Restriction map for
pFN21AE4440
(Circular) (Six-base) MAPSORT of: pFN21AE4440.seq Check: 8433 from: 1 to: 6076
pFN21AE4440 6076 bp
With 182 enzymes: SgfI *
March 28, 2012 14:01 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3886 278
Size: 53 83 186 936 2350 2468
Fragments arranged by size:
2468 2350 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3419 1937
Size: 1482 4594
Acc65I G'GTAC_C
Cuts at: 3397 3397
Size: 6076
AclI AA'CG_TT
Cuts at: 1563 4203 4576 1563
Size: 2640 373 3063
Fragments arranged by size:
3063 2640 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2252 3136 4139 5523 5791 1632
Size: 620 884 1003 1384 268 1917
Fragments arranged by size:
1917 1384 1003 884 620 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5879
AflIII A'CryG_T
Cuts at: 1203 4975 5952 1203
Size: 3772 977 1327
Fragments arranged by size:
3772 1327 977
AgeI A'CCGG_T
Cuts at: 4907 4907
Size: 6076
AhdI GACnn_n'nnGTC
Cuts at: 4805 4805
Size: 6076
AleI CACnn'nnGTG
Cuts at: 1166 2759 1166
Size: 1593 4483
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2070 1184
Size: 886 5190
AlwNI CAG_nnn'CTG
Cuts at: 2632 3304 3819 5391 2632
Size: 672 515 1572 3317
Fragments arranged by size:
3317 1572 672 515
ApaI G_GGCC'C
Cuts at: 3139 3161 3139
Size: 22 6054
ApaLI G'TGCA_C
Cuts at: 4133 5289 4133
Size: 1156 4920
ApoI r'AATT_y
Cuts at: 1493 2224 3385 3611 3763 5872 1493
Size: 731 1161 226 152 2109 1697
Fragments arranged by size:
2109 1697 1161 731 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4630 160
Size: 872 3598 1606
Fragments arranged by size:
3598 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6076
AvaI C'yCGr_G
Cuts at: 1870 1943 2738 2776 3138 3198 3248 3401 1870
Size: 73 795 38 362 60 50 153 4545
Fragments arranged by size:
4545 795 362 153 73 60 50 38
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2082 2115 2082
Size: 33 6043
BamHI G'GATC_C
Cuts at: 2199 3406 3773 2199
Size: 1207 367 4502
Fragments arranged by size:
4502 1207 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2261 3397 4852 618
Size: 325 217 627 474 1136 1455 1842
Fragments arranged by size:
1842 1455 1136 627 474 325 217
BanII G_rGCy'C
Cuts at: 1391 2241 2475 3139 3161 3395 1391
Size: 850 234 664 22 234 4072
Fragments arranged by size:
4072 850 664 234 234 22
BbeI G_GCGC'C
Cuts at: 2265 2265
Size: 6076
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5105
BbvCI CC'TCA_GC
Cuts at: 2493 2715 2859 2493
Size: 222 144 5710
Fragments arranged by size:
5710 222 144
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4267 4301 1903
Size: 34 50 34 2246 34 3678
Fragments arranged by size:
3678 2246 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2083 3968 5178 2083
Size: 1885 1210 2981
Fragments arranged by size:
2981 1885 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6076
BfrBI ATG'CAT
Cuts at: 2109 2109
Size: 6076
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2671 3326 4687
Size: 107 122 71 631 605 999 655 1361
Cuts at: 4687 136
Size: 1525
Fragments arranged by size:
1525 1361 999 655 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6071 6071
Size: 6076
BlpI GC'TnA_GC
Cuts at: 3493 3493
Size: 6076
Bme1580I G_kGCm'C
Cuts at: 1790 2278 3139 3161 4137 5293 1790
Size: 488 861 22 976 1156 2573
Fragments arranged by size:
2573 1156 976 861 488 22
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6076
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3807 4765 442
Size: 416 53 83 399 24 2390 958 1753
Fragments arranged by size:
2390 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6076
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2140 2638 3016 2140
Size: 498 378 5200
Fragments arranged by size:
5200 498 378
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2544 2742 2999 3049 3098 3205 3215 3326
Size: 1305 198 257 50 49 107 10 111
Cuts at: 3326 3356 4736 1239
Size: 30 1380 2579
Fragments arranged by size:
2579 1380 1305 257 198 111 107 50
49 30 10
Bpu10I CC'TnA_GC
Cuts at: 2493 2715 2859 2868 2493
Size: 222 144 9 5701
Fragments arranged by size:
5701 222 144 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2348 3233 4201 5066 5364 2348
Size: 885 968 865 298 3060
Fragments arranged by size:
3060 968 885 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4739 882
Size: 617 3240 2219
Fragments arranged by size:
3240 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3867 493
Size: 711 2663 2702
Fragments arranged by size:
2702 2663 711
BsaBI GATnn'nnATC
Cuts at: 6070 6070
Size: 6076
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2262 3883 4265 4900
Size: 53 83 186 936 729 1621 382 635
Cuts at: 4900 275
Size: 1451
Fragments arranged by size:
1621 1451 936 729 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2799 4509 4907 5181 5328 5739 2799
Size: 1710 398 274 147 411 3136
Fragments arranged by size:
3136 1710 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2542 2572 3299 3329 1178
Size: 30 165 30 1139 30 727 30 3925
Fragments arranged by size:
3925 1139 727 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2202 2540 2543 2546 2549 2552 2705 2708
Size: 1016 338 3 3 3 3 153 3
Cuts at: 2708 2711 2714 2717 2720 2723 2741 2876 2930
Size: 3 3 3 3 3 18 135 54
Cuts at: 2930 2933 2936 2939 3002 3005 3104 3107 3110
Size: 3 3 3 63 3 99 3 3
Cuts at: 3110 3266 3278 3281 1186
Size: 156 12 3 3981
Fragments arranged by size:
3981 1016 338 156 153 135 99 63
54 18 12 3 3 3 3 3
3 3 3 3 3 3 3 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 2144 2573 2843 2904 5279 5775 2144
Size: 429 270 61 2375 496 2445
Fragments arranged by size:
2445 2375 496 429 270 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2679 1163
Size: 573 943 4560
Fragments arranged by size:
4560 943 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2819 3526 4287 5315 1540
Size: 275 186 818 707 761 1028 2301
Fragments arranged by size:
2301 1028 818 761 707 275 186
BsiHKAI G_wGCw'C
Cuts at: 2241 2901 4137 4222 5293 2241
Size: 660 1236 85 1071 3024
Fragments arranged by size:
3024 1236 1071 660 85
BsmI GAATG_Cn'
Cuts at: 3587 3680 3587
Size: 93 5983
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2241 2278 2475 2901 3139 3161 3395
Size: 399 451 37 197 426 238 22 234
Cuts at: 3395 4137 4222 5293 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 451 426 399 238 234
197 85 37 22
BspEI T'CCGG_A
Cuts at: 2799 5739 2799
Size: 2940 3136
BspHI T'CATG_A
Cuts at: 3965 5695 3965
Size: 1730 4346
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3431 844
Size: 2587 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2970 2995 3963 1394
Size: 429 1147 25 968 3507
Fragments arranged by size:
3507 1147 968 429 25
BsrDI GCAATG_nn'
Cuts at: 65 1248 4571 4745 65
Size: 1183 3323 174 1396
Fragments arranged by size:
3323 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4720 4907 1700
Size: 110 2910 187 2869
Fragments arranged by size:
2910 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6076
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6076
BssSI C'ACGA_G
Cuts at: 4136 5148 4136
Size: 1012 5064
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6076
BstBI TT'CG_AA
Cuts at: 4880 4880
Size: 6076
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6076
BstYI r'GATC_y
Cuts at: 1972 2199 2796 3406 3773 4158 4175 5627 5713
Size: 227 597 610 367 385 17 1452 86
Cuts at: 5713 5725 6071 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 610 597 385 367 346 227
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5524
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5337
Fragments arranged by size:
5337 683 56
BtsI GCAGTG_nn'
Cuts at: 2052 2940 3592 4386 4406 2052
Size: 888 652 794 20 3722
Fragments arranged by size:
3722 888 794 652 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6076
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6041
DraI TTT'AAA
Cuts at: 3380 3380
Size: 6076
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5083 809
Size: 507 3767 1802
Fragments arranged by size:
3767 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3048 3318 3523
Size: 54 1425 50 275 18 1218 270 205
Cuts at: 3523 4412 6041 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1218 889 275 270 205 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3523 1537
Size: 275 1711 4090
Fragments arranged by size:
4090 1711 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2034 2364 2517 2682 2957 2960 4006 1353
Size: 681 330 153 165 275 3 1046 3423
Fragments arranged by size:
3423 1046 681 330 275 165 153 3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2002 4663 5037 5183 387
Size: 1064 138 413 2661 374 146 1280
Fragments arranged by size:
2661 1280 1064 413 374 146 138
EcoICRI GAG'CTC
Cuts at: 2239 2239
Size: 6076
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2252 2544 2742 2999 3049 3098 3136
Size: 393 620 292 198 257 50 49 38
Cuts at: 3136 3205 3215 3326 3356 4139 4736 5523 5791
Size: 69 10 111 30 783 597 787 268
Cuts at: 5791 1239
Size: 1524
Fragments arranged by size:
1524 787 783 620 597 393 292 268
257 198 111 69 50 49 38 30
10
EcoNI CCTnn'n_nnAGG
Cuts at: 2624 2624
Size: 6076
EcoO109I rG'GnC_Cy
Cuts at: 2454 2461 2509 2617 2650 2750 2872 3157 3158
Size: 7 48 108 33 100 122 285 1
Cuts at: 3158 2454
Size: 5372
Fragments arranged by size:
5372 285 122 108 100 48 33 7
1
EcoRI G'AATT_C
Cuts at: 3385 3763 3385
Size: 378 5698
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3145 3145
Size: 6076
FspI TGC'GCA
Cuts at: 4582 4582
Size: 6076
HaeII r_GCGC'y
Cuts at: 1678 2265 5223 1678
Size: 587 2958 2531
Fragments arranged by size:
2958 2531 587
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2263 2295 2300 2332 2363
Size: 32 135 32 691 32 5 32 31
Cuts at: 2363 2395 2801 2833 3299 3331 4717 4749 4791
Size: 32 406 32 466 32 1386 32 42
Cuts at: 4791 4823 1373
Size: 32 2626
Fragments arranged by size:
2626 1386 691 466 406 135 42 32
32 32 32 32 32 32 32 32
31 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3420 3666 669
Size: 988 281 1482 246 3079
Fragments arranged by size:
3079 1482 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6076
HpaI GTT'AAC
Cuts at: 3666 3666
Size: 6076
KasI G'GCGC_C
Cuts at: 2261 2261
Size: 6076
KpnI G_GTAC'C
Cuts at: 3401 3401
Size: 6076
MfeI C'AATT_G
Cuts at: 3675 3675
Size: 6076
MluI A'CGCG_T
Cuts at: 5952 5952
Size: 6076
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2151 2799 5190 5374 1305
Size: 846 648 2391 184 2007
Fragments arranged by size:
2391 2007 846 648 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3050 10
Size: 54 1425 343 1218 3036
Fragments arranged by size:
3036 1425 1218 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2759 4034 4393 4552 518
Size: 648 1593 1275 359 159 2042
Fragments arranged by size:
2042 1593 1275 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2018 2298 3046 3172 3355 3492 4169 5317
Size: 412 280 748 126 183 137 677 1148
Cuts at: 5317 5562 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 748 677 412 280 245 183
137 126
NarI GG'CG_CC
Cuts at: 2262 2262
Size: 6076
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5524
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6076
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6076
NotI GC'GGCC_GC
Cuts at: 3523 3523
Size: 6076
NsiI A_TGCA'T
Cuts at: 2111 2111
Size: 6076
NspI r_CATG'y
Cuts at: 1132 1229 3434 4979 1132
Size: 97 2205 1545 2229
Fragments arranged by size:
2229 2205 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2513 3161 1348
Size: 1165 648 4263
Fragments arranged by size:
4263 1165 648
PciI A'CATG_T
Cuts at: 4975 4975
Size: 6076
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3009 3027 3304 1108
Size: 1901 18 277 3880
Fragments arranged by size:
3880 1901 277 18
PfoI T'CCnGG_A
Cuts at: 2466 2466
Size: 6076
PmeI GTTT'AAAC
Cuts at: 3380 3380
Size: 6076
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4145 4177 5658 5690 4145
Size: 32 1481 32 4531
Fragments arranged by size:
4531 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2872 2872
Size: 6076
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6076
PsiI TTA'TAA
Cuts at: 3646 3646
Size: 6076
PspOMI G'GGCC_C
Cuts at: 3135 3157 3135
Size: 22 6054
PstI C_TGCA'G
Cuts at: 830 3360 3428 830
Size: 2530 68 3478
Fragments arranged by size:
3478 2530 68
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6076
PvuII CAG'CTG
Cuts at: 3046 3172 3355 3046
Size: 126 183 5767
Fragments arranged by size:
5767 183 126
SacI G_AGCT'C
Cuts at: 2241 2241
Size: 6076
SalI G'TCGA_C
Cuts at: 1936 3418 1936
Size: 1482 4594
SapI GCTCTTCn'nnn_
Cuts at: 2034 2364 2960 2034
Size: 330 596 5150
Fragments arranged by size:
5150 596 330
SbfI CC_TGCA'GG
Cuts at: 3428 3428
Size: 6076
ScaI AGT'ACT
Cuts at: 4324 6036 4324
Size: 1712 4364
SfcI C'TryA_G
Cuts at: 826 1045 2757 3356 3424 4559 5240 5431 826
Size: 219 1712 599 68 1135 681 191 1471
Fragments arranged by size:
1712 1471 1135 681 599 219 191 68
SfoI GGC'GCC
Cuts at: 2263 2263
Size: 6076
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6076
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6076
SmaI CCC'GGG
Cuts at: 1872 3140 3200 3403 1872
Size: 1268 60 203 4545
Fragments arranged by size:
4545 1268 203 60
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2327 3212 4180 5081 5343 820
Size: 197 926 384 885 968 901 262 1553
Fragments arranged by size:
1553 968 926 901 885 384 262 197
SnaBI TAC'GTA
Cuts at: 493 3867 493
Size: 3374 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6076
SphI G_CATG'C
Cuts at: 1132 3434 1132
Size: 2302 3774
SrfI GCCC'GGGC
Cuts at: 3140 3140
Size: 6076
SspI AAT'ATT
Cuts at: 5 52 4000 5
Size: 47 3948 2081
Fragments arranged by size:
3948 2081 47
StuI AGG'CCT
Cuts at: 1517 2949 1517
Size: 1432 4644
StyI C'CwwG_G
Cuts at: 513 1065 2257 2265 2382 2412 2487 2782 3003
Size: 552 1192 8 117 30 75 295 221
Cuts at: 3003 3051 3515 513
Size: 48 464 3074
Fragments arranged by size:
3074 1192 552 464 295 221 117 75
48 30 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2567 2805 4103 4120 4273 4458 4903
Size: 1255 987 238 1298 17 153 185 445
Cuts at: 4903 325
Size: 1498
Fragments arranged by size:
1498 1298 1255 987 445 238 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4322 6034 96
Size: 274 80 33 51 1443 2345 1712 138
Fragments arranged by size:
2345 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2542 2574 6036 6068 2542
Size: 32 3462 32 2550
Fragments arranged by size:
3462 2550 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6076
XbaI T'CTAG_A
Cuts at: 3412 3412
Size: 6076
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2389 2780 2389
Size: 391 5685
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6076
XmaI C'CCGG_G
Cuts at: 1870 3138 3198 3401 1870
Size: 1268 60 203 4545
Fragments arranged by size:
4545 1268 203 60
XmnI GAAnn'nnTTC
Cuts at: 4205 4205
Size: 6076
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3884 276
Size: 53 83 186 936 2350 2468
Fragments arranged by size:
2468 2350 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BsiWI BsmBI BstXI
BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV
NruI PacI PmlI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SwaI