373 3063
Fragments arranged by size:
3063 2646 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2502 3152 4145 5529 5797 1632
Size: 870 650 993 1384 268 1917
Fragments arranged by size:
1917 1384 993 870 650 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5885
AflIII A'CryG_T
Cuts at: 1203 4981 5958 1203
Size: 3778 977 1327
Fragments arranged by size:
3778 1327 977
AgeI A'CCGG_T
Cuts at: 4913 4913
Size: 6082
AhdI GACnn_n'nnGTC
Cuts at: 4811 4811
Size: 6082
AleI CACnn'nnGTG
Cuts at: 1166 2564 1166
Size: 1398 4684
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6082
AlwNI CAG_nnn'CTG
Cuts at: 3130 3293 3825 5397 3130
Size: 163 532 1572 3815
Fragments arranged by size:
3815 1572 532 163
ApaLI G'TGCA_C
Cuts at: 2705 4139 5295 2705
Size: 1434 1156 3492
Fragments arranged by size:
3492 1434 1156
ApoI r'AATT_y
Cuts at: 1493 2765 3391 3617 3769 5878 1493
Size: 1272 626 226 152 2109 1697
Fragments arranged by size:
2109 1697 1272 626 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4636 160
Size: 872 3604 1606
Fragments arranged by size:
3604 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6082
AvaI C'yCGr_G
Cuts at: 1870 1943 3234 3407 1870
Size: 73 1291 173 4545
Fragments arranged by size:
4545 1291 173 73
BamHI G'GATC_C
Cuts at: 3412 3779 3412
Size: 367 5715
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3245 3403 4858 618
Size: 325 217 627 1458 158 1455 1842
Fragments arranged by size:
1842 1458 1455 627 325 217 158
BanII G_rGCy'C
Cuts at: 1391 2748 3401 1391
Size: 1357 653 4072
Fragments arranged by size:
4072 1357 653
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5111
BbvCI CC'TCA_GC
Cuts at: 3095 3095
Size: 6082
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4273 4307 1903
Size: 34 2336 34 3678
Fragments arranged by size:
3678 2336 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3974 5184 3974
Size: 1210 4872
BclI T'GATC_A
Cuts at: 1548 2007 2635 1548
Size: 459 628 4995
Fragments arranged by size:
4995 628 459
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2274 4693 136
Size: 107 122 71 631 605 602 2419 1525
Fragments arranged by size:
2419 1525 631 605 602 122 107 71
BglII A'GATC_T
Cuts at: 2895 6077 2895
Size: 3182 2900
BlpI GC'TnA_GC
Cuts at: 3499 3499
Size: 6082
Bme1580I G_kGCm'C
Cuts at: 1790 2233 2709 3078 3175 4143 5299 1790
Size: 443 476 369 97 968 1156 2573
Fragments arranged by size:
2573 1156 968 476 443 369 97
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6082
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2194 3813 4771
Size: 416 53 83 399 24 777 1619 958
Cuts at: 4771 442
Size: 1753
Fragments arranged by size:
1753 1619 958 777 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6082
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2042 2195 4742 1239
Size: 803 153 2547 2579
Fragments arranged by size:
2579 2547 803 153
Bpu10I CC'TnA_GC
Cuts at: 2814 3095 2814
Size: 281 5801
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4207 5072 5370 4207
Size: 865 298 4919
Fragments arranged by size:
4919 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4745 882
Size: 617 3246 2219
Fragments arranged by size:
3246 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3113 3873 493
Size: 711 1909 760 2702
Fragments arranged by size:
2702 1909 760 711
BsaBI GATnn'nnATC
Cuts at: 6076 6076
Size: 6082
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3889 4271 4906 275
Size: 53 83 186 936 2356 382 635 1451
Fragments arranged by size:
2356 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2463 4515 4913 5187 5334 5745 2463
Size: 2052 398 274 147 411 2800
Fragments arranged by size:
2800 2052 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5857
Fragments arranged by size:
5857 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2885 3335 1186
Size: 1699 450 3933
Fragments arranged by size:
3933 1699 450
BseYI C'CCAG_C
Cuts at: 2233 5285 5781 2233
Size: 3052 496 2534
Fragments arranged by size:
3052 2534 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5509
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3532 4293 5321 1540
Size: 275 186 1531 761 1028 2301
Fragments arranged by size:
2301 1531 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2709 4143 4228 5299 2709
Size: 1434 85 1071 3492
Fragments arranged by size:
3492 1434 1071 85
BsmI GAATG_Cn'
Cuts at: 3593 3686 3593
Size: 93 5989
BsmBI CGTCTCn'nnnn_
Cuts at: 2655 2655
Size: 6082
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2233 2709 2748 3078 3175 3401 4143
Size: 399 443 476 39 330 97 226 742
Cuts at: 4143 4228 5299 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 476 443 399 330 226
97 85 39
BspEI T'CCGG_A
Cuts at: 5745 5745
Size: 6082
BspHI T'CATG_A
Cuts at: 2769 3971 5701 2769
Size: 1202 1730 3150
Fragments arranged by size:
3150 1730 1202
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3437 844
Size: 2593 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3969 1394
Size: 429 2146 3507
Fragments arranged by size:
3507 2146 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4577 4751 65
Size: 1183 3329 174 1396
Fragments arranged by size:
3329 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4726 4913 1700
Size: 110 2916 187 2869
Fragments arranged by size:
2916 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6082
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6082
BssSI C'ACGA_G
Cuts at: 4142 5154 4142
Size: 1012 5070
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3343 794
Size: 2549 3533
BstBI TT'CG_AA
Cuts at: 4886 4886
Size: 6082
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6082
BstYI r'GATC_y
Cuts at: 1972 2895 3283 3412 3779 4164 4181 5633 5719
Size: 923 388 129 367 385 17 1452 86
Cuts at: 5719 5731 6077 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 923 388 385 367 346 129
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2031 2270 2740 513
Size: 552 966 239 470 3855
Fragments arranged by size:
3855 966 552 470 239
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5343
Fragments arranged by size:
5343 683 56
BtsI GCAGTG_nn'
Cuts at: 2529 2707 3076 3598 4392 4412 2529
Size: 178 369 522 794 20 4199
Fragments arranged by size:
4199 794 522 369 178 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6082
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6047
DraI TTT'AAA
Cuts at: 3386 3386
Size: 6082
DraIII CAC_nnn'GTG
Cuts at: 2607 2607
Size: 6082
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5089 809
Size: 507 3773 1802
Fragments arranged by size:
3773 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2737 2750 3529
Size: 54 1425 50 275 18 907 13 779
Cuts at: 3529 4418 6047 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 907 889 779 275 54 50
43 18 13
EagI C'GGCC_G
Cuts at: 1537 1812 3529 1537
Size: 275 1717 4090
Fragments arranged by size:
4090 1717 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2444 2478 2529 4012 1353
Size: 1091 34 51 1483 3423
Fragments arranged by size:
3423 1483 1091 51 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3346 4669 5043 5189 387
Size: 1064 138 1757 1323 374 146 1280
Fragments arranged by size:
1757 1323 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2195 2502 3152 4145 4742 5529
Size: 393 410 153 307 650 993 597 787
Cuts at: 5529 5797 1239
Size: 268 1524
Fragments arranged by size:
1524 993 787 650 597 410 393 307
268 153
EcoNI CCTnn'n_nnAGG
Cuts at: 2813 2813
Size: 6082
EcoO109I rG'GnC_Cy
Cuts at: 2121 2142 2149 2259 2776 3127 2121
Size: 21 7 110 517 351 5076
Fragments arranged by size:
5076 517 351 110 21 7
EcoRI G'AATT_C
Cuts at: 2765 3391 3769 2765
Size: 626 378 5078
Fragments arranged by size:
5078 626 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2389 2389
Size: 6082
FspI TGC'GCA
Cuts at: 4588 4588
Size: 6082
HaeII r_GCGC'y
Cuts at: 1678 5229 1678
Size: 3551 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2903 2935 4723 4755 4797
Size: 32 135 32 1331 32 1788 32 42
Cuts at: 4797 4829 1373
Size: 32 2626
Fragments arranged by size:
2626 1788 1331 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2596 2794 3035 3426 3672 669
Size: 988 281 658 198 241 391 246 3079
Fragments arranged by size:
3079 988 658 391 281 246 241 198
HindIII A'AGCT_T
Cuts at: 748 2279 3299 748
Size: 1531 1020 3531
Fragments arranged by size:
3531 1531 1020
HpaI GTT'AAC
Cuts at: 3672 3672
Size: 6082
KpnI G_GTAC'C
Cuts at: 3249 3407 3249
Size: 158 5924
MfeI C'AATT_G
Cuts at: 3681 3681
Size: 6082
MluI A'CGCG_T
Cuts at: 5958 5958
Size: 6082
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5196 5380 1305
Size: 3891 184 2007
Fragments arranged by size:
3891 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4260
Fragments arranged by size:
4260 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2564 4040 4399 4558 518
Size: 648 1398 1476 359 159 2042
Fragments arranged by size:
2042 1476 1398 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2788 3498 4175 5323 5568 1606
Size: 1182 710 677 1148 245 2120
Fragments arranged by size:
2120 1182 1148 710 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2270 2740 513
Size: 552 1205 470 3855
Fragments arranged by size:
3855 1205 552 470
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6082
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6082
NotI GC'GGCC_GC
Cuts at: 3529 3529
Size: 6082
NspI r_CATG'y
Cuts at: 1132 1229 2522 3124 3440 4985 1132
Size: 97 1293 602 316 1545 2229
Fragments arranged by size:
2229 1545 1293 602 316 97
PasI CC'CwG_GG
Cuts at: 1348 3045 3071 1348
Size: 1697 26 4359
Fragments arranged by size:
4359 1697 26
PciI A'CATG_T
Cuts at: 4981 4981
Size: 6082
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2582 1108
Size: 1474 4608
PfoI T'CCnGG_A
Cuts at: 2019 2130 2865 2019
Size: 111 735 5236
Fragments arranged by size:
5236 735 111
PmeI GTTT'AAAC
Cuts at: 3386 3386
Size: 6082
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4151 4183 5664 5696 4151
Size: 32 1481 32 4537
Fragments arranged by size:
4537 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3127 3127
Size: 6082
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6082
PsiI TTA'TAA
Cuts at: 3652 3652
Size: 6082
PstI C_TGCA'G
Cuts at: 830 3434 830
Size: 2604 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6082
SalI G'TCGA_C
Cuts at: 1936 3033 3424 1936
Size: 1097 391 4594
Fragments arranged by size:
4594 1097 391
SbfI CC_TGCA'GG
Cuts at: 3434 3434
Size: 6082
ScaI AGT'ACT
Cuts at: 2222 4330 6042 2222
Size: 2108 1712 2262
Fragments arranged by size:
2262 2108 1712
SexAI A'CCwGG_T
Cuts at: 2575 2575
Size: 6082
SfcI C'TryA_G
Cuts at: 826 1045 3000 3430 4565 5246 5437 826
Size: 219 1955 430 1135 681 191 1471
Fragments arranged by size:
1955 1471 1135 681 430 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6082
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6082
SmaI CCC'GGG
Cuts at: 1872 3409 1872
Size: 1537 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4186 5087 5349 820
Size: 197 926 2243 901 262 1553
Fragments arranged by size:
2243 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3113 3873 493
Size: 2620 760 2702
Fragments arranged by size:
2702 2620 760
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6082
SphI G_CATG'C
Cuts at: 1132 2522 3440 1132
Size: 1390 918 3774
Fragments arranged by size:
3774 1390 918
SspI AAT'ATT
Cuts at: 5 52 4006 5
Size: 47 3954 2081
Fragments arranged by size:
3954 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6082
StyI C'CwwG_G
Cuts at: 513 1065 2138 2270 2740 3521 513
Size: 552 1073 132 470 781 3074
Fragments arranged by size:
3074 1073 781 552 470 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2106 4109 4126 4279 4464 4909 325
Size: 1255 526 2003 17 153 185 445 1498
Fragments arranged by size:
2003 1498 1255 526 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2220 2718 4328
Size: 274 80 33 51 1443 243 498 1610
Cuts at: 4328 6040 96
Size: 1712 138
Fragments arranged by size:
1712 1610 1443 498 274 243 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2342 2374 3165 3197 6042 6074 2342
Size: 32 791 32 2845 32 2350
Fragments arranged by size:
2845 2350 791 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6082
XbaI T'CTAG_A
Cuts at: 3418 3418
Size: 6082
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6082
XmaI C'CCGG_G
Cuts at: 1870 3407 1870
Size: 1537 4545
XmnI GAAnn'nnTTC
Cuts at: 2066 4211 2066
Size: 2145 3937
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3890 276
Size: 53 83 186 936 2356 2468
Fragments arranged by size:
2468 2356 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI