Restriction map for
pFN21AE4441
(Circular) (Six-base) MAPSORT of: pFN21AE4441.seq Check: 6043 from: 1 to: 6079
pFN21AE4441 6079 bp
With 182 enzymes: SgfI *
March 28, 2012 14:02 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2475 3889 278
Size: 53 83 186 936 939 1414 2468
Fragments arranged by size:
2468 1414 939 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2574 3422 1937
Size: 637 848 4594
Fragments arranged by size:
4594 848 637
Acc65I G'GTAC_C
Cuts at: 3400 3400
Size: 6079
AclI AA'CG_TT
Cuts at: 1563 2961 4206 4579 1563
Size: 1398 1245 373 3063
Fragments arranged by size:
3063 1398 1245 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4142 5526 5794 1632
Size: 2510 1384 268 1917
Fragments arranged by size:
2510 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5882
AflIII A'CryG_T
Cuts at: 1203 3217 4978 5955 1203
Size: 2014 1761 977 1327
Fragments arranged by size:
2014 1761 1327 977
AgeI A'CCGG_T
Cuts at: 4910 4910
Size: 6079
AhdI GACnn_n'nnGTC
Cuts at: 4808 4808
Size: 6079
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6079
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6079
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3147 3179 3147
Size: 32 6047
AlwNI CAG_nnn'CTG
Cuts at: 3822 5394 3822
Size: 1572 4507
ApaLI G'TGCA_C
Cuts at: 4136 5292 4136
Size: 1156 4923
ApoI r'AATT_y
Cuts at: 1493 3263 3388 3614 3766 5875 1493
Size: 1770 125 226 152 2109 1697
Fragments arranged by size:
2109 1770 1697 226 152 125
AseI AT'TA_AT
Cuts at: 160 1032 4633 160
Size: 872 3601 1606
Fragments arranged by size:
3601 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
AvaI C'yCGr_G
Cuts at: 1870 1943 3404 1870
Size: 73 1461 4545
Fragments arranged by size:
4545 1461 73
BamHI G'GATC_C
Cuts at: 2301 2519 3409 3776 2301
Size: 218 890 367 4604
Fragments arranged by size:
4604 890 367 218
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2537 3400 4855 618
Size: 325 217 627 750 863 1455 1842
Fragments arranged by size:
1842 1455 863 750 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3398 1391
Size: 2007 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2862 928
Size: 971 963 4145
Fragments arranged by size:
4145 971 963
BbvCI CC'TCA_GC
Cuts at: 2053 2053
Size: 6079
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2484 2518 3103 3137 4270
Size: 34 50 34 463 34 585 34 1133
Cuts at: 4270 4304 1903
Size: 34 3678
Fragments arranged by size:
3678 1133 585 463 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3971 5181 3971
Size: 1210 4869
BclI T'GATC_A
Cuts at: 1548 2559 1548
Size: 1011 5068
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4690 136
Size: 107 122 71 631 605 3018 1525
Fragments arranged by size:
3018 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2972 6074 2972
Size: 3102 2977
BlpI GC'TnA_GC
Cuts at: 3496 3496
Size: 6079
Bme1580I G_kGCm'C
Cuts at: 1790 4140 5296 1790
Size: 2350 1156 2573
Fragments arranged by size:
2573 2350 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6079
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3810 4768 442
Size: 416 53 83 399 24 2393 958 1753
Fragments arranged by size:
2393 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4739 1239
Size: 3500 2579
Bpu10I CC'TnA_GC
Cuts at: 2053 2053
Size: 6079
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3258 4204 5069 5367 3258
Size: 946 865 298 3970
Fragments arranged by size:
3970 946 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4742 882
Size: 617 3243 2219
Fragments arranged by size:
3243 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2676 2985 3870 493
Size: 711 1472 309 885 2702
Fragments arranged by size:
2702 1472 885 711 309
BsaBI GATnn'nnATC
Cuts at: 6073 6073
Size: 6079
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2472 2695 3886 4268
Size: 53 83 186 936 939 223 1191 382
Cuts at: 4268 4903 275
Size: 635 1451
Fragments arranged by size:
1451 1191 939 936 635 382 223 186
83 53
BsaWI w'CCGG_w
Cuts at: 4512 4910 5184 5331 5742 4512
Size: 398 274 147 411 4849
Fragments arranged by size:
4849 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2441 2471 3147 3177 1178
Size: 30 165 30 1038 30 676 30 4080
Fragments arranged by size:
4080 1038 676 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 3104 1186
Size: 904 1014 4161
Fragments arranged by size:
4161 1014 904
BseYI C'CCAG_C
Cuts at: 2193 5282 5778 2193
Size: 3089 496 2494
Fragments arranged by size:
3089 2494 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2446 1163
Size: 573 710 4796
Fragments arranged by size:
4796 710 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2374 2494 3529 4290 5318 1540
Size: 275 186 373 120 1035 761 1028 2301
Fragments arranged by size:
2301 1035 1028 761 373 275 186 120
BsiHKAI G_wGCw'C
Cuts at: 4140 4225 5296 4140
Size: 85 1071 4923
Fragments arranged by size:
4923 1071 85
BsmI GAATG_Cn'
Cuts at: 2623 3590 3683 2623
Size: 967 93 5019
Fragments arranged by size:
5019 967 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2686 2686
Size: 6079
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3398 4140 4225 5296 1391
Size: 399 1608 742 85 1071 2174
Fragments arranged by size:
2174 1608 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5742 5742
Size: 6079
BspHI T'CATG_A
Cuts at: 2913 3968 5698 2913
Size: 1055 1730 3294
Fragments arranged by size:
3294 1730 1055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2910 3434 844
Size: 2066 524 3489
Fragments arranged by size:
3489 2066 524
BsrBI CCG'CTC
Cuts at: 1394 1823 3966 1394
Size: 429 2143 3507
Fragments arranged by size:
3507 2143 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4574 4748 65
Size: 1183 3326 174 1396
Fragments arranged by size:
3326 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4723 4910 1700
Size: 110 2913 187 2869
Fragments arranged by size:
2913 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6079
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6079
BssSI C'ACGA_G
Cuts at: 4139 5151 4139
Size: 1012 5067
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6079
BstBI TT'CG_AA
Cuts at: 2379 4883 2379
Size: 2504 3575
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6079
BstYI r'GATC_y
Cuts at: 1972 2301 2519 2972 3409 3776 4161 4178 5630
Size: 329 218 453 437 367 385 17 1452
Cuts at: 5630 5716 5728 6074 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 453 437 385 367 346 329
218 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2466 2541 2994 513
Size: 552 1401 75 453 3598
Fragments arranged by size:
3598 1401 552 453 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5340
Fragments arranged by size:
5340 683 56
BtsI GCAGTG_nn'
Cuts at: 3595 4389 4409 3595
Size: 794 20 5265
Fragments arranged by size:
5265 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6079
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6044
DraI TTT'AAA
Cuts at: 3383 3383
Size: 6079
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5086 809
Size: 507 3770 1802
Fragments arranged by size:
3770 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2371 3526 4415
Size: 54 1425 50 275 18 541 1155 889
Cuts at: 4415 6044 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1155 889 541 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2371 3526 1537
Size: 275 559 1155 4090
Fragments arranged by size:
4090 1155 559 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4009 1353
Size: 2656 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2126 4666 5040 5186 387
Size: 1064 138 537 2540 374 146 1280
Fragments arranged by size:
2540 1280 1064 537 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4142 4739 5526 5794 1239
Size: 393 2510 597 787 268 1524
Fragments arranged by size:
2510 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3388 3766 3388
Size: 378 5701
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3034 3034
Size: 6079
FspI TGC'GCA
Cuts at: 4585 4585
Size: 6079
HaeII r_GCGC'y
Cuts at: 1678 5226 1678
Size: 3548 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3056 3088 4720 4752 4794
Size: 32 135 32 1484 32 1632 32 42
Cuts at: 4794 4826 1373
Size: 32 2626
Fragments arranged by size:
2626 1632 1484 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2548 2575 3098 3423 3669 669
Size: 988 281 610 27 523 325 246 3079
Fragments arranged by size:
3079 988 610 523 325 281 246 27
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6079
HpaI GTT'AAC
Cuts at: 3669 3669
Size: 6079
KpnI G_GTAC'C
Cuts at: 3404 3404
Size: 6079
MfeI C'AATT_G
Cuts at: 3678 3678
Size: 6079
MluI A'CGCG_T
Cuts at: 5955 5955
Size: 6079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5193 5377 1305
Size: 3888 184 2007
Fragments arranged by size:
3888 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4257
Fragments arranged by size:
4257 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2154 4037 4396 4555 518
Size: 648 988 1883 359 159 2042
Fragments arranged by size:
2042 1883 988 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2224 3495 4172 5320 5565 1606
Size: 618 1271 677 1148 245 2120
Fragments arranged by size:
2120 1271 1148 677 618 245
NcoI C'CATG_G
Cuts at: 513 1065 2466 2541 2994 513
Size: 552 1401 75 453 3598
Fragments arranged by size:
3598 1401 552 453 75
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6079
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6079
NotI GC'GGCC_GC
Cuts at: 3526 3526
Size: 6079
NspI r_CATG'y
Cuts at: 1132 1229 3221 3437 4982 1132
Size: 97 1992 216 1545 2229
Fragments arranged by size:
2229 1992 1545 216 97
PasI CC'CwG_GG
Cuts at: 1348 3003 1348
Size: 1655 4424
PciI A'CATG_T
Cuts at: 3217 4978 3217
Size: 1761 4318
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2241 2995 1108
Size: 1133 754 4192
Fragments arranged by size:
4192 1133 754
PfoI T'CCnGG_A
Cuts at: 2522 2522
Size: 6079
PmeI GTTT'AAAC
Cuts at: 3383 3383
Size: 6079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3147 3179 4148 4180 5661 5693 3147
Size: 32 969 32 1481 32 3533
Fragments arranged by size:
3533 1481 969 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2399 1613
Size: 786 5293
PsiI TTA'TAA
Cuts at: 3649 3649
Size: 6079
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2891 2923 3261 3293 2891
Size: 32 338 32 5677
Fragments arranged by size:
5677 338 32 32
PstI C_TGCA'G
Cuts at: 830 3027 3431 830
Size: 2197 404 3478
Fragments arranged by size:
3478 2197 404
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6079
PvuII CAG'CTG
Cuts at: 2224 2224
Size: 6079
SalI G'TCGA_C
Cuts at: 1936 2573 3421 1936
Size: 637 848 4594
Fragments arranged by size:
4594 848 637
SbfI CC_TGCA'GG
Cuts at: 3431 3431
Size: 6079
ScaI AGT'ACT
Cuts at: 4327 6039 4327
Size: 1712 4367
SfcI C'TryA_G
Cuts at: 826 1045 3023 3427 4562 5243 5434 826
Size: 219 1978 404 1135 681 191 1471
Fragments arranged by size:
1978 1471 1135 681 404 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6079
SmaI CCC'GGG
Cuts at: 1872 3406 1872
Size: 1534 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3273 4183 5084 5346 820
Size: 197 926 1330 910 901 262 1553
Fragments arranged by size:
1553 1330 926 910 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3870 493
Size: 3377 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6079
SphI G_CATG'C
Cuts at: 1132 3437 1132
Size: 2305 3774
SspI AAT'ATT
Cuts at: 5 52 4003 5
Size: 47 3951 2081
Fragments arranged by size:
3951 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6079
StyI C'CwwG_G
Cuts at: 513 1065 2038 2116 2397 2466 2541 2994 3518
Size: 552 973 78 281 69 75 453 524
Cuts at: 3518 513
Size: 3074
Fragments arranged by size:
3074 973 552 524 453 281 78 75
69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2508 2803 3300 4106 4123 4276 4461
Size: 1255 928 295 497 806 17 153 185
Cuts at: 4461 4906 325
Size: 445 1498
Fragments arranged by size:
1498 1255 928 806 497 445 295 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2655 3250 4325
Size: 274 80 33 51 1443 678 595 1075
Cuts at: 4325 6037 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1075 678 595 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6039 6071 6039
Size: 32 6047
Tth111I GACn'n_nGTC
Cuts at: 1316 2929 1316
Size: 1613 4466
XbaI T'CTAG_A
Cuts at: 3415 3415
Size: 6079
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2815 2815
Size: 6079
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6079
XmaI C'CCGG_G
Cuts at: 1870 3404 1870
Size: 1534 4545
XmnI GAAnn'nnTTC
Cuts at: 3162 4208 3162
Size: 1046 5033
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2473 3887 276
Size: 53 83 186 936 939 1414 2468
Fragments arranged by size:
2468 1414 939 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI